reversed phase trap column  (Thermo Fisher)


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    Structured Review

    Thermo Fisher reversed phase trap column
    Reversed Phase Trap Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reversed phase trap column/product/Thermo Fisher
    Average 98 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    reversed phase trap column - by Bioz Stars, 2020-04
    98/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Comprehensive Annotation of the Parastagonospora nodorum Reference Genome Using Next-Generation Genomics, Transcriptomics and Proteogenomics
    Article Snippet: Tryptic peptides were separated on a Prominence nano HPLC system (Shimadzu, Kyoto, Japan) and data collected on a Hybrid LTQ Orbitrap mass spectrometer (Thermo Fisher Scientific, Bremen, Germany). .. Acidified Offgel fractions were loaded onto a reversed-phase trap column (Dionex Acclaim PepMap μ-Precolumn C18, 0.3 mm x 5 mm) at 30 μL/min in 100% eluent A for 3.5 minutes and subsequently separated on a reversed phase capillary column (Vydac Everest C18 5μm 300 Å, 150 μm x150 mm, Alltech) at 45°C and a flow rate of 1 μL/min.

    Article Title: Mutations in the pantothenate kinase of Plasmodium falciparum confer diverse sensitivity profiles to antiplasmodial pantothenate analogues
    Article Snippet: Briefly, samples were loaded at a flow rate of 15 μ L/min onto a reversed-phase trap column (100 μ m × 2 cm; Acclaim PepMap media, Dionex), which was maintained at a temperature of 40°C. .. The HPLC gradient was set to 158 min using a gradient that reached 30% ACN after 123 min, then 34% ACN after 126 min, 79.2% ACN after 131 min and 2% ACN after 138 min, at which it was maintained for a further 20 min.

    Article Title: Comparative Analysis of Erythrocyte Proteomes of Water Buffalo, Dairy Cattle, and Beef Cattle by Shotgun LC-MS/MS
    Article Snippet: .. HPLC-ESI-MS/MS (Shotgun Analysis) The peptide mixture (3 ug) was loaded onto a reversed-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 200 mm, nanoViper C18) connected to a C18 reversed-phase analytical column (Thermo Scientific Easy Column, 10 cm long, 75 μm inner diameter, 3 μm resin) in buffer A (0.1% Formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% Formic acid) at a flow rate of 300 nl/min controlled by IntelliFlow technology. .. The linear gradient was determined by the project proposal: 0–35% buffer B for 50 min, 35–100% buffer B for 5 min, then being held in 100% buffer B for 5 min. LC-MS/MS analysis was performed on a Q Exactive mass spectrometer (Thermo Scientific) that was coupled to an Easy nLC (Thermo Fisher Scientific) for 60 min.

    Article Title: Differential proteomic analysis of replanted Rehmannia glutinosa roots by iTRAQ reveals molecular mechanisms for formation of replant disease
    Article Snippet: .. Reverse-phase nanoflow HPLC and tandem mass spectrometry SCX fractions were dissolved in buffer A (0.1% formic acid) and loaded onto a reversed-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18). .. Peptides were separated with a linear gradient of buffer B (84% acetonitrile and 0.1% formic acid) at a flow rate of 300 nl/min.

    Article Title: Mutations in the pantothenate kinase of Plasmodium falciparum confer diverse sensitivity profiles to antiplasmodial pantothenate analogues
    Article Snippet: Briefly, samples were loaded at a flow rate of 15 μ L/min onto a reversed-phase trap column (100 μ m × 2 cm; Acclaim PepMap media, Dionex), which was maintained at a temperature of 40°C. .. The HPLC gradient was set to 158 min using a gradient that reached 30% ACN after 123 min, then 34% ACN after 126 min, 79.2% ACN after 131 min and 2% ACN after 138 min, at which it was maintained for a further 20 min.

    Flow Cytometry:

    Article Title: Comparative Transcriptomic and Proteomic Analyses Identify Key Genes Associated With Milk Fat Traits in Chinese Holstein Cows
    Article Snippet: .. The peptide mixture was loaded onto a reversed-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18) connected to a C18 reversed-phase analytical column (length = 10 cm, inner diameter = 75-μm, 3-μm resin; Thermo Fisher Scientific) in buffer A (0.1% formic acid) and separated for 1.5 h with a linear gradient of buffer B (98% acetonitrile and 0.1% formic acid) at a flow rate of 300 nL/min controlled by IntelliFlow technology (4%–7% buffer B for 2 min, 7%–20% buffer B for 65 min, 20%–35% buffer B for 12 min, 35%–90% buffer B for 2 min, and holding in 90% buffer B for 9 min). .. Liquid Chromatography Tandem MS (LC-MS/MS) Analysis LC-MS/MS analysis was performed on a Q-exactive Plus Orbitrap mass spectrometer (Thermo Fisher Scientific) coupled to an Easy nLC chromatograph (Proxeon Biosystems, now Thermo Fisher Scientific) for 90 min.

