Structured Review

Amresco ribonuclease inhibitor
Ribonuclease Inhibitor, supplied by Amresco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ribonuclease inhibitor/product/Amresco
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
ribonuclease inhibitor - by Bioz Stars, 2021-06
86/100 stars

Images

Related Articles

Centrifugation:

Article Title: Export of autotransported proteins proceeds through an oligomeric ring shaped byC-terminal domains
Article Snippet: The expression of FvHβ is under tac promoter control in pVFvHβ. .. Escherichia coli UT5600 (pHEβ) cells were grown in 3 l of 2×YT medium at 30°C and induced with 1 mM IPTG for 16 h. Cells were subsequently harvested by centrifugation (4000 g , 10 min) and resuspended in buffer TN (20 mM Tris–HCl pH 8.0, 10 mM NaCl) containing DNase I (0.1 mg/ml; Roche), pancreatic RNase A (0.1 mg/ml; Amresco), 0.1 mM phenylmethylsulfonyl flouride (PMSF) and a cocktail of protease inhibitors (Complete EDTA-free® ; Roche). .. The suspension of cells was passed through a French press (Sim-Aminco® ; Electronic Instruments) at 14 000 p.s.i. followed by a short centrifugation (4000 g , 10 min) to discard non-lysed cells.

Polymerase Chain Reaction:

Article Title: Identification of determinants for tRNA substrate recognition by Escherichia coli C/U34 2′-O-methyltransferase
Article Snippet: .. Materials [Methyl-3 H] SAM (78.0 Ci/mmol) was purchased from PerkinElmer Inc. (Waltham, MA, USA); [3 H] DMAPP (20.0 Ci/mmol) was obtained from BIOTREND Chemicals (USA); SAM was purchased from NEW ENGLAND Biolabs Inc..; Dimethylallyl diphosphate (DMAPP), dithiothreitol (DTT), NTPs, 5′-GMP, pyrophosphate, Tris-base, β-mercaptoethanol (β-Me), MgCl2 , NaCl and KCl were purchased from Sigma-Aldrich Co. LLC. (St. Louis, MO, USA); Isopropyl β-D-thiogalactoside (IPTG) was obtained from AMRESCO (OH, USA); Nitrocellulose membranes (0.22 μm) were purchased from Merck Millipore (Darmstadt, Germany); Primers for PCR were synthesized by BioSune (Shanghai, China); Nickel-nitrilotriacetic (Ni-NTA) Superflow was purchased from Qiagen Inc.. (Germany); KOD-plus mutagenesis kit, Pyrobest DNA polymerase and the dNTP mixture were obtained from Takara (Japan); T4 polynucleotide kinase, T4 DNA ligase, Ribonuclease inhibitor and all restriction endonucleases were obtained from Fermentas/Thermo Scientific (MA, USA); The E. coli JW3581-1 and JW4129-2, strains in which the gene of TrmL and MiaA were deleted respectively, were purchased from the E. coli genetic stock center (Yale University, New Haven, CT, USA); PrepHT 300SB-C18 was purchased from Agilent Technologies (CA, USA). .. Preparation of tRNAs and mutagenesis The genes encoding Ec tRNAs including Ec tRNALeu CAA , Ec tRNALeu UAA and Ec tRNAPhe GAA were amplified from the genome of E. coli MG1655.

Article Title: The nestin-expressing and non-expressing neurons in rat basal forebrain display different electrophysiological properties and project to hippocampus
Article Snippet: .. The pipette was then quickly removed from the slice, and its content was expelled into a 0.2 ml PCR tube containing 5 μl of 20 mM dithiothreitol (DTT, Amresco, USA), 20 U ribonuclease inhibitor and 20 picomoles of random hexamers (volume was measured and adjusted to 10 μl with nuclease-free water and quickly cooled on ice). ..

Synthesized:

