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Promega recombinant taq dna polymerase
Recombinant Taq Dna Polymerase, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant taq dna polymerase/product/Promega
Average 94 stars, based on 9 article reviews
Price from $9.99 to $1999.99
recombinant taq dna polymerase - by Bioz Stars, 2020-02
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Clone Assay:

Article Title: Broadcast Spawning Coral Mussismilia hispida Can Vertically Transfer its Associated Bacterial Core
Article Snippet: Symbiodinium Diversity The amplification of a specific region of the 28S rRNA gene of Symbiodinium was performed using the primers D1D2-F and D1D2-R ( ) in a solution containing 1X PCR buffer, 0.2 mM dNTPs, 2.5 mM MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega, Madison, WI, USA), 10–40 ng of total DNA, 0.5 μM of each primer and sterile Milli-Q water in a final volume of 15 μl. .. The reaction was performed in a thermocycler (Mastercycler Gradient, Eppendorf, Hamburg, Germany) with the following conditions: initial denaturing at 94°C for 5 min; 1 cycle at 94°C for 1 min, 56°C for 45 s and 72°C; 29 cycles at 92°C for 1 min, 56°C for 1 min and 72°C for 45 s; and a final extension at 72°C for 10 min. Polymerase chain reaction products were cloned using the pJET 1.2 vector (CloneJET PCR Cloning Kit #K1231, Thermo Scientific) and transformed into Escherichia coli competent cells (Single Step – KRX – Competent Cells, Promega) with the TransformAid Bacterial Transformation Kit (#K2710 Thermo Scientific) according to the manufacturer’s instructions.

Amplification:

Article Title: Broadcast Spawning Coral Mussismilia hispida Can Vertically Transfer its Associated Bacterial Core
Article Snippet: .. Bacterial Profile Specific regions of the gene encoding the 16S rRNA gene were amplified using the primers U968f GC and L1401r ( ) in a solution containing 1X PCR buffer, 0.2 mM dNTPs, 2.5 mM MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega, Madison, WI, USA), 10–40 ng of total DNA, 200 μmol of each primer and sterile Milli-Q water in a final volume of 50 μl. .. The reaction was performed in a thermocycler (Mastercycler Gradient, Eppendorf, Hamburg, Germany) with the following conditions: initial denaturing at 94°C for 3 min; 35 cycles at 94°C for 1 min, 55°C for 1 min and 72°C for 1 min; and a final extension at 72°C for 10 min.

Article Title: Comparison of DNA extraction protocols for microbial communities from soil treated with biochar
Article Snippet: .. The first amplification was performed in a solution containing 1X PCR buffer, 0.2 mM dNTPs, 3.75 mM MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega), 4 ng of total DNA, 200 μmol of each primer, 0.25 μL of bovine serum albumin (BSA) solution (20 mg mL−1 ) and sterile Milli-Q water in a final volume of 25 μL. .. The reaction was performed in a thermocycler (Mastercycler Gradient, Eppendorf, Hamburg, Germany).

Article Title: Overexpression of thioredoxin h leads to enhanced activity of starch debranching enzyme (pullulanase) in barley grain
Article Snippet: A 384-bp wtrxh coding region was amplified from pTaM13.38 , which contains a cDNA of wtrxh that served as a template in a PCR to create Xba I and Sac I sites by using the following primers: Wtrxh1 (5′-ata tctaga ATGGCGGCGTCGGCGGCGA -3′) and Wtrxh2R (5′-ata gagctc TTACTGGGCCGCGTGTAG -3′), (see below) (small, italicized letters in the primer denote a restriction enzyme site for subcloning of the DNA fragment containing wtrxh ; underlined letters denote wtrxh sequences). .. PCRs were performed on a thermocycler (MJ Research, Watertown, MA) by using 5 units of recombinant Taq DNA polymerase (Promega) in a 100-μl reaction volume.

Article Title: 18S rDNA Sequences from Microeukaryotes Reveal Oil Indicators in Mangrove Sediment
Article Snippet: .. The amplification was performed in a solution containing 1× PCR buffer, 0.2 mM dNTP, 2.0 mM MgCl2 , 0.75 U of recombinant Taq DNA polymerase (Promega), 10 ng of total DNA, 5 pmol of each primer and sterile Milli-Q water for a final volume of 25 µl. .. The reaction was performed in a thermocycler (Mastercycler Gradient, Eppendorf, Hamburg, Germany) under the following conditions: initial denaturation at 94°C for 30 s, 35 cycles at 94°C for 30 sec, 56°C for 45 sec and 72°C for 130 s with a final extension at 72°C for 7 min.

Article Title: Broadcast Spawning Coral Mussismilia hispida Can Vertically Transfer its Associated Bacterial Core
Article Snippet: .. Symbiodinium Diversity The amplification of a specific region of the 28S rRNA gene of Symbiodinium was performed using the primers D1D2-F and D1D2-R ( ) in a solution containing 1X PCR buffer, 0.2 mM dNTPs, 2.5 mM MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega, Madison, WI, USA), 10–40 ng of total DNA, 0.5 μM of each primer and sterile Milli-Q water in a final volume of 15 μl. .. The reaction was performed in a thermocycler (Mastercycler Gradient, Eppendorf, Hamburg, Germany) with the following conditions: initial denaturing at 94°C for 5 min; 1 cycle at 94°C for 1 min, 56°C for 45 s and 72°C; 29 cycles at 92°C for 1 min, 56°C for 1 min and 72°C for 45 s; and a final extension at 72°C for 10 min. Polymerase chain reaction products were cloned using the pJET 1.2 vector (CloneJET PCR Cloning Kit #K1231, Thermo Scientific) and transformed into Escherichia coli competent cells (Single Step – KRX – Competent Cells, Promega) with the TransformAid Bacterial Transformation Kit (#K2710 Thermo Scientific) according to the manufacturer’s instructions.