    Article Title: Comprehensive Annotation of the Parastagonospora nodorum Reference Genome Using Next-Generation Genomics, Transcriptomics and Proteogenomics
    Article Snippet: .. Acidified Offgel fractions were loaded onto a reversed-phase trap column (Dionex Acclaim PepMap μ-Precolumn C18, 0.3 mm x 5 mm) at 30 μL/min in 100% eluent A for 3.5 minutes and subsequently separated on a reversed phase capillary column (Vydac Everest C18 5μm 300 Å, 150 μm x150 mm, Alltech) at 45°C and a flow rate of 1 μL/min. ..

    Article Title: Neuropeptide Release Is Impaired in a Mouse Model of Fragile X Mental Retardation Syndrome
    Article Snippet: .. Briefly, the samples were initially loaded onto a 5-μL loop using a manual injector (VALCO Instruments Co. Inc., Houston, TX) and subsequently trapped onto a reversed-phase trap column (PepMap, C18, 5 μm, 100 Å, LC Packings, a Dionex Company, Sunnyvale, CA) using solvent C (90% water, 10% acetonitrile (ACN), 0.1% formic acid (FA), and 0.01% trifluoroacetic acid (TFA)) at a flow rate of 8 μL/min and washed for 5 min. Each sample was then eluted and separated on a reversed-phase column (LC Packings 300-μm i.d. .. × 15 cm, C18 PepMap100, 100 Å) using a gradient at 2 μL/min flow rate for 45 min. Gradients were generated using solvent A (95% water, 5% ACN, 0.1% TFA) and solvent B (95% ACN, 5% water, 0.1% FA, and 0.01% TFA).

    Article Title: SCD1 activation impedes foam cell formation by inducing lipophagy in oxLDL‐treated human vascular smooth muscle cells, et al. SCD1 activation impedes foam cell formation by inducing lipophagy in oxLDL‐treated human vascular smooth muscle cells
    Article Snippet: The peptide mixture was loaded onto a reversed‐phase trap column (Thermo Fisher) that was desalted with C18 spin tips. .. The flow rate was 300 nL/min (IntelliFlow) and the gradient was programmed as follows: buffer solution B (0%‐55%) for 80 minutes, buffer solution B (55%‐100%) for 5 minutes and buffer solution B (100%) for 5 minutes.

    Article Title: Mutations in the pantothenate kinase of Plasmodium falciparum confer diverse sensitivity profiles to antiplasmodial pantothenate analogues
    Article Snippet: .. Briefly, samples were loaded at a flow rate of 15 μ L/min onto a reversed-phase trap column (100 μ m × 2 cm; Acclaim PepMap media, Dionex), which was maintained at a temperature of 40°C. .. Peptides were then eluted from the trap column at a flow rate of 0.25 μ L/min through a reversed-phase capillary column (75 μ m × 50 cm; LC Packings, Dionex).

    Article Title: Quantitative proteomics analysis to identify biomarkers of chronic myofascial pain and therapeutic targets of dry needling in a rat model of myofascial trigger points
    Article Snippet: The fractionated peptide mixture was placed onto a reversed-phase trap column, 100 µm×2 cm (nano Viper C18) (Thermo Fisher Scientific, Waltham, MA, USA) coupled with a C18 reversed-phase analytical column with an inner diameter of 75 µm, a length of 10 cm, and 3 µm of resin (Thermo Fisher Scientific) in 0.1% formic acid buffer. .. The flow rate was controlled at 300 mL/min using IntelliFlow Technology (Thermo Fisher Scientific).

    Article Title: iTRAQ Proteomic Analysis of Continuously Cropped Soybean Root Inoculated With Funneliformis mosseae
    Article Snippet: .. LC-MS/MS Analysis For nanoLC-MS/MS analysis, each peptide mixture was injected onto a reversed-phase trap column (Thermo Scientific Acclaim PepMap100; 100 μm × 2 cm, nanoViper C18) connected to a C18 reversed-phase analytical column (Thermo Scientific Easy Column; 10 cm long, 75 μm inner diameter, 3 μm resin) equilibrated in buffer A (0.1% formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% formic acid) at a flow rate of 300 nl/min controlled using IntelliFlow technology. ..

    Article Title: Comparative Analysis of Erythrocyte Proteomes of Water Buffalo, Dairy Cattle, and Beef Cattle by Shotgun LC-MS/MS
    Article Snippet: .. HPLC-ESI-MS/MS (Shotgun Analysis) The peptide mixture (3 ug) was loaded onto a reversed-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 200 mm, nanoViper C18) connected to a C18 reversed-phase analytical column (Thermo Scientific Easy Column, 10 cm long, 75 μm inner diameter, 3 μm resin) in buffer A (0.1% Formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% Formic acid) at a flow rate of 300 nl/min controlled by IntelliFlow technology. .. The linear gradient was determined by the project proposal: 0–35% buffer B for 50 min, 35–100% buffer B for 5 min, then being held in 100% buffer B for 5 min. LC-MS/MS analysis was performed on a Q Exactive mass spectrometer (Thermo Scientific) that was coupled to an Easy nLC (Thermo Fisher Scientific) for 60 min.

    Article Title: Differential proteomic analysis of replanted Rehmannia glutinosa roots by iTRAQ reveals molecular mechanisms for formation of replant disease
    Article Snippet: Reverse-phase nanoflow HPLC and tandem mass spectrometry SCX fractions were dissolved in buffer A (0.1% formic acid) and loaded onto a reversed-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18). .. Peptides were separated with a linear gradient of buffer B (84% acetonitrile and 0.1% formic acid) at a flow rate of 300 nl/min.

