restriction enzymes bamh i  (Thermo Fisher)


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    Structured Review

    Thermo Fisher restriction enzymes bamh i
    Recombinant plasmids of TgDPA. (A) Lane (1) the prokaryotic construct pET28/TgDPA was double digested by Bam I and Hind III enzymes and the product was resolved by 1% agarose gel to verify a band of size 807 bp. (M) Represents DNA Molecular marker. (B) Lanes 1 2, the <t>eukaryotic</t> construct pVAX1/TgDPA was double digested by <t>BamH</t> I and Xho I enzymes and the product was resolved by 1% agarose gel to verify a band of size 807 bp. (M) Represents DNA Molecular marker.
    Restriction Enzymes Bamh I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzymes bamh i/product/Thermo Fisher
    Average 88 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    restriction enzymes bamh i - by Bioz Stars, 2020-08
    88/100 stars

    Images

    1) Product Images from "DNA vaccination with a gene encoding Toxoplasma gondii Deoxyribose Phosphate Aldolase (TgDPA) induces partial protective immunity against lethal challenge in mice"

    Article Title: DNA vaccination with a gene encoding Toxoplasma gondii Deoxyribose Phosphate Aldolase (TgDPA) induces partial protective immunity against lethal challenge in mice

    Journal: Parasites & Vectors

    doi: 10.1186/1756-3305-7-431

    Recombinant plasmids of TgDPA. (A) Lane (1) the prokaryotic construct pET28/TgDPA was double digested by Bam I and Hind III enzymes and the product was resolved by 1% agarose gel to verify a band of size 807 bp. (M) Represents DNA Molecular marker. (B) Lanes 1 2, the eukaryotic construct pVAX1/TgDPA was double digested by BamH I and Xho I enzymes and the product was resolved by 1% agarose gel to verify a band of size 807 bp. (M) Represents DNA Molecular marker.
    Figure Legend Snippet: Recombinant plasmids of TgDPA. (A) Lane (1) the prokaryotic construct pET28/TgDPA was double digested by Bam I and Hind III enzymes and the product was resolved by 1% agarose gel to verify a band of size 807 bp. (M) Represents DNA Molecular marker. (B) Lanes 1 2, the eukaryotic construct pVAX1/TgDPA was double digested by BamH I and Xho I enzymes and the product was resolved by 1% agarose gel to verify a band of size 807 bp. (M) Represents DNA Molecular marker.

    Techniques Used: Recombinant, Construct, Agarose Gel Electrophoresis, Marker

    Related Articles

    Clone Assay:

    Article Title: Overexpression of Salusin-α Inhibits Vascular Intimal Hyperplasia in an Atherosclerotic Rabbit Model
    Article Snippet: .. The polymerase chain reaction (PCR) product of the salusin-α gene was digested with restriction enzymes BamH I and Xho I, and the desired fragment was cloned into the pcDNA3.1 expression plasmid (Invitrogen Life Technologies) to generate pcDNA3.1-salusin-α (pc-salusin-α ), which the salusin-α gene could be overexpressed by the pc-salusin-α plasmid transfected cells. .. Similar to our original study, the RNA interference psiHIV-U6-salusin-α (si-salusin-α ) recombinant was constructed as described using a Lenti-Pac lentiviral packaging kit (GeneCopoeia, MD, USA).

    Article Title: Overexpression of Salusin-α Inhibits Vascular Intimal Hyperplasia in an Atherosclerotic Rabbit Model
    Article Snippet: .. The polymerase chain reaction (PCR) product of the salusin- α gene was digested with restriction enzymes BamH I and Xho I, and the desired fragment was cloned into the pcDNA3.1 expression plasmid (Invitrogen Life Technologies) to generate pcDNA3.1-salusin- α (pc-salusin- α ), which the salusin- α gene could be overexpressed by the pc-salusin- α plasmid transfected cells. .. Similar to our original study, the RNA interference psiHIV-U6-salusin- α (si-salusin- α ) recombinant was constructed as described using a Lenti-Pac lentiviral packaging kit (GeneCopoeia, MD, USA).

    Transfection:

    Article Title: Overexpression of Salusin-α Inhibits Vascular Intimal Hyperplasia in an Atherosclerotic Rabbit Model
    Article Snippet: .. The polymerase chain reaction (PCR) product of the salusin-α gene was digested with restriction enzymes BamH I and Xho I, and the desired fragment was cloned into the pcDNA3.1 expression plasmid (Invitrogen Life Technologies) to generate pcDNA3.1-salusin-α (pc-salusin-α ), which the salusin-α gene could be overexpressed by the pc-salusin-α plasmid transfected cells. .. Similar to our original study, the RNA interference psiHIV-U6-salusin-α (si-salusin-α ) recombinant was constructed as described using a Lenti-Pac lentiviral packaging kit (GeneCopoeia, MD, USA).