Article Title: Comparison of different protocols for the extraction of microbial DNA from reef corals
Article Snippet: .. The amplification was performed in a solution containing 1X PCR buffer, 0.2 mM of dNTP, 2.5 mM of MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega), 10 ng of total DNA, 200 μmol of each primer and sterile Milli-Q water for a final volume of 50 μL. .. The reaction was performed in a Mastercycler Gradient (Eppendorf, Hamburg, Germany) under the following conditions: initial denaturation at 94 °C for 3 min, 35 cycles at 94 °C for 1 min, 55 °C for 1 min and 72 °C for 1 min with a final extension at 72 °C for 10 min.

Article Title: Comparison of different protocols for the extraction of microbial DNA from reef corals
Article Snippet: .. The amplification was performed in a solution containing 1X PCR buffer, 0.2 mM of dNTP, 2.0 mM of MgCl2, 0.75 U of recombinant Taq DNA polymerase (Promega), 10 ng of total DNA, 5 pmol of each primer and sterile Milli-Q water for a final volume of 50 μl. .. The reaction was performed in a Mastercycler Gradient (Eppendorf, Hamburg, Germany) under the following conditions: initial denaturation at 94 °C for 30 sec, 35 cycles at 94 °C for 30 sec, 56 °C for 45 sec and 72 °C for 130 sec with a final extension at 72 °C for 7 min.

Article Title: Spatial Arrangement of Polycaprolactone/Collagen Nanofiber Scaffolds Regulates the Wound Healing Related Behaviors of Human Adipose Stromal Cells
Article Snippet: .. The isolated RNA was reverse transcribed into complementary DNA (cDNA) using the SuperScript First-Strand Synthesis System (Promega, Madison, WI), and the cDNA product was then amplified using recombinant Taq DNA polymerase (Promega). .. Expression of integrins α2 , α5 , αV , β1 , β3 , β6 , MMP-1, MMP-2, MMP-9, TGF-β1 , tropoelastin, type I collagen, type III collagen, vimentin, and α-SMA (ACTA-2) were determined over time. β-actin served as housekeeping gene.

Polymerase Chain Reaction:

Article Title: Broadcast Spawning Coral Mussismilia hispida Can Vertically Transfer its Associated Bacterial Core
Article Snippet: .. Bacterial Profile Specific regions of the gene encoding the 16S rRNA gene were amplified using the primers U968f GC and L1401r ( ) in a solution containing 1X PCR buffer, 0.2 mM dNTPs, 2.5 mM MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega, Madison, WI, USA), 10–40 ng of total DNA, 200 μmol of each primer and sterile Milli-Q water in a final volume of 50 μl. .. The reaction was performed in a thermocycler (Mastercycler Gradient, Eppendorf, Hamburg, Germany) with the following conditions: initial denaturing at 94°C for 3 min; 35 cycles at 94°C for 1 min, 55°C for 1 min and 72°C for 1 min; and a final extension at 72°C for 10 min.

Article Title: Comparison of DNA extraction protocols for microbial communities from soil treated with biochar
Article Snippet: .. The first amplification was performed in a solution containing 1X PCR buffer, 0.2 mM dNTPs, 3.75 mM MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega), 4 ng of total DNA, 200 μmol of each primer, 0.25 μL of bovine serum albumin (BSA) solution (20 mg mL−1 ) and sterile Milli-Q water in a final volume of 25 μL. .. The reaction was performed in a thermocycler (Mastercycler Gradient, Eppendorf, Hamburg, Germany).

Article Title: Overexpression of thioredoxin h leads to enhanced activity of starch debranching enzyme (pullulanase) in barley grain
Article Snippet: A 384-bp wtrxh coding region was amplified from pTaM13.38 , which contains a cDNA of wtrxh that served as a template in a PCR to create Xba I and Sac I sites by using the following primers: Wtrxh1 (5′-ata tctaga ATGGCGGCGTCGGCGGCGA -3′) and Wtrxh2R (5′-ata gagctc TTACTGGGCCGCGTGTAG -3′), (see below) (small, italicized letters in the primer denote a restriction enzyme site for subcloning of the DNA fragment containing wtrxh ; underlined letters denote wtrxh sequences). .. PCRs were performed on a thermocycler (MJ Research, Watertown, MA) by using 5 units of recombinant Taq DNA polymerase (Promega) in a 100-μl reaction volume.

Article Title: 18S rDNA Sequences from Microeukaryotes Reveal Oil Indicators in Mangrove Sediment
Article Snippet: .. The amplification was performed in a solution containing 1× PCR buffer, 0.2 mM dNTP, 2.0 mM MgCl2 , 0.75 U of recombinant Taq DNA polymerase (Promega), 10 ng of total DNA, 5 pmol of each primer and sterile Milli-Q water for a final volume of 25 µl. .. The reaction was performed in a thermocycler (Mastercycler Gradient, Eppendorf, Hamburg, Germany) under the following conditions: initial denaturation at 94°C for 30 s, 35 cycles at 94°C for 30 sec, 56°C for 45 sec and 72°C for 130 s with a final extension at 72°C for 7 min.

