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TaKaRa restriction sites bamhi
Restriction Sites Bamhi, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/restriction sites bamhi/product/TaKaRa
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99
restriction sites bamhi - by Bioz Stars, 2020-07
88/100 stars

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Related Articles

Clone Assay:

Article Title: Molecular Determinants within N Terminus of Orai3 Protein That Control Channel Activation and Gating *
Article Snippet: .. Orai3 ΔN1–51 and Orai3 ΔN1–53 chimeras both containing the C terminus of Orai1 were cloned via splicing by overlap extension into pcDNA3.1V5 His TOPO vector (Invitrogen) and via the internal restriction sites BamHI and XbaI into the pEYFP-C1 (Clontech) expression vector for N-terminal fluorescence labeling. .. C-terminally enhanced GFP-labeled human CRACR2A and mouse CRACR2B were kindly provided by the lab of Yousang Gwack, University of California. cDNA was subcloned into pEYFP/pECFP-C1 vectors (Clontech) via their internal restriction sites BglII and EcoRI.

Fluorescence:

Article Title: Molecular Determinants within N Terminus of Orai3 Protein That Control Channel Activation and Gating *
Article Snippet: .. Orai3 ΔN1–51 and Orai3 ΔN1–53 chimeras both containing the C terminus of Orai1 were cloned via splicing by overlap extension into pcDNA3.1V5 His TOPO vector (Invitrogen) and via the internal restriction sites BamHI and XbaI into the pEYFP-C1 (Clontech) expression vector for N-terminal fluorescence labeling. .. C-terminally enhanced GFP-labeled human CRACR2A and mouse CRACR2B were kindly provided by the lab of Yousang Gwack, University of California. cDNA was subcloned into pEYFP/pECFP-C1 vectors (Clontech) via their internal restriction sites BglII and EcoRI.

Subcloning:

Article Title: Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization
Article Snippet: .. Plasmid peCFP-N1/Nef was generated by subcloning the PCR-amplified Nef coding region from pDsRed-N2/Nef into restriction sites BamHI and HindIII of peCFP-N1 (Clontech). .. Genes for GABARAP, GABARAPL1, GABARAPL2 and LC3B were subcloned from described GST-fusion plasmids by PCR amplification into the XhoI and BamHI sites of peYFP-C1 (Clontech), yielding peYFP-C1/GABARAP, peYFP-C1/GABARAPL1 and peYFP-C1/GABARAPL2.

Labeling:

Article Title: Molecular Determinants within N Terminus of Orai3 Protein That Control Channel Activation and Gating *
Article Snippet: .. Orai3 ΔN1–51 and Orai3 ΔN1–53 chimeras both containing the C terminus of Orai1 were cloned via splicing by overlap extension into pcDNA3.1V5 His TOPO vector (Invitrogen) and via the internal restriction sites BamHI and XbaI into the pEYFP-C1 (Clontech) expression vector for N-terminal fluorescence labeling. .. C-terminally enhanced GFP-labeled human CRACR2A and mouse CRACR2B were kindly provided by the lab of Yousang Gwack, University of California. cDNA was subcloned into pEYFP/pECFP-C1 vectors (Clontech) via their internal restriction sites BglII and EcoRI.

Generated:

Article Title: Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization
Article Snippet: .. Plasmid peCFP-N1/Nef was generated by subcloning the PCR-amplified Nef coding region from pDsRed-N2/Nef into restriction sites BamHI and HindIII of peCFP-N1 (Clontech). .. Genes for GABARAP, GABARAPL1, GABARAPL2 and LC3B were subcloned from described GST-fusion plasmids by PCR amplification into the XhoI and BamHI sites of peYFP-C1 (Clontech), yielding peYFP-C1/GABARAP, peYFP-C1/GABARAPL1 and peYFP-C1/GABARAPL2.

Overlap Extension Polymerase Chain Reaction:

Article Title: Molecular Determinants within N Terminus of Orai3 Protein That Control Channel Activation and Gating *
Article Snippet: .. Orai3 ΔN1–51 and Orai3 ΔN1–53 chimeras both containing the C terminus of Orai1 were cloned via splicing by overlap extension into pcDNA3.1V5 His TOPO vector (Invitrogen) and via the internal restriction sites BamHI and XbaI into the pEYFP-C1 (Clontech) expression vector for N-terminal fluorescence labeling. .. C-terminally enhanced GFP-labeled human CRACR2A and mouse CRACR2B were kindly provided by the lab of Yousang Gwack, University of California. cDNA was subcloned into pEYFP/pECFP-C1 vectors (Clontech) via their internal restriction sites BglII and EcoRI.

Expressing:

Article Title: Molecular Determinants within N Terminus of Orai3 Protein That Control Channel Activation and Gating *
Article Snippet: .. Orai3 ΔN1–51 and Orai3 ΔN1–53 chimeras both containing the C terminus of Orai1 were cloned via splicing by overlap extension into pcDNA3.1V5 His TOPO vector (Invitrogen) and via the internal restriction sites BamHI and XbaI into the pEYFP-C1 (Clontech) expression vector for N-terminal fluorescence labeling. .. C-terminally enhanced GFP-labeled human CRACR2A and mouse CRACR2B were kindly provided by the lab of Yousang Gwack, University of California. cDNA was subcloned into pEYFP/pECFP-C1 vectors (Clontech) via their internal restriction sites BglII and EcoRI.

Polymerase Chain Reaction:

Article Title: Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization
Article Snippet: .. Plasmid peCFP-N1/Nef was generated by subcloning the PCR-amplified Nef coding region from pDsRed-N2/Nef into restriction sites BamHI and HindIII of peCFP-N1 (Clontech). .. Genes for GABARAP, GABARAPL1, GABARAPL2 and LC3B were subcloned from described GST-fusion plasmids by PCR amplification into the XhoI and BamHI sites of peYFP-C1 (Clontech), yielding peYFP-C1/GABARAP, peYFP-C1/GABARAPL1 and peYFP-C1/GABARAPL2.

Plasmid Preparation:

Article Title: Molecular Determinants within N Terminus of Orai3 Protein That Control Channel Activation and Gating *
Article Snippet: .. Orai3 ΔN1–51 and Orai3 ΔN1–53 chimeras both containing the C terminus of Orai1 were cloned via splicing by overlap extension into pcDNA3.1V5 His TOPO vector (Invitrogen) and via the internal restriction sites BamHI and XbaI into the pEYFP-C1 (Clontech) expression vector for N-terminal fluorescence labeling. .. C-terminally enhanced GFP-labeled human CRACR2A and mouse CRACR2B were kindly provided by the lab of Yousang Gwack, University of California. cDNA was subcloned into pEYFP/pECFP-C1 vectors (Clontech) via their internal restriction sites BglII and EcoRI.

Article Title: Direct binding to GABARAP family members is essential for HIV-1 Nef plasma membrane localization
Article Snippet: .. Plasmid peCFP-N1/Nef was generated by subcloning the PCR-amplified Nef coding region from pDsRed-N2/Nef into restriction sites BamHI and HindIII of peCFP-N1 (Clontech). .. Genes for GABARAP, GABARAPL1, GABARAPL2 and LC3B were subcloned from described GST-fusion plasmids by PCR amplification into the XhoI and BamHI sites of peYFP-C1 (Clontech), yielding peYFP-C1/GABARAP, peYFP-C1/GABARAPL1 and peYFP-C1/GABARAPL2.

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    TaKaRa bamh1 restriction site
    Bamh1 Restriction Site, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamh1 restriction site/product/TaKaRa
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bamh1 restriction site - by Bioz Stars, 2020-07
    88/100 stars
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