Structured Review

TaKaRa restriction enzyme apali
Restriction Enzyme Apali, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/restriction enzyme apali/product/TaKaRa
Average 85 stars, based on 1 article reviews
Price from $9.99 to $1999.99
restriction enzyme apali - by Bioz Stars, 2020-08
85/100 stars

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Mutagenesis:

Article Title: Encephalomyocarditis Virus Disrupts Stress Granules, the Critical Platform for Triggering Antiviral Innate Immune Responses
Article Snippet: .. To generate HeLa cells stably expressing the EGFP-G3BP1 wild type (wt) and the EGFP-G3BP1 Q325E mutant, pEGFP-C1-G3BP1 and pEGFP-C1-G3BP1 Q325E mutant expression constructs were linearized by using the restriction enzyme ApaLI (TaKaRa, Japan). .. The linearized plasmids were then transfected into HeLa cells by using FuGENE6 (Promega, USA) according to manufacturer's recommendations.

Stable Transfection:

Article Title: Encephalomyocarditis Virus Disrupts Stress Granules, the Critical Platform for Triggering Antiviral Innate Immune Responses
Article Snippet: .. To generate HeLa cells stably expressing the EGFP-G3BP1 wild type (wt) and the EGFP-G3BP1 Q325E mutant, pEGFP-C1-G3BP1 and pEGFP-C1-G3BP1 Q325E mutant expression constructs were linearized by using the restriction enzyme ApaLI (TaKaRa, Japan). .. The linearized plasmids were then transfected into HeLa cells by using FuGENE6 (Promega, USA) according to manufacturer's recommendations.

Expressing:

Article Title: Encephalomyocarditis Virus Disrupts Stress Granules, the Critical Platform for Triggering Antiviral Innate Immune Responses
Article Snippet: .. To generate HeLa cells stably expressing the EGFP-G3BP1 wild type (wt) and the EGFP-G3BP1 Q325E mutant, pEGFP-C1-G3BP1 and pEGFP-C1-G3BP1 Q325E mutant expression constructs were linearized by using the restriction enzyme ApaLI (TaKaRa, Japan). .. The linearized plasmids were then transfected into HeLa cells by using FuGENE6 (Promega, USA) according to manufacturer's recommendations.

Construct:

Article Title: Encephalomyocarditis Virus Disrupts Stress Granules, the Critical Platform for Triggering Antiviral Innate Immune Responses
Article Snippet: .. To generate HeLa cells stably expressing the EGFP-G3BP1 wild type (wt) and the EGFP-G3BP1 Q325E mutant, pEGFP-C1-G3BP1 and pEGFP-C1-G3BP1 Q325E mutant expression constructs were linearized by using the restriction enzyme ApaLI (TaKaRa, Japan). .. The linearized plasmids were then transfected into HeLa cells by using FuGENE6 (Promega, USA) according to manufacturer's recommendations.

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    TaKaRa restriction enzyme apali
    Restriction Enzyme Apali, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzyme apali/product/TaKaRa
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    restriction enzyme apali - by Bioz Stars, 2020-08
    85/100 stars
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