Article Title: Broadcast Spawning Coral Mussismilia hispida Can Vertically Transfer its Associated Bacterial Core
Article Snippet: .. Symbiodinium Diversity The amplification of a specific region of the 28S rRNA gene of Symbiodinium was performed using the primers D1D2-F and D1D2-R ( ) in a solution containing 1X PCR buffer, 0.2 mM dNTPs, 2.5 mM MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega, Madison, WI, USA), 10–40 ng of total DNA, 0.5 μM of each primer and sterile Milli-Q water in a final volume of 15 μl. .. The reaction was performed in a thermocycler (Mastercycler Gradient, Eppendorf, Hamburg, Germany) with the following conditions: initial denaturing at 94°C for 5 min; 1 cycle at 94°C for 1 min, 56°C for 45 s and 72°C; 29 cycles at 92°C for 1 min, 56°C for 1 min and 72°C for 45 s; and a final extension at 72°C for 10 min. Polymerase chain reaction products were cloned using the pJET 1.2 vector (CloneJET PCR Cloning Kit #K1231, Thermo Scientific) and transformed into Escherichia coli competent cells (Single Step – KRX – Competent Cells, Promega) with the TransformAid Bacterial Transformation Kit (#K2710 Thermo Scientific) according to the manufacturer’s instructions.

Article Title: A time frame permissive for Protein Kinase D2 activity to direct angiogenesis in mouse embryonic stem cells
Article Snippet: .. For PCR reaction, recombinant Taq DNA polymerase (Promega) was used. .. The reaction was performed in 25 μl containing 1 μl DNA, 0.25 μl Go-Taq 2.5 μl PCR buffer (10X), 2 μl 1.5 mM MgCl2 , 0.5 μl of 10 mM dNTP, 0.3 μl of primer.

Article Title: Comparison of different protocols for the extraction of microbial DNA from reef corals
Article Snippet: .. The amplification was performed in a solution containing 1X PCR buffer, 0.2 mM of dNTP, 2.5 mM of MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega), 10 ng of total DNA, 200 μmol of each primer and sterile Milli-Q water for a final volume of 50 μL. .. The reaction was performed in a Mastercycler Gradient (Eppendorf, Hamburg, Germany) under the following conditions: initial denaturation at 94 °C for 3 min, 35 cycles at 94 °C for 1 min, 55 °C for 1 min and 72 °C for 1 min with a final extension at 72 °C for 10 min.

Article Title: Comparison of different protocols for the extraction of microbial DNA from reef corals
Article Snippet: .. The amplification was performed in a solution containing 1X PCR buffer, 0.2 mM of dNTP, 2.0 mM of MgCl2, 0.75 U of recombinant Taq DNA polymerase (Promega), 10 ng of total DNA, 5 pmol of each primer and sterile Milli-Q water for a final volume of 50 μl. .. The reaction was performed in a Mastercycler Gradient (Eppendorf, Hamburg, Germany) under the following conditions: initial denaturation at 94 °C for 30 sec, 35 cycles at 94 °C for 30 sec, 56 °C for 45 sec and 72 °C for 130 sec with a final extension at 72 °C for 7 min.

Article Title: Spatial Arrangement of Polycaprolactone/Collagen Nanofiber Scaffolds Regulates the Wound Healing Related Behaviors of Human Adipose Stromal Cells
Article Snippet: Gene expression ( n =4 per group) was measured using reverse transcriptase polymerase chain reaction (RT-PCR) at 1, 3, and 7 days. .. The isolated RNA was reverse transcribed into complementary DNA (cDNA) using the SuperScript First-Strand Synthesis System (Promega, Madison, WI), and the cDNA product was then amplified using recombinant Taq DNA polymerase (Promega).

Electrophoresis:

Article Title: Broadcast Spawning Coral Mussismilia hispida Can Vertically Transfer its Associated Bacterial Core
Article Snippet: Bacterial Profile Specific regions of the gene encoding the 16S rRNA gene were amplified using the primers U968f GC and L1401r ( ) in a solution containing 1X PCR buffer, 0.2 mM dNTPs, 2.5 mM MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega, Madison, WI, USA), 10–40 ng of total DNA, 200 μmol of each primer and sterile Milli-Q water in a final volume of 50 μl. .. Electrophoresis was performed in Tris-acetate-EDTA buffer at 60°C at a constant voltage of 75 V for 16 h. The DGGE gels were stained with SYBR Green (Invitrogen, Carlsbad, CA, USA) and visualized using a Storm 860 Imaging System (GE Healthcare, Milwaukee, WI, USA).

Article Title: Comparison of DNA extraction protocols for microbial communities from soil treated with biochar
Article Snippet: Electrophoresis was performed in Tris-acetate-EDTA buffer at 60 °C at a constant voltage of 75 V for 16 hours. .. The first amplification was performed in a solution containing 1X PCR buffer, 0.2 mM dNTPs, 3.75 mM MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega), 4 ng of total DNA, 200 μmol of each primer, 0.25 μL of bovine serum albumin (BSA) solution (20 mg mL−1 ) and sterile Milli-Q water in a final volume of 25 μL.

Expressing:

Article Title: Overexpression of thioredoxin h leads to enhanced activity of starch debranching enzyme (pullulanase) in barley grain
Article Snippet: The ATG initiation codon for wtrxh expression was included in the Wtrxh1 primer. .. PCRs were performed on a thermocycler (MJ Research, Watertown, MA) by using 5 units of recombinant Taq DNA polymerase (Promega) in a 100-μl reaction volume.

Article Title: Spatial Arrangement of Polycaprolactone/Collagen Nanofiber Scaffolds Regulates the Wound Healing Related Behaviors of Human Adipose Stromal Cells
Article Snippet: Paragraph title: Gene expression ... The isolated RNA was reverse transcribed into complementary DNA (cDNA) using the SuperScript First-Strand Synthesis System (Promega, Madison, WI), and the cDNA product was then amplified using recombinant Taq DNA polymerase (Promega).

