recombinant mouse il 6r  (R&D Systems)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Name:
    Recombinant Mouse IL 6 IL 6 R alpha Protein Chimera
    Description:
    The Recombinant Mouse IL 6 IL 6 R alpha Protein Chimera from R D Systems is derived from NS0 The Recombinant Mouse IL 6 IL 6 R alpha Protein Chimera has been validated for the following applications Bioactivity
    Catalog Number:
    9038-SR-025
    Price:
    329
    Category:
    Proteins and Enzymes
    Source:
    NS0-derived Recombinant Mouse IL-6/IL-6 R alpha Protein Chimera
    Applications:
    Bioactivity
    Purity:
    >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie« Blue Staining.
    Conjugate:
    Unconjugated
    Size:
    25 ug
    Buy from Supplier


    Structured Review

    R&D Systems recombinant mouse il 6r
    Recombinant Mouse IL 6 IL 6 R alpha Protein Chimera
    The Recombinant Mouse IL 6 IL 6 R alpha Protein Chimera from R D Systems is derived from NS0 The Recombinant Mouse IL 6 IL 6 R alpha Protein Chimera has been validated for the following applications Bioactivity
    https://www.bioz.com/result/recombinant mouse il 6r/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant mouse il 6r - by Bioz Stars, 2021-06
    93/100 stars

    Images

    1) Product Images from "An Autocrine Neuronal Interleukin-6 Loop Mediates Chloride Accumulation and NKCC1 Phosphorylation in Axotomized Sensory Neurons"

    Article Title: An Autocrine Neuronal Interleukin-6 Loop Mediates Chloride Accumulation and NKCC1 Phosphorylation in Axotomized Sensory Neurons

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.3382-11.2011

    Nerve injury upregulates IL-6R expression in adult mice DRG neurons. A , RT-qPCR shows a significant increase in mRNA expression of Il6ra gene in L4–L5 DRGs 3 d after sciatic nerve section ( n , number of experiments with duplicate measure of each gene; ** p
    Figure Legend Snippet: Nerve injury upregulates IL-6R expression in adult mice DRG neurons. A , RT-qPCR shows a significant increase in mRNA expression of Il6ra gene in L4–L5 DRGs 3 d after sciatic nerve section ( n , number of experiments with duplicate measure of each gene; ** p

    Techniques Used: Expressing, Mouse Assay, Quantitative RT-PCR

    IL-6R is expressed in a subset of myelinated sensory neurons. A , Apotome images of transversal slices of axotomized DRG double stained with anti-IL-6R (red) and anti-NF-200 (green) antibodies. The merge image shows colocalization of IL-6R with NF-200-positive sensory neurons. B , Double immunochemistry using anti-TrkB antibody (green) shows IL-6R colocalization with TrkB-positive neurons (merge). C , Double immunochemistry using anti-Parvalbumin antibody (green) shows IL-6R colocalization with parvalbumin-positive neurons (merge). D , Double immunochemistry using anti-TrkA antibody (green) demonstrates no IL6-R colocalization with TrkA-positive neurons (merge). Scale bar, 30 μm.
    Figure Legend Snippet: IL-6R is expressed in a subset of myelinated sensory neurons. A , Apotome images of transversal slices of axotomized DRG double stained with anti-IL-6R (red) and anti-NF-200 (green) antibodies. The merge image shows colocalization of IL-6R with NF-200-positive sensory neurons. B , Double immunochemistry using anti-TrkB antibody (green) shows IL-6R colocalization with TrkB-positive neurons (merge). C , Double immunochemistry using anti-Parvalbumin antibody (green) shows IL-6R colocalization with parvalbumin-positive neurons (merge). D , Double immunochemistry using anti-TrkA antibody (green) demonstrates no IL6-R colocalization with TrkA-positive neurons (merge). Scale bar, 30 μm.

