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Waters Corporation reverse phase hplc column
Reverse Phase Hplc Column, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 97/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/reverse phase hplc column/product/Waters Corporation
Average 97 stars, based on 11 article reviews
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reverse phase hplc column - by Bioz Stars, 2020-04
97/100 stars

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Clone Assay:

Article Title: Modification of Tau by 8-Nitroguanosine 3′,5′-Cyclic Monophosphate (8-Nitro-cGMP)
Article Snippet: Wild-type three-repeat (2N3R) and four-repeat (2N4R) tau and their corresponding cysteine-to-alanine substituted mutants (C322A-2N3R, C291,322A-2N4R) were cloned into a pRK172 vector and purified by a modified method reported previously ( ). .. Tau was fractioned from the effluent by ammonium sulfate precipitation and loaded onto a reverse-phase HPLC column (COSMOSIL Protein-R Waters, Nacalai Tesque Inc.) following gel filtration chromatography to exchange buffers (NAP-10 column, GE Healthcare).

Centrifugation:

Article Title: Bioreactor culture of recombinant Drosophila melanogaster S2 cells: characterization of metabolic features related to cell growth and production of the rabies virus glycoprotein
Article Snippet: .. The concentrations of glucose, lactate and glutamine in the culture supernatants were determined enzymatically with a YSI Biochemical Analyzer (model 2700) after sample centrifugation at 200 g for 5 min. Amino acid concentrations in the culture supernatants were analyzed by the Pico-tag system, using a reverse-phase HPLC column (Waters Sweden, Sollentuna, Sweden) (Bidlingmeyer et al. ). ..

Article Title: A deep proteomics perspective on CRM1-mediated nuclear export and nucleocytoplasmic partitioning
Article Snippet: Following digestion, the samples were acidified with trifluoroacetic acid (final concentration of 1%, v/v, 37°C, 1 h) to break down the RapiGest surfactant, the resulting by-products were pelleted by centrifugation (13,000 rpm, 15 min, RT), and the supernatant containing the digested peptides was transferred to a new tube. .. Then, peptides were resuspended in 10 mM ammonium hydroxide (pH 10) and loaded onto a reverse phase HPLC column (XBridge C18, Waters, 3.5 µm, 1.0 mm x 150 mm) and eluted in a 5-35% (v/v) acetonitrile gradient at a flow rate of 60 µl/min.

Article Title: Modification of Tau by 8-Nitroguanosine 3′,5′-Cyclic Monophosphate (8-Nitro-cGMP)
Article Snippet: After centrifugation, the supernatant was loaded onto a phosphocellulose column (P11, Whatman), and tau was eluted with 0.3 m sodium chloride. .. Tau was fractioned from the effluent by ammonium sulfate precipitation and loaded onto a reverse-phase HPLC column (COSMOSIL Protein-R Waters, Nacalai Tesque Inc.) following gel filtration chromatography to exchange buffers (NAP-10 column, GE Healthcare).

Filtration:

Article Title: Pharmacokinetics of Cefepime in Patients with Thermal Burn Injury
Article Snippet: Chromatographic separation was performed with a reverse-phase HPLC column (Nova-Pak C-18; 3.9 by 150 mm; Waters). .. Dissolved gases were removed from the final product by filtration through a 0.45-μm-pore-size nylon membrane filter (Whatman, Maldstone, England) while the sample was stirred under vacuum.

Article Title: Modification of Tau by 8-Nitroguanosine 3′,5′-Cyclic Monophosphate (8-Nitro-cGMP)
Article Snippet: .. Tau was fractioned from the effluent by ammonium sulfate precipitation and loaded onto a reverse-phase HPLC column (COSMOSIL Protein-R Waters, Nacalai Tesque Inc.) following gel filtration chromatography to exchange buffers (NAP-10 column, GE Healthcare). .. Tau protein concentration was determined using a BCA protein assay kit (Pierce).

Incubation:

Article Title: Mechanism-Based Inactivation of CYP2B1 and Its F-Helix Mutant by Two tert-Butyl Acetylenic Compounds: Covalent Modification of Prosthetic Heme Versus Apoprotein
Article Snippet: .. An aliquot (25 pmol) of the primary reaction mixtures inactivated by incubation with BMP was injected onto a reverse-phase HPLC column (XTerra MS C18, 2.1 × 150 mm; Waters) equilibrated with 65% solvent A (0.05% TFA/0.05% formic acid) and 35% solvent B (0.05% TFA/0.05% formic acid in acetonitrile) at a flow rate of 0.3 ml/min. ..

Article Title: A single amino acid substitution in a chitinase of the legume Medicago truncatula is sufficient to gain Nod-factor hydrolase activity
Article Snippet: Enzyme assays with Nod-factors Defined amounts of purified proteins and NFs were incubated in 25 mM sodium acetate buffer (pH 5.0) at 37°C for an indicated time period. .. Samples were taken up by 1 µl DMSO followed by 60 µl 50% acetonitrile and loaded on a reverse phase HPLC column (Nova Pak C18, 3.9 × 150 mm, particle size 4 mm; Waters).