Transformation Assay:

Article Title: Broadcast Spawning Coral Mussismilia hispida Can Vertically Transfer its Associated Bacterial Core
Article Snippet: Symbiodinium Diversity The amplification of a specific region of the 28S rRNA gene of Symbiodinium was performed using the primers D1D2-F and D1D2-R ( ) in a solution containing 1X PCR buffer, 0.2 mM dNTPs, 2.5 mM MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega, Madison, WI, USA), 10–40 ng of total DNA, 0.5 μM of each primer and sterile Milli-Q water in a final volume of 15 μl. .. The reaction was performed in a thermocycler (Mastercycler Gradient, Eppendorf, Hamburg, Germany) with the following conditions: initial denaturing at 94°C for 5 min; 1 cycle at 94°C for 1 min, 56°C for 45 s and 72°C; 29 cycles at 92°C for 1 min, 56°C for 1 min and 72°C for 45 s; and a final extension at 72°C for 10 min. Polymerase chain reaction products were cloned using the pJET 1.2 vector (CloneJET PCR Cloning Kit #K1231, Thermo Scientific) and transformed into Escherichia coli competent cells (Single Step – KRX – Competent Cells, Promega) with the TransformAid Bacterial Transformation Kit (#K2710 Thermo Scientific) according to the manufacturer’s instructions.

Denaturing Gradient Gel Electrophoresis:

Article Title: Broadcast Spawning Coral Mussismilia hispida Can Vertically Transfer its Associated Bacterial Core
Article Snippet: Bacterial Profile Specific regions of the gene encoding the 16S rRNA gene were amplified using the primers U968f GC and L1401r ( ) in a solution containing 1X PCR buffer, 0.2 mM dNTPs, 2.5 mM MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega, Madison, WI, USA), 10–40 ng of total DNA, 200 μmol of each primer and sterile Milli-Q water in a final volume of 50 μl. .. The denaturing gradient gel elecrtophoresis (DGGE) gels (45–65% urea and formamide) were prepared with a solution of polyacrylamide (6%) in Tris-acetate (pH 8.3).

Article Title: Comparison of DNA extraction protocols for microbial communities from soil treated with biochar
Article Snippet: The DGGE gels were stained with SYBR Green (Molecular Probes) and visualised using a Storm 860 Imaging System (GE Healthcare). .. The first amplification was performed in a solution containing 1X PCR buffer, 0.2 mM dNTPs, 3.75 mM MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega), 4 ng of total DNA, 200 μmol of each primer, 0.25 μL of bovine serum albumin (BSA) solution (20 mg mL−1 ) and sterile Milli-Q water in a final volume of 25 μL.

Article Title: 18S rDNA Sequences from Microeukaryotes Reveal Oil Indicators in Mangrove Sediment
Article Snippet: The amplification was performed in a solution containing 1× PCR buffer, 0.2 mM dNTP, 2.0 mM MgCl2 , 0.75 U of recombinant Taq DNA polymerase (Promega), 10 ng of total DNA, 5 pmol of each primer and sterile Milli-Q water for a final volume of 25 µl. .. The amplicons were then separated by denaturing gradient gel electrophoresis (DGGE).

Article Title: Comparison of different protocols for the extraction of microbial DNA from reef corals
Article Snippet: The amplification was performed in a solution containing 1X PCR buffer, 0.2 mM of dNTP, 2.5 mM of MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega), 10 ng of total DNA, 200 μmol of each primer and sterile Milli-Q water for a final volume of 50 μL. .. The DGGE gels (30 to 65% of urea and formamide) were prepared with a solution of polyacrylamide (6%) in Tris-acetate (pH 8.3).

Article Title: Comparison of different protocols for the extraction of microbial DNA from reef corals
Article Snippet: The DGGE gels were stained with Sybr Green (Molecular Probes) and visualized using a Storm 860 Imaging System (GE Healthcare). .. The amplification was performed in a solution containing 1X PCR buffer, 0.2 mM of dNTP, 2.0 mM of MgCl2, 0.75 U of recombinant Taq DNA polymerase (Promega), 10 ng of total DNA, 5 pmol of each primer and sterile Milli-Q water for a final volume of 50 μl.

Article Title: Comparison of DNA extraction protocols for microbial communities from soil treated with biochar
Article Snippet: The amplification was performed in a solution containing 1X PCR buffer, 0.2 mM dNTPs, 2.5 mM MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega), 4 ng of total DNA, 200 μmol of each primer and sterile Milli-Q water in a final volume of 50 μL. .. The DGGE gels (45–65% urea and formamide) were prepared with a solution of polyacrylamide (6%) in Tris-acetate (pH 8.3).

Reverse Transcription Polymerase Chain Reaction:

Article Title: Spatial Arrangement of Polycaprolactone/Collagen Nanofiber Scaffolds Regulates the Wound Healing Related Behaviors of Human Adipose Stromal Cells
Article Snippet: Gene expression ( n =4 per group) was measured using reverse transcriptase polymerase chain reaction (RT-PCR) at 1, 3, and 7 days. .. The isolated RNA was reverse transcribed into complementary DNA (cDNA) using the SuperScript First-Strand Synthesis System (Promega, Madison, WI), and the cDNA product was then amplified using recombinant Taq DNA polymerase (Promega).

Imaging:

Article Title: Broadcast Spawning Coral Mussismilia hispida Can Vertically Transfer its Associated Bacterial Core
Article Snippet: Bacterial Profile Specific regions of the gene encoding the 16S rRNA gene were amplified using the primers U968f GC and L1401r ( ) in a solution containing 1X PCR buffer, 0.2 mM dNTPs, 2.5 mM MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega, Madison, WI, USA), 10–40 ng of total DNA, 200 μmol of each primer and sterile Milli-Q water in a final volume of 50 μl. .. Electrophoresis was performed in Tris-acetate-EDTA buffer at 60°C at a constant voltage of 75 V for 16 h. The DGGE gels were stained with SYBR Green (Invitrogen, Carlsbad, CA, USA) and visualized using a Storm 860 Imaging System (GE Healthcare, Milwaukee, WI, USA).

