reverse phase trap column  (Thermo Fisher)


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    Thermo Fisher reverse phase trap column
    Reverse Phase Trap Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse phase trap column/product/Thermo Fisher
    Average 96 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    reverse phase trap column - by Bioz Stars, 2020-04
    96/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Proteomics‐based screening of the target proteins associated with antidepressant‐like effect and mechanism of Saikosaponin A, et al. Proteomics‐based screening of the target proteins associated with antidepressant‐like effect and mechanism of Saikosaponin A
    Article Snippet: Paragraph title: HPLC and LC‐MS/MS analysis ... The peptide mixture was loaded onto a reverse‐phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18) connected to the C18 reversed‐phase analytical column (Thermo Scientific Easy Column, 10 cm long, 75 μm inner diameter, 3 μm resin), followed by a mobile phase elution with buffer A (0.1% formic acid) and buffer B (84% acetonitrile and 0.1% formic acid).

    Flow Cytometry:

    Article Title: Comparative Proteomic Analysis of Pleurotus ostreatus Reveals Great Metabolic Differences in the Cap and Stipe Development and the Potential Role of Ca2+ in the Primordium Differentiation
    Article Snippet: .. The peptide mixture was loaded onto a reverse-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18, Waltham, MA, USA) connected to the C18 reversed-phase analytical column (Thermo Scientific Easy Column, 10 cm long, 75 μm inner diameter, 3μm resin) in buffer A (0.1% formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% formic acid), at a flow rate of 300 nl/min controlled by IntelliFlow technology. .. The total time of each fraction was 65 min. Mass spectrometry analysis was performed on a Q Exactive mass spectrometer (Thermo Scientific) that was coupled to Easy nLC (Proxeon Biosystems, now Thermo Fisher Scientific) for 65 min.

    Article Title: Proteomics integrated with metabolomics: analysis of the internal causes of nutrient changes in alfalfa at different growth stages
    Article Snippet: .. The peptide mixture was loaded onto a reverse-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18) connected to a C18 reverse-phase analytical column (Thermo Scientific Easy Column, 10 cm long, 75 μm inner diameter, 3 μm resin) in buffer A (0.1% formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% formic acid) at a flow rate of 300 nl/min, controlled by IntelliFlow technology. .. The analysis gradient was a 1-h gradient consisting of 0–50% buffer B for 50 min, 50–100% buffer B for 5 min, and holding in 100% buffer B for 5 min. LC-MS/MS analysis was performed on a Q Exactive mass spectrometer (Thermo Scientific) coupled to an Easy nLC system (Proxeon Biosystems, now Thermo Fisher Scientific) for 60 min.

    Article Title: Arenobufagin induces MCF-7 cell apoptosis by promoting JNK-mediated multisite phosphorylation of Yes-associated protein
    Article Snippet: .. Peptide mixtures were loaded onto a reverse-phase trap column (100 μm × 2 cm, nanoViper C18, Thermo Scientific Acclaim PepMap100) connected to the C18 reversed-phase analytical column (75 μm × 10 cm, 3 μm, Thermo Scientific Easy Column) in buffer A (0.1% FA) and separated with a linear gradient of buffer B (84% ACN and 0.1% FA) at a flow rate of 300 nL/min. .. The Q Exactive spectrometer was operated in positive ion mode, and survey scan MS spectra (from m/z 300–1800) were acquired at a resolution of 70,000.

    Article Title: Quantitative analysis of the grain amyloplast proteome reveals differences in metabolism between two wheat cultivars at two stages of grain development
    Article Snippet: .. The peptide mixture was loaded onto a reverse-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18) connected to the C18-reverse phase analytical column (Thermo Scientific Easy Column, 10 cm long, 75 μm inner diameter, 3 μm, C18-A2) in buffer A (0.1% formic acid) and separated with a linear gradient of buffer B (84% acetonitrile, 0.1% formic acid) at a flow rate of 300 nl/min controlled by IntelliFlow technology. .. LC-MS/MS analysis was performed on a Q Exactive mass spectrometer (Thermo Scientific) coupled to an Easy nLC (Proxeon Biosystems, now ThermoFisher Scientific) for 240 min.

