revertaid moloney murine leukemia virus m mulv reverse transcriptase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher revertaid moloney murine leukemia virus m mulv reverse transcriptase
    Revertaid Moloney Murine Leukemia Virus M Mulv Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/revertaid moloney murine leukemia virus m mulv reverse transcriptase/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    revertaid moloney murine leukemia virus m mulv reverse transcriptase - by Bioz Stars, 2020-05
    85/100 stars

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    Sequencing:

    Article Title: A Novel Mycovirus That Is Related to the Human Pathogen Hepatitis E Virus and Rubi-Like Viruses ▿ and Rubi-Like Viruses ▿ †
    Article Snippet: .. Denatured dsRNA was reverse transcribed using RevertAid Moloney murine leukemia virus (M-MuLV) reverse transcriptase (Fermentas) and a sequence-specific reverse primer and incubated for 60 min at 45°C. .. After RT, the mixture was treated with RNase H (1 U at 37°C for 30 min; TaKaRa), and 2% of the reaction volume was used for PCR amplification with the pertinent forward and reverse primers, GC buffer, and LA Taq DNA polymerase (TaKaRa).

    Polymerase Chain Reaction:

    Article Title: Mutation of a Broadly Conserved Operon (RL3499-RL3502) from Rhizobium leguminosarum Biovar viciae Causes Defects in Cell Morphology and Envelope Integrity ▿ Causes Defects in Cell Morphology and Envelope Integrity ▿ †
    Article Snippet: .. Samples were snap cooled on ice and 2 μl of 10 mM deoxynucleoside triphosphates (dNTPs) was added, followed by incubation at 37°C for 5 min. One microliter of RevertAid Moloney murine leukemia virus (M-MuLV) reverse transcriptase (Fermentas) was added, and samples were incubated at 42°C for 50 min, followed by heat inactivation at 70°C for 15 min. PCR mixtures contained 1× reaction buffer, 2 mM MgSO4, 0.2 mM dNTPs, 2 μM forward primer, 0.12 μM reverse primer, and 1 U Taq DNA polymerase (UBI, Calgary, Alberta, Canada). ..

    Random Hexamer Labeling:

    Article Title: Gene Expression and Physiological Role of Pseudomonas aeruginosa Methionine Sulfoxide Reductases during Oxidative Stress
    Article Snippet: .. First-strand cDNA synthesis was accomplished using RevertAid Moloney murine leukemia virus (M-MuLV) reverse transcriptase (Fermentas, Lithuania) with random hexamer primers and total RNA (1 μg). .. Endpoint reverse transcription-PCR (RT-PCR) was performed to preliminarily determine the expression levels of msrA and msrB using primers BT3002 (5′-ACAGGTGCTGTTCGGCATGG-3′) and BT3003 (5′-AGTTCGGCCTGGAAGCTCTC-3′) for msrA and primers BT2203 (5′-ATCTATCATTGCACCTGCTG-3′) and BT2204 (5′-GGAAGACGTGCCCCAGGTG3′) for msrB and 10 ng of cDNA as a template for 25 cycles, as follows: 95°C for 15 s, 55°C for 15 s, and 72°C for 15 s. The 16S rRNA gene, amplified by RT-PCR using primers BT2781 (5′-GCCCGCACAAGCGGTGGAG-3′) and BT2782 (5′-ACGTCATCCCCACCTTCCT-3′), was used as an internal control to normalize cDNA samples.

    Incubation:

    Article Title: A Novel Mycovirus That Is Related to the Human Pathogen Hepatitis E Virus and Rubi-Like Viruses ▿ and Rubi-Like Viruses ▿ †
    Article Snippet: .. Denatured dsRNA was reverse transcribed using RevertAid Moloney murine leukemia virus (M-MuLV) reverse transcriptase (Fermentas) and a sequence-specific reverse primer and incubated for 60 min at 45°C. .. After RT, the mixture was treated with RNase H (1 U at 37°C for 30 min; TaKaRa), and 2% of the reaction volume was used for PCR amplification with the pertinent forward and reverse primers, GC buffer, and LA Taq DNA polymerase (TaKaRa).

    Article Title: Mutation of a Broadly Conserved Operon (RL3499-RL3502) from Rhizobium leguminosarum Biovar viciae Causes Defects in Cell Morphology and Envelope Integrity ▿ Causes Defects in Cell Morphology and Envelope Integrity ▿ †
    Article Snippet: .. Samples were snap cooled on ice and 2 μl of 10 mM deoxynucleoside triphosphates (dNTPs) was added, followed by incubation at 37°C for 5 min. One microliter of RevertAid Moloney murine leukemia virus (M-MuLV) reverse transcriptase (Fermentas) was added, and samples were incubated at 42°C for 50 min, followed by heat inactivation at 70°C for 15 min. PCR mixtures contained 1× reaction buffer, 2 mM MgSO4, 0.2 mM dNTPs, 2 μM forward primer, 0.12 μM reverse primer, and 1 U Taq DNA polymerase (UBI, Calgary, Alberta, Canada). ..

    Quantitative RT-PCR:

    Article Title: CalA, a Cyanobacterial AbrB Protein, Interacts with the Upstream Region of hypC and Acts as a Repressor of Its Transcription in the Cyanobacterium Nostoc sp. Strain PCC 7120 ▿ sp. Strain PCC 7120 ▿ †
    Article Snippet: .. In RT-qPCR 1 μg total RNA from each sample was used for cDNA synthesis using RevertAid Moloney murine leukemia virus (M-MuLV) reverse transcriptase according to the manufacturer's instructions (Fermentas). .. To verify primer specificity, PCR products were cloned using the TOPO-TA cloning kit (Invitrogen) and sequenced.

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    Thermo Fisher revertaid h minus m mulv reverse transcriptase
    Revertaid H Minus M Mulv Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher recombinant moloney murine leukemia virus reverse transcriptase
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    Thermo Fisher moloney murine leukemia virus reverse transcriptase
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