    Article Title: Transcriptomic and proteomic analysis reveals mechanisms of low pollen-pistil compatibility during water lily cross breeding
    Article Snippet: .. We first loaded the peptide mixture onto reversed-phase trap column (Thermo Fisher Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18) and connected it to C18-reversed phase analytical column (Thermo Fisher Scientific Easy Column, 75 μm inner diameter, 10 cm long, 3 μm, C18-A2) in buffer A (0.1% formic acid), and then separated it with the linear gradient of buffer B (0.1% formic acid, 84% acetonitrile) under the flow rate of 300 nL / min controlled by intelliFlow technology. .. The LC-MS/MS analysis was carried out on the Q Exactive mass spectrometer (Thermo Fisher Scientific) coupled with a Easy nLC (Proxeon Biosystems, now Thermo Fisher Scientific) for 240 min. Mass spectrometer was operated under positive ion mode.

    Article Title: Mutations in the pantothenate kinase of Plasmodium falciparum confer diverse sensitivity profiles to antiplasmodial pantothenate analogues
    Article Snippet: .. Briefly, samples were loaded at a flow rate of 15 μ L/min onto a reversed-phase trap column (100 μ m × 2 cm; Acclaim PepMap media, Dionex), which was maintained at a temperature of 40°C. .. Peptides were then eluted from the trap column at a flow rate of 0.25 μ L/min through a reversed-phase capillary column (75 μ m × 50 cm; LC Packings, Dionex).

    Selection:

    Article Title: Comprehensive Annotation of the Parastagonospora nodorum Reference Genome Using Next-Generation Genomics, Transcriptomics and Proteogenomics
    Article Snippet: Acidified Offgel fractions were loaded onto a reversed-phase trap column (Dionex Acclaim PepMap μ-Precolumn C18, 0.3 mm x 5 mm) at 30 μL/min in 100% eluent A for 3.5 minutes and subsequently separated on a reversed phase capillary column (Vydac Everest C18 5μm 300 Å, 150 μm x150 mm, Alltech) at 45°C and a flow rate of 1 μL/min. .. Data was either acquired on an LTQ Orbitrap Velos as outlined below or an LTQ Orbitrap XL as described in Hastie et al. [ ] with the following modifications to data acquisition: target value of 1 x 103 for ion trap MS/MS scans; dynamic exclusion set to 70 s; ion selection threshold 1000 counts.

    Chromatography:

    Article Title: Quantitative proteomics analysis to identify biomarkers of chronic myofascial pain and therapeutic targets of dry needling in a rat model of myofascial trigger points
    Article Snippet: After TMT labeling, liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was performed. .. The fractionated peptide mixture was placed onto a reversed-phase trap column, 100 µm×2 cm (nano Viper C18) (Thermo Fisher Scientific, Waltham, MA, USA) coupled with a C18 reversed-phase analytical column with an inner diameter of 75 µm, a length of 10 cm, and 3 µm of resin (Thermo Fisher Scientific) in 0.1% formic acid buffer.

    Article Title: iTRAQ Proteomic Analysis of Continuously Cropped Soybean Root Inoculated With Funneliformis mosseae
    Article Snippet: LC-MS/MS Analysis For nanoLC-MS/MS analysis, each peptide mixture was injected onto a reversed-phase trap column (Thermo Scientific Acclaim PepMap100; 100 μm × 2 cm, nanoViper C18) connected to a C18 reversed-phase analytical column (Thermo Scientific Easy Column; 10 cm long, 75 μm inner diameter, 3 μm resin) equilibrated in buffer A (0.1% formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% formic acid) at a flow rate of 300 nl/min controlled using IntelliFlow technology. .. Liquid chromatography-tandem mass spectrometry analysis was performed on a Q Exactive mass spectrometer (Thermo Scientific) coupled to an Easy nLC (Proxeon Biosystems, now Thermo Fisher Scientific) over a 60 min period in positive ion mode.

    Isolation:

    Article Title: Comparative Transcriptomic and Proteomic Analyses Identify Key Genes Associated With Milk Fat Traits in Chinese Holstein Cows
    Article Snippet: Paragraph title: Protein Isolation, Enzymolysis, and TMT Labeling ... The peptide mixture was loaded onto a reversed-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18) connected to a C18 reversed-phase analytical column (length = 10 cm, inner diameter = 75-μm, 3-μm resin; Thermo Fisher Scientific) in buffer A (0.1% formic acid) and separated for 1.5 h with a linear gradient of buffer B (98% acetonitrile and 0.1% formic acid) at a flow rate of 300 nL/min controlled by IntelliFlow technology (4%–7% buffer B for 2 min, 7%–20% buffer B for 65 min, 20%–35% buffer B for 12 min, 35%–90% buffer B for 2 min, and holding in 90% buffer B for 9 min).

    Article Title: Mutations in the pantothenate kinase of Plasmodium falciparum confer diverse sensitivity profiles to antiplasmodial pantothenate analogues
    Article Snippet: Briefly, samples were loaded at a flow rate of 15 μ L/min onto a reversed-phase trap column (100 μ m × 2 cm; Acclaim PepMap media, Dionex), which was maintained at a temperature of 40°C. .. The mass spectrometer was operated in a data-independent acquisition (DIA) mode with a 25-fixed-window setup of 24 m/z effective precursor isolation over the m/z range of 375–975 Da.