Article Title: ProTargetMiner as a proteome signature library of anticancer molecules for functional discovery
Article Snippet: TMT10 reagents (Thermo; Cat#90110) were added 4x by weight to each sample, followed by incubation for 2 h at room temperature. .. The deep proteomics samples (tags assigned in Supplementary Table ) were prepared according to the above protocol until the multiplexing, cleaning, and drying steps, after which the samples were resuspended in 20 mM ammonium hydroxide and separated into 96 fractions on an XBrigde BEH C18 2.1 × 150 mm column (Waters; Cat#186003023), using a Dionex Ultimate 3000 2DLC system (Thermo Scientific) over a 48 min gradient of 1–63%B (B = 20 mM ammonium hydroxide in acetonitrile) in three steps (1–23.5%B in 42 min, 23.5–54%B in 4 min and then 54–63%B in 2 min) at 200 µL min−1 flow.

Mass Spectrometry:

Article Title: Oral curcumin for Alzheimer's disease: tolerability and efficacy in a 24-week randomized, double blind, placebo-controlled study
Article Snippet: The LC/MS/MS system (Varian International, Lake Forest, CA, USA) consisted of the proStar 410 autosampler and pumps and a 310 mass spectrometer with ESI (-) which is able to optimize each ion automatically. .. Dried plasma/CSF extract samples were re-dissolved in acetonitrile:water (50/50, by volume) in 10 mM ammonium acetate, and an aliquot of the solution (typically 50 ul) was injected onto a reverse phase HPLC column (Atlantis T3, 150 × 2.1 mm; Waters Corporation, Milford, MA, USA).

Article Title: Mechanism-Based Inactivation of CYP2B1 and Its F-Helix Mutant by Two tert-Butyl Acetylenic Compounds: Covalent Modification of Prosthetic Heme Versus Apoprotein
Article Snippet: .. An aliquot (25 pmol) of the primary reaction mixtures inactivated by incubation with BMP was injected onto a reverse-phase HPLC column (XTerra MS C18, 2.1 × 150 mm; Waters) equilibrated with 65% solvent A (0.05% TFA/0.05% formic acid) and 35% solvent B (0.05% TFA/0.05% formic acid in acetonitrile) at a flow rate of 0.3 ml/min. ..

Article Title: Investigation of host–pathogen interaction between Burkholderia pseudomallei and autophagy-related protein LC3 using hydrophobic chromatography-based technique
Article Snippet: Tryptic digested peptides were protonated with 0.1% formic acid before individually injecting into NanoAcquity system (Waters Corp., Milford, MA, USA) equipped with a Symmetry C18 5 μm, 180-μm × 20-mm Trap column and BEH130 C18 1.7 μm, and 100-μm × 100-mm analytical reversed phase column (Waters Corp., Milford, MA, USA). .. The reference sprayer of the NanoLockSpray source of mass spectrometer was [Glu1 ] fibrinopeptide B.

Article Title: A deep proteomics perspective on CRM1-mediated nuclear export and nucleocytoplasmic partitioning
Article Snippet: Paragraph title: Sample preparation for mass spectrometry ... Then, peptides were resuspended in 10 mM ammonium hydroxide (pH 10) and loaded onto a reverse phase HPLC column (XBridge C18, Waters, 3.5 µm, 1.0 mm x 150 mm) and eluted in a 5-35% (v/v) acetonitrile gradient at a flow rate of 60 µl/min.

Article Title: A molecular model of phosphorylation-based activation and potentiation of tarantula muscle thick filaments
Article Snippet: .. Tryptic digested peptides were extracted with 50% acetonitrile, 2% TFA in water and analyzed on an electro-spray quadrupole-time of flight Q-TOF liquid chromatography (LC) LC/MS/MS mass spectrometer with an in-line nanoflow LC (Micromass Q-Tof™ , Waters Corporation, USA). .. Resulting MS and MS/MS data were processed and viewed using MassLynx 4.0 software.

Article Title: A targeted metabolomics assay for cardiac metabolism and demonstration using a mouse model of dilated cardiomyopathy
Article Snippet: The strong mobile phase (A) was 10 mM ammonium acetate with 0.05 % ammonium hydroxide and the weak mobile phase was acetonitrile (B), and the LC column used was a BEH amide HILIC column (100 × 2.1 mm, 1.7 µm; Waters Ltd). .. The following linear gradient was used: 30 % A was held for 2 min followed by a linear gradient to 50 % A at 7 min with further re-equilibration for 3 min, the total run time was 10 min. Mass spectrometry parameters were the following: negative ion mode, a desolvation temperature of 300 °C, a source temperature of 110 °C, an ion spray voltage of 3.0 kV and a dwell time of 10 ms for each analyte.

BIA-KA:

Article Title: Modification of Tau by 8-Nitroguanosine 3′,5′-Cyclic Monophosphate (8-Nitro-cGMP)
Article Snippet: Tau was fractioned from the effluent by ammonium sulfate precipitation and loaded onto a reverse-phase HPLC column (COSMOSIL Protein-R Waters, Nacalai Tesque Inc.) following gel filtration chromatography to exchange buffers (NAP-10 column, GE Healthcare). .. Tau protein concentration was determined using a BCA protein assay kit (Pierce).

Modification:

Article Title: Pharmacokinetics of Cefepime in Patients with Thermal Burn Injury
Article Snippet: Cefepime serum and urine samples were analyzed by a modification of the method described by Barbhaiya et al. ( ). .. Chromatographic separation was performed with a reverse-phase HPLC column (Nova-Pak C-18; 3.9 by 150 mm; Waters).

