gene exp tubb3 hs00801390 s1  (Thermo Fisher)


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    Thermo Fisher gene exp tubb3 hs00801390 s1
    Gene Exp Tubb3 Hs00801390 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp tubb3 hs00801390 s1/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gene exp tubb3 hs00801390 s1 - by Bioz Stars, 2023-02
    99/100 stars

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    tubb3  (Thermo Fisher)


    Bioz Verified Symbol Thermo Fisher is a verified supplier
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    Thermo Fisher tubb3
    NSC to PN differentiation in dECM tunicate scaffolds. (A-C) The NSC-loaded dECM tunicate scaffolds under the light microscope: (A) dECM tunicate scaffold without cells, (B) scaffold just after cell loading, and the cells appear rounded and floated over the scaffold, and (C) cells attached to the scaffolds on day 3 of seeding. The scaffolds appeared impermeable to light by day 3 of seeding (scale bars = 125 µm). (D-F) SEM images of the NSC-loaded tunicate dECM scaffolds: (D) Day 3 of NSC culture on the scaffolds showing fibroblast-like morphology, (E) the NSCs changed to more rounded cell appearance by day 7, and (F) formation of extended peripheral neuron fibers by day 12 of PN induction (scale bars = 50 µm). (G) The cell-free control scaffold with DAPI and NEFH staining. (H) DAPI and NEFH staining on day 3 of PN induction. (I) Expression of NEFH protein on day 12 in the induced NSCs-loaded scaffolds indicate the differentiation of the NSCs to PN (scale bars = 100 µm). (J-L) Live-dead staining on cells grown on the tunicate dECM scaffolds showed an increase in number of green florescent cells over time and proliferation of thread-like structures by day 12 (scale bars = 100 µm). (M) Gene expression studies using qPCR experiments; the relative mRNA expression of NEFH and PRPH were increased to four- to five-fold on day 12 of the PN induction, compared to the day 3 samples, while the stemness marker HNK1 expression was remarkably reduced, indicating the differentiation of NSCs to PN. Day 12 neurons also showed increased levels of the pan neuronal marker <t>TUBB3</t> . The gene expression was normalized to house-keeping gene GAPDH . Data reported as mean ± SD ( n = 3; * P < 0.05, ** P < 0.01, *** P < 0.001, by two-tailed Student’s t -test between test and control samples). (N) AlamarBlue cell proliferation assay showed significant cell proliferation on day 12 compared to day 3, P < 0.05.
    Tubb3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tubb3 - by Bioz Stars, 2023-02
    86/100 stars

    Images

    1) Product Images from "Bioprinting of Human Neural Tissues Using a Sustainable Marine Tunicate-Derived Bioink for Translational Medicine Applications"

    Article Title: Bioprinting of Human Neural Tissues Using a Sustainable Marine Tunicate-Derived Bioink for Translational Medicine Applications

    Journal: International Journal of Bioprinting

    doi: 10.18063/ijb.v8i4.604

    NSC to PN differentiation in dECM tunicate scaffolds. (A-C) The NSC-loaded dECM tunicate scaffolds under the light microscope: (A) dECM tunicate scaffold without cells, (B) scaffold just after cell loading, and the cells appear rounded and floated over the scaffold, and (C) cells attached to the scaffolds on day 3 of seeding. The scaffolds appeared impermeable to light by day 3 of seeding (scale bars = 125 µm). (D-F) SEM images of the NSC-loaded tunicate dECM scaffolds: (D) Day 3 of NSC culture on the scaffolds showing fibroblast-like morphology, (E) the NSCs changed to more rounded cell appearance by day 7, and (F) formation of extended peripheral neuron fibers by day 12 of PN induction (scale bars = 50 µm). (G) The cell-free control scaffold with DAPI and NEFH staining. (H) DAPI and NEFH staining on day 3 of PN induction. (I) Expression of NEFH protein on day 12 in the induced NSCs-loaded scaffolds indicate the differentiation of the NSCs to PN (scale bars = 100 µm). (J-L) Live-dead staining on cells grown on the tunicate dECM scaffolds showed an increase in number of green florescent cells over time and proliferation of thread-like structures by day 12 (scale bars = 100 µm). (M) Gene expression studies using qPCR experiments; the relative mRNA expression of NEFH and PRPH were increased to four- to five-fold on day 12 of the PN induction, compared to the day 3 samples, while the stemness marker HNK1 expression was remarkably reduced, indicating the differentiation of NSCs to PN. Day 12 neurons also showed increased levels of the pan neuronal marker TUBB3 . The gene expression was normalized to house-keeping gene GAPDH . Data reported as mean ± SD ( n = 3; * P < 0.05, ** P < 0.01, *** P < 0.001, by two-tailed Student’s t -test between test and control samples). (N) AlamarBlue cell proliferation assay showed significant cell proliferation on day 12 compared to day 3, P < 0.05.
    Figure Legend Snippet: NSC to PN differentiation in dECM tunicate scaffolds. (A-C) The NSC-loaded dECM tunicate scaffolds under the light microscope: (A) dECM tunicate scaffold without cells, (B) scaffold just after cell loading, and the cells appear rounded and floated over the scaffold, and (C) cells attached to the scaffolds on day 3 of seeding. The scaffolds appeared impermeable to light by day 3 of seeding (scale bars = 125 µm). (D-F) SEM images of the NSC-loaded tunicate dECM scaffolds: (D) Day 3 of NSC culture on the scaffolds showing fibroblast-like morphology, (E) the NSCs changed to more rounded cell appearance by day 7, and (F) formation of extended peripheral neuron fibers by day 12 of PN induction (scale bars = 50 µm). (G) The cell-free control scaffold with DAPI and NEFH staining. (H) DAPI and NEFH staining on day 3 of PN induction. (I) Expression of NEFH protein on day 12 in the induced NSCs-loaded scaffolds indicate the differentiation of the NSCs to PN (scale bars = 100 µm). (J-L) Live-dead staining on cells grown on the tunicate dECM scaffolds showed an increase in number of green florescent cells over time and proliferation of thread-like structures by day 12 (scale bars = 100 µm). (M) Gene expression studies using qPCR experiments; the relative mRNA expression of NEFH and PRPH were increased to four- to five-fold on day 12 of the PN induction, compared to the day 3 samples, while the stemness marker HNK1 expression was remarkably reduced, indicating the differentiation of NSCs to PN. Day 12 neurons also showed increased levels of the pan neuronal marker TUBB3 . The gene expression was normalized to house-keeping gene GAPDH . Data reported as mean ± SD ( n = 3; * P < 0.05, ** P < 0.01, *** P < 0.001, by two-tailed Student’s t -test between test and control samples). (N) AlamarBlue cell proliferation assay showed significant cell proliferation on day 12 compared to day 3, P < 0.05.

