revertaid h minus m mulv reverse transcriptase  (Thermo Fisher)


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    Name:
    RevertAid H Minus Reverse Transcriptase 200 U µL
    Description:
    Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M MuLV RT The enzyme possesses an RNA dependent and DNA dependent polymerase activity but lacks RNase H activity due to a point mutation in the RNase H domain RevertAid H Minus Reverse Transcriptase does not degrade RNA in RNA DNA hybrids during synthesis of the first strand cDNA and therefore high yields of full length cDNA from long templates are obtained RevertAid H Minus Reverse Transcriptase maintains activity over a wide temperature range 42 to 50°C Highlights• High yields of full length first strand cDNA up to 13 kb• Optimum activity at 42 to 45°C• Active up to 55°C• Incorporates modified nucleotides e g Cy3 Cy5 rhodamine aminoallyl fluorescein labeled nucleotides Applications• First strand cDNA synthesis for RT PCR and real time RT PCR• Reverse transcription at elevated temperatures to reduce effects of secondary structure• Synthesis of cDNA for cloning and expression• Generation of labeled cDNA probes for microarrays• DNA labeling• Analysis of RNA by primer extensionRelated ProductsRevertAid H Minus Reverse Transcriptase 200 U µL
    Catalog Number:
    ep0451
    Price:
    None
    Applications:
    Cloning|PCR & Real-Time PCR|RT-PCR|Reverse Transcription|Two-Step RT-PCR|cDNA Libraries & Library Construction
    Category:
    Proteins Enzymes Peptides
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    Structured Review

    Thermo Fisher revertaid h minus m mulv reverse transcriptase
    Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M MuLV RT The enzyme possesses an RNA dependent and DNA dependent polymerase activity but lacks RNase H activity due to a point mutation in the RNase H domain RevertAid H Minus Reverse Transcriptase does not degrade RNA in RNA DNA hybrids during synthesis of the first strand cDNA and therefore high yields of full length cDNA from long templates are obtained RevertAid H Minus Reverse Transcriptase maintains activity over a wide temperature range 42 to 50°C Highlights• High yields of full length first strand cDNA up to 13 kb• Optimum activity at 42 to 45°C• Active up to 55°C• Incorporates modified nucleotides e g Cy3 Cy5 rhodamine aminoallyl fluorescein labeled nucleotides Applications• First strand cDNA synthesis for RT PCR and real time RT PCR• Reverse transcription at elevated temperatures to reduce effects of secondary structure• Synthesis of cDNA for cloning and expression• Generation of labeled cDNA probes for microarrays• DNA labeling• Analysis of RNA by primer extensionRelated ProductsRevertAid H Minus Reverse Transcriptase 200 U µL
    https://www.bioz.com/result/revertaid h minus m mulv reverse transcriptase/product/Thermo Fisher
    Average 99 stars, based on 78 article reviews
    Price from $9.99 to $1999.99
    revertaid h minus m mulv reverse transcriptase - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Random Hexamer Labeling:

    Article Title: Seasonal plasticity in photoprotection modulates UV‐induced hsp gene expression in copepods from a clear lake
    Article Snippet: .. For first‐strand complementary DNA (cDNA) synthesis, 450 ng total copepod RNA and random hexamer primers (#S0142, Thermo Scientific) were used. cDNA was synthesized using MMLV H minus reverse transcriptase (#EP0452, Thermo Scientific) following the manufacturer's protocol with slight modifications by using less reverse transcriptase. ..

    Synthesized:

    Article Title: Seasonal plasticity in photoprotection modulates UV‐induced hsp gene expression in copepods from a clear lake
    Article Snippet: .. For first‐strand complementary DNA (cDNA) synthesis, 450 ng total copepod RNA and random hexamer primers (#S0142, Thermo Scientific) were used. cDNA was synthesized using MMLV H minus reverse transcriptase (#EP0452, Thermo Scientific) following the manufacturer's protocol with slight modifications by using less reverse transcriptase. ..

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    Thermo Fisher revertaid h minus moloney murine leukemia virus rt
    Revertaid H Minus Moloney Murine Leukemia Virus Rt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 311 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/revertaid h minus moloney murine leukemia virus rt/product/Thermo Fisher
    Average 99 stars, based on 311 article reviews
    Price from $9.99 to $1999.99
    revertaid h minus moloney murine leukemia virus rt - by Bioz Stars, 2020-07
    99/100 stars
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