    Article Title: Overexpression of Salusin-α Inhibits Vascular Intimal Hyperplasia in an Atherosclerotic Rabbit Model
    Article Snippet: .. The polymerase chain reaction (PCR) product of the salusin- α gene was digested with restriction enzymes BamH I and Xho I, and the desired fragment was cloned into the pcDNA3.1 expression plasmid (Invitrogen Life Technologies) to generate pcDNA3.1-salusin- α (pc-salusin- α ), which the salusin- α gene could be overexpressed by the pc-salusin- α plasmid transfected cells. .. Similar to our original study, the RNA interference psiHIV-U6-salusin- α (si-salusin- α ) recombinant was constructed as described using a Lenti-Pac lentiviral packaging kit (GeneCopoeia, MD, USA).

    Construct:

    Article Title: Anxa2 binds to STAT3 and promotes epithelial to mesenchymal transition in breast cancer cells
    Article Snippet: .. Plasmids expressing full-length and fragmental Anxa2 were built using PEGFP-N3 vector (Genechem Company), and the restriction enzymes BamH I and Xhol I (Thermo) were used. pCMV-C-Flag vector (Genechem company) was used to construct the plasmids expressing full-length STAT3 and two fragmental STAT3. .. The restriction enzymes HindIII and Sal I (Thermo) were also used.

    Article Title: Piperacillin-Tazobactam (TZP) Resistance in Escherichia coli Due to Hyperproduction of TEM-1 β-Lactamase Mediated by the Promoter Pa/Pb
    Article Snippet: .. In brief, plasmid DNAs of pRJ904 were extracted with the Plasmid DNA Mini Kit (Omega). pRJ904 and the clone vector pACYC184 were digested with restriction enzymes BamH I and Hind III (Thermo Fisher Scientific) and ligated to construct a DNA library, which was used to transform the competent cells. .. Selection was then performed with piperacillin (100 mg/L), tazobactam (4 mg/L), and chloramphenicol (50 mg/L).

    Expressing:

    Article Title: Anxa2 binds to STAT3 and promotes epithelial to mesenchymal transition in breast cancer cells
    Article Snippet: .. Plasmids expressing full-length and fragmental Anxa2 were built using PEGFP-N3 vector (Genechem Company), and the restriction enzymes BamH I and Xhol I (Thermo) were used. pCMV-C-Flag vector (Genechem company) was used to construct the plasmids expressing full-length STAT3 and two fragmental STAT3. .. The restriction enzymes HindIII and Sal I (Thermo) were also used.

    Article Title: Overexpression of Salusin-α Inhibits Vascular Intimal Hyperplasia in an Atherosclerotic Rabbit Model
    Article Snippet: .. The polymerase chain reaction (PCR) product of the salusin-α gene was digested with restriction enzymes BamH I and Xho I, and the desired fragment was cloned into the pcDNA3.1 expression plasmid (Invitrogen Life Technologies) to generate pcDNA3.1-salusin-α (pc-salusin-α ), which the salusin-α gene could be overexpressed by the pc-salusin-α plasmid transfected cells. .. Similar to our original study, the RNA interference psiHIV-U6-salusin-α (si-salusin-α ) recombinant was constructed as described using a Lenti-Pac lentiviral packaging kit (GeneCopoeia, MD, USA).

    Article Title: Overexpression of Salusin-α Inhibits Vascular Intimal Hyperplasia in an Atherosclerotic Rabbit Model
    Article Snippet: .. The polymerase chain reaction (PCR) product of the salusin- α gene was digested with restriction enzymes BamH I and Xho I, and the desired fragment was cloned into the pcDNA3.1 expression plasmid (Invitrogen Life Technologies) to generate pcDNA3.1-salusin- α (pc-salusin- α ), which the salusin- α gene could be overexpressed by the pc-salusin- α plasmid transfected cells. .. Similar to our original study, the RNA interference psiHIV-U6-salusin- α (si-salusin- α ) recombinant was constructed as described using a Lenti-Pac lentiviral packaging kit (GeneCopoeia, MD, USA).

    Polymerase Chain Reaction:

    Article Title: Overexpression of Salusin-α Inhibits Vascular Intimal Hyperplasia in an Atherosclerotic Rabbit Model
    Article Snippet: .. The polymerase chain reaction (PCR) product of the salusin-α gene was digested with restriction enzymes BamH I and Xho I, and the desired fragment was cloned into the pcDNA3.1 expression plasmid (Invitrogen Life Technologies) to generate pcDNA3.1-salusin-α (pc-salusin-α ), which the salusin-α gene could be overexpressed by the pc-salusin-α plasmid transfected cells. .. Similar to our original study, the RNA interference psiHIV-U6-salusin-α (si-salusin-α ) recombinant was constructed as described using a Lenti-Pac lentiviral packaging kit (GeneCopoeia, MD, USA).