    Techniques Used: Light Microscopy, Staining, Expressing, Marker, Two Tailed Test, Proliferation Assay

    NSC to PN differentiation in bioprinted neural tissue constructs. (A) DAPI staining on day 1 post-printing. (B) DAPI staining and NEFH immunofluorescence staining on day 3 of the neural induction showed no NEFH expression. (C) DAPI staining and NEFH immunofluorescence staining on day 12 of the neural induction showed high NEFH expression, indicating PN differentiation. Scale bars = 100 µm. (D-F) SEM images of the bioprinted tissue constructs on day 3. (G-I) SEM images of the bioprinted tissue constructs on day 7. (J-L) SEM images of the differentiated neural tissue construct on day 12 showed remarkable neural cell morphology and neural filament formation (yellow arrows), compared to day 3 and day 7 post-induction. The direction of neural filament formed were perpendicular to the printing direction (blue arrows), which requires further investigation. (M) mRNA expression of PN markers PRPH and NEFH were upregulated on day 12 of PN induction compared to day 3. The stemness marker HNK1 was significantly downregulated and the change in the pan neural marker TUBB3 was non-significant (NS). Data are expressed as mean+SD ( n = 3; * P < 0.05, *** P < 0.001, by two- tailed Student’s t -test between test and control samples).
    Figure Legend Snippet: NSC to PN differentiation in bioprinted neural tissue constructs. (A) DAPI staining on day 1 post-printing. (B) DAPI staining and NEFH immunofluorescence staining on day 3 of the neural induction showed no NEFH expression. (C) DAPI staining and NEFH immunofluorescence staining on day 12 of the neural induction showed high NEFH expression, indicating PN differentiation. Scale bars = 100 µm. (D-F) SEM images of the bioprinted tissue constructs on day 3. (G-I) SEM images of the bioprinted tissue constructs on day 7. (J-L) SEM images of the differentiated neural tissue construct on day 12 showed remarkable neural cell morphology and neural filament formation (yellow arrows), compared to day 3 and day 7 post-induction. The direction of neural filament formed were perpendicular to the printing direction (blue arrows), which requires further investigation. (M) mRNA expression of PN markers PRPH and NEFH were upregulated on day 12 of PN induction compared to day 3. The stemness marker HNK1 was significantly downregulated and the change in the pan neural marker TUBB3 was non-significant (NS). Data are expressed as mean+SD ( n = 3; * P < 0.05, *** P < 0.001, by two- tailed Student’s t -test between test and control samples).

    Techniques Used: Construct, Staining, Immunofluorescence, Expressing, Marker, Two Tailed Test

    mc 1r  (Thermo Fisher)


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    Thermo Fisher mc 1r
    Schematic illustration of the structure of Dex/PFP@LIPs-BMS-α, and the theranostic functions of Dex/PFP@LIPs-BMS-α. Liposomes (LIPs) were the main carrier of Dex/PFP@LIPs-BMS-α, which was internally loaded with PFP and Dex, and externally connected with BMS-α by polyethylene glycol (PEG). Glucocorticoid nano-delivery systems were injected <t>into</t> <t>PHN</t> rats through tail vein, which could specifically identify and target binding of <t>MC-1R.</t> Then, the target organ was irradiated by LIFU to fully delivery and release Dex to against PHN.
    Mc 1r, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mc 1r/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mc 1r - by Bioz Stars, 2023-02
    86/100 stars

    Images

    1) Product Images from "Visualized podocyte-targeting and focused ultrasound responsive glucocorticoid nano-delivery system against immune-associated nephropathy without glucocorticoid side effect"

    Article Title: Visualized podocyte-targeting and focused ultrasound responsive glucocorticoid nano-delivery system against immune-associated nephropathy without glucocorticoid side effect