    Techniques Used: Staining

    Il-6 regulates the expression of phospho-NKCC1 close to the plasma membrane of axotomized DRG neurons. A , Transverse section of L4–L6 DRG immunolabeled with an antibody generated against phospho-NKCC1; no signal is observed. B , Five days after nerve injury, phospho-NKCC1 staining is present at/over the plasma membrane in a subset of sensory neurons (arrows). C , Similar to WT DRG, phospho-NKCC1 staining was absent in transverse sections of L4–L6 DRGs from IL6 −/− mice. D , Five days after nerve injury, only a few neurons have a plasma membrane showing phospho-NKCC1 staining in DRG from IL-6 −/− mice (arrow). E , Confocal microscopy of double-immunofluorescent labeling for phospho-NKCC1 (p-NKCC1) and glutamine synthase (GS) shows no coexpression. F , Confocal microscopy of double-immunofluorescent labeling for p-NKCC1 and IL-6R shows expression in the same cell type. Merge image shows a co expression that appears as an alternate punctiform staining at the plasma level. Scale bars, 30 μm. Insets in merge images are 3× magnifications of the region marked by white asterisk. A–D , 20× objective; E , F , 40× objective.
    Figure Legend Snippet: Il-6 regulates the expression of phospho-NKCC1 close to the plasma membrane of axotomized DRG neurons. A , Transverse section of L4–L6 DRG immunolabeled with an antibody generated against phospho-NKCC1; no signal is observed. B , Five days after nerve injury, phospho-NKCC1 staining is present at/over the plasma membrane in a subset of sensory neurons (arrows). C , Similar to WT DRG, phospho-NKCC1 staining was absent in transverse sections of L4–L6 DRGs from IL6 −/− mice. D , Five days after nerve injury, only a few neurons have a plasma membrane showing phospho-NKCC1 staining in DRG from IL-6 −/− mice (arrow). E , Confocal microscopy of double-immunofluorescent labeling for phospho-NKCC1 (p-NKCC1) and glutamine synthase (GS) shows no coexpression. F , Confocal microscopy of double-immunofluorescent labeling for p-NKCC1 and IL-6R shows expression in the same cell type. Merge image shows a co expression that appears as an alternate punctiform staining at the plasma level. Scale bars, 30 μm. Insets in merge images are 3× magnifications of the region marked by white asterisk. A–D , 20× objective; E , F , 40× objective.

    Techniques Used: Expressing, Immunolabeling, Generated, Staining, Mouse Assay, Confocal Microscopy, Labeling

    Related Articles

    other:

    Article Title: In vitro IL-6/IL-6R Trans-Signaling in Fibroblasts Releases Cytokines That May Be Linked to the Pathogenesis of IgG4-Related Disease
    Article Snippet: In this study, different concentrations of IL-6/IL-6R (0, 10, 20, 50, and 100 ng/ml) were used to stimulate AAFs and the levels of BAFF, IL-7, IL-12 p70, and IL-23 of supernatant were detected at different time points (24 and 48 h) to observe the effects of IL-6/IL-6R on AAFs.

    Article Title: Ovarian function’s role during cancer cachexia progression in the female mouse
    Article Snippet: The lower limits of detection for each assay were 15, 500, and 62 pg/ml for standard mouse IL-6, sIL-6r, and leptin, respectively.

    Article Title: In vitro IL-6/IL-6R Trans-Signaling in Fibroblasts Releases Cytokines That May Be Linked to the Pathogenesis of IgG4-Related Disease
    Article Snippet: The JAK1 inhibitor (Itacitinib, 10 ng/ml), JAK2 inhibitor (AG490), STAT3 inhibitor (S31-201), and Akt inhibitor (LY294002) were used to pretreat AAFs to explore the mechanism by which IL-6/IL-6R induces the production of BAFF, IL-7, IL-12 p70, and IL-23 in AAFs.

