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TopoGEN Inc recombinant top2a
Recombinant Top2a, supplied by TopoGEN Inc, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant top2a/product/TopoGEN Inc
Average 79 stars, based on 1 article reviews
Price from $9.99 to $1999.99
recombinant top2a - by Bioz Stars, 2020-01
79/100 stars

Related Products / Commonly Used Together

etoposide
top2 cleavage
pryg plasmid

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Related Articles

Magnetic Beads:

Article Title: Androgen-induced TOP2B mediated double strand breaks and prostate cancer gene rearrangements
Article Snippet: Biotin-labeled DNA was precipitated using Strepavidin coated magnetic beads (NEB), digested with NspI (NEB), and unbound fragments were removed by washing with wash Buffer (10 mM Tris HCl, 1mM EDTA, 2 M NaCl). .. To test the specificity of the SLOT assay, the pRYG plasmid, which contains a TOP2 cleavage and recognition site was incubated with recombinant TOP2A (TopoGen, Inc, Port Orange, FL) for 30 min at 37°C in the absence or presence of Etoposide (Sigma).

Labeling:

Article Title: Androgen-induced TOP2B mediated double strand breaks and prostate cancer gene rearrangements
Article Snippet: Tyrosine residues covalently linked to DNA were labeled using EDC and Amine-PEG3-Biotin (Thermo Scientific). .. To test the specificity of the SLOT assay, the pRYG plasmid, which contains a TOP2 cleavage and recognition site was incubated with recombinant TOP2A (TopoGen, Inc, Port Orange, FL) for 30 min at 37°C in the absence or presence of Etoposide (Sigma).

Purification:

Article Title: Androgen-induced TOP2B mediated double strand breaks and prostate cancer gene rearrangements
Article Snippet: Precipitated DNA was eluted in 95% formamide, 10 mM EDTA for 5 min at 65°C, purified using the Qiagen PCR purification Kit (Qiagen) and analyzed by real-time PCR. (See for an overview of the SLOT assay). .. To test the specificity of the SLOT assay, the pRYG plasmid, which contains a TOP2 cleavage and recognition site was incubated with recombinant TOP2A (TopoGen, Inc, Port Orange, FL) for 30 min at 37°C in the absence or presence of Etoposide (Sigma).

Real-time Polymerase Chain Reaction:

Article Title: Androgen-induced TOP2B mediated double strand breaks and prostate cancer gene rearrangements
Article Snippet: Precipitated DNA was eluted in 95% formamide, 10 mM EDTA for 5 min at 65°C, purified using the Qiagen PCR purification Kit (Qiagen) and analyzed by real-time PCR. (See for an overview of the SLOT assay). .. To test the specificity of the SLOT assay, the pRYG plasmid, which contains a TOP2 cleavage and recognition site was incubated with recombinant TOP2A (TopoGen, Inc, Port Orange, FL) for 30 min at 37°C in the absence or presence of Etoposide (Sigma).

Incubation:

Article Title: Androgen-induced TOP2B mediated double strand breaks and prostate cancer gene rearrangements
Article Snippet: .. To test the specificity of the SLOT assay, the pRYG plasmid, which contains a TOP2 cleavage and recognition site was incubated with recombinant TOP2A (TopoGen, Inc, Port Orange, FL) for 30 min at 37°C in the absence or presence of Etoposide (Sigma). .. Reactions were stopped by addition of SDS and were digested by proteinase K (Invitrogen).

Polymerase Chain Reaction:

Article Title: Androgen-induced TOP2B mediated double strand breaks and prostate cancer gene rearrangements
Article Snippet: Precipitated DNA was eluted in 95% formamide, 10 mM EDTA for 5 min at 65°C, purified using the Qiagen PCR purification Kit (Qiagen) and analyzed by real-time PCR. (See for an overview of the SLOT assay). .. To test the specificity of the SLOT assay, the pRYG plasmid, which contains a TOP2 cleavage and recognition site was incubated with recombinant TOP2A (TopoGen, Inc, Port Orange, FL) for 30 min at 37°C in the absence or presence of Etoposide (Sigma).

Lysis:

Article Title: Androgen-induced TOP2B mediated double strand breaks and prostate cancer gene rearrangements
Article Snippet: SLOT assay Cells were lysed in SLOT lysis buffer (2% SDS, 10 mM EDTA in 10 mM Tris-HCl pH 8) and DNA was extracted after proteinase K digestion using the Qiagen Blood and Tissue Kit (Qiagen). .. To test the specificity of the SLOT assay, the pRYG plasmid, which contains a TOP2 cleavage and recognition site was incubated with recombinant TOP2A (TopoGen, Inc, Port Orange, FL) for 30 min at 37°C in the absence or presence of Etoposide (Sigma).

Recombinant:

Article Title: Androgen-induced TOP2B mediated double strand breaks and prostate cancer gene rearrangements
Article Snippet: .. To test the specificity of the SLOT assay, the pRYG plasmid, which contains a TOP2 cleavage and recognition site was incubated with recombinant TOP2A (TopoGen, Inc, Port Orange, FL) for 30 min at 37°C in the absence or presence of Etoposide (Sigma). .. Reactions were stopped by addition of SDS and were digested by proteinase K (Invitrogen).

Plasmid Preparation:

Article Title: Androgen-induced TOP2B mediated double strand breaks and prostate cancer gene rearrangements
Article Snippet: .. To test the specificity of the SLOT assay, the pRYG plasmid, which contains a TOP2 cleavage and recognition site was incubated with recombinant TOP2A (TopoGen, Inc, Port Orange, FL) for 30 min at 37°C in the absence or presence of Etoposide (Sigma). .. Reactions were stopped by addition of SDS and were digested by proteinase K (Invitrogen).

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    TopoGEN Inc recombinant top2a
    Recombinant Top2a, supplied by TopoGEN Inc, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant top2a/product/TopoGEN Inc
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant top2a - by Bioz Stars, 2020-01
    79/100 stars
      Buy from Supplier

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