    Article Title: Differential proteomic analysis of replanted Rehmannia glutinosa roots by iTRAQ reveals molecular mechanisms for formation of replant disease
    Article Snippet: Reverse-phase nanoflow HPLC and tandem mass spectrometry SCX fractions were dissolved in buffer A (0.1% formic acid) and loaded onto a reversed-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18). .. Survey scans were acquired at a resolution of 70,000 at 200 m /z ; resolution for HCD spectra was set to 17,500 at 200 m /z , and isolation width was 2 m /z .

    Article Title: Mutations in the pantothenate kinase of Plasmodium falciparum confer diverse sensitivity profiles to antiplasmodial pantothenate analogues
    Article Snippet: Briefly, samples were loaded at a flow rate of 15 μ L/min onto a reversed-phase trap column (100 μ m × 2 cm; Acclaim PepMap media, Dionex), which was maintained at a temperature of 40°C. .. The mass spectrometer was operated in a data-independent acquisition (DIA) mode with a 25-fixed-window setup of 24 m/z effective precursor isolation over the m/z range of 375–975 Da.

    BIA-KA:

    Article Title: Comparative Transcriptomic and Proteomic Analyses Identify Key Genes Associated With Milk Fat Traits in Chinese Holstein Cows
    Article Snippet: The homogenate was boiled for 3 min and then sonicated for 2 min. After centrifugation at 20,000 × g for 20 min at 4°C, the concentration of proteins in the filtrate was quantified with a BCA Protein Assay Kit (Bio-Rad, Hercules, CA, USA). .. The peptide mixture was loaded onto a reversed-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18) connected to a C18 reversed-phase analytical column (length = 10 cm, inner diameter = 75-μm, 3-μm resin; Thermo Fisher Scientific) in buffer A (0.1% formic acid) and separated for 1.5 h with a linear gradient of buffer B (98% acetonitrile and 0.1% formic acid) at a flow rate of 300 nL/min controlled by IntelliFlow technology (4%–7% buffer B for 2 min, 7%–20% buffer B for 65 min, 20%–35% buffer B for 12 min, 35%–90% buffer B for 2 min, and holding in 90% buffer B for 9 min).

    Article Title: Mutations in the pantothenate kinase of Plasmodium falciparum confer diverse sensitivity profiles to antiplasmodial pantothenate analogues
    Article Snippet: For sample processing, a total of 500 μ g of protein, accurately determined using the Pierce BCA protein assay kit (ThermoFisher), was incubated overnight with sequencing-grade trypsin (1:50 dilution; Promega). .. Briefly, samples were loaded at a flow rate of 15 μ L/min onto a reversed-phase trap column (100 μ m × 2 cm; Acclaim PepMap media, Dionex), which was maintained at a temperature of 40°C.

    Article Title: Mutations in the pantothenate kinase of Plasmodium falciparum confer diverse sensitivity profiles to antiplasmodial pantothenate analogues
    Article Snippet: For sample processing, a total of 500 μ g of protein, accurately determined using the Pierce BCA protein assay kit (ThermoFisher), was incubated overnight with sequencing-grade trypsin (1:50 dilution; Promega). .. Briefly, samples were loaded at a flow rate of 15 μ L/min onto a reversed-phase trap column (100 μ m × 2 cm; Acclaim PepMap media, Dionex), which was maintained at a temperature of 40°C.

    Centrifugation:

    Article Title: Comparative Transcriptomic and Proteomic Analyses Identify Key Genes Associated With Milk Fat Traits in Chinese Holstein Cows
    Article Snippet: The homogenate was boiled for 3 min and then sonicated for 2 min. After centrifugation at 20,000 × g for 20 min at 4°C, the concentration of proteins in the filtrate was quantified with a BCA Protein Assay Kit (Bio-Rad, Hercules, CA, USA). .. The peptide mixture was loaded onto a reversed-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18) connected to a C18 reversed-phase analytical column (length = 10 cm, inner diameter = 75-μm, 3-μm resin; Thermo Fisher Scientific) in buffer A (0.1% formic acid) and separated for 1.5 h with a linear gradient of buffer B (98% acetonitrile and 0.1% formic acid) at a flow rate of 300 nL/min controlled by IntelliFlow technology (4%–7% buffer B for 2 min, 7%–20% buffer B for 65 min, 20%–35% buffer B for 12 min, 35%–90% buffer B for 2 min, and holding in 90% buffer B for 9 min).

    Labeling:

    Article Title: Comparative Transcriptomic and Proteomic Analyses Identify Key Genes Associated With Milk Fat Traits in Chinese Holstein Cows
    Article Snippet: Paragraph title: Protein Isolation, Enzymolysis, and TMT Labeling ... The peptide mixture was loaded onto a reversed-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18) connected to a C18 reversed-phase analytical column (length = 10 cm, inner diameter = 75-μm, 3-μm resin; Thermo Fisher Scientific) in buffer A (0.1% formic acid) and separated for 1.5 h with a linear gradient of buffer B (98% acetonitrile and 0.1% formic acid) at a flow rate of 300 nL/min controlled by IntelliFlow technology (4%–7% buffer B for 2 min, 7%–20% buffer B for 65 min, 20%–35% buffer B for 12 min, 35%–90% buffer B for 2 min, and holding in 90% buffer B for 9 min).

