recombinant proteins glutathione sepharose 4 fast flow resin ge healthcare  (GE Healthcare)

 
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    GE Healthcare recombinant proteins glutathione sepharose 4 fast flow resin ge healthcare
    Recombinant Proteins Glutathione Sepharose 4 Fast Flow Resin Ge Healthcare, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant proteins glutathione sepharose 4 fast flow resin ge healthcare/product/GE Healthcare
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    recombinant proteins glutathione sepharose 4 fast flow resin ge healthcare - by Bioz Stars, 2020-10
    99/100 stars

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    Flow Cytometry:

    Article Title: Functional interaction of chloroplast SRP/FtsY with the ALB3 translocase in thylakoids
    Article Snippet: .. Glutathione Sepharose™ fast flow (Amersham Biosciences) was used for initial purification. ..

    Article Title: Characterization of the role of EGF-A of low density lipoprotein receptor in PCSK9 binding
    Article Snippet: .. The GST:EGF-A fusion protein was purified using Glutathione Sepharose 4 Fast Flow (GE Healthcare) affinity gel chromatography according to the manufacturer's protocol. .. The protein was concentrated and further purified using size-exclusion chromatography on a Tricorn Superose 12 10/300 fast-performance liquid chromatography column (GE Healthcare).

    Article Title: Wilms’ tumor gene 1 regulates p63 and promotes cell proliferation in squamous cell carcinoma of the head and neck
    Article Snippet: .. Equivalent amounts of protein lysate were incubated with the anti-WT1 (catalog no. M3561, DAKO, Glostrup, Denmark), anti-IgG (catalog no. 2729S, Millipore, Billerica, U.S.A.) antibodies at 4°C overnight, then incubated with Protein G Sepharose 4 Fast Flow (GE Healthcare, Uppsala, Sweden) at 4°C for 1 hr. .. Immunoprecipitates were washed with lysis buffer three times.

    Chromatography:

    Article Title: Characterization of the role of EGF-A of low density lipoprotein receptor in PCSK9 binding
    Article Snippet: .. The GST:EGF-A fusion protein was purified using Glutathione Sepharose 4 Fast Flow (GE Healthcare) affinity gel chromatography according to the manufacturer's protocol. .. The protein was concentrated and further purified using size-exclusion chromatography on a Tricorn Superose 12 10/300 fast-performance liquid chromatography column (GE Healthcare).

    Purification:

    Article Title: Functional interaction of chloroplast SRP/FtsY with the ALB3 translocase in thylakoids
    Article Snippet: .. Glutathione Sepharose™ fast flow (Amersham Biosciences) was used for initial purification. ..

    Article Title: Characterization of the role of EGF-A of low density lipoprotein receptor in PCSK9 binding
    Article Snippet: .. The GST:EGF-A fusion protein was purified using Glutathione Sepharose 4 Fast Flow (GE Healthcare) affinity gel chromatography according to the manufacturer's protocol. .. The protein was concentrated and further purified using size-exclusion chromatography on a Tricorn Superose 12 10/300 fast-performance liquid chromatography column (GE Healthcare).

    Article Title: Ribosomal Protein S3 Negatively Regulates Unwinding Activity of RecQ-like Helicase 4 through Their Physical Interaction *
    Article Snippet: .. Purification of truncated RPS3 proteins N-RPS3 (1–94 aa) and C-RPS3 (94–244 aa) was done in two steps using nickel and glutathione-Sepharose. .. Induced cell pellets were lysed in 50 m m Tris-HCl, pH 8.0, 200 m m NaCl, 10% glycerol, 0.2% Triton X-100, 2.5 m m 2ME, 1 m m PMSF, and protease inhibitor cocktail).

    Article Title: C-terminal Phosphorylation of LKB1 Is Not Required for Regulation of AMP-activated Protein Kinase, BRSK1, BRSK2, or Cell Cycle Arrest *
    Article Snippet: .. After purification on glutathione-Sepharose, we obtained equal yields of full-length and truncated LKB1L , and both co-purified with FLAG-STRADα and myc -MO25α as expected ( ). ..

    Incubation:

    Article Title: Wilms’ tumor gene 1 regulates p63 and promotes cell proliferation in squamous cell carcinoma of the head and neck
    Article Snippet: .. Equivalent amounts of protein lysate were incubated with the anti-WT1 (catalog no. M3561, DAKO, Glostrup, Denmark), anti-IgG (catalog no. 2729S, Millipore, Billerica, U.S.A.) antibodies at 4°C overnight, then incubated with Protein G Sepharose 4 Fast Flow (GE Healthcare, Uppsala, Sweden) at 4°C for 1 hr. .. Immunoprecipitates were washed with lysis buffer three times.

    Article Title: Regulation of Bone Morphogenetic Protein Signaling by ADP-ribosylation *
    Article Snippet: .. Then proteins were extracted from bacteria using a Triton X-100 containing lysis buffer (50 mm Tris-HCl, pH 7.5, 1 mm EDTA, 100 mm NaCl, 5% glycerol, 0.5% Triton X-100), supplemented with 1 mm DTT and protease inhibitors, and incubated end over end at 4 °C, overnight, with glutathione-Sepharose beads (catalog no. 17-5132-01, lot no. 10172617; GE Healthcare). ..

    other:

    Article Title: Insulin Stimulates Syntaxin4 SNARE Complex Assembly via a Novel Regulatory Mechanism
    Article Snippet: In this case, Sx4-GST bound to glutathione-Sepharose was preincubated with His-Munc18c or was not preincubated prior to the addition of SNAP23 and VAMP2.

    Lysis:

    Article Title: Regulation of Bone Morphogenetic Protein Signaling by ADP-ribosylation *
    Article Snippet: .. Then proteins were extracted from bacteria using a Triton X-100 containing lysis buffer (50 mm Tris-HCl, pH 7.5, 1 mm EDTA, 100 mm NaCl, 5% glycerol, 0.5% Triton X-100), supplemented with 1 mm DTT and protease inhibitors, and incubated end over end at 4 °C, overnight, with glutathione-Sepharose beads (catalog no. 17-5132-01, lot no. 10172617; GE Healthcare). ..

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    GE Healthcare recombinant proteins glutathione sepharose 4 fast flow resin ge healthcare
    Recombinant Proteins Glutathione Sepharose 4 Fast Flow Resin Ge Healthcare, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant proteins glutathione sepharose 4 fast flow resin ge healthcare/product/GE Healthcare
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    recombinant proteins glutathione sepharose 4 fast flow resin ge healthcare - by Bioz Stars, 2020-10
    99/100 stars
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