    Recombinant:

    Article Title: Expression and Function of TNF and IL-1 Receptors on Human Regulatory T Cells
    Article Snippet: Recombinant IL-1β and sIL-1R2 were purchased from R & D systems. .. Recombinant IL-6 and IL-8 used to generate standard curves were provided with the BD CBA flex set kit. .. Statistical analysisReceptor expression statistics in total PBMC, CD4+ cells, and FOXP3 overexpressing cells were done using a paired, two tailed t-test.

    Crocin Bleaching Assay:

    Article Title: Expression and Function of TNF and IL-1 Receptors on Human Regulatory T Cells
    Article Snippet: Recombinant IL-1β and sIL-1R2 were purchased from R & D systems. .. Recombinant IL-6 and IL-8 used to generate standard curves were provided with the BD CBA flex set kit. .. Statistical analysisReceptor expression statistics in total PBMC, CD4+ cells, and FOXP3 overexpressing cells were done using a paired, two tailed t-test.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Fabrication of Bioactive Inverted Colloidal Crystal Scaffolds Using Expanded Polystyrene Beads
    Article Snippet: BMSCs were isolated from the bone by centrifugation and cultured in alpha-Modified Eagle's Medium supplemented with 10% fetal bovine serum (F0926; Sigma) and 1% penicillin-streptomycin (15140122; ThermoFisher). .. Enzyme-linked immunosorbent assay of mouse IL-6, osteoprotegerin, and receptor activator of nuclear factor kappa-B ligandEPS scaffolds with and without collagen were seeded with 500,000 adherent eGFP BMSCs and cultured in the wells of a 48-well plate. ..

    Article Title: The role of myeloid-derived suppressor cells in endometrial cancer displaying systemic inflammatory response: clinical and preclinical investigations
    Article Snippet: Enzyme-linked immunosorbent assay (ELISA)The concentrations of human G-CSF and mouse G-CSF were measured using a Human G-CSF Quantikine ELISA Kit (Cat No. DCS50) and a Mouse G-CSF Quantikine ELISA Kit (Cat No. MCS00) obtained from R & D systems (Minneapolis, MN, USA), respectively. .. The concentrations of human IL-6 and mouse IL-6 were measured using a Human IL-6 ELISA Ready-SET-Go! .. Kit (Cat No. 501128847) and a Mouse IL-6 ELISA Ready-SET-Go!

    Article Title: LipA, a Tyrosine and Lipid Phosphatase Involved in the Virulence of Listeria monocytogenes ▿ ▿ †
    Article Snippet: Blood was collected from the retro-orbital sinus of mice. .. Serum cytokine levels were measured by enzyme-linked immunosorbent assay (ELISA) using mouse tumor necrosis factor alpha (TNF-α) DuoSet, mouse interleukin-6 (IL-6) DuoSet, or mouse gamma interferon (IFN-γ) DuoSet by following the manufacturer's recommendations (R & D Systems). ..

    Cell Culture:

    Article Title: Fabrication of Bioactive Inverted Colloidal Crystal Scaffolds Using Expanded Polystyrene Beads
    Article Snippet: BMSCs were isolated from the bone by centrifugation and cultured in alpha-Modified Eagle's Medium supplemented with 10% fetal bovine serum (F0926; Sigma) and 1% penicillin-streptomycin (15140122; ThermoFisher). .. Enzyme-linked immunosorbent assay of mouse IL-6, osteoprotegerin, and receptor activator of nuclear factor kappa-B ligandEPS scaffolds with and without collagen were seeded with 500,000 adherent eGFP BMSCs and cultured in the wells of a 48-well plate. ..

