reference protein glyceraldehyde 3 phosphate dehydrogenase  (Merck KGaA)

 
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    Structured Review

    Merck KGaA reference protein glyceraldehyde 3 phosphate dehydrogenase
    Reference Protein Glyceraldehyde 3 Phosphate Dehydrogenase, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reference protein glyceraldehyde 3 phosphate dehydrogenase/product/Merck KGaA
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    reference protein glyceraldehyde 3 phosphate dehydrogenase - by Bioz Stars, 2020-04
    96/100 stars

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    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: The Antidiabetic and Antinephritic Activities of Auricularia cornea (An Albino Mutant Strain) via Modulation of Oxidative Stress in the db/db Mice
    Article Snippet: Denatured protein samples (40 μg) were subjected to 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (Bio-Rad, USA) and then electro blotted onto 0.45 μm PVDF membranes (Bio Basic, Inc., USA). .. After blocking with 5% bovine serum albumin (BSA) for 4 h, the transferred membranes were incubated overnight at 4°C in the corresponding primary antibodies (at a dilution of 1:2,000) containing total-NF-κB (t-NF-κB, ab32536), phosphor-NF-κB (p-NF-κB, ab86299), Nrf2 (ab137550), catalase (CAT, ab16731), HO-1 (ab68477), HO-2 (ab90492), manganese superoxide dismutase 2 (SOD2, ab13533), protein kinase C alpha (PKC-α, ab23513), PKC β2 (ab32026), heat shock protein 27 (HSP27, ab12351), HSP 60 (ab45134), and HSP70 (ab181606) (Abcam, Cambridge, USA), and the reference protein glyceraldehyde-3-phosphate dehydrogenase (GAPDH; ABS16) (Merck Millipore, Darmstadt, Germany).

    Centrifugation:

    Article Title: The Antidiabetic and Antinephritic Activities of Auricularia cornea (An Albino Mutant Strain) via Modulation of Oxidative Stress in the db/db Mice
    Article Snippet: Western Blot One part of the kidney tissues obtained from the db/db mice was homogenized in RIPA buffer with a 1% protease inhibitor cocktail on ice for 30 min. After centrifugation (10,000 rpm for 10 min) and elimination of the precipitate, total protein concentrations were determined by BCA protein assay kit (Merck Millipore, USA). .. After blocking with 5% bovine serum albumin (BSA) for 4 h, the transferred membranes were incubated overnight at 4°C in the corresponding primary antibodies (at a dilution of 1:2,000) containing total-NF-κB (t-NF-κB, ab32536), phosphor-NF-κB (p-NF-κB, ab86299), Nrf2 (ab137550), catalase (CAT, ab16731), HO-1 (ab68477), HO-2 (ab90492), manganese superoxide dismutase 2 (SOD2, ab13533), protein kinase C alpha (PKC-α, ab23513), PKC β2 (ab32026), heat shock protein 27 (HSP27, ab12351), HSP 60 (ab45134), and HSP70 (ab181606) (Abcam, Cambridge, USA), and the reference protein glyceraldehyde-3-phosphate dehydrogenase (GAPDH; ABS16) (Merck Millipore, Darmstadt, Germany).

    Protease Inhibitor:

    Article Title: The Antidiabetic and Antinephritic Activities of Auricularia cornea (An Albino Mutant Strain) via Modulation of Oxidative Stress in the db/db Mice
    Article Snippet: Western Blot One part of the kidney tissues obtained from the db/db mice was homogenized in RIPA buffer with a 1% protease inhibitor cocktail on ice for 30 min. After centrifugation (10,000 rpm for 10 min) and elimination of the precipitate, total protein concentrations were determined by BCA protein assay kit (Merck Millipore, USA). .. After blocking with 5% bovine serum albumin (BSA) for 4 h, the transferred membranes were incubated overnight at 4°C in the corresponding primary antibodies (at a dilution of 1:2,000) containing total-NF-κB (t-NF-κB, ab32536), phosphor-NF-κB (p-NF-κB, ab86299), Nrf2 (ab137550), catalase (CAT, ab16731), HO-1 (ab68477), HO-2 (ab90492), manganese superoxide dismutase 2 (SOD2, ab13533), protein kinase C alpha (PKC-α, ab23513), PKC β2 (ab32026), heat shock protein 27 (HSP27, ab12351), HSP 60 (ab45134), and HSP70 (ab181606) (Abcam, Cambridge, USA), and the reference protein glyceraldehyde-3-phosphate dehydrogenase (GAPDH; ABS16) (Merck Millipore, Darmstadt, Germany).