    Article Title: Overexpression of Salusin-α Inhibits Vascular Intimal Hyperplasia in an Atherosclerotic Rabbit Model
    Article Snippet: .. The polymerase chain reaction (PCR) product of the salusin- α gene was digested with restriction enzymes BamH I and Xho I, and the desired fragment was cloned into the pcDNA3.1 expression plasmid (Invitrogen Life Technologies) to generate pcDNA3.1-salusin- α (pc-salusin- α ), which the salusin- α gene could be overexpressed by the pc-salusin- α plasmid transfected cells. .. Similar to our original study, the RNA interference psiHIV-U6-salusin- α (si-salusin- α ) recombinant was constructed as described using a Lenti-Pac lentiviral packaging kit (GeneCopoeia, MD, USA).

    Recombinant:

    Article Title: DNA vaccination with a gene encoding Toxoplasma gondii Deoxyribose Phosphate Aldolase (TgDPA) induces partial protective immunity against lethal challenge in mice
    Article Snippet: .. Construction of the eukaryotic plasmid The restriction enzymes BamH I and Xho I were used to digest the recombinant plasmid pET28a/TgDPA and the target gene DNA fragment was directionally sub-cloned into the pVAX1 vector (Invitrogen, Life Technologies), which was previously linearized with similar enzymes. .. The resultant recombinant plasmid designated pTgDPA was verified and confirmed by sequencing in both directions and also with double enzyme digestion.

    Plasmid Preparation:

    Article Title: Anxa2 binds to STAT3 and promotes epithelial to mesenchymal transition in breast cancer cells
    Article Snippet: .. Plasmids expressing full-length and fragmental Anxa2 were built using PEGFP-N3 vector (Genechem Company), and the restriction enzymes BamH I and Xhol I (Thermo) were used. pCMV-C-Flag vector (Genechem company) was used to construct the plasmids expressing full-length STAT3 and two fragmental STAT3. .. The restriction enzymes HindIII and Sal I (Thermo) were also used.

    Article Title: Piperacillin-Tazobactam (TZP) Resistance in Escherichia coli Due to Hyperproduction of TEM-1 β-Lactamase Mediated by the Promoter Pa/Pb
    Article Snippet: .. In brief, plasmid DNAs of pRJ904 were extracted with the Plasmid DNA Mini Kit (Omega). pRJ904 and the clone vector pACYC184 were digested with restriction enzymes BamH I and Hind III (Thermo Fisher Scientific) and ligated to construct a DNA library, which was used to transform the competent cells. .. Selection was then performed with piperacillin (100 mg/L), tazobactam (4 mg/L), and chloramphenicol (50 mg/L).

    Article Title: Overexpression of Salusin-α Inhibits Vascular Intimal Hyperplasia in an Atherosclerotic Rabbit Model
    Article Snippet: .. The polymerase chain reaction (PCR) product of the salusin-α gene was digested with restriction enzymes BamH I and Xho I, and the desired fragment was cloned into the pcDNA3.1 expression plasmid (Invitrogen Life Technologies) to generate pcDNA3.1-salusin-α (pc-salusin-α ), which the salusin-α gene could be overexpressed by the pc-salusin-α plasmid transfected cells. .. Similar to our original study, the RNA interference psiHIV-U6-salusin-α (si-salusin-α ) recombinant was constructed as described using a Lenti-Pac lentiviral packaging kit (GeneCopoeia, MD, USA).

    Article Title: Overexpression of Salusin-α Inhibits Vascular Intimal Hyperplasia in an Atherosclerotic Rabbit Model
    Article Snippet: .. The polymerase chain reaction (PCR) product of the salusin- α gene was digested with restriction enzymes BamH I and Xho I, and the desired fragment was cloned into the pcDNA3.1 expression plasmid (Invitrogen Life Technologies) to generate pcDNA3.1-salusin- α (pc-salusin- α ), which the salusin- α gene could be overexpressed by the pc-salusin- α plasmid transfected cells. .. Similar to our original study, the RNA interference psiHIV-U6-salusin- α (si-salusin- α ) recombinant was constructed as described using a Lenti-Pac lentiviral packaging kit (GeneCopoeia, MD, USA).

    Article Title: DNA vaccination with a gene encoding Toxoplasma gondii Deoxyribose Phosphate Aldolase (TgDPA) induces partial protective immunity against lethal challenge in mice
    Article Snippet: .. Construction of the eukaryotic plasmid The restriction enzymes BamH I and Xho I were used to digest the recombinant plasmid pET28a/TgDPA and the target gene DNA fragment was directionally sub-cloned into the pVAX1 vector (Invitrogen, Life Technologies), which was previously linearized with similar enzymes. .. The resultant recombinant plasmid designated pTgDPA was verified and confirmed by sequencing in both directions and also with double enzyme digestion.

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  • News
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  • 88
    Thermo Fisher bamh i restriction enzymes
    Bamh I Restriction Enzymes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamh i restriction enzymes/product/Thermo Fisher
    Average 88 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    bamh i restriction enzymes - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    90
    Thermo Fisher restriction enzyme bamh i
    Restriction Enzyme Bamh I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzyme bamh i/product/Thermo Fisher
    Average 90 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    restriction enzyme bamh i - by Bioz Stars, 2020-08
    90/100 stars
      Buy from Supplier

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