Article Title: Comparison of DNA extraction protocols for microbial communities from soil treated with biochar
Article Snippet: The DGGE gels were stained with SYBR Green (Molecular Probes) and visualised using a Storm 860 Imaging System (GE Healthcare). .. The first amplification was performed in a solution containing 1X PCR buffer, 0.2 mM dNTPs, 3.75 mM MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega), 4 ng of total DNA, 200 μmol of each primer, 0.25 μL of bovine serum albumin (BSA) solution (20 mg mL−1 ) and sterile Milli-Q water in a final volume of 25 μL.

Article Title: Comparison of different protocols for the extraction of microbial DNA from reef corals
Article Snippet: The DGGE gels were stained with Sybr Green (Molecular Probes) and visualized using a Storm 860 Imaging System (GE Healthcare). .. The amplification was performed in a solution containing 1X PCR buffer, 0.2 mM of dNTP, 2.0 mM of MgCl2, 0.75 U of recombinant Taq DNA polymerase (Promega), 10 ng of total DNA, 5 pmol of each primer and sterile Milli-Q water for a final volume of 50 μl.

Sequencing:

Article Title: Broadcast Spawning Coral Mussismilia hispida Can Vertically Transfer its Associated Bacterial Core
Article Snippet: Symbiodinium Diversity The amplification of a specific region of the 28S rRNA gene of Symbiodinium was performed using the primers D1D2-F and D1D2-R ( ) in a solution containing 1X PCR buffer, 0.2 mM dNTPs, 2.5 mM MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega, Madison, WI, USA), 10–40 ng of total DNA, 0.5 μM of each primer and sterile Milli-Q water in a final volume of 15 μl. .. The RFLPs enabled the selection of distinct Symbiodinium lineages for sequencing.

Recombinant:

Article Title: Broadcast Spawning Coral Mussismilia hispida Can Vertically Transfer its Associated Bacterial Core
Article Snippet: .. Bacterial Profile Specific regions of the gene encoding the 16S rRNA gene were amplified using the primers U968f GC and L1401r ( ) in a solution containing 1X PCR buffer, 0.2 mM dNTPs, 2.5 mM MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega, Madison, WI, USA), 10–40 ng of total DNA, 200 μmol of each primer and sterile Milli-Q water in a final volume of 50 μl. .. The reaction was performed in a thermocycler (Mastercycler Gradient, Eppendorf, Hamburg, Germany) with the following conditions: initial denaturing at 94°C for 3 min; 35 cycles at 94°C for 1 min, 55°C for 1 min and 72°C for 1 min; and a final extension at 72°C for 10 min.

Article Title: Comparison of DNA extraction protocols for microbial communities from soil treated with biochar
Article Snippet: .. The first amplification was performed in a solution containing 1X PCR buffer, 0.2 mM dNTPs, 3.75 mM MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega), 4 ng of total DNA, 200 μmol of each primer, 0.25 μL of bovine serum albumin (BSA) solution (20 mg mL−1 ) and sterile Milli-Q water in a final volume of 25 μL. .. The reaction was performed in a thermocycler (Mastercycler Gradient, Eppendorf, Hamburg, Germany).

Article Title: Overexpression of thioredoxin h leads to enhanced activity of starch debranching enzyme (pullulanase) in barley grain
Article Snippet: .. PCRs were performed on a thermocycler (MJ Research, Watertown, MA) by using 5 units of recombinant Taq DNA polymerase (Promega) in a 100-μl reaction volume. ..

Article Title: 18S rDNA Sequences from Microeukaryotes Reveal Oil Indicators in Mangrove Sediment
Article Snippet: .. The amplification was performed in a solution containing 1× PCR buffer, 0.2 mM dNTP, 2.0 mM MgCl2 , 0.75 U of recombinant Taq DNA polymerase (Promega), 10 ng of total DNA, 5 pmol of each primer and sterile Milli-Q water for a final volume of 25 µl. .. The reaction was performed in a thermocycler (Mastercycler Gradient, Eppendorf, Hamburg, Germany) under the following conditions: initial denaturation at 94°C for 30 s, 35 cycles at 94°C for 30 sec, 56°C for 45 sec and 72°C for 130 s with a final extension at 72°C for 7 min.

Article Title: Broadcast Spawning Coral Mussismilia hispida Can Vertically Transfer its Associated Bacterial Core
Article Snippet: .. Symbiodinium Diversity The amplification of a specific region of the 28S rRNA gene of Symbiodinium was performed using the primers D1D2-F and D1D2-R ( ) in a solution containing 1X PCR buffer, 0.2 mM dNTPs, 2.5 mM MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega, Madison, WI, USA), 10–40 ng of total DNA, 0.5 μM of each primer and sterile Milli-Q water in a final volume of 15 μl. .. The reaction was performed in a thermocycler (Mastercycler Gradient, Eppendorf, Hamburg, Germany) with the following conditions: initial denaturing at 94°C for 5 min; 1 cycle at 94°C for 1 min, 56°C for 45 s and 72°C; 29 cycles at 92°C for 1 min, 56°C for 1 min and 72°C for 45 s; and a final extension at 72°C for 10 min. Polymerase chain reaction products were cloned using the pJET 1.2 vector (CloneJET PCR Cloning Kit #K1231, Thermo Scientific) and transformed into Escherichia coli competent cells (Single Step – KRX – Competent Cells, Promega) with the TransformAid Bacterial Transformation Kit (#K2710 Thermo Scientific) according to the manufacturer’s instructions.

Article Title: A time frame permissive for Protein Kinase D2 activity to direct angiogenesis in mouse embryonic stem cells
Article Snippet: .. For PCR reaction, recombinant Taq DNA polymerase (Promega) was used. .. The reaction was performed in 25 μl containing 1 μl DNA, 0.25 μl Go-Taq 2.5 μl PCR buffer (10X), 2 μl 1.5 mM MgCl2 , 0.5 μl of 10 mM dNTP, 0.3 μl of primer.

