Structured Review

Euromedex ribonuclease inhibitor
Ribonuclease Inhibitor, supplied by Euromedex, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ribonuclease inhibitor/product/Euromedex
Average 94 stars, based on 4 article reviews
Price from $9.99 to $1999.99
ribonuclease inhibitor - by Bioz Stars, 2020-11
94/100 stars

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Related Articles

Concentration Assay:

Article Title: The role of Xist‐mediated Polycomb recruitment in the initiation of X‐chromosome inactivation
Article Snippet: .. Coverslips were incubated with the following primary antibodies diluted in blocking solution at the desired concentration (H3K27me3—Active Motif #39155 1:200; H2AK119ub—Cell Signaling #8240 1:200; JARID2—Abcam #ab48137 1:500; RING1B—Cell Signaling #5694 1:100; EZH2—Leica Microsystems #NCL‐L‐EZH2 1:200) in the presence of a ribonuclease inhibitor (0.8 μl/ml; Euromedex) for 45 min at RT (in the case of RING1B antibody, incubation lasted for 4 h). .. After three washes with PBS for 5 min, the coverslips were incubated with a secondary antibody (goat anti‐mouse or anti‐rabbit antibodies conjugated with Alexa green, red, or Cy5 fluorophores diluted 1:500) for 45 min in blocking solution supplemented with ribonuclease inhibitor (0.8 μl/ml; Euromedex).

Incubation:

Article Title: The Implication of Early Chromatin Changes in X Chromosome Inactivation
Article Snippet: .. Coverslips were incubated with primary antibodies diluted in blocking solution (anti-H4ac: 1/500; anti-H3K27ac:1/100; anti-H2AK119Ub: 1/200) in the presence of a Ribonuclease Inhibitor (0.8u/mL; Euromedex) for 45 min at room temperature. .. Coverslips were washed thrice with PBS for 5min and subsequently incubated with secondary antibody solution (goat anti-mouse or rabbit conjugated with Alexa fluorophores; 1/500; supplemented with Ribonuclease Inhibitor [0.8u/mL; Euromedex]) for 45 min at room temperature.

Article Title: The role of Xist‐mediated Polycomb recruitment in the initiation of X‐chromosome inactivation
Article Snippet: .. After three washes with PBS for 5 min, the coverslips were incubated with a secondary antibody (goat anti‐mouse or anti‐rabbit antibodies conjugated with Alexa green, red, or Cy5 fluorophores diluted 1:500) for 45 min in blocking solution supplemented with ribonuclease inhibitor (0.8 μl/ml; Euromedex). ..

Article Title: The role of Xist‐mediated Polycomb recruitment in the initiation of X‐chromosome inactivation
Article Snippet: .. Coverslips were incubated with the following primary antibodies diluted in blocking solution at the desired concentration (H3K27me3—Active Motif #39155 1:200; H2AK119ub—Cell Signaling #8240 1:200; JARID2—Abcam #ab48137 1:500; RING1B—Cell Signaling #5694 1:100; EZH2—Leica Microsystems #NCL‐L‐EZH2 1:200) in the presence of a ribonuclease inhibitor (0.8 μl/ml; Euromedex) for 45 min at RT (in the case of RING1B antibody, incubation lasted for 4 h). .. After three washes with PBS for 5 min, the coverslips were incubated with a secondary antibody (goat anti‐mouse or anti‐rabbit antibodies conjugated with Alexa green, red, or Cy5 fluorophores diluted 1:500) for 45 min in blocking solution supplemented with ribonuclease inhibitor (0.8 μl/ml; Euromedex).

Article Title: Tracking H3K27me3 and H4K20me1 dynamics during XCI reveals similarities in recruitment mechanism
Article Snippet: .. After three washes with PBS for 5 min, the coverslips were incubated with a secondary antibody (antilJrabbit antibodies conjugated with Alexa fluorophores diluted 1:500) for 45 min in blocking solution supplemented with ribonuclease inhibitor (0.8 μl/ml; Euromedex). ..