    Journal: Theranostics

    doi: 10.7150/thno.53083

    Schematic illustration of the structure of Dex/PFP@LIPs-BMS-α, and the theranostic functions of Dex/PFP@LIPs-BMS-α. Liposomes (LIPs) were the main carrier of Dex/PFP@LIPs-BMS-α, which was internally loaded with PFP and Dex, and externally connected with BMS-α by polyethylene glycol (PEG). Glucocorticoid nano-delivery systems were injected into PHN rats through tail vein, which could specifically identify and target binding of MC-1R. Then, the target organ was irradiated by LIFU to fully delivery and release Dex to against PHN.
    Figure Legend Snippet: Schematic illustration of the structure of Dex/PFP@LIPs-BMS-α, and the theranostic functions of Dex/PFP@LIPs-BMS-α. Liposomes (LIPs) were the main carrier of Dex/PFP@LIPs-BMS-α, which was internally loaded with PFP and Dex, and externally connected with BMS-α by polyethylene glycol (PEG). Glucocorticoid nano-delivery systems were injected into PHN rats through tail vein, which could specifically identify and target binding of MC-1R. Then, the target organ was irradiated by LIFU to fully delivery and release Dex to against PHN.

    Techniques Used: Injection, Binding Assay, Irradiation

    mc1r  (Thermo Fisher)


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    Thermo Fisher mc1r
    The expression of <t>MC1R,</t> SIRT1 and PGC-1α. (A) Representative western blot images and quantitative analyses of SIRT1 and PGC-1α of the time course study in samples obtained from the left hemisphere after SAH; n = 6 for each group. Data were shown as means ± SD, and were compared by 1-way ANOVA followed by the Tukey post-hoc test. * p < 0.05 vs. sham; (B) Representative microphotographs of immune-fluorescence staining, showing colocalization of MC1R (green) with NeuN, iba-1, and GFAP (all red) 24 h in the sham and SAH (24 h) groups. (n = 2 for each group). Scale bar = 50 μm.
    Mc1r, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mc1r/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mc1r - by Bioz Stars, 2023-02
    86/100 stars

    Images

    1) Product Images from "Melanocortin 1 receptor attenuates early brain injury following subarachnoid hemorrhage by controlling mitochondrial metabolism via AMPK/SIRT1/PGC-1α pathway in rats"

    Article Title: Melanocortin 1 receptor attenuates early brain injury following subarachnoid hemorrhage by controlling mitochondrial metabolism via AMPK/SIRT1/PGC-1α pathway in rats

    Journal: Theranostics

    doi: 10.7150/thno.49426

    The expression of MC1R, SIRT1 and PGC-1α. (A) Representative western blot images and quantitative analyses of SIRT1 and PGC-1α of the time course study in samples obtained from the left hemisphere after SAH; n = 6 for each group. Data were shown as means ± SD, and were compared by 1-way ANOVA followed by the Tukey post-hoc test. * p < 0.05 vs. sham; (B) Representative microphotographs of immune-fluorescence staining, showing colocalization of MC1R (green) with NeuN, iba-1, and GFAP (all red) 24 h in the sham and SAH (24 h) groups. (n = 2 for each group). Scale bar = 50 μm.
    Figure Legend Snippet: The expression of MC1R, SIRT1 and PGC-1α. (A) Representative western blot images and quantitative analyses of SIRT1 and PGC-1α of the time course study in samples obtained from the left hemisphere after SAH; n = 6 for each group. Data were shown as means ± SD, and were compared by 1-way ANOVA followed by the Tukey post-hoc test. * p < 0.05 vs. sham; (B) Representative microphotographs of immune-fluorescence staining, showing colocalization of MC1R (green) with NeuN, iba-1, and GFAP (all red) 24 h in the sham and SAH (24 h) groups. (n = 2 for each group). Scale bar = 50 μm.

    Techniques Used: Expressing, Western Blot, Fluorescence, Staining

    Inhibition of MC1R with MSG-606 abolished the neuroprotective effects of BMS-470539 at 24 h after SAH. (A) Representative western blot images; (B) Quantitative analyses of MC1R, p-AMPK, SIRT1, PGC-1α, UCP2, GPx, SOD, CC-3, Bcl-2, Bax; (C) ROS and ATP levels at 24 h after SAH. n = 6 for each group. Data were shown as means ± SD and compared by 1-way ANOVA followed by the Tukey post-hoc test. * p < 0.05 vs. sham, & p < 0.05 vs. SAH + vehicle, # p < 0.05 vs. SAH + BMS-470539.
    Figure Legend Snippet: Inhibition of MC1R with MSG-606 abolished the neuroprotective effects of BMS-470539 at 24 h after SAH. (A) Representative western blot images; (B) Quantitative analyses of MC1R, p-AMPK, SIRT1, PGC-1α, UCP2, GPx, SOD, CC-3, Bcl-2, Bax; (C) ROS and ATP levels at 24 h after SAH. n = 6 for each group. Data were shown as means ± SD and compared by 1-way ANOVA followed by the Tukey post-hoc test. * p < 0.05 vs. sham, & p < 0.05 vs. SAH + vehicle, # p < 0.05 vs. SAH + BMS-470539.