    Concentration Assay:

    Article Title: Dominant-negative mutations in human IL6ST underlie hyper-IgE syndrome
    Article Snippet: IFN-α (Intron A) was purchased from Merck. .. IL-6, IL-6–IL-6Rα, IL-11, IL-27, LIF, and OSM were all used at a final concentration of 100 ng/ml. .. IFN-α was used at a final concentration of 105 U/ml.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    R&D Systems recombinant human il 6 receptor
    Induction of liver acute phase response gene expression in STAT3SA/− mice. Littermate STAT3+/+ and STAT3SA/− mice were injected with turpentine subcutaneously or recombinant <t>IL-6</t> intraperitoneally. After 12 h of turpentine injection or 6 h of IL-6 injection, liver total RNA was prepared from each animal and analyzed by RT-PCR. As negative controls, mice were either left untreated or injected with sterile pyrogen-free saline. SAA, serum amyloids A; SAP, serum amyloids P; FB, fibrinogen; HP, haptoglobin; HPX, hemopexin; AGP, α1-acid glycoprotein.
    Recombinant Human Il 6 Receptor, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant human il 6 receptor/product/R&D Systems
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recombinant human il 6 receptor - by Bioz Stars, 2021-06
    94/100 stars
      Buy from Supplier

    97
    R&D Systems rabbit monoclonal antibody against il 6r
    <t>IL-6R-mediated</t> IL-6 inhibition of TRPM7 inward currents. (A) Representative raw traces of TRPM7 current in cortical neurons perfused with IL-6R antibodies in Ca 2+ -free extracellular solution. (B) The inhibition of the TRPM7-like inward current by IL-6 was weakened in the presence of IL-6 receptor antibodies. (C) Representative raw traces of TRPM7 current in HEK293-TRPM7/WT cells perfused with IL-6 or sIL-6R only, or IL-6/sIL-6R, in Ca 2+ -free extracellular solution. (D) Co-treatment with IL-6 and sIL-6R produced maximal inhibition of inward TRPM7 currents in HEK293-TRPM7 cells.
    Rabbit Monoclonal Antibody Against Il 6r, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit monoclonal antibody against il 6r/product/R&D Systems
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal antibody against il 6r - by Bioz Stars, 2021-06
    97/100 stars
      Buy from Supplier

    95
    R&D Systems il 6 il 6r
    The secretion of cytokines (IL-7, BAFF, IL-12 p70, and IL-23) in the supernatant following stimulation with <t>IL-6/IL-6R</t> (50 ng/ml) with or without different inhibitors; ** p
    Il 6 Il 6r, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 6 il 6r/product/R&D Systems
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    il 6 il 6r - by Bioz Stars, 2021-06
    95/100 stars
      Buy from Supplier

    93
    R&D Systems il 6r alpha antibody
    <t>IL-6R</t> alpha expression in human neutrophils and association of the rs2228145 genotype with secretion of sIL-6R by leukocytes. ( A ) Serum sIL-6R concentration from the rs2228145 genotype groups was measured by ELISA. ( B ) Immunofluorescence of IL-6R alpha AF647and IgG1 AF647 isotype matched control in neutrophils. Merged images show DAPI and IL-6R AF647 or IgG1 AF647. Magnification x63 with x5 zoom. ( C ) Expression of IL-6R alpha in neutrophils (NEU) and monocytes (MONO) analyzed by flow cytometry. Red (unstained), blue (IgG1) and green (IL-6R AF647). Monocytes were gated from the peripheral blood mononuclear cell population based on their forward scatter and side scatter properties. ( D ) Immunocytochemistry of IL-6R alpha and IgG1 isotype matched control in neutrophils. Magnification ×100. Data are representative of three independent experiments. ( E ) sIL-6R release from the rs2228145 genotyped polymorphonuclear neutrophils (PMN) stimulated for 30 min with fMLP or PMA ( F,G ) sIL-6R release from the rs2228145 genotyped peripheral blood mononuclear cells (PBMCs) stimulated for 30 min with PMA. sIL-6R concentrations refer to the difference between stimulated and control levels for B–D. Data represent the median with interquartile range. * P
    Il 6r Alpha Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 6r alpha antibody/product/R&D Systems
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    il 6r alpha antibody - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

    Image Search Results


    Induction of liver acute phase response gene expression in STAT3SA/− mice. Littermate STAT3+/+ and STAT3SA/− mice were injected with turpentine subcutaneously or recombinant IL-6 intraperitoneally. After 12 h of turpentine injection or 6 h of IL-6 injection, liver total RNA was prepared from each animal and analyzed by RT-PCR. As negative controls, mice were either left untreated or injected with sterile pyrogen-free saline. SAA, serum amyloids A; SAP, serum amyloids P; FB, fibrinogen; HP, haptoglobin; HPX, hemopexin; AGP, α1-acid glycoprotein.