Article Title: Comparison of different protocols for the extraction of microbial DNA from reef corals
Article Snippet: .. The amplification was performed in a solution containing 1X PCR buffer, 0.2 mM of dNTP, 2.5 mM of MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega), 10 ng of total DNA, 200 μmol of each primer and sterile Milli-Q water for a final volume of 50 μL. .. The reaction was performed in a Mastercycler Gradient (Eppendorf, Hamburg, Germany) under the following conditions: initial denaturation at 94 °C for 3 min, 35 cycles at 94 °C for 1 min, 55 °C for 1 min and 72 °C for 1 min with a final extension at 72 °C for 10 min.

Article Title: Comparison of different protocols for the extraction of microbial DNA from reef corals
Article Snippet: .. The amplification was performed in a solution containing 1X PCR buffer, 0.2 mM of dNTP, 2.0 mM of MgCl2, 0.75 U of recombinant Taq DNA polymerase (Promega), 10 ng of total DNA, 5 pmol of each primer and sterile Milli-Q water for a final volume of 50 μl. .. The reaction was performed in a Mastercycler Gradient (Eppendorf, Hamburg, Germany) under the following conditions: initial denaturation at 94 °C for 30 sec, 35 cycles at 94 °C for 30 sec, 56 °C for 45 sec and 72 °C for 130 sec with a final extension at 72 °C for 7 min.

Article Title: Spatial Arrangement of Polycaprolactone/Collagen Nanofiber Scaffolds Regulates the Wound Healing Related Behaviors of Human Adipose Stromal Cells
Article Snippet: .. The isolated RNA was reverse transcribed into complementary DNA (cDNA) using the SuperScript First-Strand Synthesis System (Promega, Madison, WI), and the cDNA product was then amplified using recombinant Taq DNA polymerase (Promega). .. Expression of integrins α2 , α5 , αV , β1 , β3 , β6 , MMP-1, MMP-2, MMP-9, TGF-β1 , tropoelastin, type I collagen, type III collagen, vimentin, and α-SMA (ACTA-2) were determined over time. β-actin served as housekeeping gene.

Cellular Antioxidant Activity Assay:

Article Title: Comparison of different protocols for the extraction of microbial DNA from reef corals
Article Snippet: PCR-DGGE of 16S and 18S rDNA Amplification of specific regions of the gene encoding the 16S rRNA was performed using the primers U968f (5' GC clamp + AAC GCG AAG AAC CTT AC 3') and L1401r (5' GCG TGT GTA CAA GAC CC 3') ( ). .. The amplification was performed in a solution containing 1X PCR buffer, 0.2 mM of dNTP, 2.5 mM of MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega), 10 ng of total DNA, 200 μmol of each primer and sterile Milli-Q water for a final volume of 50 μL.

Article Title: Comparison of DNA extraction protocols for microbial communities from soil treated with biochar
Article Snippet: PCR-DGGE of the 16S gene and ITS region The amplification of specific regions of the gene encoding the 16S rRNA was performed using the primers U968f GC (5′ CGC CCG CCG CGC GCG GCG GGC GGG GCG GGG GCA CGG GGG GAA CG CGA AGA ACC TTA C 3′) and L1401r (5′ GCG TGT GTA CAA GAC CC 3′) ( ). .. The amplification was performed in a solution containing 1X PCR buffer, 0.2 mM dNTPs, 2.5 mM MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega), 4 ng of total DNA, 200 μmol of each primer and sterile Milli-Q water in a final volume of 50 μL.

Isolation:

Article Title: A time frame permissive for Protein Kinase D2 activity to direct angiogenesis in mouse embryonic stem cells
Article Snippet: Genomic PCR Genomic ESC DNA was isolated using Blood & Tissue Kit (QIAGEN, Germany). .. For PCR reaction, recombinant Taq DNA polymerase (Promega) was used.

Article Title: Spatial Arrangement of Polycaprolactone/Collagen Nanofiber Scaffolds Regulates the Wound Healing Related Behaviors of Human Adipose Stromal Cells
Article Snippet: .. The isolated RNA was reverse transcribed into complementary DNA (cDNA) using the SuperScript First-Strand Synthesis System (Promega, Madison, WI), and the cDNA product was then amplified using recombinant Taq DNA polymerase (Promega). .. Expression of integrins α2 , α5 , αV , β1 , β3 , β6 , MMP-1, MMP-2, MMP-9, TGF-β1 , tropoelastin, type I collagen, type III collagen, vimentin, and α-SMA (ACTA-2) were determined over time. β-actin served as housekeeping gene.

Subcloning:

Article Title: Overexpression of thioredoxin h leads to enhanced activity of starch debranching enzyme (pullulanase) in barley grain
Article Snippet: A 384-bp wtrxh coding region was amplified from pTaM13.38 , which contains a cDNA of wtrxh that served as a template in a PCR to create Xba I and Sac I sites by using the following primers: Wtrxh1 (5′-ata tctaga ATGGCGGCGTCGGCGGCGA -3′) and Wtrxh2R (5′-ata gagctc TTACTGGGCCGCGTGTAG -3′), (see below) (small, italicized letters in the primer denote a restriction enzyme site for subcloning of the DNA fragment containing wtrxh ; underlined letters denote wtrxh sequences). .. PCRs were performed on a thermocycler (MJ Research, Watertown, MA) by using 5 units of recombinant Taq DNA polymerase (Promega) in a 100-μl reaction volume.