Article Title: Novel pathophysiological markers are revealed by iTRAQ-based quantitative clinical proteomics approach in vascular dementia
Article Snippet: 2.4.2 In-gel tryptic digestion and isobaric labeling The samples (250 μg of proteins/condition) were then reduced (5 mM tris-(2-carboxyethyl) phosphine, 30 °C, 3 h) and alkylated (20 mM methyl methanethiosulfonate in isopropanol, room temperature, 45 min in dark) followed by a two-step digestion (16 h + 4 h) at 30 °C with sequencing-grade modified trypsin (Promega, Madison, WI, USA) at a ratio of 1:100 (trypsin: sample) in 50 mM triethylammonium bicarbonate (TEAB). .. The tryptic digested peptides were desalted using Sep-Pak C18 cartridges (Waters, Milford, MA, USA) and dried in a vacuum centrifuge.

Article Title: Modification of Tau by 8-Nitroguanosine 3′,5′-Cyclic Monophosphate (8-Nitro-cGMP)
Article Snippet: Wild-type three-repeat (2N3R) and four-repeat (2N4R) tau and their corresponding cysteine-to-alanine substituted mutants (C322A-2N3R, C291,322A-2N4R) were cloned into a pRK172 vector and purified by a modified method reported previously ( ). .. Tau was fractioned from the effluent by ammonium sulfate precipitation and loaded onto a reverse-phase HPLC column (COSMOSIL Protein-R Waters, Nacalai Tesque Inc.) following gel filtration chromatography to exchange buffers (NAP-10 column, GE Healthcare).

High Performance Liquid Chromatography:

Article Title: Pharmacokinetics of Cefepime in Patients with Thermal Burn Injury
Article Snippet: .. Chromatographic separation was performed with a reverse-phase HPLC column (Nova-Pak C-18; 3.9 by 150 mm; Waters). .. The mobile phase consisted of an 86% 0.0023 M 1-octanesulfonic acid sodium salt in HPLC-grade water and 14% (vol/vol) acetonitrile adjusted to pH 2.3 with 85% phosphoric acid.

Article Title: Oral curcumin for Alzheimer's disease: tolerability and efficacy in a 24-week randomized, double blind, placebo-controlled study
Article Snippet: .. Dried plasma/CSF extract samples were re-dissolved in acetonitrile:water (50/50, by volume) in 10 mM ammonium acetate, and an aliquot of the solution (typically 50 ul) was injected onto a reverse phase HPLC column (Atlantis T3, 150 × 2.1 mm; Waters Corporation, Milford, MA, USA). ..

Article Title: Mechanism-Based Inactivation of CYP2B1 and Its F-Helix Mutant by Two tert-Butyl Acetylenic Compounds: Covalent Modification of Prosthetic Heme Versus Apoprotein
Article Snippet: .. An aliquot (25 pmol) of the primary reaction mixtures inactivated by incubation with BMP was injected onto a reverse-phase HPLC column (XTerra MS C18, 2.1 × 150 mm; Waters) equilibrated with 65% solvent A (0.05% TFA/0.05% formic acid) and 35% solvent B (0.05% TFA/0.05% formic acid in acetonitrile) at a flow rate of 0.3 ml/min. ..

Article Title: Bioassay-Guided Isolation of Anti-Candida Biofilm Compounds From Methanol Extracts of the Aerial Parts of Salvia officinalis (Annaba, Algeria)
Article Snippet: .. The extracts were analyzed using a reverse-phase HPLC column: Sunfire™ prep C18 column (10 × 250 mm, 5 μm) (Waters, Ireland). .. The mobile phase was composed of solvent A (H2 O with 0.1% trifluoro acetic acid, Acros Organics) and solvent B (acetonitrile (ACN) with 0.1% TFA).

Article Title: Bioreactor culture of recombinant Drosophila melanogaster S2 cells: characterization of metabolic features related to cell growth and production of the rabies virus glycoprotein
Article Snippet: .. The concentrations of glucose, lactate and glutamine in the culture supernatants were determined enzymatically with a YSI Biochemical Analyzer (model 2700) after sample centrifugation at 200 g for 5 min. Amino acid concentrations in the culture supernatants were analyzed by the Pico-tag system, using a reverse-phase HPLC column (Waters Sweden, Sollentuna, Sweden) (Bidlingmeyer et al. ). ..

Article Title: A deep proteomics perspective on CRM1-mediated nuclear export and nucleocytoplasmic partitioning
Article Snippet: .. Then, peptides were resuspended in 10 mM ammonium hydroxide (pH 10) and loaded onto a reverse phase HPLC column (XBridge C18, Waters, 3.5 µm, 1.0 mm x 150 mm) and eluted in a 5-35% (v/v) acetonitrile gradient at a flow rate of 60 µl/min. ..

Article Title: A single amino acid substitution in a chitinase of the legume Medicago truncatula is sufficient to gain Nod-factor hydrolase activity
Article Snippet: .. Samples were taken up by 1 µl DMSO followed by 60 µl 50% acetonitrile and loaded on a reverse phase HPLC column (Nova Pak C18, 3.9 × 150 mm, particle size 4 mm; Waters). ..