    Blocking Assay:

    Article Title: The Antidiabetic and Antinephritic Activities of Auricularia cornea (An Albino Mutant Strain) via Modulation of Oxidative Stress in the db/db Mice
    Article Snippet: .. After blocking with 5% bovine serum albumin (BSA) for 4 h, the transferred membranes were incubated overnight at 4°C in the corresponding primary antibodies (at a dilution of 1:2,000) containing total-NF-κB (t-NF-κB, ab32536), phosphor-NF-κB (p-NF-κB, ab86299), Nrf2 (ab137550), catalase (CAT, ab16731), HO-1 (ab68477), HO-2 (ab90492), manganese superoxide dismutase 2 (SOD2, ab13533), protein kinase C alpha (PKC-α, ab23513), PKC β2 (ab32026), heat shock protein 27 (HSP27, ab12351), HSP 60 (ab45134), and HSP70 (ab181606) (Abcam, Cambridge, USA), and the reference protein glyceraldehyde-3-phosphate dehydrogenase (GAPDH; ABS16) (Merck Millipore, Darmstadt, Germany). .. The transferred membranes were washed five times with TBS buffer and then incubated with horseradish peroxidase-conjugated goat anti-rabbit secondary antibody (sc-3836) (Santa Cruz Biotechnology, Santa Cruz, USA) for 4 h at 4°C.

    Mouse Assay:

    Article Title: The Antidiabetic and Antinephritic Activities of Auricularia cornea (An Albino Mutant Strain) via Modulation of Oxidative Stress in the db/db Mice
    Article Snippet: Western Blot One part of the kidney tissues obtained from the db/db mice was homogenized in RIPA buffer with a 1% protease inhibitor cocktail on ice for 30 min. After centrifugation (10,000 rpm for 10 min) and elimination of the precipitate, total protein concentrations were determined by BCA protein assay kit (Merck Millipore, USA). .. After blocking with 5% bovine serum albumin (BSA) for 4 h, the transferred membranes were incubated overnight at 4°C in the corresponding primary antibodies (at a dilution of 1:2,000) containing total-NF-κB (t-NF-κB, ab32536), phosphor-NF-κB (p-NF-κB, ab86299), Nrf2 (ab137550), catalase (CAT, ab16731), HO-1 (ab68477), HO-2 (ab90492), manganese superoxide dismutase 2 (SOD2, ab13533), protein kinase C alpha (PKC-α, ab23513), PKC β2 (ab32026), heat shock protein 27 (HSP27, ab12351), HSP 60 (ab45134), and HSP70 (ab181606) (Abcam, Cambridge, USA), and the reference protein glyceraldehyde-3-phosphate dehydrogenase (GAPDH; ABS16) (Merck Millipore, Darmstadt, Germany).

    Incubation:

    Article Title: The Antidiabetic and Antinephritic Activities of Auricularia cornea (An Albino Mutant Strain) via Modulation of Oxidative Stress in the db/db Mice
    Article Snippet: .. After blocking with 5% bovine serum albumin (BSA) for 4 h, the transferred membranes were incubated overnight at 4°C in the corresponding primary antibodies (at a dilution of 1:2,000) containing total-NF-κB (t-NF-κB, ab32536), phosphor-NF-κB (p-NF-κB, ab86299), Nrf2 (ab137550), catalase (CAT, ab16731), HO-1 (ab68477), HO-2 (ab90492), manganese superoxide dismutase 2 (SOD2, ab13533), protein kinase C alpha (PKC-α, ab23513), PKC β2 (ab32026), heat shock protein 27 (HSP27, ab12351), HSP 60 (ab45134), and HSP70 (ab181606) (Abcam, Cambridge, USA), and the reference protein glyceraldehyde-3-phosphate dehydrogenase (GAPDH; ABS16) (Merck Millipore, Darmstadt, Germany). .. The transferred membranes were washed five times with TBS buffer and then incubated with horseradish peroxidase-conjugated goat anti-rabbit secondary antibody (sc-3836) (Santa Cruz Biotechnology, Santa Cruz, USA) for 4 h at 4°C.

    BIA-KA:

    Article Title: The Antidiabetic and Antinephritic Activities of Auricularia cornea (An Albino Mutant Strain) via Modulation of Oxidative Stress in the db/db Mice
    Article Snippet: Western Blot One part of the kidney tissues obtained from the db/db mice was homogenized in RIPA buffer with a 1% protease inhibitor cocktail on ice for 30 min. After centrifugation (10,000 rpm for 10 min) and elimination of the precipitate, total protein concentrations were determined by BCA protein assay kit (Merck Millipore, USA). .. After blocking with 5% bovine serum albumin (BSA) for 4 h, the transferred membranes were incubated overnight at 4°C in the corresponding primary antibodies (at a dilution of 1:2,000) containing total-NF-κB (t-NF-κB, ab32536), phosphor-NF-κB (p-NF-κB, ab86299), Nrf2 (ab137550), catalase (CAT, ab16731), HO-1 (ab68477), HO-2 (ab90492), manganese superoxide dismutase 2 (SOD2, ab13533), protein kinase C alpha (PKC-α, ab23513), PKC β2 (ab32026), heat shock protein 27 (HSP27, ab12351), HSP 60 (ab45134), and HSP70 (ab181606) (Abcam, Cambridge, USA), and the reference protein glyceraldehyde-3-phosphate dehydrogenase (GAPDH; ABS16) (Merck Millipore, Darmstadt, Germany).