Article Title: Tracking H3K27me3 and H4K20me1 dynamics during XCI reveals similarities in recruitment mechanism
Article Snippet: .. Coverslips were incubated with the H4K20me1 primary antibody (Abcam, abb9051) diluted in blocking solution in the presence of a ribonuclease inhibitor (0.8 μl/ml; Euromedex) for 45 min at RT. .. After three washes with PBS for 5 min, the coverslips were incubated with a secondary antibody (antilJrabbit antibodies conjugated with Alexa fluorophores diluted 1:500) for 45 min in blocking solution supplemented with ribonuclease inhibitor (0.8 μl/ml; Euromedex).

Blocking Assay:

Article Title: The Implication of Early Chromatin Changes in X Chromosome Inactivation
Article Snippet: .. Coverslips were incubated with primary antibodies diluted in blocking solution (anti-H4ac: 1/500; anti-H3K27ac:1/100; anti-H2AK119Ub: 1/200) in the presence of a Ribonuclease Inhibitor (0.8u/mL; Euromedex) for 45 min at room temperature. .. Coverslips were washed thrice with PBS for 5min and subsequently incubated with secondary antibody solution (goat anti-mouse or rabbit conjugated with Alexa fluorophores; 1/500; supplemented with Ribonuclease Inhibitor [0.8u/mL; Euromedex]) for 45 min at room temperature.

Article Title: The role of Xist‐mediated Polycomb recruitment in the initiation of X‐chromosome inactivation
Article Snippet: .. After three washes with PBS for 5 min, the coverslips were incubated with a secondary antibody (goat anti‐mouse or anti‐rabbit antibodies conjugated with Alexa green, red, or Cy5 fluorophores diluted 1:500) for 45 min in blocking solution supplemented with ribonuclease inhibitor (0.8 μl/ml; Euromedex). ..

Article Title: The role of Xist‐mediated Polycomb recruitment in the initiation of X‐chromosome inactivation
Article Snippet: .. Coverslips were incubated with the following primary antibodies diluted in blocking solution at the desired concentration (H3K27me3—Active Motif #39155 1:200; H2AK119ub—Cell Signaling #8240 1:200; JARID2—Abcam #ab48137 1:500; RING1B—Cell Signaling #5694 1:100; EZH2—Leica Microsystems #NCL‐L‐EZH2 1:200) in the presence of a ribonuclease inhibitor (0.8 μl/ml; Euromedex) for 45 min at RT (in the case of RING1B antibody, incubation lasted for 4 h). .. After three washes with PBS for 5 min, the coverslips were incubated with a secondary antibody (goat anti‐mouse or anti‐rabbit antibodies conjugated with Alexa green, red, or Cy5 fluorophores diluted 1:500) for 45 min in blocking solution supplemented with ribonuclease inhibitor (0.8 μl/ml; Euromedex).

Article Title: Tracking H3K27me3 and H4K20me1 dynamics during XCI reveals similarities in recruitment mechanism
Article Snippet: .. After three washes with PBS for 5 min, the coverslips were incubated with a secondary antibody (antilJrabbit antibodies conjugated with Alexa fluorophores diluted 1:500) for 45 min in blocking solution supplemented with ribonuclease inhibitor (0.8 μl/ml; Euromedex). ..

Article Title: Tracking H3K27me3 and H4K20me1 dynamics during XCI reveals similarities in recruitment mechanism
Article Snippet: .. Coverslips were incubated with the H4K20me1 primary antibody (Abcam, abb9051) diluted in blocking solution in the presence of a ribonuclease inhibitor (0.8 μl/ml; Euromedex) for 45 min at RT. .. After three washes with PBS for 5 min, the coverslips were incubated with a secondary antibody (antilJrabbit antibodies conjugated with Alexa fluorophores diluted 1:500) for 45 min in blocking solution supplemented with ribonuclease inhibitor (0.8 μl/ml; Euromedex).

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  • 94
    Euromedex ribonuclease inhibitor
    Ribonuclease Inhibitor, supplied by Euromedex, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ribonuclease inhibitor/product/Euromedex
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    ribonuclease inhibitor - by Bioz Stars, 2020-11
    94/100 stars
      Buy from Supplier

    92
    Euromedex rnasin
    Rnasin, supplied by Euromedex, used in various techniques. Bioz Stars score: 92/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnasin/product/Euromedex
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rnasin - by Bioz Stars, 2020-11
    92/100 stars
      Buy from Supplier

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