restriction endonucleases eco ri  (New England Biolabs)


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    Structured Review

    New England Biolabs restriction endonucleases eco ri
    Restriction Endonucleases Eco Ri, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction endonucleases eco ri/product/New England Biolabs
    Average 88 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    restriction endonucleases eco ri - by Bioz Stars, 2020-08
    88/100 stars

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    Clone Assay:

    Article Title: Single-stranded DNA and RNA origami
    Article Snippet: .. Nicking endonuclease Nb.BbvCI (1000 units), restriction endonucleases Eco RI (5000 units), Xho I (5000 units) and Hind III (5000 units), T7 and T3 RNA polymerases (5000 units), PCR Cloning Kit (20 reactions), NEB 10-beta and NEB stable competent E. coli were purchased from New England Biolabs, Inc. pGEM-7zf (−) vector, Pure-yield plasmid miniprep system, and the Wizard SV Gel and PCR Clean-UP System were purchased from Promega. .. RNA Clean and Concentrator-25 was purchased from Zymo Research.

    Ligation:

    Article Title: Genetic Variability of the European Corn Borer, Ostrinia nubilalis, Suggests Gene Flow Between Populations in the Midwestern United States
    Article Snippet: .. Template preparation Approximately 160 ng of genomic DNA in 7 µ l of volume was incubated with restriction endonucleases Eco RI and Mse I (New England Biolabs, www.neb.com ) for 2.5 hrs at 37°C in a total volume of 12.5 µ l which contained 1.25 µ l of 10X One-Phor-All buffer (Amersham Pharmacia biotech, www.apbiotech.com ), 0.125 µ l of 10 U/µ lMse I enzyme (1.25U/reaction), 0.0625 µ l of 20 U/µ lEco RI enzyme (1.25 U/reaction), 0.125 µ l of 10 mg/ml BSA (bovine serum album) (New England Biolabs), and autoclaved nanopure water to make up the volume to 12.5 µ l. The restriction fragments were then incubated for 11.5 hrs at ∼ 25°C with 5 µ l ligation mixture containing 0.15 µ l of T4 DNA ligase, 0.5 µ l of 10X T4 ligase buffer (New England Biolabs), 0.5 µ l of 5 pmoles/µ lEco RI adapter, 0.5 µ l of 5 pmoles/µ l MseI adapter (Operon Technologies, www.operon.com ), and 3.35 µ l autoclaved nanopure water. .. Double stranded adapters for ligation to the corresponding cut made by the restriction enzyme were prepared by incubating equimolar amounts of both adapter strands for 10 min at 65°C, 10 min at 37°C, 10 min at 25°C and stored at -20°C.

    Purification:

    Article Title: Screening of a Fosmid Library of Marine Environmental Genomic DNA Fragments Reveals Four Clones Related to Members of the Order Planctomycetales
    Article Snippet: .. Purified fosmids were digested with the restriction endonucleases Eco RI and Hin dIII (New England Biolabs, Beverly, Mass.). ..

    Incubation:

    Article Title: Genetic Variability of the European Corn Borer, Ostrinia nubilalis, Suggests Gene Flow Between Populations in the Midwestern United States
    Article Snippet: .. Template preparation Approximately 160 ng of genomic DNA in 7 µ l of volume was incubated with restriction endonucleases Eco RI and Mse I (New England Biolabs, www.neb.com ) for 2.5 hrs at 37°C in a total volume of 12.5 µ l which contained 1.25 µ l of 10X One-Phor-All buffer (Amersham Pharmacia biotech, www.apbiotech.com ), 0.125 µ l of 10 U/µ lMse I enzyme (1.25U/reaction), 0.0625 µ l of 20 U/µ lEco RI enzyme (1.25 U/reaction), 0.125 µ l of 10 mg/ml BSA (bovine serum album) (New England Biolabs), and autoclaved nanopure water to make up the volume to 12.5 µ l. The restriction fragments were then incubated for 11.5 hrs at ∼ 25°C with 5 µ l ligation mixture containing 0.15 µ l of T4 DNA ligase, 0.5 µ l of 10X T4 ligase buffer (New England Biolabs), 0.5 µ l of 5 pmoles/µ lEco RI adapter, 0.5 µ l of 5 pmoles/µ l MseI adapter (Operon Technologies, www.operon.com ), and 3.35 µ l autoclaved nanopure water. .. Double stranded adapters for ligation to the corresponding cut made by the restriction enzyme were prepared by incubating equimolar amounts of both adapter strands for 10 min at 65°C, 10 min at 37°C, 10 min at 25°C and stored at -20°C.

    Polymerase Chain Reaction:

    Article Title: Single-stranded DNA and RNA origami
    Article Snippet: .. Nicking endonuclease Nb.BbvCI (1000 units), restriction endonucleases Eco RI (5000 units), Xho I (5000 units) and Hind III (5000 units), T7 and T3 RNA polymerases (5000 units), PCR Cloning Kit (20 reactions), NEB 10-beta and NEB stable competent E. coli were purchased from New England Biolabs, Inc. pGEM-7zf (−) vector, Pure-yield plasmid miniprep system, and the Wizard SV Gel and PCR Clean-UP System were purchased from Promega. .. RNA Clean and Concentrator-25 was purchased from Zymo Research.

    Plasmid Preparation:

    Article Title: Identification of New Delhi Metallo-?-lactamase 1 in Acinetobacter lwoffii of Food Animal Origin
    Article Snippet: .. Restriction endonucleases Eco RI, Xba I, and Bam HI (NEB, UK) were used to digest the plasmid DNA extracted from A. lwoffii SGC-HZ9 and transformant JM109-SGC-HZ9. ..

    Article Title: Mutational and Transcriptional Analyses of the Staphylococcus aureus Low Affinity Proline Transporter OpuD During in vitro Growth and Infection of Murine Tissues
    Article Snippet: .. To create the opuD∷lux reporter fusion, pXen5 plasmid DNA was extracted from using a commercial kit (Qiagen), digested using the restriction endonuclease Eco RI (NEB), cleaned using a QIAquick kit (Qiagen), blunted using the Klenow fragment of E. coli (NEB), and then digested again using Sst I (Invitrogen, Carlsbad, CA). .. The blunted/ Sst I pXen5 DNA was ligated to the 403 bp ProP3B/4 PCR product DNA that was cut with Sst I and used to transform E. coli DH5α-MCR cells (Gibco BRL) ( ).

    Article Title: Single-stranded DNA and RNA origami
    Article Snippet: .. Nicking endonuclease Nb.BbvCI (1000 units), restriction endonucleases Eco RI (5000 units), Xho I (5000 units) and Hind III (5000 units), T7 and T3 RNA polymerases (5000 units), PCR Cloning Kit (20 reactions), NEB 10-beta and NEB stable competent E. coli were purchased from New England Biolabs, Inc. pGEM-7zf (−) vector, Pure-yield plasmid miniprep system, and the Wizard SV Gel and PCR Clean-UP System were purchased from Promega. .. RNA Clean and Concentrator-25 was purchased from Zymo Research.

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    New England Biolabs restriction endonuclease ecori
    Restriction Endonuclease Ecori, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction endonuclease ecori/product/New England Biolabs
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    restriction endonuclease ecori - by Bioz Stars, 2020-08
    99/100 stars
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