revertaid h minus m mulv reverse transcriptase first strand cdna synthesis kit  (Thermo Fisher)


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    Name:
    RevertAid H Minus First Strand cDNA Synthesis Kit
    Description:
    Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates The kit includes RevertAid H Minus Reverse Transcriptase which has a point mutation that completely eliminates RNase H activity Therefore it does not degrade RNA in RNA DNA hybrids during synthesis of the first strand cDNA Highlights• High yields of full length first strand cDNA up to 13 kb• Increased reaction temperatures in the range of 42 to 55°C• Supplied with the recombinant RiboLock RNase Inhibitor• Complete kit oligo dT 18 and random hexamer primers included with the kitApplications• First strand cDNA synthesis for RT PCR and real time RT PCR• Construction of full length cDNA libraries• Antisense RNA synthesis Additional FeaturesThe recombinant RiboLock RNase Inhibitor supplied with the kit effectively protects RNA template from degradation It is fully compatible with the reverse transcription reaction as it maintains activity at temperatures up to 55°C The kit is supplied with both oligo dT 18 and random hexamer primers The oligo dT 18 primer anneals selectively to the poly A tail of mRNA Random hexamer primers do not require the presence of poly A therefore they can be used for transcription of the 5 end regions of mRNA or for cDNA synthesis using RNA without the poly A tail e g microRNAs Gene specific primers may also be used with the kit The first strand cDNA can be directly used as a template in PCR realtime PCR or in second strand cDNA synthesis
    Catalog Number:
    k1631
    Price:
    None
    Applications:
    Cloning|cDNA Libraries & Library Construction
    Category:
    Kits and Assays
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    Structured Review

    Thermo Fisher revertaid h minus m mulv reverse transcriptase first strand cdna synthesis kit
    Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates The kit includes RevertAid H Minus Reverse Transcriptase which has a point mutation that completely eliminates RNase H activity Therefore it does not degrade RNA in RNA DNA hybrids during synthesis of the first strand cDNA Highlights• High yields of full length first strand cDNA up to 13 kb• Increased reaction temperatures in the range of 42 to 55°C• Supplied with the recombinant RiboLock RNase Inhibitor• Complete kit oligo dT 18 and random hexamer primers included with the kitApplications• First strand cDNA synthesis for RT PCR and real time RT PCR• Construction of full length cDNA libraries• Antisense RNA synthesis Additional FeaturesThe recombinant RiboLock RNase Inhibitor supplied with the kit effectively protects RNA template from degradation It is fully compatible with the reverse transcription reaction as it maintains activity at temperatures up to 55°C The kit is supplied with both oligo dT 18 and random hexamer primers The oligo dT 18 primer anneals selectively to the poly A tail of mRNA Random hexamer primers do not require the presence of poly A therefore they can be used for transcription of the 5 end regions of mRNA or for cDNA synthesis using RNA without the poly A tail e g microRNAs Gene specific primers may also be used with the kit The first strand cDNA can be directly used as a template in PCR realtime PCR or in second strand cDNA synthesis
    https://www.bioz.com/result/revertaid h minus m mulv reverse transcriptase first strand cdna synthesis kit/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    revertaid h minus m mulv reverse transcriptase first strand cdna synthesis kit - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    RNA Extraction:

    Article Title: Comparative study of the aldehyde dehydrogenase (ALDH) gene superfamily in the glycophyte Arabidopsis thaliana and Eutrema halophytes
    Article Snippet: .. Total RNA extraction was performed according to For RT–PCR analyses, 2 μg of total RNAs were treated with 10 U of RNase-free DNase I (Roche, Mannheim, Germany) in a 10 μL reaction containing 1 × DNase I buffer (20 m m Tris–HCl pH 8·4, 50 m m KCl and 2 m,M MgCl2 ) at 37 °C for 30 min. Then, 1 μL of 25 m m EDTA was added and the reaction was heated at 65 °C for 15 min to deactivate the DNase I. First-strand cDNA synthesis was performed using the RevertAid™ H Minus First Strand cDNA Synthesis Kit (Fermentas, Burlington, Canada) following the protocol provided by the supplier. .. Here, we identified 16 ALDH genes from E. parvulum using the available genome sequence ( and ).

    Synthesized:

    Article Title: Pumilio-2 regulates translation of Nav1.6 to mediate homeostasis of membrane excitability
    Article Snippet: .. To isolate total RNA, the pellet was treated with TRIzol (Invitrogen, Paisley, UK) following the manufacturer’s instructions and cDNA synthesized using the Revert Aid H Minus first strand cDNA synthesis kit (Fermentas Life Sciences, Canada). .. The synthesized cDNA was amplified in a two-stage PCR amplification previously described.

    Purification:

    Article Title: Activity of NaV1.2 promotes neurodegeneration in an animal model of multiple sclerosis
    Article Snippet: .. mRNA was purified using the RNeasy Mini Kit (Qiagen) and reverse transcribed to cDNA with the RevertAid H Minus First-Strand cDNA Synthesis Kit (Fermentas) according to the manufacturer’s instructions. .. For quantitative real-time PCR, the following TaqMan Gene Expression Assays were used: Scn2a , Mm01270359_m1 and Tbp , Mm00446971_m1.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Comparative study of the aldehyde dehydrogenase (ALDH) gene superfamily in the glycophyte Arabidopsis thaliana and Eutrema halophytes
    Article Snippet: .. Total RNA extraction was performed according to For RT–PCR analyses, 2 μg of total RNAs were treated with 10 U of RNase-free DNase I (Roche, Mannheim, Germany) in a 10 μL reaction containing 1 × DNase I buffer (20 m m Tris–HCl pH 8·4, 50 m m KCl and 2 m,M MgCl2 ) at 37 °C for 30 min. Then, 1 μL of 25 m m EDTA was added and the reaction was heated at 65 °C for 15 min to deactivate the DNase I. First-strand cDNA synthesis was performed using the RevertAid™ H Minus First Strand cDNA Synthesis Kit (Fermentas, Burlington, Canada) following the protocol provided by the supplier. .. Here, we identified 16 ALDH genes from E. parvulum using the available genome sequence ( and ).

    Random Hexamer Labeling:

    Article Title: Comparative analysis of the repertoire of G protein-coupled receptors of three species of the fungal genus Trichoderma
    Article Snippet: .. For cDNA synthesis the Revert Aid H Minus First Strand cDNA Synthesis Kit (Fermentas, Vilnius, Lithuania) was used according to the manufacturer´s instructions with a combination of an oligo(dT)18 and a random hexamer primer. .. The sequences for the respective primer pairs for cDNA amplification of the reference gene sar1 and the genes encoding the putative receptors of class VIII identified in the Trichoderma genomes are given in Additional file .

    Polymerase Chain Reaction:

    Article Title: Klebsiella pneumoniae yggG Gene Product: A Zinc-Dependent Metalloprotease
    Article Snippet: .. Reverse Transcription PCR of yggG Gene RevertAid H Minus first strand cDNA synthesis kit (Fermentas) was used to synthesize the cDNA from the extracted total RNA. .. One microgram of total RNA was mixed with 0.2 μg random hexamer primer and DEPC-treated water.

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    Thermo Fisher revertaid h minus m mulv reverse transcriptase first strand cdna synthesis kit
    Revertaid H Minus M Mulv Reverse Transcriptase First Strand Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/revertaid h minus m mulv reverse transcriptase first strand cdna synthesis kit/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    revertaid h minus m mulv reverse transcriptase first strand cdna synthesis kit - by Bioz Stars, 2020-08
    99/100 stars
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