revert aid m mulv reverse transcriptase kit  (Thermo Fisher)

 
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    Name:
    RevertAid Reverse Transcriptase 200 U µL
    Description:
    Thermo Scientific RevertAid Reverse Transcriptase RT is a recombinant M MuLV RT It differs from the M MuLV RT by its structure and catalytic properties The enzyme possesses an RNA dependent and DNA dependent polymerase activity and a RNase H activity specific to RNA in RNA DNA hybrids which is significantly lower than that of Avian Myeloblastosis Virus AMV reverse transcriptase Highlights• Efficient synthesis of full length first strand cDNA up to 13 kb• Optimum activity at 42°C• Active up to 50°C• Incorporates modified nucleotides e g Cy3 Cy5 rhodamine aminoallyl fluorescein labeled nucleotides Applications• First strand cDNA synthesis for RT PCR and real time RT PCR• Synthesis of cDNA for cloning and expression• Generation of labeled cDNA probes for microarrays• DNA labeling• Analysis of RNA by primer extension
    Catalog Number:
    ep0441
    Price:
    None
    Applications:
    Cloning|PCR & Real-Time PCR|RT-PCR|Reverse Transcription|Two-Step RT-PCR|cDNA Libraries & Library Construction
    Category:
    Proteins Enzymes Peptides
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    Structured Review

    Thermo Fisher revert aid m mulv reverse transcriptase kit
    Thermo Scientific RevertAid Reverse Transcriptase RT is a recombinant M MuLV RT It differs from the M MuLV RT by its structure and catalytic properties The enzyme possesses an RNA dependent and DNA dependent polymerase activity and a RNase H activity specific to RNA in RNA DNA hybrids which is significantly lower than that of Avian Myeloblastosis Virus AMV reverse transcriptase Highlights• Efficient synthesis of full length first strand cDNA up to 13 kb• Optimum activity at 42°C• Active up to 50°C• Incorporates modified nucleotides e g Cy3 Cy5 rhodamine aminoallyl fluorescein labeled nucleotides Applications• First strand cDNA synthesis for RT PCR and real time RT PCR• Synthesis of cDNA for cloning and expression• Generation of labeled cDNA probes for microarrays• DNA labeling• Analysis of RNA by primer extension
    https://www.bioz.com/result/revert aid m mulv reverse transcriptase kit/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    revert aid m mulv reverse transcriptase kit - by Bioz Stars, 2021-02
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: The flavoprotein Mcap0476 (RlmFO) catalyzes m5U1939 modification in Mycoplasma capricolum 23S rRNA
    Article Snippet: .. Total RNA was extracted from positives clones (Tri Reagent, Sigma) and Mcap0476 transcripts were detected by RT-PCR analysis (ThermoScript Reverse Transcriptase, Invitrogen) using the primer pair TrmFO1adir-3 (5′-GTTATATAGTTAGTTAGTG) and TrmFO1arev-3 (5′-GTTAGTTATATTGATGATG) giving a Mcap0476 cDNA fragment of 788 bp. ..

    Quantitative RT-PCR:

    Article Title: High-Throughput Functional MicroRNAs Profiling by Recombinant AAV-Based MicroRNA Sensor Arrays
    Article Snippet: .. To confirm our results, we tested the expression level of miR-21 by quantitative reverse transcriptase (QRT)-PCR (Ambion) ( ). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Activation-dependent stabilization of the human thromboxane receptor: role of reactive oxygen species
    Article Snippet: .. Total RNA was extracted (RNeasy™; Qiagen, CA), reverse transcribed into cDNA (TaqMan™ reverse transcriptase; Applied Biosystems, CA), and TP mRNA quantified by real-time PCR analysis. .. Primers and probes were from Ambion, CA (Hs00169054_m1). mRNA levels were normalized by subtracting the Ct for β-actin or 18S from the Ct for TP, producing a ΔCt value.

    Polymerase Chain Reaction:

    Article Title: Conditional targeting of phosphatidylserine decarboxylase to lipid droplets
    Article Snippet: .. RNA was used as a template for reverse-transcriptase polymerase chain reaction (RT-PCR) using the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, USA). .. The pPISD-LD-eGFP vector was used as substrate to generate different mutants of PISD-LD by Q5® Site-Directed Mutagenesis kit (NEB).

    Article Title: Temtamy Preaxial Brachydactyly Syndrome Is Caused by Loss-of-Function Mutations in Chondroitin Synthase 1, a Potential Target of BMP Signaling
    Article Snippet: .. Reverse-transcriptase polymerase chain reaction (RT-PCR) was performed with RevertAid First Strand cDNA synthesis Kit (Fermentas). ..

    Expressing:

    Article Title: High-Throughput Functional MicroRNAs Profiling by Recombinant AAV-Based MicroRNA Sensor Arrays
    Article Snippet: .. To confirm our results, we tested the expression level of miR-21 by quantitative reverse transcriptase (QRT)-PCR (Ambion) ( ). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Conditional targeting of phosphatidylserine decarboxylase to lipid droplets
    Article Snippet: .. RNA was used as a template for reverse-transcriptase polymerase chain reaction (RT-PCR) using the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, USA). .. The pPISD-LD-eGFP vector was used as substrate to generate different mutants of PISD-LD by Q5® Site-Directed Mutagenesis kit (NEB).

    Article Title: Temtamy Preaxial Brachydactyly Syndrome Is Caused by Loss-of-Function Mutations in Chondroitin Synthase 1, a Potential Target of BMP Signaling
    Article Snippet: .. Reverse-transcriptase polymerase chain reaction (RT-PCR) was performed with RevertAid First Strand cDNA synthesis Kit (Fermentas). ..

    Article Title: The flavoprotein Mcap0476 (RlmFO) catalyzes m5U1939 modification in Mycoplasma capricolum 23S rRNA
    Article Snippet: .. Total RNA was extracted from positives clones (Tri Reagent, Sigma) and Mcap0476 transcripts were detected by RT-PCR analysis (ThermoScript Reverse Transcriptase, Invitrogen) using the primer pair TrmFO1adir-3 (5′-GTTATATAGTTAGTTAGTG) and TrmFO1arev-3 (5′-GTTAGTTATATTGATGATG) giving a Mcap0476 cDNA fragment of 788 bp. ..

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  • 97
    Thermo Fisher revert aid m mulv reverse transkriptase kit
    Revert Aid M Mulv Reverse Transkriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/revert aid m mulv reverse transkriptase kit/product/Thermo Fisher
    Average 97 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    revert aid m mulv reverse transkriptase kit - by Bioz Stars, 2021-02
    97/100 stars
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    Thermo Fisher revertaid first strand cdna synthesis kit
    Revertaid First Strand Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 2030 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/revertaid first strand cdna synthesis kit/product/Thermo Fisher
    Average 97 stars, based on 2030 article reviews
    Price from $9.99 to $1999.99
    revertaid first strand cdna synthesis kit - by Bioz Stars, 2021-02
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    99
    Thermo Fisher revert aid m mulv reverse transcriptase kit
    Revert Aid M Mulv Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/revert aid m mulv reverse transcriptase kit/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    revert aid m mulv reverse transcriptase kit - by Bioz Stars, 2021-02
    99/100 stars
      Buy from Supplier

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