revert aid m mulv reverse transcriptase kit  (Thermo Fisher)


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    Name:
    RevertAid Reverse Transcriptase 200 U µL
    Description:
    Thermo Scientific RevertAid Reverse Transcriptase RT is a recombinant M MuLV RT It differs from the M MuLV RT by its structure and catalytic properties The enzyme possesses an RNA dependent and DNA dependent polymerase activity and a RNase H activity specific to RNA in RNA DNA hybrids which is significantly lower than that of Avian Myeloblastosis Virus AMV reverse transcriptase Highlights• Efficient synthesis of full length first strand cDNA up to 13 kb• Optimum activity at 42°C• Active up to 50°C• Incorporates modified nucleotides e g Cy3 Cy5 rhodamine aminoallyl fluorescein labeled nucleotides Applications• First strand cDNA synthesis for RT PCR and real time RT PCR• Synthesis of cDNA for cloning and expression• Generation of labeled cDNA probes for microarrays• DNA labeling• Analysis of RNA by primer extension
    Catalog Number:
    ep0441
    Price:
    None
    Applications:
    Cloning|PCR & Real-Time PCR|RT-PCR|Reverse Transcription|Two-Step RT-PCR|cDNA Libraries & Library Construction
    Category:
    Proteins Enzymes Peptides
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    Structured Review

    Thermo Fisher revert aid m mulv reverse transcriptase kit
    Thermo Scientific RevertAid Reverse Transcriptase RT is a recombinant M MuLV RT It differs from the M MuLV RT by its structure and catalytic properties The enzyme possesses an RNA dependent and DNA dependent polymerase activity and a RNase H activity specific to RNA in RNA DNA hybrids which is significantly lower than that of Avian Myeloblastosis Virus AMV reverse transcriptase Highlights• Efficient synthesis of full length first strand cDNA up to 13 kb• Optimum activity at 42°C• Active up to 50°C• Incorporates modified nucleotides e g Cy3 Cy5 rhodamine aminoallyl fluorescein labeled nucleotides Applications• First strand cDNA synthesis for RT PCR and real time RT PCR• Synthesis of cDNA for cloning and expression• Generation of labeled cDNA probes for microarrays• DNA labeling• Analysis of RNA by primer extension
    https://www.bioz.com/result/revert aid m mulv reverse transcriptase kit/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    revert aid m mulv reverse transcriptase kit - by Bioz Stars, 2020-07
    99/100 stars

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    Random Hexamer Labeling:

    Article Title: Arsenic abrogates the estrogen-signaling pathway in the rat uterus
    Article Snippet: .. Reverse transcription reaction was performed at 42°C with 5 μg of RNA in 5× reaction buffer (Fermentas, USA) containing 100 pmol random hexamer primer, 10 mM dNTP mixture (Fermentas, USA), 20 units of RNase inhibitor (Bioline, USA) and 200 units of RevertAid™ M-MuLV Reverse Transcriptase (Fermentas, USA). .. PCR was initiated using 2.5 μg cDNA, 10 mM dNTPs and 1 unit of Taq DNA Polymerase (Vivantis, USA).

    Article Title: Genetic recombination and diversity of sapovirus in pediatric patients with acute gastroenteritis in Thailand, 2010–2018
    Article Snippet: .. The viral RNA was reverse transcribed to cDNA using random hexamer primers and RevertAid™ reverse transcriptase ( https://www.thermofisher.com/ , USA) according to the manufacturer’s instruction. .. SaV was screened by conventional PCR method using GoTaq DNA polymerase (Promega, USA) with primers SLV5731 and SLV5749 ( ) targeting capsid region (VP1 gene) as described previously ( ).

    other:

    Article Title: Genomic Approach to Study Floral Development Genes in Rosa sp.
    Article Snippet: One microgram total RNA (treated with DNAse) was used in a reverse transcription assay with RevertAid M-MuLV Reverse Transcriptase (Fermentas, Burlington, Ontario).

    Article Title: Genome-wide analysis of lectin receptor-like kinases in Populus
    Article Snippet: One μg of total RNA was used to synthesize complementary DNA (cDNA) by reverse transcription with Revertaid reverse transcriptase (Thermo Fisher Scientific, Waltham, MA).

    Article Title: Developmental expression and function of DKKL1/Dkkl1 in humans and mice
    Article Snippet: Total RNA (2 μg) was reverse-transcribed into cDNA in a reaction primed by an oligodeoxynucleotide (dT)18 primer using RevertAidTM M-Mulv Reverse Transcriptase (Fermentas, Glen Burnie, MD, USA) according to the manufacturer’s instructions.

    Article Title: REBELOTE, SQUINT, and ULTRAPETALA1 Function Redundantly in the Temporal Regulation of Floral Meristem Termination in Arabidopsis thaliana [W]
    Article Snippet: The first-strand cDNA synthesis was performed on 0.6 μg of total RNA using RevertAid M-MuLV reverse transcriptase (Fermentas) and an oligo(dT) primer (18-mer) according to the manufacturer's instructions.

    Article Title: Human-specific epigenetic variation in the immunological Leukotriene B4 Receptor (LTB4R/BLT1) implicated in common inflammatory diseases
    Article Snippet: Reverse transcription of RNA was carried out using RevertAid M-MuLV reverse transcriptase (Fermentas, Thermo Scientific, Waltham, MA, USA) and primed using random hexamers according to the manufacturer’s protocol.

    Article Title: Drought Tolerance Is Correlated with the Activity of Antioxidant Enzymes in Cerasus humilis Seedlings
    Article Snippet: One-microgram total RNA was performed in reverse transcription with RevertAid Reverse Transcriptase (Fermentas) and Oligo d(T)primers (TaKaRa).

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    https://www.bioz.com/result/revert aid m mulv reverse transkriptase kit/product/Thermo Fisher
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    revert aid m mulv reverse transkriptase kit - by Bioz Stars, 2020-07
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    99
    Thermo Fisher revert aid m mulv reverse transcriptase kit
    Revert Aid M Mulv Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/revert aid m mulv reverse transcriptase kit/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    revert aid m mulv reverse transcriptase kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

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