    Article Title: Quantitative proteomics analysis to identify biomarkers of chronic myofascial pain and therapeutic targets of dry needling in a rat model of myofascial trigger points
    Article Snippet: After TMT labeling, liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was performed. .. The fractionated peptide mixture was placed onto a reversed-phase trap column, 100 µm×2 cm (nano Viper C18) (Thermo Fisher Scientific, Waltham, MA, USA) coupled with a C18 reversed-phase analytical column with an inner diameter of 75 µm, a length of 10 cm, and 3 µm of resin (Thermo Fisher Scientific) in 0.1% formic acid buffer.

    Concentration Assay:

    Article Title: Comparative Transcriptomic and Proteomic Analyses Identify Key Genes Associated With Milk Fat Traits in Chinese Holstein Cows
    Article Snippet: The homogenate was boiled for 3 min and then sonicated for 2 min. After centrifugation at 20,000 × g for 20 min at 4°C, the concentration of proteins in the filtrate was quantified with a BCA Protein Assay Kit (Bio-Rad, Hercules, CA, USA). .. The peptide mixture was loaded onto a reversed-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18) connected to a C18 reversed-phase analytical column (length = 10 cm, inner diameter = 75-μm, 3-μm resin; Thermo Fisher Scientific) in buffer A (0.1% formic acid) and separated for 1.5 h with a linear gradient of buffer B (98% acetonitrile and 0.1% formic acid) at a flow rate of 300 nL/min controlled by IntelliFlow technology (4%–7% buffer B for 2 min, 7%–20% buffer B for 65 min, 20%–35% buffer B for 12 min, 35%–90% buffer B for 2 min, and holding in 90% buffer B for 9 min).

    Incubation:

    Article Title: Comprehensive Annotation of the Parastagonospora nodorum Reference Genome Using Next-Generation Genomics, Transcriptomics and Proteogenomics
    Article Snippet: Samples were incubated at 37°C for 2 hours followed by the addition of further 1 μg of modified trypsin and 6 hours (Offgel fractions) and 14 hours (CWM proteome) digestion at 37°C. .. Acidified Offgel fractions were loaded onto a reversed-phase trap column (Dionex Acclaim PepMap μ-Precolumn C18, 0.3 mm x 5 mm) at 30 μL/min in 100% eluent A for 3.5 minutes and subsequently separated on a reversed phase capillary column (Vydac Everest C18 5μm 300 Å, 150 μm x150 mm, Alltech) at 45°C and a flow rate of 1 μL/min.

    Article Title: Mutations in the pantothenate kinase of Plasmodium falciparum confer diverse sensitivity profiles to antiplasmodial pantothenate analogues
    Article Snippet: For sample processing, a total of 500 μ g of protein, accurately determined using the Pierce BCA protein assay kit (ThermoFisher), was incubated overnight with sequencing-grade trypsin (1:50 dilution; Promega). .. Briefly, samples were loaded at a flow rate of 15 μ L/min onto a reversed-phase trap column (100 μ m × 2 cm; Acclaim PepMap media, Dionex), which was maintained at a temperature of 40°C.

    Article Title: Mutations in the pantothenate kinase of Plasmodium falciparum confer diverse sensitivity profiles to antiplasmodial pantothenate analogues
    Article Snippet: For sample processing, a total of 500 μ g of protein, accurately determined using the Pierce BCA protein assay kit (ThermoFisher), was incubated overnight with sequencing-grade trypsin (1:50 dilution; Promega). .. Briefly, samples were loaded at a flow rate of 15 μ L/min onto a reversed-phase trap column (100 μ m × 2 cm; Acclaim PepMap media, Dionex), which was maintained at a temperature of 40°C.

    Fractionation:

    Article Title: SCD1 activation impedes foam cell formation by inducing lipophagy in oxLDL‐treated human vascular smooth muscle cells, et al. SCD1 activation impedes foam cell formation by inducing lipophagy in oxLDL‐treated human vascular smooth muscle cells
    Article Snippet: Lastly, the labelled peptides were divided into 10 fractions and then fractionated by using a high pH reversed‐phase fractionation kit. .. The peptide mixture was loaded onto a reversed‐phase trap column (Thermo Fisher) that was desalted with C18 spin tips.

    Mass Spectrometry:

    Article Title: Comprehensive Annotation of the Parastagonospora nodorum Reference Genome Using Next-Generation Genomics, Transcriptomics and Proteogenomics
    Article Snippet: Tryptic peptides were separated on a Prominence nano HPLC system (Shimadzu, Kyoto, Japan) and data collected on a Hybrid LTQ Orbitrap mass spectrometer (Thermo Fisher Scientific, Bremen, Germany). .. Acidified Offgel fractions were loaded onto a reversed-phase trap column (Dionex Acclaim PepMap μ-Precolumn C18, 0.3 mm x 5 mm) at 30 μL/min in 100% eluent A for 3.5 minutes and subsequently separated on a reversed phase capillary column (Vydac Everest C18 5μm 300 Å, 150 μm x150 mm, Alltech) at 45°C and a flow rate of 1 μL/min.