    Techniques Used: Inhibition, Western Blot

    SIRT1 and PGC-1α were involved in MC1R-mediated neuroprotection at 24 h after SAH. Representative images and quantification of immunofluorescence and Golgi staining of (A, B) DHE; (C, D) FJC; (E, F) TUNEL; (G, H) Golgi. n = 4 for each group. Scale bar = 50 μm. Data were shown as means ± SD and compared by 1-way ANOVA, followed by the Tukey post-hoc test. * p < 0.05 vs. sham, & p < 0.05 vs. SAH + vehicle, # p < 0.05 vs. SAH + BMS-470539.
    Figure Legend Snippet: SIRT1 and PGC-1α were involved in MC1R-mediated neuroprotection at 24 h after SAH. Representative images and quantification of immunofluorescence and Golgi staining of (A, B) DHE; (C, D) FJC; (E, F) TUNEL; (G, H) Golgi. n = 4 for each group. Scale bar = 50 μm. Data were shown as means ± SD and compared by 1-way ANOVA, followed by the Tukey post-hoc test. * p < 0.05 vs. sham, & p < 0.05 vs. SAH + vehicle, # p < 0.05 vs. SAH + BMS-470539.

    Techniques Used: Immunofluorescence, Staining, TUNEL Assay

    mc1r  (Thermo Fisher)


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    Thermo Fisher mc1r
    The expression of <t>MC1R,</t> SIRT1 and PGC-1α. (A) Representative western blot images and quantitative analyses of SIRT1 and PGC-1α of the time course study in samples obtained from the left hemisphere after SAH; n = 6 for each group. Data were shown as means ± SD, and were compared by 1-way ANOVA followed by the Tukey post-hoc test. * p < 0.05 vs. sham; (B) Representative microphotographs of immune-fluorescence staining, showing colocalization of MC1R (green) with NeuN, iba-1, and GFAP (all red) 24 h in the sham and SAH (24 h) groups. (n = 2 for each group). Scale bar = 50 μm.
    Mc1r, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mc1r/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mc1r - by Bioz Stars, 2023-02
    86/100 stars

    Images

    1) Product Images from "Melanocortin 1 receptor attenuates early brain injury following subarachnoid hemorrhage by controlling mitochondrial metabolism via AMPK/SIRT1/PGC-1α pathway in rats"

    Article Title: Melanocortin 1 receptor attenuates early brain injury following subarachnoid hemorrhage by controlling mitochondrial metabolism via AMPK/SIRT1/PGC-1α pathway in rats

    Journal: Theranostics

    doi: 10.7150/thno.49426

    The expression of MC1R, SIRT1 and PGC-1α. (A) Representative western blot images and quantitative analyses of SIRT1 and PGC-1α of the time course study in samples obtained from the left hemisphere after SAH; n = 6 for each group. Data were shown as means ± SD, and were compared by 1-way ANOVA followed by the Tukey post-hoc test. * p < 0.05 vs. sham; (B) Representative microphotographs of immune-fluorescence staining, showing colocalization of MC1R (green) with NeuN, iba-1, and GFAP (all red) 24 h in the sham and SAH (24 h) groups. (n = 2 for each group). Scale bar = 50 μm.
    Figure Legend Snippet: The expression of MC1R, SIRT1 and PGC-1α. (A) Representative western blot images and quantitative analyses of SIRT1 and PGC-1α of the time course study in samples obtained from the left hemisphere after SAH; n = 6 for each group. Data were shown as means ± SD, and were compared by 1-way ANOVA followed by the Tukey post-hoc test. * p < 0.05 vs. sham; (B) Representative microphotographs of immune-fluorescence staining, showing colocalization of MC1R (green) with NeuN, iba-1, and GFAP (all red) 24 h in the sham and SAH (24 h) groups. (n = 2 for each group). Scale bar = 50 μm.

    Techniques Used: Expressing, Western Blot, Fluorescence, Staining

    Inhibition of MC1R with MSG-606 abolished the neuroprotective effects of BMS-470539 at 24 h after SAH. (A) Representative western blot images; (B) Quantitative analyses of MC1R, p-AMPK, SIRT1, PGC-1α, UCP2, GPx, SOD, CC-3, Bcl-2, Bax; (C) ROS and ATP levels at 24 h after SAH. n = 6 for each group. Data were shown as means ± SD and compared by 1-way ANOVA followed by the Tukey post-hoc test. * p < 0.05 vs. sham, & p < 0.05 vs. SAH + vehicle, # p < 0.05 vs. SAH + BMS-470539.
    Figure Legend Snippet: Inhibition of MC1R with MSG-606 abolished the neuroprotective effects of BMS-470539 at 24 h after SAH. (A) Representative western blot images; (B) Quantitative analyses of MC1R, p-AMPK, SIRT1, PGC-1α, UCP2, GPx, SOD, CC-3, Bcl-2, Bax; (C) ROS and ATP levels at 24 h after SAH. n = 6 for each group. Data were shown as means ± SD and compared by 1-way ANOVA followed by the Tukey post-hoc test. * p < 0.05 vs. sham, & p < 0.05 vs. SAH + vehicle, # p < 0.05 vs. SAH + BMS-470539.