    Journal: Molecular and Cellular Biology

    Article Title: Essential Role of STAT3 in Postnatal Survival and Growth Revealed by Mice Lacking STAT3 Serine 727 Phosphorylation

    doi: 10.1128/MCB.24.1.407-419.2004

    Figure Lengend Snippet: Induction of liver acute phase response gene expression in STAT3SA/− mice. Littermate STAT3+/+ and STAT3SA/− mice were injected with turpentine subcutaneously or recombinant IL-6 intraperitoneally. After 12 h of turpentine injection or 6 h of IL-6 injection, liver total RNA was prepared from each animal and analyzed by RT-PCR. As negative controls, mice were either left untreated or injected with sterile pyrogen-free saline. SAA, serum amyloids A; SAP, serum amyloids P; FB, fibrinogen; HP, haptoglobin; HPX, hemopexin; AGP, α1-acid glycoprotein.

    Article Snippet: Twenty-four hours after transfection, cells were treated with human IL-6 (12.5 ng/ml; SERVA) and recombinant human IL-6 receptor (IL-6R; 50 ng/ml; R & D Systems) or OSM (25 ng/ml; R & D Systems) for 6 h before cells were harvested for the luciferase assay.

    Techniques: Expressing, Mouse Assay, Injection, Recombinant, Reverse Transcription Polymerase Chain Reaction

    IL-6R-mediated IL-6 inhibition of TRPM7 inward currents. (A) Representative raw traces of TRPM7 current in cortical neurons perfused with IL-6R antibodies in Ca 2+ -free extracellular solution. (B) The inhibition of the TRPM7-like inward current by IL-6 was weakened in the presence of IL-6 receptor antibodies. (C) Representative raw traces of TRPM7 current in HEK293-TRPM7/WT cells perfused with IL-6 or sIL-6R only, or IL-6/sIL-6R, in Ca 2+ -free extracellular solution. (D) Co-treatment with IL-6 and sIL-6R produced maximal inhibition of inward TRPM7 currents in HEK293-TRPM7 cells.

    Journal: PLoS ONE

    Article Title: Regulation of TRPM7 Function by IL-6 through the JAK2-STAT3 Signaling Pathway

    doi: 10.1371/journal.pone.0152120

    Figure Lengend Snippet: IL-6R-mediated IL-6 inhibition of TRPM7 inward currents. (A) Representative raw traces of TRPM7 current in cortical neurons perfused with IL-6R antibodies in Ca 2+ -free extracellular solution. (B) The inhibition of the TRPM7-like inward current by IL-6 was weakened in the presence of IL-6 receptor antibodies. (C) Representative raw traces of TRPM7 current in HEK293-TRPM7/WT cells perfused with IL-6 or sIL-6R only, or IL-6/sIL-6R, in Ca 2+ -free extracellular solution. (D) Co-treatment with IL-6 and sIL-6R produced maximal inhibition of inward TRPM7 currents in HEK293-TRPM7 cells.

    Article Snippet: Compounds Pharmacological compounds used in this study included recombinant human IL-6 (Preprotech, London, UK), recombinant human IL-6R (Preprotech), rabbit monoclonal antibody against IL-6R (R & D Systems, Inc. Minneapolis, MN, USA), and goat polyclonal antibody against TRPM7 (Santa Cruz Biotechnology, Santa Cruz, CA, USA).

