reverse transcription quantitative pcr rt qpcr trizol  (Thermo Fisher)


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    Thermo Fisher reverse transcription quantitative pcr rt qpcr trizol
    BMECs promote the invasion and migration of HCC cells through CX3CL1. (A) Protein and (B) mRNA expression levels of CX3CL1 in HUVECs and BMECs cells were examined. <t>RT-qPCR</t> and western blot analysis data were analyzed using a Student's t-test following log transformation. (C) ELISA was used to examine secreted CX3CL1 expression in BMECs co-cultured with MHCC97H cells. (D) mRNA expression levels of CXCL8 , IL6 , CCL3 , MMP2 , MMP8 and PTHLH in MHCC97H cells. n=3. Hep3B and MHCC97H cells were co-cultured with BMECs in Transwell chambers. CX3CL1 antibody was used to neutralize the free CX3CL1 in medium. Western-blot and <t>PCR</t> data were analyzed using a Student's t-test following log transformation. (E) Representative images and statistical analysis of Transwell invasion assays showed the invasive ability of Hep3B and MHCC97H cells co-cultured with or without BMECs after 24 h; n=3. The number of the cells which had invaded was quantified. Scale bar, 200 μ m. (F) Representative images and analysis of wound healing assays showed the migratory ability of Hep3B and MHCC97H cells co-cultured with or without BMECs after 24 h. Migration distance of cells was quantified; n=3; scale bar, 200 μ m. Cell migration and invasion were analyzed using ANOVA test; n=3. BMECs, bone marrow epithelial cells; HCC, hepatocellular carcinoma; CX3CL1, C-X3-C motif chemokine ligand 1.
    Reverse Transcription Quantitative Pcr Rt Qpcr Trizol, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription quantitative pcr rt qpcr trizol/product/Thermo Fisher
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    reverse transcription quantitative pcr rt qpcr trizol - by Bioz Stars, 2020-07
    93/100 stars

    Images

    1) Product Images from "ADAM17-regulated CX3CL1 expression produced by bone marrow endothelial cells promotes spinal metastasis from hepatocellular carcinoma"

    Article Title: ADAM17-regulated CX3CL1 expression produced by bone marrow endothelial cells promotes spinal metastasis from hepatocellular carcinoma

    Journal: International Journal of Oncology

    doi: 10.3892/ijo.2020.5045

    BMECs promote the invasion and migration of HCC cells through CX3CL1. (A) Protein and (B) mRNA expression levels of CX3CL1 in HUVECs and BMECs cells were examined. RT-qPCR and western blot analysis data were analyzed using a Student's t-test following log transformation. (C) ELISA was used to examine secreted CX3CL1 expression in BMECs co-cultured with MHCC97H cells. (D) mRNA expression levels of CXCL8 , IL6 , CCL3 , MMP2 , MMP8 and PTHLH in MHCC97H cells. n=3. Hep3B and MHCC97H cells were co-cultured with BMECs in Transwell chambers. CX3CL1 antibody was used to neutralize the free CX3CL1 in medium. Western-blot and PCR data were analyzed using a Student's t-test following log transformation. (E) Representative images and statistical analysis of Transwell invasion assays showed the invasive ability of Hep3B and MHCC97H cells co-cultured with or without BMECs after 24 h; n=3. The number of the cells which had invaded was quantified. Scale bar, 200 μ m. (F) Representative images and analysis of wound healing assays showed the migratory ability of Hep3B and MHCC97H cells co-cultured with or without BMECs after 24 h. Migration distance of cells was quantified; n=3; scale bar, 200 μ m. Cell migration and invasion were analyzed using ANOVA test; n=3. BMECs, bone marrow epithelial cells; HCC, hepatocellular carcinoma; CX3CL1, C-X3-C motif chemokine ligand 1.
    Figure Legend Snippet: BMECs promote the invasion and migration of HCC cells through CX3CL1. (A) Protein and (B) mRNA expression levels of CX3CL1 in HUVECs and BMECs cells were examined. RT-qPCR and western blot analysis data were analyzed using a Student's t-test following log transformation. (C) ELISA was used to examine secreted CX3CL1 expression in BMECs co-cultured with MHCC97H cells. (D) mRNA expression levels of CXCL8 , IL6 , CCL3 , MMP2 , MMP8 and PTHLH in MHCC97H cells. n=3. Hep3B and MHCC97H cells were co-cultured with BMECs in Transwell chambers. CX3CL1 antibody was used to neutralize the free CX3CL1 in medium. Western-blot and PCR data were analyzed using a Student's t-test following log transformation. (E) Representative images and statistical analysis of Transwell invasion assays showed the invasive ability of Hep3B and MHCC97H cells co-cultured with or without BMECs after 24 h; n=3. The number of the cells which had invaded was quantified. Scale bar, 200 μ m. (F) Representative images and analysis of wound healing assays showed the migratory ability of Hep3B and MHCC97H cells co-cultured with or without BMECs after 24 h. Migration distance of cells was quantified; n=3; scale bar, 200 μ m. Cell migration and invasion were analyzed using ANOVA test; n=3. BMECs, bone marrow epithelial cells; HCC, hepatocellular carcinoma; CX3CL1, C-X3-C motif chemokine ligand 1.