    Techniques Used: Inhibition, Western Blot

    SIRT1 and PGC-1α were involved in MC1R-mediated neuroprotection at 24 h after SAH. Representative images and quantification of immunofluorescence and Golgi staining of (A, B) DHE; (C, D) FJC; (E, F) TUNEL; (G, H) Golgi. n = 4 for each group. Scale bar = 50 μm. Data were shown as means ± SD and compared by 1-way ANOVA, followed by the Tukey post-hoc test. * p < 0.05 vs. sham, & p < 0.05 vs. SAH + vehicle, # p < 0.05 vs. SAH + BMS-470539.
    Figure Legend Snippet: SIRT1 and PGC-1α were involved in MC1R-mediated neuroprotection at 24 h after SAH. Representative images and quantification of immunofluorescence and Golgi staining of (A, B) DHE; (C, D) FJC; (E, F) TUNEL; (G, H) Golgi. n = 4 for each group. Scale bar = 50 μm. Data were shown as means ± SD and compared by 1-way ANOVA, followed by the Tukey post-hoc test. * p < 0.05 vs. sham, & p < 0.05 vs. SAH + vehicle, # p < 0.05 vs. SAH + BMS-470539.

    Techniques Used: Immunofluorescence, Staining, TUNEL Assay

    gene exp tubb3 hs00801390 s1  (Thermo Fisher)


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    Thermo Fisher gene exp tubb3 hs00801390 s1
    Gene Exp Tubb3 Hs00801390 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp tubb3 hs00801390 s1/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gene exp tubb3 hs00801390 s1 - by Bioz Stars, 2023-02
    99/100 stars

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    gene exp tubb3 hs00801390 s1  (Thermo Fisher)


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    Thermo Fisher gene exp tubb3 hs00801390 s1
    Gene Exp Tubb3 Hs00801390 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp tubb3 hs00801390 s1/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gene exp tubb3 hs00801390 s1 - by Bioz Stars, 2023-02
    99/100 stars

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    gene exp tubb3 hs00801390 s1  (Thermo Fisher)


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    Thermo Fisher gene exp tubb3 hs00801390 s1
    qRT-PCR analysis. Notes: Unlabeled and labeled iPSC-NPs with CZF and PLL-coated γ-Fe 2 O 3 were analyzed 2 weeks after the onset of differentiation. ( A ) The expression of the neural genes NES, <t>TUBB3,</t> SYP, and GFAP. ( B ) The expression of the mDN genes FOXA2 , EN1 , NURR1 and TH (* P <0.05). The average expression of studied markers in the unlabeled undifferentiated control was set as zero. Abbreviations: qRT-PCR, quantitative real-time reverse transcription polymerase chain reaction; iPSC-NPs, induced pluripotent stem cell-derived neural precursors; CZF, silica-coated cobalt zinc ferrite nanoparticles; PLL-coated γ-Fe 2 O 3 , poly- l -lysine-coated iron oxide superparamagnetic nanoparticles; SYP, synaptophysin; GFAP, glial fibrillary acidic protein; mDN, midbrain dopaminergic neuron.
    Gene Exp Tubb3 Hs00801390 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp tubb3 hs00801390 s1/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gene exp tubb3 hs00801390 s1 - by Bioz Stars, 2023-02
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    Images

    1) Product Images from "The effect of magnetic nanoparticles on neuronal differentiation of induced pluripotent stem cell-derived neural precursors"

    Article Title: The effect of magnetic nanoparticles on neuronal differentiation of induced pluripotent stem cell-derived neural precursors

    Journal: International Journal of Nanomedicine

    doi: 10.2147/IJN.S116171

    qRT-PCR analysis. Notes: Unlabeled and labeled iPSC-NPs with CZF and PLL-coated γ-Fe 2 O 3 were analyzed 2 weeks after the onset of differentiation. ( A ) The expression of the neural genes NES, TUBB3, SYP, and GFAP. ( B ) The expression of the mDN genes FOXA2 , EN1 , NURR1 and TH (* P <0.05). The average expression of studied markers in the unlabeled undifferentiated control was set as zero. Abbreviations: qRT-PCR, quantitative real-time reverse transcription polymerase chain reaction; iPSC-NPs, induced pluripotent stem cell-derived neural precursors; CZF, silica-coated cobalt zinc ferrite nanoparticles; PLL-coated γ-Fe 2 O 3 , poly- l -lysine-coated iron oxide superparamagnetic nanoparticles; SYP, synaptophysin; GFAP, glial fibrillary acidic protein; mDN, midbrain dopaminergic neuron.
    Figure Legend Snippet: qRT-PCR analysis. Notes: Unlabeled and labeled iPSC-NPs with CZF and PLL-coated γ-Fe 2 O 3 were analyzed 2 weeks after the onset of differentiation. ( A ) The expression of the neural genes NES, TUBB3, SYP, and GFAP. ( B ) The expression of the mDN genes FOXA2 , EN1 , NURR1 and TH (* P <0.05). The average expression of studied markers in the unlabeled undifferentiated control was set as zero. Abbreviations: qRT-PCR, quantitative real-time reverse transcription polymerase chain reaction; iPSC-NPs, induced pluripotent stem cell-derived neural precursors; CZF, silica-coated cobalt zinc ferrite nanoparticles; PLL-coated γ-Fe 2 O 3 , poly- l -lysine-coated iron oxide superparamagnetic nanoparticles; SYP, synaptophysin; GFAP, glial fibrillary acidic protein; mDN, midbrain dopaminergic neuron.