    Techniques: Inhibition, Produced

    The inhibitory effect of IL-6/IL-6R on TRPM7 currents is blocked by the STAT3 inhibitor HO-3867, but not by the inhibitor MAPK signaling pathway. (A) Relative currents normalized to TRPM7 currents recorded during perfusion with divalent-free extracellular solution. The specific STAT3 inhibitor HO-3867 did not inhibit TRPM7 inward currents at -100 mV, but blocked the effect of IL-6/sIL-6R (n = 3, * p

    Journal: PLoS ONE

    Article Title: Regulation of TRPM7 Function by IL-6 through the JAK2-STAT3 Signaling Pathway

    doi: 10.1371/journal.pone.0152120

    Figure Lengend Snippet: The inhibitory effect of IL-6/IL-6R on TRPM7 currents is blocked by the STAT3 inhibitor HO-3867, but not by the inhibitor MAPK signaling pathway. (A) Relative currents normalized to TRPM7 currents recorded during perfusion with divalent-free extracellular solution. The specific STAT3 inhibitor HO-3867 did not inhibit TRPM7 inward currents at -100 mV, but blocked the effect of IL-6/sIL-6R (n = 3, * p

    Article Snippet: Compounds Pharmacological compounds used in this study included recombinant human IL-6 (Preprotech, London, UK), recombinant human IL-6R (Preprotech), rabbit monoclonal antibody against IL-6R (R & D Systems, Inc. Minneapolis, MN, USA), and goat polyclonal antibody against TRPM7 (Santa Cruz Biotechnology, Santa Cruz, CA, USA).

    Techniques:

    The inhibitory effect of IL-6/IL-6R on TRPM7 currents is blocked by the JAK2 inhibitor AG490, but not by inhibitors of PI3K and PLC. (A) Currents recorded when cells were perfused with divalent ion-free extracellular solution, IL-6/sIL-6R, AG490, or AG490 combined with IL-6/sIL-6R, respectively. (B) The inhibitory role of IL-6 on TRPM7 inward currents could be blocked by the JAK2 inhibitor AG490 (n = 11) (C) Current traces recorded with perfusion of divalent ion-free extracellular solution, IL-6/sIL-6R, wortmannin, or wortmannin combined with IL-6/sIL-6R, respectively. (D) Relative currents normalized to the TRPM7 current at -100 mV and recorded during perfusion of divalent ion-free extracellular solution. The specific PI3K inhibitor wortmannin did not block the effect of IL-6 on TRPM7 inward currents at -100 mV (n = 6). (E) Current traces recorded during perfusion of divalent ion-free extracellular solution, IL-6/sIL-6R, U73122, or U73122 combined with IL-6/sIL-6R, respectively. (F) Relative currents normalized to TRPM7 currents recorded with perfusion of divalent ion-free extracellular solution. The PLC inhibitor U73122 inhibited TRPM7 inward currents at -100 mV, but did not block the effects of IL-6/sIL-6R (n = 7, * p

    Journal: PLoS ONE

    Article Title: Regulation of TRPM7 Function by IL-6 through the JAK2-STAT3 Signaling Pathway

    doi: 10.1371/journal.pone.0152120

    Figure Lengend Snippet: The inhibitory effect of IL-6/IL-6R on TRPM7 currents is blocked by the JAK2 inhibitor AG490, but not by inhibitors of PI3K and PLC. (A) Currents recorded when cells were perfused with divalent ion-free extracellular solution, IL-6/sIL-6R, AG490, or AG490 combined with IL-6/sIL-6R, respectively. (B) The inhibitory role of IL-6 on TRPM7 inward currents could be blocked by the JAK2 inhibitor AG490 (n = 11) (C) Current traces recorded with perfusion of divalent ion-free extracellular solution, IL-6/sIL-6R, wortmannin, or wortmannin combined with IL-6/sIL-6R, respectively. (D) Relative currents normalized to the TRPM7 current at -100 mV and recorded during perfusion of divalent ion-free extracellular solution. The specific PI3K inhibitor wortmannin did not block the effect of IL-6 on TRPM7 inward currents at -100 mV (n = 6). (E) Current traces recorded during perfusion of divalent ion-free extracellular solution, IL-6/sIL-6R, U73122, or U73122 combined with IL-6/sIL-6R, respectively. (F) Relative currents normalized to TRPM7 currents recorded with perfusion of divalent ion-free extracellular solution. The PLC inhibitor U73122 inhibited TRPM7 inward currents at -100 mV, but did not block the effects of IL-6/sIL-6R (n = 7, * p

