revertaid moloney murine leukaemia virus reverse transcriptase kit  (Thermo Fisher)


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    Name:
    RevertAid RT Reverse Transcription Kit
    Description:
    Thermo Scientific RevertAid RT Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates The kit uses RevertAid Reverse Transcriptase RT which has lower RNase H activity compared to AMV reverse transcriptase The enzyme maintains activity at 42 50°C and is suitable for synthesis of cDNA up to 13 kb The recombinant Thermo Scientific RiboLock RNase Inhibitor supplied with the kit effectively protects RNA from degradation at temperatures up to 55°C First strand cDNA synthesized with this system can be directly used as a template in PCR or real time PCR It is also ideal as a first step for second strand cDNA synthesis or linear RNA amplification Radioactively and non radioactively labeled nucleotides can be incorporated into first strand cDNA for use as a probe in hybridization experiments including microarrays Highlights• Full length first strand cDNA up to 13 kb• Optimum reaction temperature 42°C• Complete kit all the components for the RT reaction are includedApplications• First strand cDNA synthesis for RT PCR and RT qPCR• Construction of full length cDNA libraries• Antisense RNA synthesis
    Catalog Number:
    k1691
    Price:
    None
    Applications:
    Cloning|cDNA Libraries & Library Construction
    Category:
    Kits and Assays
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    Structured Review

    Thermo Fisher revertaid moloney murine leukaemia virus reverse transcriptase kit
    Thermo Scientific RevertAid RT Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates The kit uses RevertAid Reverse Transcriptase RT which has lower RNase H activity compared to AMV reverse transcriptase The enzyme maintains activity at 42 50°C and is suitable for synthesis of cDNA up to 13 kb The recombinant Thermo Scientific RiboLock RNase Inhibitor supplied with the kit effectively protects RNA from degradation at temperatures up to 55°C First strand cDNA synthesized with this system can be directly used as a template in PCR or real time PCR It is also ideal as a first step for second strand cDNA synthesis or linear RNA amplification Radioactively and non radioactively labeled nucleotides can be incorporated into first strand cDNA for use as a probe in hybridization experiments including microarrays Highlights• Full length first strand cDNA up to 13 kb• Optimum reaction temperature 42°C• Complete kit all the components for the RT reaction are includedApplications• First strand cDNA synthesis for RT PCR and RT qPCR• Construction of full length cDNA libraries• Antisense RNA synthesis
    https://www.bioz.com/result/revertaid moloney murine leukaemia virus reverse transcriptase kit/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    revertaid moloney murine leukaemia virus reverse transcriptase kit - by Bioz Stars, 2021-01
    99/100 stars

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    Related Articles

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Functional validation of CHMP7 as an ADHD risk gene
    Article Snippet: .. Reverse transcription-PCR RT-PCR was utilised to determine when chmp7 is expressed. cDNA synthesis was performed on RNA extracted from wildtype embryos at the 8- and 16-somite stages, 1, 1.5, 2, 3, 4, and 5 dpf. .. Total RNA was isolated using TRIzol® reagent as described by the manufacturer (Sigma, St. Louis, MO, USA) and treated with DNAse (Promega) to remove possible DNA contamination.

    Article Title: Optimized Approaches for the Induction of Putative Canine Induced Pluripotent Stem Cells from Old Fibroblasts Using Synthetic RNAs
    Article Snippet: .. Gene Expression Analysis of Putative ciPSCs by Reverse Transcription PCR (RT-PCR) The expression levels of RPL13A (a reference gene), VEE-hOct4 , VEE-hKlf4 , VEE-hSox2 , VEE-hGlis1 , Rex1, Oct4 , and Nanog mRNA in ciPSCs were analyzed by RT-PCR. .. All the cDNA samples of ciPSC-like colonies were stored at −80 °C until PCR analysis.

    Polymerase Chain Reaction:

    Article Title: Optimized Approaches for the Induction of Putative Canine Induced Pluripotent Stem Cells from Old Fibroblasts Using Synthetic RNAs
    Article Snippet: .. Gene Expression Analysis of Putative ciPSCs by Reverse Transcription PCR (RT-PCR) The expression levels of RPL13A (a reference gene), VEE-hOct4 , VEE-hKlf4 , VEE-hSox2 , VEE-hGlis1 , Rex1, Oct4 , and Nanog mRNA in ciPSCs were analyzed by RT-PCR. .. All the cDNA samples of ciPSC-like colonies were stored at −80 °C until PCR analysis.

    Expressing:

    Article Title: Optimized Approaches for the Induction of Putative Canine Induced Pluripotent Stem Cells from Old Fibroblasts Using Synthetic RNAs
    Article Snippet: .. Gene Expression Analysis of Putative ciPSCs by Reverse Transcription PCR (RT-PCR) The expression levels of RPL13A (a reference gene), VEE-hOct4 , VEE-hKlf4 , VEE-hSox2 , VEE-hGlis1 , Rex1, Oct4 , and Nanog mRNA in ciPSCs were analyzed by RT-PCR. .. All the cDNA samples of ciPSC-like colonies were stored at −80 °C until PCR analysis.

    Synthesized:

    Article Title: Involvement of CD147 on multidrug resistance through the regulation of P-glycoprotein expression in K562/ADR leukemic cell line
    Article Snippet: .. Single-stranded cDNA was synthesized by reverse transcription from 1 μg of total RNA using the RevertAid strand cDNA Synthesis Kit (Thermo Scientific, USA). .. Then, 1 μg cDNA was amplified by PCR using a DreamTag Green PCR Master Mix (Thermo ScientificTM).

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    Thermo Fisher m mlv reverse transcriptase
    M Mlv Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2784 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m mlv reverse transcriptase/product/Thermo Fisher
    Average 99 stars, based on 2784 article reviews
    Price from $9.99 to $1999.99
    m mlv reverse transcriptase - by Bioz Stars, 2021-01
    99/100 stars
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