    Techniques Used: Migration, Expressing, Quantitative RT-PCR, Western Blot, Transformation Assay, Enzyme-linked Immunosorbent Assay, Cell Culture, Polymerase Chain Reaction

    Related Articles

    Polymerase Chain Reaction:

    Article Title: ADAM17-regulated CX3CL1 expression produced by bone marrow endothelial cells promotes spinal metastasis from hepatocellular carcinoma
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) TRIzol® reagent was used to extract total RNA and a Thermo Script RT-PCR system was used to reverse transcribe the RNA into cDNA (cat. no. 12594100; Invitrogen; Thermo Fisher Scientific, Inc.). .. SYBR Premix Ex Taq™ II (Takara Bio, Inc.) was used to measure mRNA expression, and GAPDH was used as the endogenous reference gene.

    Article Title: Excessive production of mitochondrion-derived reactive oxygen species induced by titanium ions leads to autophagic cell death of osteoblasts via the SIRT3/SOD2 pathway
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) TRIzol® (Invitrogen; Thermo Fisher Scientific, Inc.) was used to extract RNA from osteoblastic cells, and its concentration and purity were detected with a UV spectrophotometer. cDNA was synthesized using a Sensiscript RT kit (Thermo Fisher Scientific, Inc.). .. All reagents used for RT-qPCR to detect SIRT3 mRNA were obtained from Thermo Fisher Scientific, Inc.

    Article Title: miR-155 promotes fibroblast-like synoviocyte proliferation and inflammatory cytokine secretion in rheumatoid arthritis by targeting FOXO3a
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) Trizol® (Invitrogen; Thermo Fisher Scientific, Inc.) was used to extract total RNA from tissues or cells. .. The extracted RNA was reverse transcribed to cDNA using a PrimeScript™ RT Master Mix reverse transcription kit (cat. no. RR036B, Takara Biotechnology Co., Ltd.) which was performed according to the manufacturer's protocol.

    Article Title: MicroRNA-449b-3p inhibits epithelial-mesenchymal transition by targeting IL-6 and through the JAK2/STAT3 signaling pathway in non-small cell lung cancer
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) TRIzol® reagent from Invitrogen; Thermo Fisher Scientific, Inc. was employed for isolating total RNA from samples, in accordance with the manufacturer's protocols. .. Total RNA was converted into first-strand cDNA using a Mir-X™ miRNA First-Strand Synthesis kit (Takara Bio, Inc.) for miRNAs or PrimeScript™ RT Reagent kit (Takara Bio, Inc.) for mRNAs per the prescribed protocols of the manufacturer.

    Synthesized:

    Article Title: Excessive production of mitochondrion-derived reactive oxygen species induced by titanium ions leads to autophagic cell death of osteoblasts via the SIRT3/SOD2 pathway
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) TRIzol® (Invitrogen; Thermo Fisher Scientific, Inc.) was used to extract RNA from osteoblastic cells, and its concentration and purity were detected with a UV spectrophotometer. cDNA was synthesized using a Sensiscript RT kit (Thermo Fisher Scientific, Inc.). .. All reagents used for RT-qPCR to detect SIRT3 mRNA were obtained from Thermo Fisher Scientific, Inc.

