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MACHEREY NAGEL ribonucleic acid rna extraction
Ribonucleic Acid Rna Extraction, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ribonucleic acid rna extraction/product/MACHEREY NAGEL
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ribonucleic acid rna extraction - by Bioz Stars, 2020-04
92/100 stars

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RNA Extraction:

Article Title: Fabrication of a silver particle-integrated silicone polymer-covered metal stent against sludge and biofilm formation and stent-induced tissue inflammation
Article Snippet: .. Ribonucleic acid (RNA) extraction and reverse transcription polymerase chain reaction (RT-PCR) Total RNA was isolated using a commercially available kit (Nucleospin RNAII, Macherey-Nagel, Germany), following the manufacturer’s instructions. .. Total 500 ng RNA was reverse-transcribed, and IL-10 and GAPDH transcripts were amplified using an RT-PCR PreMIX Kit (Intron, Inc., Daejon, Korea).

Amplification:

Article Title: Fabrication of a silver particle-integrated silicone polymer-covered metal stent against sludge and biofilm formation and stent-induced tissue inflammation
Article Snippet: Ribonucleic acid (RNA) extraction and reverse transcription polymerase chain reaction (RT-PCR) Total RNA was isolated using a commercially available kit (Nucleospin RNAII, Macherey-Nagel, Germany), following the manufacturer’s instructions. .. Total 500 ng RNA was reverse-transcribed, and IL-10 and GAPDH transcripts were amplified using an RT-PCR PreMIX Kit (Intron, Inc., Daejon, Korea).

Isolation:

Article Title: Fabrication of a silver particle-integrated silicone polymer-covered metal stent against sludge and biofilm formation and stent-induced tissue inflammation
Article Snippet: .. Ribonucleic acid (RNA) extraction and reverse transcription polymerase chain reaction (RT-PCR) Total RNA was isolated using a commercially available kit (Nucleospin RNAII, Macherey-Nagel, Germany), following the manufacturer’s instructions. .. Total 500 ng RNA was reverse-transcribed, and IL-10 and GAPDH transcripts were amplified using an RT-PCR PreMIX Kit (Intron, Inc., Daejon, Korea).

Size-exclusion Chromatography:

Article Title: Fabrication of a silver particle-integrated silicone polymer-covered metal stent against sludge and biofilm formation and stent-induced tissue inflammation
Article Snippet: Ribonucleic acid (RNA) extraction and reverse transcription polymerase chain reaction (RT-PCR) Total RNA was isolated using a commercially available kit (Nucleospin RNAII, Macherey-Nagel, Germany), following the manufacturer’s instructions. .. The PCR reaction was performed in a total volume of 20 μl, and denaturation was run for 35 cycles at 94 °C for 45 sec, annealing at 58 °C for 45 sec, and extension at 72 °C for 1 min, followed by final extension at 72 °C for 5 min.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Fabrication of a silver particle-integrated silicone polymer-covered metal stent against sludge and biofilm formation and stent-induced tissue inflammation
Article Snippet: .. Ribonucleic acid (RNA) extraction and reverse transcription polymerase chain reaction (RT-PCR) Total RNA was isolated using a commercially available kit (Nucleospin RNAII, Macherey-Nagel, Germany), following the manufacturer’s instructions. .. Total 500 ng RNA was reverse-transcribed, and IL-10 and GAPDH transcripts were amplified using an RT-PCR PreMIX Kit (Intron, Inc., Daejon, Korea).

CTG Assay:

Article Title: Fabrication of a silver particle-integrated silicone polymer-covered metal stent against sludge and biofilm formation and stent-induced tissue inflammation
Article Snippet: Ribonucleic acid (RNA) extraction and reverse transcription polymerase chain reaction (RT-PCR) Total RNA was isolated using a commercially available kit (Nucleospin RNAII, Macherey-Nagel, Germany), following the manufacturer’s instructions. .. The following primer sets were used for RT-PCR: IL-10 forward, 5′-TAC AGC CGG GAA GAC AAT AAC-3′; IL-10 reverse, 5′-CAC CTT GGT CTT GGA GCT TAT-3′; GAPDH forward, 5′-ACC ACA GTC CAT GCC ATC AC-3′; GAPDH reverse, 5′-TCC ACC ACC CTG TTG CTG TA-3′.

Polymerase Chain Reaction:

Article Title: Fabrication of a silver particle-integrated silicone polymer-covered metal stent against sludge and biofilm formation and stent-induced tissue inflammation
Article Snippet: Ribonucleic acid (RNA) extraction and reverse transcription polymerase chain reaction (RT-PCR) Total RNA was isolated using a commercially available kit (Nucleospin RNAII, Macherey-Nagel, Germany), following the manufacturer’s instructions. .. The PCR reaction was performed in a total volume of 20 μl, and denaturation was run for 35 cycles at 94 °C for 45 sec, annealing at 58 °C for 45 sec, and extension at 72 °C for 1 min, followed by final extension at 72 °C for 5 min.

Chloramphenicol Acetyltransferase Assay:

Article Title: Fabrication of a silver particle-integrated silicone polymer-covered metal stent against sludge and biofilm formation and stent-induced tissue inflammation
Article Snippet: Ribonucleic acid (RNA) extraction and reverse transcription polymerase chain reaction (RT-PCR) Total RNA was isolated using a commercially available kit (Nucleospin RNAII, Macherey-Nagel, Germany), following the manufacturer’s instructions. .. The following primer sets were used for RT-PCR: IL-10 forward, 5′-TAC AGC CGG GAA GAC AAT AAC-3′; IL-10 reverse, 5′-CAC CTT GGT CTT GGA GCT TAT-3′; GAPDH forward, 5′-ACC ACA GTC CAT GCC ATC AC-3′; GAPDH reverse, 5′-TCC ACC ACC CTG TTG CTG TA-3′.

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    MACHEREY NAGEL ribonucleic acid rna extraction
    Ribonucleic Acid Rna Extraction, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ribonucleic acid rna extraction/product/MACHEREY NAGEL
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ribonucleic acid rna extraction - by Bioz Stars, 2020-04
    92/100 stars
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