restriction enzyme hpych4 v  (New England Biolabs)


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    Structured Review

    New England Biolabs restriction enzyme hpych4 v
    Restriction Enzyme Hpych4 V, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 82/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzyme hpych4 v/product/New England Biolabs
    Average 82 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    restriction enzyme hpych4 v - by Bioz Stars, 2020-01
    82/100 stars

    Related Products / Commonly Used Together

    bsrgi
    mobii
    mlui

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Role of interleukin-21 and interleukin-21 receptor polymorphisms in the treatment of HBeAg-positive chronic hepatitis B patients with peginterferon
    Article Snippet: PCR was carried out in a volume of 20 μL containing 3.0 μL DNA, 1.0 μL of each primer, 12.5 μL 2∗Taq PCR MasterMix (TianGen Biotech Co. Ltd, Beijing, China) and 7.5 μL ddH2 O. PCR was carried out with initial denaturation for 3 minutes at 94°C, followed by 30 (IL-21 rs907715 and rs2221903) or 35 (IL-21R rs2285452 and rs3093301) cycles of denaturation for 30 seconds at 94°C, annealing for 30 seconds at 60°C (IL-21 rs2221903), 55°C (IL-21 rs907715), 56°C (IL-21R rs3093301 and rs2285452), extension for 30 seconds at 72°C, and a final extension for 10 minutes at 72°C. .. The products were digested with restriction enzyme HpyCH4 V, MobII, MluI, BsrGI (New England Biolabs) at 37°C for 2 hours, and analyzed on a 8% polyacrylamide gel electrophoresis.

    Amplification:

    Article Title: Role of interleukin-21 and interleukin-21 receptor polymorphisms in the treatment of HBeAg-positive chronic hepatitis B patients with peginterferon
    Article Snippet: A 236-bp PCR fragment including the IL-21 rs2221903 polymorphism was amplified using the primers: 5’-GGTACCTGGACACTGACGCCCA-3’ (upstream) and 5’-AAGGCAGTTTAGTGGCGACAGCC-3’ (downstream), a 222-bp PCR fragment including the IL-21 rs907715 polymorphism was amplified using the primers: 5’-CAATGGCTTGGTGTTTGGTAT-3’ (upstream), and 5’-CCTCTTTTCACTTGGA GCATTC-3’ (downstream), a 229-bp PCR fragment including the IL-21R rs2285452 polymorphism was amplified using the primers: 5’-CTGGGCTGTGATGTGA AGAC-3’ (upstream) and 5’-TGGCAGGTGATAAGGAACAA-3’ (downstream), and a 183-bp PCR fragment including the IL-21R rs3093301 polymorphism was amplified using the primers: 5’-CCCTCCCTCTTTCTTTGTTAG-3’ (upstream) and 5’-TCCTCCTACCTCGGCCTCTCAAAGTG-3’ (downstream). .. The products were digested with restriction enzyme HpyCH4 V, MobII, MluI, BsrGI (New England Biolabs) at 37°C for 2 hours, and analyzed on a 8% polyacrylamide gel electrophoresis.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Role of interleukin-21 and interleukin-21 receptor polymorphisms in the treatment of HBeAg-positive chronic hepatitis B patients with peginterferon
    Article Snippet: .. The products were digested with restriction enzyme HpyCH4 V, MobII, MluI, BsrGI (New England Biolabs) at 37°C for 2 hours, and analyzed on a 8% polyacrylamide gel electrophoresis. ..

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  • 82
    New England Biolabs restriction enzyme hpych4 v
    Restriction Enzyme Hpych4 V, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 82/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzyme hpych4 v/product/New England Biolabs
    Average 82 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    restriction enzyme hpych4 v - by Bioz Stars, 2020-01
    82/100 stars
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