    Western Blot:

    Article Title: The Antidiabetic and Antinephritic Activities of Auricularia cornea (An Albino Mutant Strain) via Modulation of Oxidative Stress in the db/db Mice
    Article Snippet: Paragraph title: Western Blot ... After blocking with 5% bovine serum albumin (BSA) for 4 h, the transferred membranes were incubated overnight at 4°C in the corresponding primary antibodies (at a dilution of 1:2,000) containing total-NF-κB (t-NF-κB, ab32536), phosphor-NF-κB (p-NF-κB, ab86299), Nrf2 (ab137550), catalase (CAT, ab16731), HO-1 (ab68477), HO-2 (ab90492), manganese superoxide dismutase 2 (SOD2, ab13533), protein kinase C alpha (PKC-α, ab23513), PKC β2 (ab32026), heat shock protein 27 (HSP27, ab12351), HSP 60 (ab45134), and HSP70 (ab181606) (Abcam, Cambridge, USA), and the reference protein glyceraldehyde-3-phosphate dehydrogenase (GAPDH; ABS16) (Merck Millipore, Darmstadt, Germany).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: The Antidiabetic and Antinephritic Activities of Auricularia cornea (An Albino Mutant Strain) via Modulation of Oxidative Stress in the db/db Mice
    Article Snippet: .. After blocking with 5% bovine serum albumin (BSA) for 4 h, the transferred membranes were incubated overnight at 4°C in the corresponding primary antibodies (at a dilution of 1:2,000) containing total-NF-κB (t-NF-κB, ab32536), phosphor-NF-κB (p-NF-κB, ab86299), Nrf2 (ab137550), catalase (CAT, ab16731), HO-1 (ab68477), HO-2 (ab90492), manganese superoxide dismutase 2 (SOD2, ab13533), protein kinase C alpha (PKC-α, ab23513), PKC β2 (ab32026), heat shock protein 27 (HSP27, ab12351), HSP 60 (ab45134), and HSP70 (ab181606) (Abcam, Cambridge, USA), and the reference protein glyceraldehyde-3-phosphate dehydrogenase (GAPDH; ABS16) (Merck Millipore, Darmstadt, Germany). .. The transferred membranes were washed five times with TBS buffer and then incubated with horseradish peroxidase-conjugated goat anti-rabbit secondary antibody (sc-3836) (Santa Cruz Biotechnology, Santa Cruz, USA) for 4 h at 4°C.

    SDS Page:

    Article Title: The Antidiabetic and Antinephritic Activities of Auricularia cornea (An Albino Mutant Strain) via Modulation of Oxidative Stress in the db/db Mice
    Article Snippet: Denatured protein samples (40 μg) were subjected to 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) (Bio-Rad, USA) and then electro blotted onto 0.45 μm PVDF membranes (Bio Basic, Inc., USA). .. After blocking with 5% bovine serum albumin (BSA) for 4 h, the transferred membranes were incubated overnight at 4°C in the corresponding primary antibodies (at a dilution of 1:2,000) containing total-NF-κB (t-NF-κB, ab32536), phosphor-NF-κB (p-NF-κB, ab86299), Nrf2 (ab137550), catalase (CAT, ab16731), HO-1 (ab68477), HO-2 (ab90492), manganese superoxide dismutase 2 (SOD2, ab13533), protein kinase C alpha (PKC-α, ab23513), PKC β2 (ab32026), heat shock protein 27 (HSP27, ab12351), HSP 60 (ab45134), and HSP70 (ab181606) (Abcam, Cambridge, USA), and the reference protein glyceraldehyde-3-phosphate dehydrogenase (GAPDH; ABS16) (Merck Millipore, Darmstadt, Germany).

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  • 96
    Merck KGaA reference protein glyceraldehyde 3 phosphate dehydrogenase
    Reference Protein Glyceraldehyde 3 Phosphate Dehydrogenase, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/reference protein glyceraldehyde 3 phosphate dehydrogenase/product/Merck KGaA
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    reference protein glyceraldehyde 3 phosphate dehydrogenase - by Bioz Stars, 2020-04
    96/100 stars
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