    Article Title: SCD1 activation impedes foam cell formation by inducing lipophagy in oxLDL‐treated human vascular smooth muscle cells, et al. SCD1 activation impedes foam cell formation by inducing lipophagy in oxLDL‐treated human vascular smooth muscle cells
    Article Snippet: The peptide mixture was loaded onto a reversed‐phase trap column (Thermo Fisher) that was desalted with C18 spin tips. .. LC‐MS/MS analysis was carried out for 90 minutes with application of a Q Exactive mass spectrometer (Thermo Fisher).

    Article Title: Mutations in the pantothenate kinase of Plasmodium falciparum confer diverse sensitivity profiles to antiplasmodial pantothenate analogues
    Article Snippet: Briefly, samples were loaded at a flow rate of 15 μ L/min onto a reversed-phase trap column (100 μ m × 2 cm; Acclaim PepMap media, Dionex), which was maintained at a temperature of 40°C. .. The mass spectrometer was operated in a data-independent acquisition (DIA) mode with a 25-fixed-window setup of 24 m/z effective precursor isolation over the m/z range of 375–975 Da.

    Article Title: Quantitative proteomics analysis to identify biomarkers of chronic myofascial pain and therapeutic targets of dry needling in a rat model of myofascial trigger points
    Article Snippet: After TMT labeling, liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was performed. .. The fractionated peptide mixture was placed onto a reversed-phase trap column, 100 µm×2 cm (nano Viper C18) (Thermo Fisher Scientific, Waltham, MA, USA) coupled with a C18 reversed-phase analytical column with an inner diameter of 75 µm, a length of 10 cm, and 3 µm of resin (Thermo Fisher Scientific) in 0.1% formic acid buffer.

    Article Title: iTRAQ Proteomic Analysis of Continuously Cropped Soybean Root Inoculated With Funneliformis mosseae
    Article Snippet: LC-MS/MS Analysis For nanoLC-MS/MS analysis, each peptide mixture was injected onto a reversed-phase trap column (Thermo Scientific Acclaim PepMap100; 100 μm × 2 cm, nanoViper C18) connected to a C18 reversed-phase analytical column (Thermo Scientific Easy Column; 10 cm long, 75 μm inner diameter, 3 μm resin) equilibrated in buffer A (0.1% formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% formic acid) at a flow rate of 300 nl/min controlled using IntelliFlow technology. .. Liquid chromatography-tandem mass spectrometry analysis was performed on a Q Exactive mass spectrometer (Thermo Scientific) coupled to an Easy nLC (Proxeon Biosystems, now Thermo Fisher Scientific) over a 60 min period in positive ion mode.

    Article Title: Comparative Analysis of Erythrocyte Proteomes of Water Buffalo, Dairy Cattle, and Beef Cattle by Shotgun LC-MS/MS
    Article Snippet: HPLC-ESI-MS/MS (Shotgun Analysis) The peptide mixture (3 ug) was loaded onto a reversed-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 200 mm, nanoViper C18) connected to a C18 reversed-phase analytical column (Thermo Scientific Easy Column, 10 cm long, 75 μm inner diameter, 3 μm resin) in buffer A (0.1% Formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% Formic acid) at a flow rate of 300 nl/min controlled by IntelliFlow technology. .. The linear gradient was determined by the project proposal: 0–35% buffer B for 50 min, 35–100% buffer B for 5 min, then being held in 100% buffer B for 5 min. LC-MS/MS analysis was performed on a Q Exactive mass spectrometer (Thermo Scientific) that was coupled to an Easy nLC (Thermo Fisher Scientific) for 60 min.

    Article Title: Differential proteomic analysis of replanted Rehmannia glutinosa roots by iTRAQ reveals molecular mechanisms for formation of replant disease
    Article Snippet: .. Reverse-phase nanoflow HPLC and tandem mass spectrometry SCX fractions were dissolved in buffer A (0.1% formic acid) and loaded onto a reversed-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18). .. Peptides were separated with a linear gradient of buffer B (84% acetonitrile and 0.1% formic acid) at a flow rate of 300 nl/min.

    Article Title: Transcriptomic and proteomic analysis reveals mechanisms of low pollen-pistil compatibility during water lily cross breeding
    Article Snippet: We first loaded the peptide mixture onto reversed-phase trap column (Thermo Fisher Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18) and connected it to C18-reversed phase analytical column (Thermo Fisher Scientific Easy Column, 75 μm inner diameter, 10 cm long, 3 μm, C18-A2) in buffer A (0.1% formic acid), and then separated it with the linear gradient of buffer B (0.1% formic acid, 84% acetonitrile) under the flow rate of 300 nL / min controlled by intelliFlow technology. .. The LC-MS/MS analysis was carried out on the Q Exactive mass spectrometer (Thermo Fisher Scientific) coupled with a Easy nLC (Proxeon Biosystems, now Thermo Fisher Scientific) for 240 min. Mass spectrometer was operated under positive ion mode.