    Article Snippet: Compounds Pharmacological compounds used in this study included recombinant human IL-6 (Preprotech, London, UK), recombinant human IL-6R (Preprotech), rabbit monoclonal antibody against IL-6R (R & D Systems, Inc. Minneapolis, MN, USA), and goat polyclonal antibody against TRPM7 (Santa Cruz Biotechnology, Santa Cruz, CA, USA).

    Techniques: Planar Chromatography, Blocking Assay

    The secretion of cytokines (IL-7, BAFF, IL-12 p70, and IL-23) in the supernatant following stimulation with IL-6/IL-6R (50 ng/ml) with or without different inhibitors; ** p

    Journal: Frontiers in Immunology

    Article Title: In vitro IL-6/IL-6R Trans-Signaling in Fibroblasts Releases Cytokines That May Be Linked to the Pathogenesis of IgG4-Related Disease

    doi: 10.3389/fimmu.2020.01272

    Figure Lengend Snippet: The secretion of cytokines (IL-7, BAFF, IL-12 p70, and IL-23) in the supernatant following stimulation with IL-6/IL-6R (50 ng/ml) with or without different inhibitors; ** p

    Article Snippet: The JAK1 inhibitor (Itacitinib, 10 ng/ml), JAK2 inhibitor (AG490), STAT3 inhibitor (S31-201), and Akt inhibitor (LY294002) were used to pretreat AAFs to explore the mechanism by which IL-6/IL-6R induces the production of BAFF, IL-7, IL-12 p70, and IL-23 in AAFs.

    Techniques:

    The level of IL-6 and IL-6R in the serum and tissues of IgG4-RD and the correlation between serum IL-6 and disease activity. (A) The level of serum IL-6 ( N = 74) and IL-6R ( N = 76) was significantly higher in the active IgG4-RD patients than those in the inactive patients, as well as that of the healthy controls ( p

    Journal: Frontiers in Immunology

    Article Title: In vitro IL-6/IL-6R Trans-Signaling in Fibroblasts Releases Cytokines That May Be Linked to the Pathogenesis of IgG4-Related Disease

    doi: 10.3389/fimmu.2020.01272

    Figure Lengend Snippet: The level of IL-6 and IL-6R in the serum and tissues of IgG4-RD and the correlation between serum IL-6 and disease activity. (A) The level of serum IL-6 ( N = 74) and IL-6R ( N = 76) was significantly higher in the active IgG4-RD patients than those in the inactive patients, as well as that of the healthy controls ( p

    Article Snippet: The JAK1 inhibitor (Itacitinib, 10 ng/ml), JAK2 inhibitor (AG490), STAT3 inhibitor (S31-201), and Akt inhibitor (LY294002) were used to pretreat AAFs to explore the mechanism by which IL-6/IL-6R induces the production of BAFF, IL-7, IL-12 p70, and IL-23 in AAFs.

    Techniques: Activity Assay

    Exogenous IL-6/IL-6R promotes the production of Tfh cell and B cell differentiation factors. The secretion of cytokines (IL-7, BAFF, IL-12 p70, and IL-23) in the supernatant after stimulation of IL-6/IL-6R at 24 h and 48 h ( n = 6). * p

    Journal: Frontiers in Immunology

    Article Title: In vitro IL-6/IL-6R Trans-Signaling in Fibroblasts Releases Cytokines That May Be Linked to the Pathogenesis of IgG4-Related Disease

    doi: 10.3389/fimmu.2020.01272

    Figure Lengend Snippet: Exogenous IL-6/IL-6R promotes the production of Tfh cell and B cell differentiation factors. The secretion of cytokines (IL-7, BAFF, IL-12 p70, and IL-23) in the supernatant after stimulation of IL-6/IL-6R at 24 h and 48 h ( n = 6). * p