    Techniques Used: Quantitative RT-PCR, Labeling, Expressing, Reverse Transcription Polymerase Chain Reaction, Derivative Assay

    tubb3 sirna  (Thermo Fisher)


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    Thermo Fisher tubb3 sirna
    Expression and functional analysis of MDR1. (a, b) The expression of <t>TUBB3</t> was investigated by real-time quantitative PCR and Western blotting in each cell line. Values represent mean ± SD. ∗ P < 0.05, versus parental cell line. (c) Functional activity of MDR1 was analyzed by EFLUXX-ID Green Multidrug Resistance Assay. The left black curve shows unstained cells as a negative control. The right blue curve shows cells stained with EFLUXX-ID Green.
    Tubb3 Sirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tubb3 sirna/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tubb3 sirna - by Bioz Stars, 2023-02
    86/100 stars

    Images

    1) Product Images from "Class III β -Tubulin Overexpression Induces Chemoresistance to Eribulin in a Leiomyosarcoma Cell Line"

    Article Title: Class III β -Tubulin Overexpression Induces Chemoresistance to Eribulin in a Leiomyosarcoma Cell Line

    Journal: Analytical Cellular Pathology (Amsterdam)

    doi: 10.1155/2018/8987568

    Expression and functional analysis of MDR1. (a, b) The expression of TUBB3 was investigated by real-time quantitative PCR and Western blotting in each cell line. Values represent mean ± SD. ∗ P < 0.05, versus parental cell line. (c) Functional activity of MDR1 was analyzed by EFLUXX-ID Green Multidrug Resistance Assay. The left black curve shows unstained cells as a negative control. The right blue curve shows cells stained with EFLUXX-ID Green.
    Figure Legend Snippet: Expression and functional analysis of MDR1. (a, b) The expression of TUBB3 was investigated by real-time quantitative PCR and Western blotting in each cell line. Values represent mean ± SD. ∗ P < 0.05, versus parental cell line. (c) Functional activity of MDR1 was analyzed by EFLUXX-ID Green Multidrug Resistance Assay. The left black curve shows unstained cells as a negative control. The right blue curve shows cells stained with EFLUXX-ID Green.

    Techniques Used: Expressing, Functional Assay, Real-time Polymerase Chain Reaction, Western Blot, Activity Assay, Negative Control, Staining

    β -Tubulin profile and TUBB3 knockdown in parental and eribulin-resistant cell lines. (a) The β -tubulin profile (TUBB1, TUBB3, TUBB4, and TUBB5) was investigated by real-time quantitative PCR in each cell line. Values represent mean ± SD. ∗ P < 0.05, versus parental cell line. (b) Overexpression of TUBB3 was confirmed by Western blotting. GAPDH was used for internal normalization. (c) The expression of TUBB3 after transfection of siRNA was analyzed by real-time quantitative PCR and Western blotting. Values represent mean ± SD. ∗ P < 0.05. (d) The sensitivity to eribulin after transfection of siRNA was assessed by determination of cell viability and cell cycle analysis. Left panel: the cell viability assay was performed after transfection of siRNA and 48-hour incubation with eribulin. Right panel: cell cycle analysis was performed after transfection of siRNA and 12-hour incubation with eribulin. Values represent mean ± SD. ∗ P < 0.05, versus resistant cell line transfected with siControl. (e) Cell cycle analysis of the resistant cell line 4 weeks after incubation without eribulin. The left panel represents analysis of the resistant cell line. The right panel represents analysis of the revertant cell line which was incubated without eribulin for 4 weeks. There was no significant difference in each cell cycle, so chemoresistance to eribulin was maintained 4 weeks after incubation without eribulin. (f) The doubling time of three cell lines, parental, resistant, and revertant, was measured by using CellTiter-Glo. The solid line represents the parental cell line, the dotted line represents the resistant cell line, and the broken line represents revertant used in . Values represent mean ± SD.
    Figure Legend Snippet: β -Tubulin profile and TUBB3 knockdown in parental and eribulin-resistant cell lines. (a) The β -tubulin profile (TUBB1, TUBB3, TUBB4, and TUBB5) was investigated by real-time quantitative PCR in each cell line. Values represent mean ± SD. ∗ P < 0.05, versus parental cell line. (b) Overexpression of TUBB3 was confirmed by Western blotting. GAPDH was used for internal normalization. (c) The expression of TUBB3 after transfection of siRNA was analyzed by real-time quantitative PCR and Western blotting. Values represent mean ± SD. ∗ P < 0.05. (d) The sensitivity to eribulin after transfection of siRNA was assessed by determination of cell viability and cell cycle analysis. Left panel: the cell viability assay was performed after transfection of siRNA and 48-hour incubation with eribulin. Right panel: cell cycle analysis was performed after transfection of siRNA and 12-hour incubation with eribulin. Values represent mean ± SD. ∗ P < 0.05, versus resistant cell line transfected with siControl. (e) Cell cycle analysis of the resistant cell line 4 weeks after incubation without eribulin. The left panel represents analysis of the resistant cell line. The right panel represents analysis of the revertant cell line which was incubated without eribulin for 4 weeks. There was no significant difference in each cell cycle, so chemoresistance to eribulin was maintained 4 weeks after incubation without eribulin. (f) The doubling time of three cell lines, parental, resistant, and revertant, was measured by using CellTiter-Glo. The solid line represents the parental cell line, the dotted line represents the resistant cell line, and the broken line represents revertant used in . Values represent mean ± SD.