Article Title: Modification of Tau by 8-Nitroguanosine 3′,5′-Cyclic Monophosphate (8-Nitro-cGMP)
Article Snippet: .. Tau was fractioned from the effluent by ammonium sulfate precipitation and loaded onto a reverse-phase HPLC column (COSMOSIL Protein-R Waters, Nacalai Tesque Inc.) following gel filtration chromatography to exchange buffers (NAP-10 column, GE Healthcare). .. Tau protein concentration was determined using a BCA protein assay kit (Pierce).

Article Title: A noninvasive analysis of urinary musculoskeletal collagen metabolism markers from rhesus monkeys subject to chronic hypergravity
Article Snippet: .. Processed acid hydrolysates containing the cross-link residues were filtered, dried, and redissolved in 0.01 M n -heptafluorobutyric acid (Pierce Chemicals) and loaded on a reverse-phase HPLC column (Sperisorb ODS2, Waters; 25 cm × 4.6 mm) equilibrated at 28°C. .. The collagen cross-links were eluted isocratically with 15% acetonitrile in 0.01 M heptafluorobutyric acid, monitored on a variable-wavelength fluorescence detector (model 470, Waters; 290-nm excitation and 395-nm emission), and compared with a known picomole amount of HP and LP standards (Metra Biosystems, Mountainview, CA).

Serum Assay:

Article Title: Pharmacokinetics of Cefepime in Patients with Thermal Burn Injury
Article Snippet: Paragraph title: Cefepime serum assay. ... Chromatographic separation was performed with a reverse-phase HPLC column (Nova-Pak C-18; 3.9 by 150 mm; Waters).

Chromatography:

Article Title: Oral curcumin for Alzheimer's disease: tolerability and efficacy in a 24-week randomized, double blind, placebo-controlled study
Article Snippet: Second, using liquid chromatography/tandem mass spectrometry (LC/MS/MS) plasma (baseline levels and 3 hour post-dose at the 24 week visit) and CSF levels (prior to the first dose and after the dose at the 24 week visit) of native curcumin were measured. .. Dried plasma/CSF extract samples were re-dissolved in acetonitrile:water (50/50, by volume) in 10 mM ammonium acetate, and an aliquot of the solution (typically 50 ul) was injected onto a reverse phase HPLC column (Atlantis T3, 150 × 2.1 mm; Waters Corporation, Milford, MA, USA).

Article Title: Mechanism-Based Inactivation of CYP2B1 and Its F-Helix Mutant by Two tert-Butyl Acetylenic Compounds: Covalent Modification of Prosthetic Heme Versus Apoprotein
Article Snippet: Paragraph title: Liquid Chromatography/Tandem Mass Spectrometry Analysis of Heme Adducts. ... An aliquot (25 pmol) of the primary reaction mixtures inactivated by incubation with BMP was injected onto a reverse-phase HPLC column (XTerra MS C18, 2.1 × 150 mm; Waters) equilibrated with 65% solvent A (0.05% TFA/0.05% formic acid) and 35% solvent B (0.05% TFA/0.05% formic acid in acetonitrile) at a flow rate of 0.3 ml/min.

Article Title: Bioassay-Guided Isolation of Anti-Candida Biofilm Compounds From Methanol Extracts of the Aerial Parts of Salvia officinalis (Annaba, Algeria)
Article Snippet: The chromatography data were acquired and processed using Lab Solution software. .. The extracts were analyzed using a reverse-phase HPLC column: Sunfire™ prep C18 column (10 × 250 mm, 5 μm) (Waters, Ireland).

Article Title: A molecular model of phosphorylation-based activation and potentiation of tarantula muscle thick filaments
Article Snippet: Paragraph title: Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis ... Tryptic digested peptides were extracted with 50% acetonitrile, 2% TFA in water and analyzed on an electro-spray quadrupole-time of flight Q-TOF liquid chromatography (LC) LC/MS/MS mass spectrometer with an in-line nanoflow LC (Micromass Q-Tof™ , Waters Corporation, USA).

Article Title: Modification of Tau by 8-Nitroguanosine 3′,5′-Cyclic Monophosphate (8-Nitro-cGMP)
Article Snippet: .. Tau was fractioned from the effluent by ammonium sulfate precipitation and loaded onto a reverse-phase HPLC column (COSMOSIL Protein-R Waters, Nacalai Tesque Inc.) following gel filtration chromatography to exchange buffers (NAP-10 column, GE Healthcare). .. Tau protein concentration was determined using a BCA protein assay kit (Pierce).

Article Title: A noninvasive analysis of urinary musculoskeletal collagen metabolism markers from rhesus monkeys subject to chronic hypergravity
Article Snippet: An aliquot of the hydrolysate was used for extraction of the HP and LP cross-links by CF1 cellulose (Whatman) partition chromatography as previously performed by our laboratory. .. Processed acid hydrolysates containing the cross-link residues were filtered, dried, and redissolved in 0.01 M n -heptafluorobutyric acid (Pierce Chemicals) and loaded on a reverse-phase HPLC column (Sperisorb ODS2, Waters; 25 cm × 4.6 mm) equilibrated at 28°C.