Size-exclusion Chromatography:

Article Title: 18S rDNA Sequences from Microeukaryotes Reveal Oil Indicators in Mangrove Sediment
Article Snippet: The amplification was performed in a solution containing 1× PCR buffer, 0.2 mM dNTP, 2.0 mM MgCl2 , 0.75 U of recombinant Taq DNA polymerase (Promega), 10 ng of total DNA, 5 pmol of each primer and sterile Milli-Q water for a final volume of 25 µl. .. The reaction was performed in a thermocycler (Mastercycler Gradient, Eppendorf, Hamburg, Germany) under the following conditions: initial denaturation at 94°C for 30 s, 35 cycles at 94°C for 30 sec, 56°C for 45 sec and 72°C for 130 s with a final extension at 72°C for 7 min.

Article Title: Comparison of different protocols for the extraction of microbial DNA from reef corals
Article Snippet: The amplification was performed in a solution containing 1X PCR buffer, 0.2 mM of dNTP, 2.0 mM of MgCl2, 0.75 U of recombinant Taq DNA polymerase (Promega), 10 ng of total DNA, 5 pmol of each primer and sterile Milli-Q water for a final volume of 50 μl. .. The reaction was performed in a Mastercycler Gradient (Eppendorf, Hamburg, Germany) under the following conditions: initial denaturation at 94 °C for 30 sec, 35 cycles at 94 °C for 30 sec, 56 °C for 45 sec and 72 °C for 130 sec with a final extension at 72 °C for 7 min.

Purification:

Article Title: Overexpression of thioredoxin h leads to enhanced activity of starch debranching enzyme (pullulanase) in barley grain
Article Snippet: PCRs were performed on a thermocycler (MJ Research, Watertown, MA) by using 5 units of recombinant Taq DNA polymerase (Promega) in a 100-μl reaction volume. .. The wtrxh fragment was purified from a 0.7% agarose gel by using a QIAquick gel extraction kit (Qiagen, Chatsworth, CA), digested with Xba I and Sac I and ligated into Xba I/ Sac I-digested pUC19 to generate the pWTRXh-1 plasmid.

Electroporation Bacterial Transformation:

Article Title: Broadcast Spawning Coral Mussismilia hispida Can Vertically Transfer its Associated Bacterial Core
Article Snippet: Symbiodinium Diversity The amplification of a specific region of the 28S rRNA gene of Symbiodinium was performed using the primers D1D2-F and D1D2-R ( ) in a solution containing 1X PCR buffer, 0.2 mM dNTPs, 2.5 mM MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega, Madison, WI, USA), 10–40 ng of total DNA, 0.5 μM of each primer and sterile Milli-Q water in a final volume of 15 μl. .. The reaction was performed in a thermocycler (Mastercycler Gradient, Eppendorf, Hamburg, Germany) with the following conditions: initial denaturing at 94°C for 5 min; 1 cycle at 94°C for 1 min, 56°C for 45 s and 72°C; 29 cycles at 92°C for 1 min, 56°C for 1 min and 72°C for 45 s; and a final extension at 72°C for 10 min. Polymerase chain reaction products were cloned using the pJET 1.2 vector (CloneJET PCR Cloning Kit #K1231, Thermo Scientific) and transformed into Escherichia coli competent cells (Single Step – KRX – Competent Cells, Promega) with the TransformAid Bacterial Transformation Kit (#K2710 Thermo Scientific) according to the manufacturer’s instructions.

Gel Extraction:

Article Title: Overexpression of thioredoxin h leads to enhanced activity of starch debranching enzyme (pullulanase) in barley grain
Article Snippet: PCRs were performed on a thermocycler (MJ Research, Watertown, MA) by using 5 units of recombinant Taq DNA polymerase (Promega) in a 100-μl reaction volume. .. The wtrxh fragment was purified from a 0.7% agarose gel by using a QIAquick gel extraction kit (Qiagen, Chatsworth, CA), digested with Xba I and Sac I and ligated into Xba I/ Sac I-digested pUC19 to generate the pWTRXh-1 plasmid.

Chloramphenicol Acetyltransferase Assay:

Article Title: Comparison of DNA extraction protocols for microbial communities from soil treated with biochar
Article Snippet: The first amplification was performed in a solution containing 1X PCR buffer, 0.2 mM dNTPs, 3.75 mM MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega), 4 ng of total DNA, 200 μmol of each primer, 0.25 μL of bovine serum albumin (BSA) solution (20 mg mL−1 ) and sterile Milli-Q water in a final volume of 25 μL. .. The second amplification was performed with the primers ITS1-F GC (5′ CTT GGT CAT TTA GAG GAA GTA A 3′) ( ) and ITS2 (5′ GCT GCG TTC TTC ATC GAT GC 3′) ( ).

Plasmid Preparation:

Article Title: Overexpression of thioredoxin h leads to enhanced activity of starch debranching enzyme (pullulanase) in barley grain
Article Snippet: Paragraph title: Plasmid 1 (pdBhWTRXhN-1). ... PCRs were performed on a thermocycler (MJ Research, Watertown, MA) by using 5 units of recombinant Taq DNA polymerase (Promega) in a 100-μl reaction volume.

Article Title: Broadcast Spawning Coral Mussismilia hispida Can Vertically Transfer its Associated Bacterial Core
Article Snippet: Symbiodinium Diversity The amplification of a specific region of the 28S rRNA gene of Symbiodinium was performed using the primers D1D2-F and D1D2-R ( ) in a solution containing 1X PCR buffer, 0.2 mM dNTPs, 2.5 mM MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega, Madison, WI, USA), 10–40 ng of total DNA, 0.5 μM of each primer and sterile Milli-Q water in a final volume of 15 μl. .. The reaction was performed in a thermocycler (Mastercycler Gradient, Eppendorf, Hamburg, Germany) with the following conditions: initial denaturing at 94°C for 5 min; 1 cycle at 94°C for 1 min, 56°C for 45 s and 72°C; 29 cycles at 92°C for 1 min, 56°C for 1 min and 72°C for 45 s; and a final extension at 72°C for 10 min. Polymerase chain reaction products were cloned using the pJET 1.2 vector (CloneJET PCR Cloning Kit #K1231, Thermo Scientific) and transformed into Escherichia coli competent cells (Single Step – KRX – Competent Cells, Promega) with the TransformAid Bacterial Transformation Kit (#K2710 Thermo Scientific) according to the manufacturer’s instructions.