    Article Title: Mutations in the pantothenate kinase of Plasmodium falciparum confer diverse sensitivity profiles to antiplasmodial pantothenate analogues
    Article Snippet: Briefly, samples were loaded at a flow rate of 15 μ L/min onto a reversed-phase trap column (100 μ m × 2 cm; Acclaim PepMap media, Dionex), which was maintained at a temperature of 40°C. .. The mass spectrometer was operated in a data-independent acquisition (DIA) mode with a 25-fixed-window setup of 24 m/z effective precursor isolation over the m/z range of 375–975 Da.

    Tandem Mass Spectroscopy:

    Article Title: Comprehensive Annotation of the Parastagonospora nodorum Reference Genome Using Next-Generation Genomics, Transcriptomics and Proteogenomics
    Article Snippet: Acidified Offgel fractions were loaded onto a reversed-phase trap column (Dionex Acclaim PepMap μ-Precolumn C18, 0.3 mm x 5 mm) at 30 μL/min in 100% eluent A for 3.5 minutes and subsequently separated on a reversed phase capillary column (Vydac Everest C18 5μm 300 Å, 150 μm x150 mm, Alltech) at 45°C and a flow rate of 1 μL/min. .. Data was either acquired on an LTQ Orbitrap Velos as outlined below or an LTQ Orbitrap XL as described in Hastie et al. [ ] with the following modifications to data acquisition: target value of 1 x 103 for ion trap MS/MS scans; dynamic exclusion set to 70 s; ion selection threshold 1000 counts.

    Article Title: Transcriptomic and proteomic analysis reveals mechanisms of low pollen-pistil compatibility during water lily cross breeding
    Article Snippet: Paragraph title: MS/MS protein identification and quantification ... We first loaded the peptide mixture onto reversed-phase trap column (Thermo Fisher Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18) and connected it to C18-reversed phase analytical column (Thermo Fisher Scientific Easy Column, 75 μm inner diameter, 10 cm long, 3 μm, C18-A2) in buffer A (0.1% formic acid), and then separated it with the linear gradient of buffer B (0.1% formic acid, 84% acetonitrile) under the flow rate of 300 nL / min controlled by intelliFlow technology.

    Sequencing:

    Article Title: Mutations in the pantothenate kinase of Plasmodium falciparum confer diverse sensitivity profiles to antiplasmodial pantothenate analogues
    Article Snippet: For sample processing, a total of 500 μ g of protein, accurately determined using the Pierce BCA protein assay kit (ThermoFisher), was incubated overnight with sequencing-grade trypsin (1:50 dilution; Promega). .. Briefly, samples were loaded at a flow rate of 15 μ L/min onto a reversed-phase trap column (100 μ m × 2 cm; Acclaim PepMap media, Dionex), which was maintained at a temperature of 40°C.

    Article Title: Mutations in the pantothenate kinase of Plasmodium falciparum confer diverse sensitivity profiles to antiplasmodial pantothenate analogues
    Article Snippet: For sample processing, a total of 500 μ g of protein, accurately determined using the Pierce BCA protein assay kit (ThermoFisher), was incubated overnight with sequencing-grade trypsin (1:50 dilution; Promega). .. Briefly, samples were loaded at a flow rate of 15 μ L/min onto a reversed-phase trap column (100 μ m × 2 cm; Acclaim PepMap media, Dionex), which was maintained at a temperature of 40°C.

    Sonication:

    Article Title: Comparative Transcriptomic and Proteomic Analyses Identify Key Genes Associated With Milk Fat Traits in Chinese Holstein Cows
    Article Snippet: The homogenate was boiled for 3 min and then sonicated for 2 min. After centrifugation at 20,000 × g for 20 min at 4°C, the concentration of proteins in the filtrate was quantified with a BCA Protein Assay Kit (Bio-Rad, Hercules, CA, USA). .. The peptide mixture was loaded onto a reversed-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18) connected to a C18 reversed-phase analytical column (length = 10 cm, inner diameter = 75-μm, 3-μm resin; Thermo Fisher Scientific) in buffer A (0.1% formic acid) and separated for 1.5 h with a linear gradient of buffer B (98% acetonitrile and 0.1% formic acid) at a flow rate of 300 nL/min controlled by IntelliFlow technology (4%–7% buffer B for 2 min, 7%–20% buffer B for 65 min, 20%–35% buffer B for 12 min, 35%–90% buffer B for 2 min, and holding in 90% buffer B for 9 min).

    Injection:

    Article Title: Comprehensive Annotation of the Parastagonospora nodorum Reference Genome Using Next-Generation Genomics, Transcriptomics and Proteogenomics
    Article Snippet: For LC-MS/MS analysis, trypsin-digested samples were centrifuged for 5 min at 20,000 x g and an aliquot of 5 μL (Offgel fractions) or 3 μL (CWM proteome) was diluted to a volume of 20 μL with 6.5% formic acid prior to injection. .. Acidified Offgel fractions were loaded onto a reversed-phase trap column (Dionex Acclaim PepMap μ-Precolumn C18, 0.3 mm x 5 mm) at 30 μL/min in 100% eluent A for 3.5 minutes and subsequently separated on a reversed phase capillary column (Vydac Everest C18 5μm 300 Å, 150 μm x150 mm, Alltech) at 45°C and a flow rate of 1 μL/min.

