redtaq dna polymerase  (Millipore)


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    Structured Review

    Millipore redtaq dna polymerase
    Redtaq Dna Polymerase, supplied by Millipore, used in various techniques. Bioz Stars score: 87/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/redtaq dna polymerase/product/Millipore
    Average 87 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    redtaq dna polymerase - by Bioz Stars, 2020-04
    87/100 stars

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    Ligation:

    Article Title: Improving low-temperature activity of Sulfolobus acidocaldarius 2-keto-3-deoxygluconate aldolase
    Article Snippet: The 50-µl reaction mixtures contained 10 ng template DNA, 200 ng forward primer, 200 ng reverse primer, 0.2 mM dATP and dGTP, 1 mM dCTP and dTTP, 0.5 mM MnCl2 and 5U REDTaq DNA polymerase (Sigma) in REDTaq reaction buffer. .. The different ligation mixtures were transformed to E. coli XL-1 blue cells, plated and grown overnight.

    Mutagenesis:

    Article Title: Improving low-temperature activity of Sulfolobus acidocaldarius 2-keto-3-deoxygluconate aldolase
    Article Snippet: Random mutations were introduced by PCR using two DNA polymerases in parallel to generate a library with balanced mutation ratio. .. The 50-µl reaction mixtures contained 10 ng template DNA, 200 ng forward primer, 200 ng reverse primer, 0.2 mM dATP and dGTP, 1 mM dCTP and dTTP, 0.5 mM MnCl2 and 5U REDTaq DNA polymerase (Sigma) in REDTaq reaction buffer.

    Purification:

    Article Title: Improving low-temperature activity of Sulfolobus acidocaldarius 2-keto-3-deoxygluconate aldolase
    Article Snippet: The 50-µl reaction mixtures contained 10 ng template DNA, 200 ng forward primer, 200 ng reverse primer, 0.2 mM dATP and dGTP, 1 mM dCTP and dTTP, 0.5 mM MnCl2 and 5U REDTaq DNA polymerase (Sigma) in REDTaq reaction buffer. .. The PCR products obtained were purified, double digested with NcoI and SalI and ligated into equally digested pET24d with T4 DNA ligase.

    Polymerase Chain Reaction:

    Article Title: An investigation of dairy calf management practices, colostrum quality, failure of transfer of passive immunity, and occurrence of enteropathogens among Australian dairy farms.
    Article Snippet: .. The PCR mix was prepared with 5 µL of extracted DNA, 18 µL of 0.4 mM each primer, 0.2 mM each dNTP, 2.5 U of REDTaq DNA Polymerase (Sigma Aldrich, Castle Hill, NSW, Australia), and 5 µL of 10× REDTaq PCR Reaction buffer (containing (Izzo et al., 2011b) . ..

    Article Title: Improving low-temperature activity of Sulfolobus acidocaldarius 2-keto-3-deoxygluconate aldolase
    Article Snippet: Error-prone PCR with Taq DNA polymerase was performed with skewed nucleotide concentrations, with the following primers: 5′-ctttaagaaggagatataccatg-3′, and 5′-ggccgcaaccttgtcgactta-3′ and the pWUR193 plasmid as template DNA. .. The 50-µl reaction mixtures contained 10 ng template DNA, 200 ng forward primer, 200 ng reverse primer, 0.2 mM dATP and dGTP, 1 mM dCTP and dTTP, 0.5 mM MnCl2 and 5U REDTaq DNA polymerase (Sigma) in REDTaq reaction buffer.

    Incubation:

    Article Title: An investigation of dairy calf management practices, colostrum quality, failure of transfer of passive immunity, and occurrence of enteropathogens among Australian dairy farms.
    Article Snippet: Briefly, 10 g of feces were homogenized with 90 mL of sterile, buffered peptone water (Pail Buffered Peptone Water; Becton Dickinson and Company, Sparks, MD) and incubated for 18 ± 2 h at 37 ± 1°C for pre-enrichment. .. The PCR mix was prepared with 5 µL of extracted DNA, 18 µL of 0.4 mM each primer, 0.2 mM each dNTP, 2.5 U of REDTaq DNA Polymerase (Sigma Aldrich, Castle Hill, NSW, Australia), and 5 µL of 10× REDTaq PCR Reaction buffer (containing (Izzo et al., 2011b) .

    Sequencing:

    Article Title: Improving low-temperature activity of Sulfolobus acidocaldarius 2-keto-3-deoxygluconate aldolase
    Article Snippet: As such only the kdgA coding sequence was targeted in the ep-PCR procedure. .. The 50-µl reaction mixtures contained 10 ng template DNA, 200 ng forward primer, 200 ng reverse primer, 0.2 mM dATP and dGTP, 1 mM dCTP and dTTP, 0.5 mM MnCl2 and 5U REDTaq DNA polymerase (Sigma) in REDTaq reaction buffer.

    Transformation Assay:

    Article Title: Improving low-temperature activity of Sulfolobus acidocaldarius 2-keto-3-deoxygluconate aldolase
    Article Snippet: The 50-µl reaction mixtures contained 10 ng template DNA, 200 ng forward primer, 200 ng reverse primer, 0.2 mM dATP and dGTP, 1 mM dCTP and dTTP, 0.5 mM MnCl2 and 5U REDTaq DNA polymerase (Sigma) in REDTaq reaction buffer. .. The different ligation mixtures were transformed to E. coli XL-1 blue cells, plated and grown overnight.

    Plasmid Preparation:

    Article Title: Improving low-temperature activity of Sulfolobus acidocaldarius 2-keto-3-deoxygluconate aldolase
    Article Snippet: Error-prone PCR with Taq DNA polymerase was performed with skewed nucleotide concentrations, with the following primers: 5′-ctttaagaaggagatataccatg-3′, and 5′-ggccgcaaccttgtcgactta-3′ and the pWUR193 plasmid as template DNA. .. The 50-µl reaction mixtures contained 10 ng template DNA, 200 ng forward primer, 200 ng reverse primer, 0.2 mM dATP and dGTP, 1 mM dCTP and dTTP, 0.5 mM MnCl2 and 5U REDTaq DNA polymerase (Sigma) in REDTaq reaction buffer.

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    Millipore jumpstart redtaq readymix reaction mix
    Jumpstart Redtaq Readymix Reaction Mix, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/jumpstart redtaq readymix reaction mix/product/Millipore
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    jumpstart redtaq readymix reaction mix - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Millipore redtaq dna polymerase
    Redtaq Dna Polymerase, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/redtaq dna polymerase/product/Millipore
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    redtaq dna polymerase - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

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