    Article Title: Proteomics‐based screening of the target proteins associated with antidepressant‐like effect and mechanism of Saikosaponin A, et al. Proteomics‐based screening of the target proteins associated with antidepressant‐like effect and mechanism of Saikosaponin A
    Article Snippet: The peptide mixture was loaded onto a reverse‐phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18) connected to the C18 reversed‐phase analytical column (Thermo Scientific Easy Column, 10 cm long, 75 μm inner diameter, 3 μm resin), followed by a mobile phase elution with buffer A (0.1% formic acid) and buffer B (84% acetonitrile and 0.1% formic acid). .. Peptides were then eluted in a linear gradient with buffer B from 0% to 100% over 60 min at a flow rate of 300 nL/min.

    Article Title: Expression Profile of the Digestive Enzymes of Manisjavanica Reveals Its Adaptation to Diet Specialization
    Article Snippet: .. The peptide mixture was loaded onto a reverse-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18; Thermo Fisher Scientific, Waltham, MA, USA) connected to a C18 reversed-phase analytical column (Thermo Scientific EASY-Column, 10 cm long, 75 μm inner diameter, 3 μm resin; Thermo Fisher Scientific) in buffer A (0.1% formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% formic acid) at a flow rate of 300 nL/min, controlled by IntelliFlow technology. .. The linear gradient was as follows: 0–35% buffer B for 50 min, 35–1100% buffer B for 5 min, and holding in 100% buffer B for 5 min. LC–MS/MS was performed on a Q Exactive mass spectrometer (Thermo Fisher Scientific) coupled to an EASY-nLC device [Proxeon Biosystems (Thermo Fisher Scientific)] for 60 min.

    Article Title: Determination of the Role of Microcystis aeruginosa in Toxin Generation Based on Phosphoproteomic Profiles
    Article Snippet: .. The peptide mixture was loaded onto a reverse-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18) that was connected to a C18-reversed phase analytical column (Thermo Scientific Easy Column, 10-cm long, 75-μm inner diameter, 3-μm resin) in buffer A (0.1% formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% formic acid) at a flow rate of 300 nL/min for 2 h. The LC-MS/MS system was based on an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific, Waltham, MA, USA), and the analysis was performed as previously described [ ] . .. The mass spectrometer was operated in the positive ion mode and acquired the 10 most abundant precursor ions from each survey scan.

    Selection:

    Article Title: Expression Profile of the Digestive Enzymes of Manisjavanica Reveals Its Adaptation to Diet Specialization
    Article Snippet: The peptide mixture was loaded onto a reverse-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18; Thermo Fisher Scientific, Waltham, MA, USA) connected to a C18 reversed-phase analytical column (Thermo Scientific EASY-Column, 10 cm long, 75 μm inner diameter, 3 μm resin; Thermo Fisher Scientific) in buffer A (0.1% formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% formic acid) at a flow rate of 300 nL/min, controlled by IntelliFlow technology. .. MS data were acquired using a data-dependent top 10 method, with dynamic selection of the most abundant precursor ions from the survey scan (300–1800 m /z ) for HCD fragmentation.

    Mass Spectrometry:

    Article Title: Comparative Proteomic Analysis of Pleurotus ostreatus Reveals Great Metabolic Differences in the Cap and Stipe Development and the Potential Role of Ca2+ in the Primordium Differentiation
    Article Snippet: The peptide mixture was loaded onto a reverse-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18, Waltham, MA, USA) connected to the C18 reversed-phase analytical column (Thermo Scientific Easy Column, 10 cm long, 75 μm inner diameter, 3μm resin) in buffer A (0.1% formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% formic acid), at a flow rate of 300 nl/min controlled by IntelliFlow technology. .. The total time of each fraction was 65 min. Mass spectrometry analysis was performed on a Q Exactive mass spectrometer (Thermo Scientific) that was coupled to Easy nLC (Proxeon Biosystems, now Thermo Fisher Scientific) for 65 min.

    Article Title: Proteomics integrated with metabolomics: analysis of the internal causes of nutrient changes in alfalfa at different growth stages
    Article Snippet: Paragraph title: Mass spectrometry ... The peptide mixture was loaded onto a reverse-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18) connected to a C18 reverse-phase analytical column (Thermo Scientific Easy Column, 10 cm long, 75 μm inner diameter, 3 μm resin) in buffer A (0.1% formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% formic acid) at a flow rate of 300 nl/min, controlled by IntelliFlow technology.