Article Title: A targeted metabolomics assay for cardiac metabolism and demonstration using a mouse model of dilated cardiomyopathy
Article Snippet: Analysis of HILIC mode polar compounds measured in negative ion mode including glycolytic intermediates and TCA cycle intermediates The sample from the positive ion mode analysis was recovered and analysed in a second UPLC chromatography assay. .. The strong mobile phase (A) was 10 mM ammonium acetate with 0.05 % ammonium hydroxide and the weak mobile phase was acetonitrile (B), and the LC column used was a BEH amide HILIC column (100 × 2.1 mm, 1.7 µm; Waters Ltd).

Protein Concentration:

Article Title: Modification of Tau by 8-Nitroguanosine 3′,5′-Cyclic Monophosphate (8-Nitro-cGMP)
Article Snippet: Tau was fractioned from the effluent by ammonium sulfate precipitation and loaded onto a reverse-phase HPLC column (COSMOSIL Protein-R Waters, Nacalai Tesque Inc.) following gel filtration chromatography to exchange buffers (NAP-10 column, GE Healthcare). .. Tau protein concentration was determined using a BCA protein assay kit (Pierce).

Sequencing:

Article Title: Novel pathophysiological markers are revealed by iTRAQ-based quantitative clinical proteomics approach in vascular dementia
Article Snippet: 2.4.2 In-gel tryptic digestion and isobaric labeling The samples (250 μg of proteins/condition) were then reduced (5 mM tris-(2-carboxyethyl) phosphine, 30 °C, 3 h) and alkylated (20 mM methyl methanethiosulfonate in isopropanol, room temperature, 45 min in dark) followed by a two-step digestion (16 h + 4 h) at 30 °C with sequencing-grade modified trypsin (Promega, Madison, WI, USA) at a ratio of 1:100 (trypsin: sample) in 50 mM triethylammonium bicarbonate (TEAB). .. The tryptic digested peptides were desalted using Sep-Pak C18 cartridges (Waters, Milford, MA, USA) and dried in a vacuum centrifuge.

Sonication:

Article Title: Modification of Tau by 8-Nitroguanosine 3′,5′-Cyclic Monophosphate (8-Nitro-cGMP)
Article Snippet: Cells were then sonicated and heated in boiling water to partially purify the heat-stable tau. .. Tau was fractioned from the effluent by ammonium sulfate precipitation and loaded onto a reverse-phase HPLC column (COSMOSIL Protein-R Waters, Nacalai Tesque Inc.) following gel filtration chromatography to exchange buffers (NAP-10 column, GE Healthcare).

Injection:

Article Title: Oral curcumin for Alzheimer's disease: tolerability and efficacy in a 24-week randomized, double blind, placebo-controlled study
Article Snippet: .. Dried plasma/CSF extract samples were re-dissolved in acetonitrile:water (50/50, by volume) in 10 mM ammonium acetate, and an aliquot of the solution (typically 50 ul) was injected onto a reverse phase HPLC column (Atlantis T3, 150 × 2.1 mm; Waters Corporation, Milford, MA, USA). ..

Article Title: Mechanism-Based Inactivation of CYP2B1 and Its F-Helix Mutant by Two tert-Butyl Acetylenic Compounds: Covalent Modification of Prosthetic Heme Versus Apoprotein
Article Snippet: .. An aliquot (25 pmol) of the primary reaction mixtures inactivated by incubation with BMP was injected onto a reverse-phase HPLC column (XTerra MS C18, 2.1 × 150 mm; Waters) equilibrated with 65% solvent A (0.05% TFA/0.05% formic acid) and 35% solvent B (0.05% TFA/0.05% formic acid in acetonitrile) at a flow rate of 0.3 ml/min. ..

Article Title: Bioassay-Guided Isolation of Anti-Candida Biofilm Compounds From Methanol Extracts of the Aerial Parts of Salvia officinalis (Annaba, Algeria)
Article Snippet: Plant samples were filtered through a CHROMAFIL®; Xtra H-PTFI filter (pore size 0.45 μm, filter 13 mm, MACHEREY-NAGEL, Germany) prior to HPLC injection. .. The extracts were analyzed using a reverse-phase HPLC column: Sunfire™ prep C18 column (10 × 250 mm, 5 μm) (Waters, Ireland).

Recombinant:

Article Title: Modification of Tau by 8-Nitroguanosine 3′,5′-Cyclic Monophosphate (8-Nitro-cGMP)
Article Snippet: Paragraph title: Purification of Recombinant Tau Isoforms ... Tau was fractioned from the effluent by ammonium sulfate precipitation and loaded onto a reverse-phase HPLC column (COSMOSIL Protein-R Waters, Nacalai Tesque Inc.) following gel filtration chromatography to exchange buffers (NAP-10 column, GE Healthcare).

Multiplexing:

Article Title: ProTargetMiner as a proteome signature library of anticancer molecules for functional discovery
Article Snippet: .. The deep proteomics samples (tags assigned in Supplementary Table ) were prepared according to the above protocol until the multiplexing, cleaning, and drying steps, after which the samples were resuspended in 20 mM ammonium hydroxide and separated into 96 fractions on an XBrigde BEH C18 2.1 × 150 mm column (Waters; Cat#186003023), using a Dionex Ultimate 3000 2DLC system (Thermo Scientific) over a 48 min gradient of 1–63%B (B = 20 mM ammonium hydroxide in acetonitrile) in three steps (1–23.5%B in 42 min, 23.5–54%B in 4 min and then 54–63%B in 2 min) at 200 µL min−1 flow. ..

