Structured Review

Horizon Discovery rgs12
<t>RGS12</t> coordinates a Ras/Raf/MEK/ERK complex and enhances signaling to ERK. ( A ) CHO-K1 cells with endogenous PDGFβR were transfected with ERK2–GFP and either empty vector or increasing amounts of HA-RGS12 vector, serum-starved overnight,
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1) Product Images from "Selective role for RGS12 as a Ras/Raf/MEK scaffold in nerve growth factor-mediated differentiation"

Article Title: Selective role for RGS12 as a Ras/Raf/MEK scaffold in nerve growth factor-mediated differentiation

Journal: The EMBO Journal

doi: 10.1038/sj.emboj.7601659

RGS12 coordinates a Ras/Raf/MEK/ERK complex and enhances signaling to ERK. ( A ) CHO-K1 cells with endogenous PDGFβR were transfected with ERK2–GFP and either empty vector or increasing amounts of HA-RGS12 vector, serum-starved overnight,
Figure Legend Snippet: RGS12 coordinates a Ras/Raf/MEK/ERK complex and enhances signaling to ERK. ( A ) CHO-K1 cells with endogenous PDGFβR were transfected with ERK2–GFP and either empty vector or increasing amounts of HA-RGS12 vector, serum-starved overnight,

Techniques Used: Transfection, Plasmid Preparation

RGS12 and its isolated tandem RBDs preferentially interact with activated H-Ras. ( A, B ) HEK 293T cells were cotransfected with myc-RGS12 and indicated HA-Ras plasmids. Anti-myc was used to immunoprecipitate RGS12 and anti-HA used to detect associated
Figure Legend Snippet: RGS12 and its isolated tandem RBDs preferentially interact with activated H-Ras. ( A, B ) HEK 293T cells were cotransfected with myc-RGS12 and indicated HA-Ras plasmids. Anti-myc was used to immunoprecipitate RGS12 and anti-HA used to detect associated

Techniques Used: Isolation

Prolonged ERK activation by NGF is reduced upon RGS12 depletion, expressed RGS12 is punctate and co-incident with endosomal markers, and translocation of endogenous RGS12 from membranes is regulated by NGF. ( A ) PC12 cells were transfected with non-specific
Figure Legend Snippet: Prolonged ERK activation by NGF is reduced upon RGS12 depletion, expressed RGS12 is punctate and co-incident with endosomal markers, and translocation of endogenous RGS12 from membranes is regulated by NGF. ( A ) PC12 cells were transfected with non-specific

Techniques Used: Activation Assay, Translocation Assay, Transfection

RGS12 is critical for NGF-mediated and MAPK-dependent neurite outgrowth in PC12 cells. ( A ) PC12 lysates were immunoprecipitated with beads alone (no Ab), or anti-H-Ras, anti-Rap-1, anti-B-Raf, or anti-RGS12 antibodies. Anti-RGS12 was used to detect associated
Figure Legend Snippet: RGS12 is critical for NGF-mediated and MAPK-dependent neurite outgrowth in PC12 cells. ( A ) PC12 lysates were immunoprecipitated with beads alone (no Ab), or anti-H-Ras, anti-Rap-1, anti-B-Raf, or anti-RGS12 antibodies. Anti-RGS12 was used to detect associated

Techniques Used: Immunoprecipitation

RGS12 forms a multiprotein complex containing TrkA and shows selectivity for TrkA signaling versus FGFR signaling. ( A ) HEK 293T cells were cotransfected with myc-RGS12, and either empty vector, FLAG-TrkA, or HA-FGFR1 plasmids. Anti-FLAG or anti-HA antibody
Figure Legend Snippet: RGS12 forms a multiprotein complex containing TrkA and shows selectivity for TrkA signaling versus FGFR signaling. ( A ) HEK 293T cells were cotransfected with myc-RGS12, and either empty vector, FLAG-TrkA, or HA-FGFR1 plasmids. Anti-FLAG or anti-HA antibody

Techniques Used: Plasmid Preparation

Components of the mitogen-activated protein kinase (MAPK) cascade identified as RGS12 interactors. ( A ) RGS12 architecture. Numbering denotes amino-acid boundaries of domains within rat RGS12. Parentheses denote baits used in yeast two-hybrid screens against
Figure Legend Snippet: Components of the mitogen-activated protein kinase (MAPK) cascade identified as RGS12 interactors. ( A ) RGS12 architecture. Numbering denotes amino-acid boundaries of domains within rat RGS12. Parentheses denote baits used in yeast two-hybrid screens against

Techniques Used:

RGS12 is critical for NGF-mediated axonal growth in primary mouse DRG neurons. ( A ) N1E-115 mouse neuroblastoma cells were transfected with non-specific or mouse RGS12-directed siRNA and lysed 72 h post-transection for immunoblotting of RGS12 and actin.
Figure Legend Snippet: RGS12 is critical for NGF-mediated axonal growth in primary mouse DRG neurons. ( A ) N1E-115 mouse neuroblastoma cells were transfected with non-specific or mouse RGS12-directed siRNA and lysed 72 h post-transection for immunoblotting of RGS12 and actin.

Techniques Used: Transfection