    Article Title: Arenobufagin induces MCF-7 cell apoptosis by promoting JNK-mediated multisite phosphorylation of Yes-associated protein
    Article Snippet: LC–MS analysis The lyophilized peptides were reconstituted in 20 μL of 0.1% FA and 6 μL of the sample was analyzed by an online reverse-phase LC–MS/MS system consisting of an Eksigent NanoLCUltra 2D plus system (AB SCIEX) coupled with a Q Exactive mass spectrometer (Thermo Fisher Scientific, CA, USA) via a nanoelectrospray source. .. Peptide mixtures were loaded onto a reverse-phase trap column (100 μm × 2 cm, nanoViper C18, Thermo Scientific Acclaim PepMap100) connected to the C18 reversed-phase analytical column (75 μm × 10 cm, 3 μm, Thermo Scientific Easy Column) in buffer A (0.1% FA) and separated with a linear gradient of buffer B (84% ACN and 0.1% FA) at a flow rate of 300 nL/min.

    Article Title: Quantitative analysis of the grain amyloplast proteome reveals differences in metabolism between two wheat cultivars at two stages of grain development
    Article Snippet: The peptide mixture was loaded onto a reverse-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18) connected to the C18-reverse phase analytical column (Thermo Scientific Easy Column, 10 cm long, 75 μm inner diameter, 3 μm, C18-A2) in buffer A (0.1% formic acid) and separated with a linear gradient of buffer B (84% acetonitrile, 0.1% formic acid) at a flow rate of 300 nl/min controlled by IntelliFlow technology. .. LC-MS/MS analysis was performed on a Q Exactive mass spectrometer (Thermo Scientific) coupled to an Easy nLC (Proxeon Biosystems, now ThermoFisher Scientific) for 240 min.

    Article Title: Proteomics‐based screening of the target proteins associated with antidepressant‐like effect and mechanism of Saikosaponin A, et al. Proteomics‐based screening of the target proteins associated with antidepressant‐like effect and mechanism of Saikosaponin A
    Article Snippet: The peptide mixture was loaded onto a reverse‐phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18) connected to the C18 reversed‐phase analytical column (Thermo Scientific Easy Column, 10 cm long, 75 μm inner diameter, 3 μm resin), followed by a mobile phase elution with buffer A (0.1% formic acid) and buffer B (84% acetonitrile and 0.1% formic acid). .. LC‐MS/MS analysis was performed on a Q Exactive mass spectrometer (Thermo Scientific) that was coupled to an Easy nLC (Proxeon Biosystems, now Thermo Fisher Scientific) for 60 min.

    Article Title: Proteomic and lipidomic analysis of exosomes derived from ovarian cancer cells and ovarian surface epithelial cells
    Article Snippet: The peptide mixture was loaded onto a reverse phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm*2 cm, nanoViper C18) connected to the C18-reversed phase analytical column (Thermo Scientific Easy Column, 10 cm long, 75 μm inner diameter, 3 μm resin) in buffer A (0.1% Formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% Formic acid) at a flow rate of 300 nl/min controlled by IntelliFlow technology. .. MS analysis was performed on a Q-Exactive mass spectrometer (Thermo Scientific) that was coupled to Easy nLC (Thermo Scientific) for 120 min.

    Article Title: Expression Profile of the Digestive Enzymes of Manisjavanica Reveals Its Adaptation to Diet Specialization
    Article Snippet: The peptide mixture was loaded onto a reverse-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18; Thermo Fisher Scientific, Waltham, MA, USA) connected to a C18 reversed-phase analytical column (Thermo Scientific EASY-Column, 10 cm long, 75 μm inner diameter, 3 μm resin; Thermo Fisher Scientific) in buffer A (0.1% formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% formic acid) at a flow rate of 300 nL/min, controlled by IntelliFlow technology. .. The linear gradient was as follows: 0–35% buffer B for 50 min, 35–1100% buffer B for 5 min, and holding in 100% buffer B for 5 min. LC–MS/MS was performed on a Q Exactive mass spectrometer (Thermo Fisher Scientific) coupled to an EASY-nLC device [Proxeon Biosystems (Thermo Fisher Scientific)] for 60 min.