Nucleic Acid Electrophoresis:

Article Title: A molecular model of phosphorylation-based activation and potentiation of tarantula muscle thick filaments
Article Snippet: These samples were analyzed by urea-glycerol gel electrophoresis. .. Tryptic digested peptides were extracted with 50% acetonitrile, 2% TFA in water and analyzed on an electro-spray quadrupole-time of flight Q-TOF liquid chromatography (LC) LC/MS/MS mass spectrometer with an in-line nanoflow LC (Micromass Q-Tof™ , Waters Corporation, USA).

Fluorescence:

Article Title: A noninvasive analysis of urinary musculoskeletal collagen metabolism markers from rhesus monkeys subject to chronic hypergravity
Article Snippet: Processed acid hydrolysates containing the cross-link residues were filtered, dried, and redissolved in 0.01 M n -heptafluorobutyric acid (Pierce Chemicals) and loaded on a reverse-phase HPLC column (Sperisorb ODS2, Waters; 25 cm × 4.6 mm) equilibrated at 28°C. .. The collagen cross-links were eluted isocratically with 15% acetonitrile in 0.01 M heptafluorobutyric acid, monitored on a variable-wavelength fluorescence detector (model 470, Waters; 290-nm excitation and 395-nm emission), and compared with a known picomole amount of HP and LP standards (Metra Biosystems, Mountainview, CA).

Mutagenesis:

Article Title: Modification of Tau by 8-Nitroguanosine 3′,5′-Cyclic Monophosphate (8-Nitro-cGMP)
Article Snippet: Briefly, wild-type and mutant tau in pRK172 vectors were expressed in Escherichia coli strain BL21 (DE3) cells (Takara Bio Inc.). .. Tau was fractioned from the effluent by ammonium sulfate precipitation and loaded onto a reverse-phase HPLC column (COSMOSIL Protein-R Waters, Nacalai Tesque Inc.) following gel filtration chromatography to exchange buffers (NAP-10 column, GE Healthcare).

Tandem Mass Spectroscopy:

Article Title: Mechanism-Based Inactivation of CYP2B1 and Its F-Helix Mutant by Two tert-Butyl Acetylenic Compounds: Covalent Modification of Prosthetic Heme Versus Apoprotein
Article Snippet: An aliquot (25 pmol) of the primary reaction mixtures inactivated by incubation with BMP was injected onto a reverse-phase HPLC column (XTerra MS C18, 2.1 × 150 mm; Waters) equilibrated with 65% solvent A (0.05% TFA/0.05% formic acid) and 35% solvent B (0.05% TFA/0.05% formic acid in acetonitrile) at a flow rate of 0.3 ml/min. .. Full-scan data were collected from m / z 300 to 2000, and MS/MS spectra were acquired in a data-dependent mode on the six most abundant ions in the survey scan.

Article Title: A molecular model of phosphorylation-based activation and potentiation of tarantula muscle thick filaments
Article Snippet: Tryptic digested peptides were extracted with 50% acetonitrile, 2% TFA in water and analyzed on an electro-spray quadrupole-time of flight Q-TOF liquid chromatography (LC) LC/MS/MS mass spectrometer with an in-line nanoflow LC (Micromass Q-Tof™ , Waters Corporation, USA). .. Resulting MS and MS/MS data were processed and viewed using MassLynx 4.0 software.

Flow Cytometry:

Article Title: Pharmacokinetics of Cefepime in Patients with Thermal Burn Injury
Article Snippet: Chromatographic separation was performed with a reverse-phase HPLC column (Nova-Pak C-18; 3.9 by 150 mm; Waters). .. The mobile-phase flow rate was 1 ml/min, the setting for full-scale absorbance units was 0.05, and the detection wavelength was 280 nm.

Article Title: Mechanism-Based Inactivation of CYP2B1 and Its F-Helix Mutant by Two tert-Butyl Acetylenic Compounds: Covalent Modification of Prosthetic Heme Versus Apoprotein
Article Snippet: .. An aliquot (25 pmol) of the primary reaction mixtures inactivated by incubation with BMP was injected onto a reverse-phase HPLC column (XTerra MS C18, 2.1 × 150 mm; Waters) equilibrated with 65% solvent A (0.05% TFA/0.05% formic acid) and 35% solvent B (0.05% TFA/0.05% formic acid in acetonitrile) at a flow rate of 0.3 ml/min. ..

Article Title: A deep proteomics perspective on CRM1-mediated nuclear export and nucleocytoplasmic partitioning
Article Snippet: .. Then, peptides were resuspended in 10 mM ammonium hydroxide (pH 10) and loaded onto a reverse phase HPLC column (XBridge C18, Waters, 3.5 µm, 1.0 mm x 150 mm) and eluted in a 5-35% (v/v) acetonitrile gradient at a flow rate of 60 µl/min. ..