    Techniques Used: Real-time Polymerase Chain Reaction, Over Expression, Western Blot, Expressing, Transfection, Cell Cycle Assay, Viability Assay, Incubation

    Expression of TUBB3 in clinical leiomyosarcoma samples. (a) Immunohistochemical staining of TUBB3 in 68 clinical samples from patients with leiomyosarcoma. (A, B) the representative samples of immunohistochemical staining of TUBB3: (A) low TUBB3 expression and (B) high TUBB3 expression. Scale bar represents 100 μ m in (A) and (B). (b) Overall survival analysis of the patients with leiomyosarcoma which express high or low TUBB3 by the Kaplan-Meier method. The solid line represents high TUBB3 group, and the dotted line represents low TUBB3 group. ∗ P < 0.05. (c) Analysis of TUBB3 expression and MIB-1 positive ratio (%). The high TUBB3 group exhibited a significantly higher MIB-1 positive ratio than did the low TUBB3 group. ∗ P < 0.05. (d) HE and immunohistochemical staining of TUBB3 in tumor biopsies from patients who were treated with eribulin. (A, B, C) Upper specimens represent the tissue samples from the patient who continued to have stable disease (SD) 12 weeks after initial treatment with eribulin. (D, E, F) Lower specimens represent the tissue samples from the patient who resulted in progressive disease (PD) after treatment with eribulin. (A, D) Staining with hematoxylin and eosin. (B, C, E, F) Immunohistochemical staining of TUBB3. Scale bar represents 100 μ m in (A) and (D), 200 μ m in (B) and (E), and 20 μ m in (C) and (F).
    Figure Legend Snippet: Expression of TUBB3 in clinical leiomyosarcoma samples. (a) Immunohistochemical staining of TUBB3 in 68 clinical samples from patients with leiomyosarcoma. (A, B) the representative samples of immunohistochemical staining of TUBB3: (A) low TUBB3 expression and (B) high TUBB3 expression. Scale bar represents 100 μ m in (A) and (B). (b) Overall survival analysis of the patients with leiomyosarcoma which express high or low TUBB3 by the Kaplan-Meier method. The solid line represents high TUBB3 group, and the dotted line represents low TUBB3 group. ∗ P < 0.05. (c) Analysis of TUBB3 expression and MIB-1 positive ratio (%). The high TUBB3 group exhibited a significantly higher MIB-1 positive ratio than did the low TUBB3 group. ∗ P < 0.05. (d) HE and immunohistochemical staining of TUBB3 in tumor biopsies from patients who were treated with eribulin. (A, B, C) Upper specimens represent the tissue samples from the patient who continued to have stable disease (SD) 12 weeks after initial treatment with eribulin. (D, E, F) Lower specimens represent the tissue samples from the patient who resulted in progressive disease (PD) after treatment with eribulin. (A, D) Staining with hematoxylin and eosin. (B, C, E, F) Immunohistochemical staining of TUBB3. Scale bar represents 100 μ m in (A) and (D), 200 μ m in (B) and (E), and 20 μ m in (C) and (F).

    Techniques Used: Expressing, Immunohistochemical staining, Staining

    gene exp mc1r hs00267167 s1  (Thermo Fisher)


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    Thermo Fisher gene exp mc1r hs00267167 s1
    (A) Five different doses of adriamycin, in the range of 5–10 mg/kg, were intravenously injected and the level of albuminuria was measured after 7 days. The urinary albumin-to-creatinine ratio (UACR) increased with increasing levels of adriamycin. (B) Albuminuria at day 7. Treatment with the <t>MC1R</t> agonist BMS-470539 did not reduce the level of albuminuria for the 8 mg/kg adriamycin dose, on the contrary it slightly increased albuminuria for the 10 mg/kg dose (n = 17–19, p<0.05). The unspecific melanocortin receptor agonist α-MSH did not have any significant effect (n = 9, n.s.) compared with untreated adriamycin mice. Results are presented as mean ± SEM.
    Gene Exp Mc1r Hs00267167 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp mc1r hs00267167 s1/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gene exp mc1r hs00267167 s1 - by Bioz Stars, 2023-02
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    Images

    1) Product Images from "Effects of Melanocortin 1 Receptor Agonists in Experimental Nephropathies"

    Article Title: Effects of Melanocortin 1 Receptor Agonists in Experimental Nephropathies

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0087816

    (A) Five different doses of adriamycin, in the range of 5–10 mg/kg, were intravenously injected and the level of albuminuria was measured after 7 days. The urinary albumin-to-creatinine ratio (UACR) increased with increasing levels of adriamycin. (B) Albuminuria at day 7. Treatment with the MC1R agonist BMS-470539 did not reduce the level of albuminuria for the 8 mg/kg adriamycin dose, on the contrary it slightly increased albuminuria for the 10 mg/kg dose (n = 17–19, p<0.05). The unspecific melanocortin receptor agonist α-MSH did not have any significant effect (n = 9, n.s.) compared with untreated adriamycin mice. Results are presented as mean ± SEM.
    Figure Legend Snippet: (A) Five different doses of adriamycin, in the range of 5–10 mg/kg, were intravenously injected and the level of albuminuria was measured after 7 days. The urinary albumin-to-creatinine ratio (UACR) increased with increasing levels of adriamycin. (B) Albuminuria at day 7. Treatment with the MC1R agonist BMS-470539 did not reduce the level of albuminuria for the 8 mg/kg adriamycin dose, on the contrary it slightly increased albuminuria for the 10 mg/kg dose (n = 17–19, p<0.05). The unspecific melanocortin receptor agonist α-MSH did not have any significant effect (n = 9, n.s.) compared with untreated adriamycin mice. Results are presented as mean ± SEM.