    Article Title: Effects of Exogenous Synthetic Autoinducer-2 on Physiological Behaviors and Proteome of Lactic Acid Bacteria
    Article Snippet: .. Briefly, peptides were transferred into a reverse-phase trap column (Thermo Scientific Acclaim PepMap100, nanoViper C18, 100 μm × 2 cm) combined with a C18 reverse-phase column (10 cm long, 3 μm resin, 75 μm inner diameter, Thermo Scientific Easy Column) in buffer A and a linear gradient buffer B (0.1% formic acid and 84% acetonitrile) was used to separate at 300 nL/min over 120 min. A Q-Exactive mass spectrometer was combined with Easy nLC (Proxeon Biosystems, Thermo Fisher Scientific) for MS experiments. .. Positive ion mode was used in the mass spectrometer.

    Article Title: Determination of the Role of Microcystis aeruginosa in Toxin Generation Based on Phosphoproteomic Profiles
    Article Snippet: .. The peptide mixture was loaded onto a reverse-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18) that was connected to a C18-reversed phase analytical column (Thermo Scientific Easy Column, 10-cm long, 75-μm inner diameter, 3-μm resin) in buffer A (0.1% formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% formic acid) at a flow rate of 300 nL/min for 2 h. The LC-MS/MS system was based on an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific, Waltham, MA, USA), and the analysis was performed as previously described [ ] . .. The mass spectrometer was operated in the positive ion mode and acquired the 10 most abundant precursor ions from each survey scan.

    Chromatography:

    Article Title: Comparative Proteomic Analysis of Pleurotus ostreatus Reveals Great Metabolic Differences in the Cap and Stipe Development and the Potential Role of Ca2+ in the Primordium Differentiation
    Article Snippet: LC–MS/MS Analysis After fractionation, a total of 40 fractions were collected and subsequently pooled into 24 fractions according to the chromatography. .. The peptide mixture was loaded onto a reverse-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18, Waltham, MA, USA) connected to the C18 reversed-phase analytical column (Thermo Scientific Easy Column, 10 cm long, 75 μm inner diameter, 3μm resin) in buffer A (0.1% formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% formic acid), at a flow rate of 300 nl/min controlled by IntelliFlow technology.

    Centrifugation:

    Article Title: Proteomic and lipidomic analysis of exosomes derived from ovarian cancer cells and ovarian surface epithelial cells
    Article Snippet: The peptides of each sample were desalted on C18 Cartridges (Empore™ SPE Cartridges, Sigma), concentrated by vacuum centrifugation and reconstituted in 40 μl of 0.1% (v/v) formic acid. .. The peptide mixture was loaded onto a reverse phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm*2 cm, nanoViper C18) connected to the C18-reversed phase analytical column (Thermo Scientific Easy Column, 10 cm long, 75 μm inner diameter, 3 μm resin) in buffer A (0.1% Formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% Formic acid) at a flow rate of 300 nl/min controlled by IntelliFlow technology.

    Blocking Assay:

    Article Title: Proteomic and lipidomic analysis of exosomes derived from ovarian cancer cells and ovarian surface epithelial cells
    Article Snippet: Then 100 μl iodoacetamide (100 mM IAA in UA buffer) was added to block reduced cysteine residues and the samples were incubated for 30 min in darkness. .. The peptide mixture was loaded onto a reverse phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm*2 cm, nanoViper C18) connected to the C18-reversed phase analytical column (Thermo Scientific Easy Column, 10 cm long, 75 μm inner diameter, 3 μm resin) in buffer A (0.1% Formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% Formic acid) at a flow rate of 300 nl/min controlled by IntelliFlow technology.

    Article Title: Effects of Exogenous Synthetic Autoinducer-2 on Physiological Behaviors and Proteome of Lactic Acid Bacteria
    Article Snippet: Then, the UA buffer was used to block cysteine residues with the addition of 0.05 M iodoacetamide and incubated for 20 min in the dark. .. Briefly, peptides were transferred into a reverse-phase trap column (Thermo Scientific Acclaim PepMap100, nanoViper C18, 100 μm × 2 cm) combined with a C18 reverse-phase column (10 cm long, 3 μm resin, 75 μm inner diameter, Thermo Scientific Easy Column) in buffer A and a linear gradient buffer B (0.1% formic acid and 84% acetonitrile) was used to separate at 300 nL/min over 120 min. A Q-Exactive mass spectrometer was combined with Easy nLC (Proxeon Biosystems, Thermo Fisher Scientific) for MS experiments.