Article Title: ProTargetMiner as a proteome signature library of anticancer molecules for functional discovery
Article Snippet: .. The deep proteomics samples (tags assigned in Supplementary Table ) were prepared according to the above protocol until the multiplexing, cleaning, and drying steps, after which the samples were resuspended in 20 mM ammonium hydroxide and separated into 96 fractions on an XBrigde BEH C18 2.1 × 150 mm column (Waters; Cat#186003023), using a Dionex Ultimate 3000 2DLC system (Thermo Scientific) over a 48 min gradient of 1–63%B (B = 20 mM ammonium hydroxide in acetonitrile) in three steps (1–23.5%B in 42 min, 23.5–54%B in 4 min and then 54–63%B in 2 min) at 200 µL min−1 flow. ..

Labeling:

Article Title: Novel pathophysiological markers are revealed by iTRAQ-based quantitative clinical proteomics approach in vascular dementia
Article Snippet: Paragraph title: In-gel tryptic digestion and isobaric labeling ... The tryptic digested peptides were desalted using Sep-Pak C18 cartridges (Waters, Milford, MA, USA) and dried in a vacuum centrifuge.

Purification:

Article Title: A single amino acid substitution in a chitinase of the legume Medicago truncatula is sufficient to gain Nod-factor hydrolase activity
Article Snippet: Enzyme assays with Nod-factors Defined amounts of purified proteins and NFs were incubated in 25 mM sodium acetate buffer (pH 5.0) at 37°C for an indicated time period. .. Samples were taken up by 1 µl DMSO followed by 60 µl 50% acetonitrile and loaded on a reverse phase HPLC column (Nova Pak C18, 3.9 × 150 mm, particle size 4 mm; Waters).

Article Title: Modification of Tau by 8-Nitroguanosine 3′,5′-Cyclic Monophosphate (8-Nitro-cGMP)
Article Snippet: Paragraph title: Purification of Recombinant Tau Isoforms ... Tau was fractioned from the effluent by ammonium sulfate precipitation and loaded onto a reverse-phase HPLC column (COSMOSIL Protein-R Waters, Nacalai Tesque Inc.) following gel filtration chromatography to exchange buffers (NAP-10 column, GE Healthcare).

Chloramphenicol Acetyltransferase Assay:

Article Title: ProTargetMiner as a proteome signature library of anticancer molecules for functional discovery
Article Snippet: .. The deep proteomics samples (tags assigned in Supplementary Table ) were prepared according to the above protocol until the multiplexing, cleaning, and drying steps, after which the samples were resuspended in 20 mM ammonium hydroxide and separated into 96 fractions on an XBrigde BEH C18 2.1 × 150 mm column (Waters; Cat186003023), using a Dionex Ultimate 3000 2DLC system (Thermo Scientific) over a 48 min gradient of 1–63%B (B = 20 mM ammonium hydroxide in acetonitrile) in three steps (1–23.5%B in 42 min, 23.5–54%B in 4 min and then 54–63%B in 2 min) at 200 µL min−1 flow. ..

Peptide Fractionation:

Article Title: ProTargetMiner as a proteome signature library of anticancer molecules for functional discovery
Article Snippet: Samples were then resuspended in 0.1% TFA, and separated into 8 fractions using High pH Reversed-Phase Peptide Fractionation Kit (Thermo; Cat#84868). .. The deep proteomics samples (tags assigned in Supplementary Table ) were prepared according to the above protocol until the multiplexing, cleaning, and drying steps, after which the samples were resuspended in 20 mM ammonium hydroxide and separated into 96 fractions on an XBrigde BEH C18 2.1 × 150 mm column (Waters; Cat#186003023), using a Dionex Ultimate 3000 2DLC system (Thermo Scientific) over a 48 min gradient of 1–63%B (B = 20 mM ammonium hydroxide in acetonitrile) in three steps (1–23.5%B in 42 min, 23.5–54%B in 4 min and then 54–63%B in 2 min) at 200 µL min−1 flow.

Liquid Chromatography:

Article Title: A molecular model of phosphorylation-based activation and potentiation of tarantula muscle thick filaments
Article Snippet: .. Tryptic digested peptides were extracted with 50% acetonitrile, 2% TFA in water and analyzed on an electro-spray quadrupole-time of flight Q-TOF liquid chromatography (LC) LC/MS/MS mass spectrometer with an in-line nanoflow LC (Micromass Q-Tof™ , Waters Corporation, USA). .. Resulting MS and MS/MS data were processed and viewed using MassLynx 4.0 software.

Plasmid Preparation:

Article Title: Modification of Tau by 8-Nitroguanosine 3′,5′-Cyclic Monophosphate (8-Nitro-cGMP)
Article Snippet: Wild-type three-repeat (2N3R) and four-repeat (2N4R) tau and their corresponding cysteine-to-alanine substituted mutants (C322A-2N3R, C291,322A-2N4R) were cloned into a pRK172 vector and purified by a modified method reported previously ( ). .. Tau was fractioned from the effluent by ammonium sulfate precipitation and loaded onto a reverse-phase HPLC column (COSMOSIL Protein-R Waters, Nacalai Tesque Inc.) following gel filtration chromatography to exchange buffers (NAP-10 column, GE Healthcare).