    Article Title: iTRAQ Proteomic Analysis of Continuously Cropped Soybean Root Inoculated With Funneliformis mosseae
    Article Snippet: .. LC-MS/MS Analysis For nanoLC-MS/MS analysis, each peptide mixture was injected onto a reversed-phase trap column (Thermo Scientific Acclaim PepMap100; 100 μm × 2 cm, nanoViper C18) connected to a C18 reversed-phase analytical column (Thermo Scientific Easy Column; 10 cm long, 75 μm inner diameter, 3 μm resin) equilibrated in buffer A (0.1% formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% formic acid) at a flow rate of 300 nl/min controlled using IntelliFlow technology. ..

    Article Title: Transcriptomic and proteomic analysis reveals mechanisms of low pollen-pistil compatibility during water lily cross breeding
    Article Snippet: We first loaded the peptide mixture onto reversed-phase trap column (Thermo Fisher Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18) and connected it to C18-reversed phase analytical column (Thermo Fisher Scientific Easy Column, 75 μm inner diameter, 10 cm long, 3 μm, C18-A2) in buffer A (0.1% formic acid), and then separated it with the linear gradient of buffer B (0.1% formic acid, 84% acetonitrile) under the flow rate of 300 nL / min controlled by intelliFlow technology. .. The automatic gain control target, the maximum injection time and dynamic exclusion duration was set to 1e6 , 50 ms and 60.0 s respectively.

    Modification:

    Article Title: Comprehensive Annotation of the Parastagonospora nodorum Reference Genome Using Next-Generation Genomics, Transcriptomics and Proteogenomics
    Article Snippet: Samples were incubated at 37°C for 2 hours followed by the addition of further 1 μg of modified trypsin and 6 hours (Offgel fractions) and 14 hours (CWM proteome) digestion at 37°C. .. Acidified Offgel fractions were loaded onto a reversed-phase trap column (Dionex Acclaim PepMap μ-Precolumn C18, 0.3 mm x 5 mm) at 30 μL/min in 100% eluent A for 3.5 minutes and subsequently separated on a reversed phase capillary column (Vydac Everest C18 5μm 300 Å, 150 μm x150 mm, Alltech) at 45°C and a flow rate of 1 μL/min.

    Liquid Chromatography:

    Article Title: Neuropeptide Release Is Impaired in a Mouse Model of Fragile X Mental Retardation Syndrome
    Article Snippet: Paragraph title: Liquid Chromatography ... Briefly, the samples were initially loaded onto a 5-μL loop using a manual injector (VALCO Instruments Co. Inc., Houston, TX) and subsequently trapped onto a reversed-phase trap column (PepMap, C18, 5 μm, 100 Å, LC Packings, a Dionex Company, Sunnyvale, CA) using solvent C (90% water, 10% acetonitrile (ACN), 0.1% formic acid (FA), and 0.01% trifluoroacetic acid (TFA)) at a flow rate of 8 μL/min and washed for 5 min. Each sample was then eluted and separated on a reversed-phase column (LC Packings 300-μm i.d.

    Activity Assay:

    Article Title: Mutations in the pantothenate kinase of Plasmodium falciparum confer diverse sensitivity profiles to antiplasmodial pantothenate analogues
    Article Snippet: On the following day, trypsin activity was quenched using 5% formic acid (FA) and the detergent (sodium deoxycholate) used for protein solubilisation was removed. .. Briefly, samples were loaded at a flow rate of 15 μ L/min onto a reversed-phase trap column (100 μ m × 2 cm; Acclaim PepMap media, Dionex), which was maintained at a temperature of 40°C.

    Article Title: Mutations in the pantothenate kinase of Plasmodium falciparum confer diverse sensitivity profiles to antiplasmodial pantothenate analogues
    Article Snippet: On the following day, trypsin activity was quenched using 5% formic acid (FA) and the detergent (sodium deoxycholate) used for protein solubilisation was removed. .. Briefly, samples were loaded at a flow rate of 15 μ L/min onto a reversed-phase trap column (100 μ m × 2 cm; Acclaim PepMap media, Dionex), which was maintained at a temperature of 40°C.

    Generated:

    Article Title: Neuropeptide Release Is Impaired in a Mouse Model of Fragile X Mental Retardation Syndrome
    Article Snippet: Briefly, the samples were initially loaded onto a 5-μL loop using a manual injector (VALCO Instruments Co. Inc., Houston, TX) and subsequently trapped onto a reversed-phase trap column (PepMap, C18, 5 μm, 100 Å, LC Packings, a Dionex Company, Sunnyvale, CA) using solvent C (90% water, 10% acetonitrile (ACN), 0.1% formic acid (FA), and 0.01% trifluoroacetic acid (TFA)) at a flow rate of 8 μL/min and washed for 5 min. Each sample was then eluted and separated on a reversed-phase column (LC Packings 300-μm i.d. .. × 15 cm, C18 PepMap100, 100 Å) using a gradient at 2 μL/min flow rate for 45 min. Gradients were generated using solvent A (95% water, 5% ACN, 0.1% TFA) and solvent B (95% ACN, 5% water, 0.1% FA, and 0.01% TFA).

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    Thermo Fisher reverse phase trap column
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    Thermo Fisher reversed phase trap column
    Reversed Phase Trap Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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