    Incubation:

    Article Title: Proteomic and lipidomic analysis of exosomes derived from ovarian cancer cells and ovarian surface epithelial cells
    Article Snippet: Then 100 μl iodoacetamide (100 mM IAA in UA buffer) was added to block reduced cysteine residues and the samples were incubated for 30 min in darkness. .. The peptide mixture was loaded onto a reverse phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm*2 cm, nanoViper C18) connected to the C18-reversed phase analytical column (Thermo Scientific Easy Column, 10 cm long, 75 μm inner diameter, 3 μm resin) in buffer A (0.1% Formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% Formic acid) at a flow rate of 300 nl/min controlled by IntelliFlow technology.

    Article Title: Effects of Exogenous Synthetic Autoinducer-2 on Physiological Behaviors and Proteome of Lactic Acid Bacteria
    Article Snippet: Finally, the protein suspension was incubated overnight along with trypsin (Promega) at 37 °C in 25 mM NH4 HCO3 . .. Briefly, peptides were transferred into a reverse-phase trap column (Thermo Scientific Acclaim PepMap100, nanoViper C18, 100 μm × 2 cm) combined with a C18 reverse-phase column (10 cm long, 3 μm resin, 75 μm inner diameter, Thermo Scientific Easy Column) in buffer A and a linear gradient buffer B (0.1% formic acid and 84% acetonitrile) was used to separate at 300 nL/min over 120 min. A Q-Exactive mass spectrometer was combined with Easy nLC (Proxeon Biosystems, Thermo Fisher Scientific) for MS experiments.

    Fractionation:

    Article Title: Comparative Proteomic Analysis of Pleurotus ostreatus Reveals Great Metabolic Differences in the Cap and Stipe Development and the Potential Role of Ca2+ in the Primordium Differentiation
    Article Snippet: LC–MS/MS Analysis After fractionation, a total of 40 fractions were collected and subsequently pooled into 24 fractions according to the chromatography. .. The peptide mixture was loaded onto a reverse-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18, Waltham, MA, USA) connected to the C18 reversed-phase analytical column (Thermo Scientific Easy Column, 10 cm long, 75 μm inner diameter, 3μm resin) in buffer A (0.1% formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% formic acid), at a flow rate of 300 nl/min controlled by IntelliFlow technology.

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Arenobufagin induces MCF-7 cell apoptosis by promoting JNK-mediated multisite phosphorylation of Yes-associated protein
    Article Snippet: Paragraph title: LC–MS analysis ... Peptide mixtures were loaded onto a reverse-phase trap column (100 μm × 2 cm, nanoViper C18, Thermo Scientific Acclaim PepMap100) connected to the C18 reversed-phase analytical column (75 μm × 10 cm, 3 μm, Thermo Scientific Easy Column) in buffer A (0.1% FA) and separated with a linear gradient of buffer B (84% ACN and 0.1% FA) at a flow rate of 300 nL/min.

    Tandem Mass Spectroscopy:

    Article Title: Quantitative analysis of the grain amyloplast proteome reveals differences in metabolism between two wheat cultivars at two stages of grain development
    Article Snippet: Paragraph title: MS/MS protein identification and quantification ... The peptide mixture was loaded onto a reverse-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18) connected to the C18-reverse phase analytical column (Thermo Scientific Easy Column, 10 cm long, 75 μm inner diameter, 3 μm, C18-A2) in buffer A (0.1% formic acid) and separated with a linear gradient of buffer B (84% acetonitrile, 0.1% formic acid) at a flow rate of 300 nl/min controlled by IntelliFlow technology.

    Article Title: Effects of Exogenous Synthetic Autoinducer-2 on Physiological Behaviors and Proteome of Lactic Acid Bacteria
    Article Snippet: Briefly, peptides were transferred into a reverse-phase trap column (Thermo Scientific Acclaim PepMap100, nanoViper C18, 100 μm × 2 cm) combined with a C18 reverse-phase column (10 cm long, 3 μm resin, 75 μm inner diameter, Thermo Scientific Easy Column) in buffer A and a linear gradient buffer B (0.1% formic acid and 84% acetonitrile) was used to separate at 300 nL/min over 120 min. A Q-Exactive mass spectrometer was combined with Easy nLC (Proxeon Biosystems, Thermo Fisher Scientific) for MS experiments. .. The 20 data-dependent MS/MS scans (AGC target 5e5 ) with a normalized collision energy of 30 eV were performed after a full FT scan mass spectrum cycle (the automatic gain control (AGC) target was 1e6 , and the HCD fragmentation was 300–1800 m /z ).