Software:

Article Title: Oral curcumin for Alzheimer's disease: tolerability and efficacy in a 24-week randomized, double blind, placebo-controlled study
Article Snippet: Dried plasma/CSF extract samples were re-dissolved in acetonitrile:water (50/50, by volume) in 10 mM ammonium acetate, and an aliquot of the solution (typically 50 ul) was injected onto a reverse phase HPLC column (Atlantis T3, 150 × 2.1 mm; Waters Corporation, Milford, MA, USA). .. Instrument manufacturer-supplied LC/MS/MS Varian software (Varian MS Workstation, Version 6.9.1) was used to calculate each ion according to its qualifier ion.

Article Title: Investigation of host–pathogen interaction between Burkholderia pseudomallei and autophagy-related protein LC3 using hydrophobic chromatography-based technique
Article Snippet: Tryptic digested peptides were protonated with 0.1% formic acid before individually injecting into NanoAcquity system (Waters Corp., Milford, MA, USA) equipped with a Symmetry C18 5 μm, 180-μm × 20-mm Trap column and BEH130 C18 1.7 μm, and 100-μm × 100-mm analytical reversed phase column (Waters Corp., Milford, MA, USA). .. All mass spectra were determined and compared among different conditions of protein solution obtained from the columns using DeCyder MS 2.0 Differential Analysis software (GE healthcare, USA) [ ].

Article Title: Bioassay-Guided Isolation of Anti-Candida Biofilm Compounds From Methanol Extracts of the Aerial Parts of Salvia officinalis (Annaba, Algeria)
Article Snippet: The chromatography data were acquired and processed using Lab Solution software. .. The extracts were analyzed using a reverse-phase HPLC column: Sunfire™ prep C18 column (10 × 250 mm, 5 μm) (Waters, Ireland).

Article Title: A molecular model of phosphorylation-based activation and potentiation of tarantula muscle thick filaments
Article Snippet: Tryptic digested peptides were extracted with 50% acetonitrile, 2% TFA in water and analyzed on an electro-spray quadrupole-time of flight Q-TOF liquid chromatography (LC) LC/MS/MS mass spectrometer with an in-line nanoflow LC (Micromass Q-Tof™ , Waters Corporation, USA). .. Resulting MS and MS/MS data were processed and viewed using MassLynx 4.0 software.

Hydrophilic Interaction Liquid Chromatography:

Article Title: A targeted metabolomics assay for cardiac metabolism and demonstration using a mouse model of dilated cardiomyopathy
Article Snippet: .. The strong mobile phase (A) was 10 mM ammonium acetate with 0.05 % ammonium hydroxide and the weak mobile phase was acetonitrile (B), and the LC column used was a BEH amide HILIC column (100 × 2.1 mm, 1.7 µm; Waters Ltd). .. The following linear gradient was used: 30 % A was held for 2 min followed by a linear gradient to 50 % A at 7 min with further re-equilibration for 3 min, the total run time was 10 min. Mass spectrometry parameters were the following: negative ion mode, a desolvation temperature of 300 °C, a source temperature of 110 °C, an ion spray voltage of 3.0 kV and a dwell time of 10 ms for each analyte.

Sample Prep:

Article Title: A deep proteomics perspective on CRM1-mediated nuclear export and nucleocytoplasmic partitioning
Article Snippet: Paragraph title: Sample preparation for mass spectrometry ... Then, peptides were resuspended in 10 mM ammonium hydroxide (pH 10) and loaded onto a reverse phase HPLC column (XBridge C18, Waters, 3.5 µm, 1.0 mm x 150 mm) and eluted in a 5-35% (v/v) acetonitrile gradient at a flow rate of 60 µl/min.

Concentration Assay:

Article Title: A deep proteomics perspective on CRM1-mediated nuclear export and nucleocytoplasmic partitioning
Article Snippet: Following digestion, the samples were acidified with trifluoroacetic acid (final concentration of 1%, v/v, 37°C, 1 h) to break down the RapiGest surfactant, the resulting by-products were pelleted by centrifugation (13,000 rpm, 15 min, RT), and the supernatant containing the digested peptides was transferred to a new tube. .. Then, peptides were resuspended in 10 mM ammonium hydroxide (pH 10) and loaded onto a reverse phase HPLC column (XBridge C18, Waters, 3.5 µm, 1.0 mm x 150 mm) and eluted in a 5-35% (v/v) acetonitrile gradient at a flow rate of 60 µl/min.

Fractionation:

Article Title: Novel pathophysiological markers are revealed by iTRAQ-based quantitative clinical proteomics approach in vascular dementia
Article Snippet: The tryptic digested peptides were desalted using Sep-Pak C18 cartridges (Waters, Milford, MA, USA) and dried in a vacuum centrifuge. .. The iTRAQ sample was desalted using Sep-Pak C18 cartridges and dried before fractionation.

Staining:

Article Title: A molecular model of phosphorylation-based activation and potentiation of tarantula muscle thick filaments
Article Snippet: Gels were handled with gloves in sterile conditions, and the Coomassie Blue G-250 stained RLC bands were excised and kept at −20°C. .. Tryptic digested peptides were extracted with 50% acetonitrile, 2% TFA in water and analyzed on an electro-spray quadrupole-time of flight Q-TOF liquid chromatography (LC) LC/MS/MS mass spectrometer with an in-line nanoflow LC (Micromass Q-Tof™ , Waters Corporation, USA).

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    Waters Corporation reversed phase c18 solid phase extraction cartridges
    Reversed Phase C18 Solid Phase Extraction Cartridges, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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