    Article Snippet: The JAK1 inhibitor (Itacitinib, 10 ng/ml), JAK2 inhibitor (AG490), STAT3 inhibitor (S31-201), and Akt inhibitor (LY294002) were used to pretreat AAFs to explore the mechanism by which IL-6/IL-6R induces the production of BAFF, IL-7, IL-12 p70, and IL-23 in AAFs.

    Techniques: Cell Differentiation

    IL-6R alpha expression in human neutrophils and association of the rs2228145 genotype with secretion of sIL-6R by leukocytes. ( A ) Serum sIL-6R concentration from the rs2228145 genotype groups was measured by ELISA. ( B ) Immunofluorescence of IL-6R alpha AF647and IgG1 AF647 isotype matched control in neutrophils. Merged images show DAPI and IL-6R AF647 or IgG1 AF647. Magnification x63 with x5 zoom. ( C ) Expression of IL-6R alpha in neutrophils (NEU) and monocytes (MONO) analyzed by flow cytometry. Red (unstained), blue (IgG1) and green (IL-6R AF647). Monocytes were gated from the peripheral blood mononuclear cell population based on their forward scatter and side scatter properties. ( D ) Immunocytochemistry of IL-6R alpha and IgG1 isotype matched control in neutrophils. Magnification ×100. Data are representative of three independent experiments. ( E ) sIL-6R release from the rs2228145 genotyped polymorphonuclear neutrophils (PMN) stimulated for 30 min with fMLP or PMA ( F,G ) sIL-6R release from the rs2228145 genotyped peripheral blood mononuclear cells (PBMCs) stimulated for 30 min with PMA. sIL-6R concentrations refer to the difference between stimulated and control levels for B–D. Data represent the median with interquartile range. * P

    Journal: Human Molecular Genetics

    Article Title: Neutrophil-mediated IL-6 receptor trans-signaling and the risk of chronic obstructive pulmonary disease and asthma

    doi: 10.1093/hmg/ddx053

    Figure Lengend Snippet: IL-6R alpha expression in human neutrophils and association of the rs2228145 genotype with secretion of sIL-6R by leukocytes. ( A ) Serum sIL-6R concentration from the rs2228145 genotype groups was measured by ELISA. ( B ) Immunofluorescence of IL-6R alpha AF647and IgG1 AF647 isotype matched control in neutrophils. Merged images show DAPI and IL-6R AF647 or IgG1 AF647. Magnification x63 with x5 zoom. ( C ) Expression of IL-6R alpha in neutrophils (NEU) and monocytes (MONO) analyzed by flow cytometry. Red (unstained), blue (IgG1) and green (IL-6R AF647). Monocytes were gated from the peripheral blood mononuclear cell population based on their forward scatter and side scatter properties. ( D ) Immunocytochemistry of IL-6R alpha and IgG1 isotype matched control in neutrophils. Magnification ×100. Data are representative of three independent experiments. ( E ) sIL-6R release from the rs2228145 genotyped polymorphonuclear neutrophils (PMN) stimulated for 30 min with fMLP or PMA ( F,G ) sIL-6R release from the rs2228145 genotyped peripheral blood mononuclear cells (PBMCs) stimulated for 30 min with PMA. sIL-6R concentrations refer to the difference between stimulated and control levels for B–D. Data represent the median with interquartile range. * P

    Article Snippet: Slides were washed in PBS-Tween, blocked with Hydrogen Peroxidase solution (Dako, UK) and stained with IL-6R alpha antibody (R & D Systems) or mouse IgG1 isotype (R & D Systems) control for 1 h at room temperature.

    Techniques: Expressing, Concentration Assay, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Flow Cytometry, Cytometry, Immunocytochemistry