    Injection:

    Article Title: Comparative Proteomic Analysis of Pleurotus ostreatus Reveals Great Metabolic Differences in the Cap and Stipe Development and the Potential Role of Ca2+ in the Primordium Differentiation
    Article Snippet: Each fraction was injected for nano LC–MS/MS analysis. .. The peptide mixture was loaded onto a reverse-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18, Waltham, MA, USA) connected to the C18 reversed-phase analytical column (Thermo Scientific Easy Column, 10 cm long, 75 μm inner diameter, 3μm resin) in buffer A (0.1% formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% formic acid), at a flow rate of 300 nl/min controlled by IntelliFlow technology.

    Article Title: Proteomics integrated with metabolomics: analysis of the internal causes of nutrient changes in alfalfa at different growth stages
    Article Snippet: Mass spectrometry Each fraction was injected for nanoLC-MS/MS analysis. .. The peptide mixture was loaded onto a reverse-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18) connected to a C18 reverse-phase analytical column (Thermo Scientific Easy Column, 10 cm long, 75 μm inner diameter, 3 μm resin) in buffer A (0.1% formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% formic acid) at a flow rate of 300 nl/min, controlled by IntelliFlow technology.

    Article Title: Quantitative analysis of the grain amyloplast proteome reveals differences in metabolism between two wheat cultivars at two stages of grain development
    Article Snippet: Each fraction was injected for nanoLC-MS/MS analysis. .. The peptide mixture was loaded onto a reverse-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18) connected to the C18-reverse phase analytical column (Thermo Scientific Easy Column, 10 cm long, 75 μm inner diameter, 3 μm, C18-A2) in buffer A (0.1% formic acid) and separated with a linear gradient of buffer B (84% acetonitrile, 0.1% formic acid) at a flow rate of 300 nl/min controlled by IntelliFlow technology.

    Article Title: Expression Profile of the Digestive Enzymes of Manisjavanica Reveals Its Adaptation to Diet Specialization
    Article Snippet: Shotgun LC–MS/MS and Data Analysis Nano LC–MS/MS was performed on the products obtained from the enzymatic hydrolysis of 3 g of samples according to the quantitative results, and each sample was injected once. .. The peptide mixture was loaded onto a reverse-phase trap column (Thermo Scientific Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18; Thermo Fisher Scientific, Waltham, MA, USA) connected to a C18 reversed-phase analytical column (Thermo Scientific EASY-Column, 10 cm long, 75 μm inner diameter, 3 μm resin; Thermo Fisher Scientific) in buffer A (0.1% formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% formic acid) at a flow rate of 300 nL/min, controlled by IntelliFlow technology.

    Liquid Chromatography:

    Article Title: Effects of Exogenous Synthetic Autoinducer-2 on Physiological Behaviors and Proteome of Lactic Acid Bacteria
    Article Snippet: Liquid chromatography (Thermo Scientific) was used for LC–MS/MS and coupled with a Q-Exactive mass spectrometer (Thermo Scientific) as previously reported. .. Briefly, peptides were transferred into a reverse-phase trap column (Thermo Scientific Acclaim PepMap100, nanoViper C18, 100 μm × 2 cm) combined with a C18 reverse-phase column (10 cm long, 3 μm resin, 75 μm inner diameter, Thermo Scientific Easy Column) in buffer A and a linear gradient buffer B (0.1% formic acid and 84% acetonitrile) was used to separate at 300 nL/min over 120 min. A Q-Exactive mass spectrometer was combined with Easy nLC (Proxeon Biosystems, Thermo Fisher Scientific) for MS experiments.

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  • 96
    Thermo Fisher reverse phase trap column
    Reverse Phase Trap Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reverse phase trap column/product/Thermo Fisher
    Average 96 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    reverse phase trap column - by Bioz Stars, 2020-04
    96/100 stars
      Buy from Supplier

    98
    Thermo Fisher reversed phase trap column
    Reversed Phase Trap Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reversed phase trap column/product/Thermo Fisher
    Average 98 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    reversed phase trap column - by Bioz Stars, 2020-04
    98/100 stars
      Buy from Supplier

    94
    Thermo Fisher acclaim pepmap reverse phase column
    Acclaim Pepmap Reverse Phase Column, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acclaim pepmap reverse phase column/product/Thermo Fisher
    Average 94 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    acclaim pepmap reverse phase column - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

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