    Techniques Used: Injection

    Gene expression of  MC1R  in human, rat and mouse kidney tissue.
    Figure Legend Snippet: Gene expression of MC1R in human, rat and mouse kidney tissue.

    Techniques Used: Expressing

    Western blotting was performed in order to detect MC1R protein expression in mouse and human tissue. Left panel: incubation with a rabbit polyclonal MC1R antibody (Alomone Labs). Right panel: incubation with MC1R antibody and control blocking peptide antigen (BP) in order to show antibody specificity. A) mouse whole glomerular lysate, B) cultured wild type mouse podocytes and C) a human malignant melanoma cell line A375. MC1R protein expression was detected at the expected size of 37 kDa (left panel).
    Figure Legend Snippet: Western blotting was performed in order to detect MC1R protein expression in mouse and human tissue. Left panel: incubation with a rabbit polyclonal MC1R antibody (Alomone Labs). Right panel: incubation with MC1R antibody and control blocking peptide antigen (BP) in order to show antibody specificity. A) mouse whole glomerular lysate, B) cultured wild type mouse podocytes and C) a human malignant melanoma cell line A375. MC1R protein expression was detected at the expected size of 37 kDa (left panel).

    Techniques Used: Western Blot, Expressing, Incubation, Blocking Assay, Cell Culture

    gene exp mc1r mm00434851 s1  (Thermo Fisher)


    Bioz Verified Symbol Thermo Fisher is a verified supplier
    Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
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  • 85

    Structured Review

    Thermo Fisher gene exp mc1r mm00434851 s1
    (A) Five different doses of adriamycin, in the range of 5–10 mg/kg, were intravenously injected and the level of albuminuria was measured after 7 days. The urinary albumin-to-creatinine ratio (UACR) increased with increasing levels of adriamycin. (B) Albuminuria at day 7. Treatment with the <t>MC1R</t> agonist BMS-470539 did not reduce the level of albuminuria for the 8 mg/kg adriamycin dose, on the contrary it slightly increased albuminuria for the 10 mg/kg dose (n = 17–19, p<0.05). The unspecific melanocortin receptor agonist α-MSH did not have any significant effect (n = 9, n.s.) compared with untreated adriamycin mice. Results are presented as mean ± SEM.
    Gene Exp Mc1r Mm00434851 S1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp mc1r mm00434851 s1/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gene exp mc1r mm00434851 s1 - by Bioz Stars, 2023-02
    85/100 stars

    Images

    1) Product Images from "Effects of Melanocortin 1 Receptor Agonists in Experimental Nephropathies"

    Article Title: Effects of Melanocortin 1 Receptor Agonists in Experimental Nephropathies

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0087816

    (A) Five different doses of adriamycin, in the range of 5–10 mg/kg, were intravenously injected and the level of albuminuria was measured after 7 days. The urinary albumin-to-creatinine ratio (UACR) increased with increasing levels of adriamycin. (B) Albuminuria at day 7. Treatment with the MC1R agonist BMS-470539 did not reduce the level of albuminuria for the 8 mg/kg adriamycin dose, on the contrary it slightly increased albuminuria for the 10 mg/kg dose (n = 17–19, p<0.05). The unspecific melanocortin receptor agonist α-MSH did not have any significant effect (n = 9, n.s.) compared with untreated adriamycin mice. Results are presented as mean ± SEM.
    Figure Legend Snippet: (A) Five different doses of adriamycin, in the range of 5–10 mg/kg, were intravenously injected and the level of albuminuria was measured after 7 days. The urinary albumin-to-creatinine ratio (UACR) increased with increasing levels of adriamycin. (B) Albuminuria at day 7. Treatment with the MC1R agonist BMS-470539 did not reduce the level of albuminuria for the 8 mg/kg adriamycin dose, on the contrary it slightly increased albuminuria for the 10 mg/kg dose (n = 17–19, p<0.05). The unspecific melanocortin receptor agonist α-MSH did not have any significant effect (n = 9, n.s.) compared with untreated adriamycin mice. Results are presented as mean ± SEM.

    Techniques Used: Injection

    Gene expression of  MC1R  in human, rat and mouse kidney tissue.
    Figure Legend Snippet: Gene expression of MC1R in human, rat and mouse kidney tissue.

    Techniques Used: Expressing

    Western blotting was performed in order to detect MC1R protein expression in mouse and human tissue. Left panel: incubation with a rabbit polyclonal MC1R antibody (Alomone Labs). Right panel: incubation with MC1R antibody and control blocking peptide antigen (BP) in order to show antibody specificity. A) mouse whole glomerular lysate, B) cultured wild type mouse podocytes and C) a human malignant melanoma cell line A375. MC1R protein expression was detected at the expected size of 37 kDa (left panel).
    Figure Legend Snippet: Western blotting was performed in order to detect MC1R protein expression in mouse and human tissue. Left panel: incubation with a rabbit polyclonal MC1R antibody (Alomone Labs). Right panel: incubation with MC1R antibody and control blocking peptide antigen (BP) in order to show antibody specificity. A) mouse whole glomerular lysate, B) cultured wild type mouse podocytes and C) a human malignant melanoma cell line A375. MC1R protein expression was detected at the expected size of 37 kDa (left panel).

    Techniques Used: Western Blot, Expressing, Incubation, Blocking Assay, Cell Culture