bamh i restriction enzymes  (Thermo Fisher)


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    Structured Review

    Thermo Fisher bamh i restriction enzymes
    Bamh I Restriction Enzymes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamh i restriction enzymes/product/Thermo Fisher
    Average 77 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    bamh i restriction enzymes - by Bioz Stars, 2020-01
    77/100 stars

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    Related Articles

    Clone Assay:

    Article Title: TCRP1 transcriptionally regulated by c-Myc confers cancer chemoresistance in tongue and lung cancer
    Article Snippet: The c-Myc fragment and pMxs-h vector (Promega, USA), and TCRP1 fragment and pLEX-MCS vector (Thermo Scientific, USA) were digested by restriction enzyme BamH I (Thermo scientific, USA) and Xho I (Thermo scientific, USA). .. We mutated c-Myc binding site “CAGTTG” to “TCATCT” within TCRP1 promoter following instructions of MutanBEST Kit (TaKaRa, China), and respectively cloned wild type (wt) and mutant TCRP1 promoter into a luciferase reporter vector pGL4, named pGL4-TCRP1-wt and pGL4-TCRP1-mut.

    Amplification:

    Article Title: TCRP1 transcriptionally regulated by c-Myc confers cancer chemoresistance in tongue and lung cancer
    Article Snippet: Construction plasmids Total RNA was isolated from Tca8113 cells and was inversely transcripted into cDNA, c-Myc ORF (open reading frame) and TCRP1 ORF were amplified by PrimeSTAR® HS DNA Polymerase (TaKaRa, Japan), and primers are showed in Table . .. The c-Myc fragment and pMxs-h vector (Promega, USA), and TCRP1 fragment and pLEX-MCS vector (Thermo Scientific, USA) were digested by restriction enzyme BamH I (Thermo scientific, USA) and Xho I (Thermo scientific, USA).

    Mutagenesis:

    Article Title: TCRP1 transcriptionally regulated by c-Myc confers cancer chemoresistance in tongue and lung cancer
    Article Snippet: The c-Myc fragment and pMxs-h vector (Promega, USA), and TCRP1 fragment and pLEX-MCS vector (Thermo Scientific, USA) were digested by restriction enzyme BamH I (Thermo scientific, USA) and Xho I (Thermo scientific, USA). .. We mutated c-Myc binding site “CAGTTG” to “TCATCT” within TCRP1 promoter following instructions of MutanBEST Kit (TaKaRa, China), and respectively cloned wild type (wt) and mutant TCRP1 promoter into a luciferase reporter vector pGL4, named pGL4-TCRP1-wt and pGL4-TCRP1-mut.

    Isolation:

    Article Title: TCRP1 transcriptionally regulated by c-Myc confers cancer chemoresistance in tongue and lung cancer
    Article Snippet: Construction plasmids Total RNA was isolated from Tca8113 cells and was inversely transcripted into cDNA, c-Myc ORF (open reading frame) and TCRP1 ORF were amplified by PrimeSTAR® HS DNA Polymerase (TaKaRa, Japan), and primers are showed in Table . .. The c-Myc fragment and pMxs-h vector (Promega, USA), and TCRP1 fragment and pLEX-MCS vector (Thermo Scientific, USA) were digested by restriction enzyme BamH I (Thermo scientific, USA) and Xho I (Thermo scientific, USA).

    Luciferase:

    Article Title: TCRP1 transcriptionally regulated by c-Myc confers cancer chemoresistance in tongue and lung cancer
    Article Snippet: The c-Myc fragment and pMxs-h vector (Promega, USA), and TCRP1 fragment and pLEX-MCS vector (Thermo Scientific, USA) were digested by restriction enzyme BamH I (Thermo scientific, USA) and Xho I (Thermo scientific, USA). .. We mutated c-Myc binding site “CAGTTG” to “TCATCT” within TCRP1 promoter following instructions of MutanBEST Kit (TaKaRa, China), and respectively cloned wild type (wt) and mutant TCRP1 promoter into a luciferase reporter vector pGL4, named pGL4-TCRP1-wt and pGL4-TCRP1-mut.

    Binding Assay:

    Article Title: TCRP1 transcriptionally regulated by c-Myc confers cancer chemoresistance in tongue and lung cancer
    Article Snippet: The c-Myc fragment and pMxs-h vector (Promega, USA), and TCRP1 fragment and pLEX-MCS vector (Thermo Scientific, USA) were digested by restriction enzyme BamH I (Thermo scientific, USA) and Xho I (Thermo scientific, USA). .. We mutated c-Myc binding site “CAGTTG” to “TCATCT” within TCRP1 promoter following instructions of MutanBEST Kit (TaKaRa, China), and respectively cloned wild type (wt) and mutant TCRP1 promoter into a luciferase reporter vector pGL4, named pGL4-TCRP1-wt and pGL4-TCRP1-mut.

    Plasmid Preparation:

    Article Title: TCRP1 transcriptionally regulated by c-Myc confers cancer chemoresistance in tongue and lung cancer
    Article Snippet: .. The c-Myc fragment and pMxs-h vector (Promega, USA), and TCRP1 fragment and pLEX-MCS vector (Thermo Scientific, USA) were digested by restriction enzyme BamH I (Thermo scientific, USA) and Xho I (Thermo scientific, USA). .. After digestion, the c-Myc fragment were ligased into pMxs-h vector and TCRP1 fragment were ligased into pLEX-MCS vector by T4 DNA ligase (Thermo scientific, USA), and then sequenced (Life Technologies, USA), named pMxs-h-c-Myc and pLEX-TCRP1, respectively.

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    Thermo Fisher bamh i restriction enzymes
    Bamh I Restriction Enzymes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamh i restriction enzymes/product/Thermo Fisher
    Average 77 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bamh i restriction enzymes - by Bioz Stars, 2020-01
    77/100 stars
      Buy from Supplier

    90
    Thermo Fisher restriction enzymes bamh i
    Restriction Enzymes Bamh I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzymes bamh i/product/Thermo Fisher
    Average 90 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    restriction enzymes bamh i - by Bioz Stars, 2020-01
    90/100 stars
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    87
    Thermo Fisher restriction enzyme bamh i
    Restriction Enzyme Bamh I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzyme bamh i/product/Thermo Fisher
    Average 87 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    restriction enzyme bamh i - by Bioz Stars, 2020-01
    87/100 stars
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