    Quantitative RT-PCR:

    Article Title: ADAM17-regulated CX3CL1 expression produced by bone marrow endothelial cells promotes spinal metastasis from hepatocellular carcinoma
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) TRIzol® reagent was used to extract total RNA and a Thermo Script RT-PCR system was used to reverse transcribe the RNA into cDNA (cat. no. 12594100; Invitrogen; Thermo Fisher Scientific, Inc.). .. SYBR Premix Ex Taq™ II (Takara Bio, Inc.) was used to measure mRNA expression, and GAPDH was used as the endogenous reference gene.

    Article Title: Excessive production of mitochondrion-derived reactive oxygen species induced by titanium ions leads to autophagic cell death of osteoblasts via the SIRT3/SOD2 pathway
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) TRIzol® (Invitrogen; Thermo Fisher Scientific, Inc.) was used to extract RNA from osteoblastic cells, and its concentration and purity were detected with a UV spectrophotometer. cDNA was synthesized using a Sensiscript RT kit (Thermo Fisher Scientific, Inc.). .. All reagents used for RT-qPCR to detect SIRT3 mRNA were obtained from Thermo Fisher Scientific, Inc.

    Article Title: miR-155 promotes fibroblast-like synoviocyte proliferation and inflammatory cytokine secretion in rheumatoid arthritis by targeting FOXO3a
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) Trizol® (Invitrogen; Thermo Fisher Scientific, Inc.) was used to extract total RNA from tissues or cells. .. The extracted RNA was reverse transcribed to cDNA using a PrimeScript™ RT Master Mix reverse transcription kit (cat. no. RR036B, Takara Biotechnology Co., Ltd.) which was performed according to the manufacturer's protocol.

    Article Title: MicroRNA-449b-3p inhibits epithelial-mesenchymal transition by targeting IL-6 and through the JAK2/STAT3 signaling pathway in non-small cell lung cancer
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) TRIzol® reagent from Invitrogen; Thermo Fisher Scientific, Inc. was employed for isolating total RNA from samples, in accordance with the manufacturer's protocols. .. Total RNA was converted into first-strand cDNA using a Mir-X™ miRNA First-Strand Synthesis kit (Takara Bio, Inc.) for miRNAs or PrimeScript™ RT Reagent kit (Takara Bio, Inc.) for mRNAs per the prescribed protocols of the manufacturer.

    Concentration Assay:

    Article Title: Excessive production of mitochondrion-derived reactive oxygen species induced by titanium ions leads to autophagic cell death of osteoblasts via the SIRT3/SOD2 pathway
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) TRIzol® (Invitrogen; Thermo Fisher Scientific, Inc.) was used to extract RNA from osteoblastic cells, and its concentration and purity were detected with a UV spectrophotometer. cDNA was synthesized using a Sensiscript RT kit (Thermo Fisher Scientific, Inc.). .. All reagents used for RT-qPCR to detect SIRT3 mRNA were obtained from Thermo Fisher Scientific, Inc.

    Spectrophotometry:

    Article Title: Excessive production of mitochondrion-derived reactive oxygen species induced by titanium ions leads to autophagic cell death of osteoblasts via the SIRT3/SOD2 pathway
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) TRIzol® (Invitrogen; Thermo Fisher Scientific, Inc.) was used to extract RNA from osteoblastic cells, and its concentration and purity were detected with a UV spectrophotometer. cDNA was synthesized using a Sensiscript RT kit (Thermo Fisher Scientific, Inc.). .. All reagents used for RT-qPCR to detect SIRT3 mRNA were obtained from Thermo Fisher Scientific, Inc.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: ADAM17-regulated CX3CL1 expression produced by bone marrow endothelial cells promotes spinal metastasis from hepatocellular carcinoma
    Article Snippet: .. Reverse transcription-quantitative PCR (RT-qPCR) TRIzol® reagent was used to extract total RNA and a Thermo Script RT-PCR system was used to reverse transcribe the RNA into cDNA (cat. no. 12594100; Invitrogen; Thermo Fisher Scientific, Inc.). .. SYBR Premix Ex Taq™ II (Takara Bio, Inc.) was used to measure mRNA expression, and GAPDH was used as the endogenous reference gene.

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    Thermo Fisher reverse transcription quantitative pcr rt qpcr trizol reagent
    Reverse Transcription Quantitative Pcr Rt Qpcr Trizol Reagent, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse transcription quantitative pcr rt qpcr trizol reagent/product/Thermo Fisher
    Average 99 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    reverse transcription quantitative pcr rt qpcr trizol reagent - by Bioz Stars, 2020-07
    99/100 stars
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