SYBR Green Assay:

Article Title: Broadcast Spawning Coral Mussismilia hispida Can Vertically Transfer its Associated Bacterial Core
Article Snippet: Bacterial Profile Specific regions of the gene encoding the 16S rRNA gene were amplified using the primers U968f GC and L1401r ( ) in a solution containing 1X PCR buffer, 0.2 mM dNTPs, 2.5 mM MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega, Madison, WI, USA), 10–40 ng of total DNA, 200 μmol of each primer and sterile Milli-Q water in a final volume of 50 μl. .. Electrophoresis was performed in Tris-acetate-EDTA buffer at 60°C at a constant voltage of 75 V for 16 h. The DGGE gels were stained with SYBR Green (Invitrogen, Carlsbad, CA, USA) and visualized using a Storm 860 Imaging System (GE Healthcare, Milwaukee, WI, USA).

Article Title: Comparison of DNA extraction protocols for microbial communities from soil treated with biochar
Article Snippet: The DGGE gels were stained with SYBR Green (Molecular Probes) and visualised using a Storm 860 Imaging System (GE Healthcare). .. The first amplification was performed in a solution containing 1X PCR buffer, 0.2 mM dNTPs, 3.75 mM MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega), 4 ng of total DNA, 200 μmol of each primer, 0.25 μL of bovine serum albumin (BSA) solution (20 mg mL−1 ) and sterile Milli-Q water in a final volume of 25 μL.

Article Title: Comparison of different protocols for the extraction of microbial DNA from reef corals
Article Snippet: The DGGE gels were stained with Sybr Green (Molecular Probes) and visualized using a Storm 860 Imaging System (GE Healthcare). .. The amplification was performed in a solution containing 1X PCR buffer, 0.2 mM of dNTP, 2.0 mM of MgCl2, 0.75 U of recombinant Taq DNA polymerase (Promega), 10 ng of total DNA, 5 pmol of each primer and sterile Milli-Q water for a final volume of 50 μl.

Selection:

Article Title: Broadcast Spawning Coral Mussismilia hispida Can Vertically Transfer its Associated Bacterial Core
Article Snippet: Symbiodinium Diversity The amplification of a specific region of the 28S rRNA gene of Symbiodinium was performed using the primers D1D2-F and D1D2-R ( ) in a solution containing 1X PCR buffer, 0.2 mM dNTPs, 2.5 mM MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega, Madison, WI, USA), 10–40 ng of total DNA, 0.5 μM of each primer and sterile Milli-Q water in a final volume of 15 μl. .. The RFLPs enabled the selection of distinct Symbiodinium lineages for sequencing.

Agarose Gel Electrophoresis:

Article Title: Overexpression of thioredoxin h leads to enhanced activity of starch debranching enzyme (pullulanase) in barley grain
Article Snippet: PCRs were performed on a thermocycler (MJ Research, Watertown, MA) by using 5 units of recombinant Taq DNA polymerase (Promega) in a 100-μl reaction volume. .. The wtrxh fragment was purified from a 0.7% agarose gel by using a QIAquick gel extraction kit (Qiagen, Chatsworth, CA), digested with Xba I and Sac I and ligated into Xba I/ Sac I-digested pUC19 to generate the pWTRXh-1 plasmid.

Staining:

Article Title: Broadcast Spawning Coral Mussismilia hispida Can Vertically Transfer its Associated Bacterial Core
Article Snippet: Bacterial Profile Specific regions of the gene encoding the 16S rRNA gene were amplified using the primers U968f GC and L1401r ( ) in a solution containing 1X PCR buffer, 0.2 mM dNTPs, 2.5 mM MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega, Madison, WI, USA), 10–40 ng of total DNA, 200 μmol of each primer and sterile Milli-Q water in a final volume of 50 μl. .. Electrophoresis was performed in Tris-acetate-EDTA buffer at 60°C at a constant voltage of 75 V for 16 h. The DGGE gels were stained with SYBR Green (Invitrogen, Carlsbad, CA, USA) and visualized using a Storm 860 Imaging System (GE Healthcare, Milwaukee, WI, USA).

Article Title: Comparison of DNA extraction protocols for microbial communities from soil treated with biochar
Article Snippet: The DGGE gels were stained with SYBR Green (Molecular Probes) and visualised using a Storm 860 Imaging System (GE Healthcare). .. The first amplification was performed in a solution containing 1X PCR buffer, 0.2 mM dNTPs, 3.75 mM MgCl2 , 2.5 U of recombinant Taq DNA polymerase (Promega), 4 ng of total DNA, 200 μmol of each primer, 0.25 μL of bovine serum albumin (BSA) solution (20 mg mL−1 ) and sterile Milli-Q water in a final volume of 25 μL.

Article Title: Comparison of different protocols for the extraction of microbial DNA from reef corals
Article Snippet: The DGGE gels were stained with Sybr Green (Molecular Probes) and visualized using a Storm 860 Imaging System (GE Healthcare). .. The amplification was performed in a solution containing 1X PCR buffer, 0.2 mM of dNTP, 2.0 mM of MgCl2, 0.75 U of recombinant Taq DNA polymerase (Promega), 10 ng of total DNA, 5 pmol of each primer and sterile Milli-Q water for a final volume of 50 μl.

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    Promega taq dna recombinant polymerase
    Taq Dna Recombinant Polymerase, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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