Article Title: Identification of determinants for tRNA substrate recognition by Escherichia coli C/U34 2′-O-methyltransferase
Article Snippet: .. Materials [Methyl-3 H] SAM (78.0 Ci/mmol) was purchased from PerkinElmer Inc. (Waltham, MA, USA); [3 H] DMAPP (20.0 Ci/mmol) was obtained from BIOTREND Chemicals (USA); SAM was purchased from NEW ENGLAND Biolabs Inc..; Dimethylallyl diphosphate (DMAPP), dithiothreitol (DTT), NTPs, 5′-GMP, pyrophosphate, Tris-base, β-mercaptoethanol (β-Me), MgCl2 , NaCl and KCl were purchased from Sigma-Aldrich Co. LLC. (St. Louis, MO, USA); Isopropyl β-D-thiogalactoside (IPTG) was obtained from AMRESCO (OH, USA); Nitrocellulose membranes (0.22 μm) were purchased from Merck Millipore (Darmstadt, Germany); Primers for PCR were synthesized by BioSune (Shanghai, China); Nickel-nitrilotriacetic (Ni-NTA) Superflow was purchased from Qiagen Inc.. (Germany); KOD-plus mutagenesis kit, Pyrobest DNA polymerase and the dNTP mixture were obtained from Takara (Japan); T4 polynucleotide kinase, T4 DNA ligase, Ribonuclease inhibitor and all restriction endonucleases were obtained from Fermentas/Thermo Scientific (MA, USA); The E. coli JW3581-1 and JW4129-2, strains in which the gene of TrmL and MiaA were deleted respectively, were purchased from the E. coli genetic stock center (Yale University, New Haven, CT, USA); PrepHT 300SB-C18 was purchased from Agilent Technologies (CA, USA). .. Preparation of tRNAs and mutagenesis The genes encoding Ec tRNAs including Ec tRNALeu CAA , Ec tRNALeu UAA and Ec tRNAPhe GAA were amplified from the genome of E. coli MG1655.

Mutagenesis:

Article Title: Identification of determinants for tRNA substrate recognition by Escherichia coli C/U34 2′-O-methyltransferase
Article Snippet: .. Materials [Methyl-3 H] SAM (78.0 Ci/mmol) was purchased from PerkinElmer Inc. (Waltham, MA, USA); [3 H] DMAPP (20.0 Ci/mmol) was obtained from BIOTREND Chemicals (USA); SAM was purchased from NEW ENGLAND Biolabs Inc..; Dimethylallyl diphosphate (DMAPP), dithiothreitol (DTT), NTPs, 5′-GMP, pyrophosphate, Tris-base, β-mercaptoethanol (β-Me), MgCl2 , NaCl and KCl were purchased from Sigma-Aldrich Co. LLC. (St. Louis, MO, USA); Isopropyl β-D-thiogalactoside (IPTG) was obtained from AMRESCO (OH, USA); Nitrocellulose membranes (0.22 μm) were purchased from Merck Millipore (Darmstadt, Germany); Primers for PCR were synthesized by BioSune (Shanghai, China); Nickel-nitrilotriacetic (Ni-NTA) Superflow was purchased from Qiagen Inc.. (Germany); KOD-plus mutagenesis kit, Pyrobest DNA polymerase and the dNTP mixture were obtained from Takara (Japan); T4 polynucleotide kinase, T4 DNA ligase, Ribonuclease inhibitor and all restriction endonucleases were obtained from Fermentas/Thermo Scientific (MA, USA); The E. coli JW3581-1 and JW4129-2, strains in which the gene of TrmL and MiaA were deleted respectively, were purchased from the E. coli genetic stock center (Yale University, New Haven, CT, USA); PrepHT 300SB-C18 was purchased from Agilent Technologies (CA, USA). .. Preparation of tRNAs and mutagenesis The genes encoding Ec tRNAs including Ec tRNALeu CAA , Ec tRNALeu UAA and Ec tRNAPhe GAA were amplified from the genome of E. coli MG1655.

Purification:

Article Title: In vitro inhibition of feline coronavirus replication by small interfering RNAs
Article Snippet: 2.6.2 cDNA synthesis cDNA was generated from purified RNA using Superscript® III (Invitrogen), a modified Moloney Murine Leukaemia Virus reverse transcriptase in a final reaction volume of 20 μl. .. The reactions consisted of 1 μg purified RNA, 100 ng random primers (Promega, Alexandria, NSW, Australia), 2.5 μmol of each dNTP (Promega), and RNase free water (Amresco) to a final volume of 14.5 μl. .. Samples were incubated at 65 °C for 5 min to denature RNA and then cooled on ice for 1 min. To this was added a mix consisting of 4.5 μl First Stand Buffer (Invitrogen), 20 units RNasin Plus RNase Inhibitor (Promega), and 100 units Superscript III.

Transferring:

Article Title: The nestin-expressing and non-expressing neurons in rat basal forebrain display different electrophysiological properties and project to hippocampus
Article Snippet: .. The pipette was then quickly removed from the slice, and its content was expelled into a 0.2 ml PCR tube containing 5 μl of 20 mM dithiothreitol (DTT, Amresco, USA), 20 U ribonuclease inhibitor and 20 picomoles of random hexamers (volume was measured and adjusted to 10 μl with nuclease-free water and quickly cooled on ice). ..

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Amresco rnase inhibitor
    Rnase Inhibitor, supplied by Amresco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase inhibitor/product/Amresco
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rnase inhibitor - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    86
    Amresco lysis buffer
    Lysis Buffer, supplied by Amresco, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lysis buffer/product/Amresco
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lysis buffer - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    Image Search Results