recombinant dnase i  (Thermo Fisher)


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    Structured Review

    Thermo Fisher recombinant dnase i
    Recombinant Dnase I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant dnase i/product/Thermo Fisher
    Average 99 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    recombinant dnase i - by Bioz Stars, 2020-03
    99/100 stars

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    Related Articles

    TUNEL Assay:

    Article Title: Granzyme B-induced and Caspase 3-dependent Cleavage of Gelsolin by Mouse Cytotoxic T Cells Modifies Cytoskeleton Dynamics *
    Article Snippet: .. For the negative or positive control, the fixed and permeabilized cells were incubated without the terminal deoxynucleotidyltransferase instead of the TUNEL (terminal deoxyribonucleotidyltransferase-mediated dUTP nick end labeling) reaction mixture or with 1 unit of rec.DNase I (Invitrogen) at room temperature for 10 min before to labeling procedures, respectively. .. Analyses using FACS were done as previously described ( , ) using fluorescein isothiocyanate- and allophycocyanin-labeled anti-CD8a mAb (clone 53-6.7; BD Pharmingen), fluorescein isothiocyanate-labeled anti-Fas mAb (clone Jo2; BD Pharmingen) for surface staining diluted in an anti-Fc receptor mAb (clone 2.4G2) and anti-nat.gzmA IgG , anti-rec.gzmB IgG , anti-human caspase 3 mAb fluorescein isothiocyanate (clone C92–605; BD Pharmingen), and anti-rec.c-gelsolin IS for intracellular staining diluted in permeabilizing buffer (phosphate-buffered saline, 5% fetal calf serum, 0.1% NaN3 , 0.1% saponin (Roth)).

    RNA Extraction:

    Article Title: Elevated expression of catechol-o-methyltransferase is associated with labor and increased PGE2 production by human fetal membranes
    Article Snippet: Paragraph title: RNA extraction ... All RNA samples were treated with a recombinant DNase I (Ambion; Austin, TX) to remove contaminating DNA.

    Fluorescence:

    Article Title: Granzyme B-induced and Caspase 3-dependent Cleavage of Gelsolin by Mouse Cytotoxic T Cells Modifies Cytoskeleton Dynamics *
    Article Snippet: Paragraph title: Staining for Fluorescence Microscopy ... For the negative or positive control, the fixed and permeabilized cells were incubated without the terminal deoxynucleotidyltransferase instead of the TUNEL (terminal deoxyribonucleotidyltransferase-mediated dUTP nick end labeling) reaction mixture or with 1 unit of rec.DNase I (Invitrogen) at room temperature for 10 min before to labeling procedures, respectively.

    Positive Control:

    Article Title: Granzyme B-induced and Caspase 3-dependent Cleavage of Gelsolin by Mouse Cytotoxic T Cells Modifies Cytoskeleton Dynamics *
    Article Snippet: .. For the negative or positive control, the fixed and permeabilized cells were incubated without the terminal deoxynucleotidyltransferase instead of the TUNEL (terminal deoxyribonucleotidyltransferase-mediated dUTP nick end labeling) reaction mixture or with 1 unit of rec.DNase I (Invitrogen) at room temperature for 10 min before to labeling procedures, respectively. .. Analyses using FACS were done as previously described ( , ) using fluorescein isothiocyanate- and allophycocyanin-labeled anti-CD8a mAb (clone 53-6.7; BD Pharmingen), fluorescein isothiocyanate-labeled anti-Fas mAb (clone Jo2; BD Pharmingen) for surface staining diluted in an anti-Fc receptor mAb (clone 2.4G2) and anti-nat.gzmA IgG , anti-rec.gzmB IgG , anti-human caspase 3 mAb fluorescein isothiocyanate (clone C92–605; BD Pharmingen), and anti-rec.c-gelsolin IS for intracellular staining diluted in permeabilizing buffer (phosphate-buffered saline, 5% fetal calf serum, 0.1% NaN3 , 0.1% saponin (Roth)).

    In Situ:

    Article Title: Granzyme B-induced and Caspase 3-dependent Cleavage of Gelsolin by Mouse Cytotoxic T Cells Modifies Cytoskeleton Dynamics *
    Article Snippet: For staining with the in situ cell death detection kit TMR red, MC.Gelsolin−/− cells were treated and stained with anti-rec.c-gelsolin IS and anti-rabbit IgG Alexa488 as described above followed by staining with the in situ cell death detection kit TMR red according to manufacturer's instructions. .. For the negative or positive control, the fixed and permeabilized cells were incubated without the terminal deoxynucleotidyltransferase instead of the TUNEL (terminal deoxyribonucleotidyltransferase-mediated dUTP nick end labeling) reaction mixture or with 1 unit of rec.DNase I (Invitrogen) at room temperature for 10 min before to labeling procedures, respectively.

    Labeling:

    Article Title: Granzyme B-induced and Caspase 3-dependent Cleavage of Gelsolin by Mouse Cytotoxic T Cells Modifies Cytoskeleton Dynamics *
    Article Snippet: .. For the negative or positive control, the fixed and permeabilized cells were incubated without the terminal deoxynucleotidyltransferase instead of the TUNEL (terminal deoxyribonucleotidyltransferase-mediated dUTP nick end labeling) reaction mixture or with 1 unit of rec.DNase I (Invitrogen) at room temperature for 10 min before to labeling procedures, respectively. .. Analyses using FACS were done as previously described ( , ) using fluorescein isothiocyanate- and allophycocyanin-labeled anti-CD8a mAb (clone 53-6.7; BD Pharmingen), fluorescein isothiocyanate-labeled anti-Fas mAb (clone Jo2; BD Pharmingen) for surface staining diluted in an anti-Fc receptor mAb (clone 2.4G2) and anti-nat.gzmA IgG , anti-rec.gzmB IgG , anti-human caspase 3 mAb fluorescein isothiocyanate (clone C92–605; BD Pharmingen), and anti-rec.c-gelsolin IS for intracellular staining diluted in permeabilizing buffer (phosphate-buffered saline, 5% fetal calf serum, 0.1% NaN3 , 0.1% saponin (Roth)).

    Incubation:

    Article Title: Granzyme B-induced and Caspase 3-dependent Cleavage of Gelsolin by Mouse Cytotoxic T Cells Modifies Cytoskeleton Dynamics *
    Article Snippet: .. For the negative or positive control, the fixed and permeabilized cells were incubated without the terminal deoxynucleotidyltransferase instead of the TUNEL (terminal deoxyribonucleotidyltransferase-mediated dUTP nick end labeling) reaction mixture or with 1 unit of rec.DNase I (Invitrogen) at room temperature for 10 min before to labeling procedures, respectively. .. Analyses using FACS were done as previously described ( , ) using fluorescein isothiocyanate- and allophycocyanin-labeled anti-CD8a mAb (clone 53-6.7; BD Pharmingen), fluorescein isothiocyanate-labeled anti-Fas mAb (clone Jo2; BD Pharmingen) for surface staining diluted in an anti-Fc receptor mAb (clone 2.4G2) and anti-nat.gzmA IgG , anti-rec.gzmB IgG , anti-human caspase 3 mAb fluorescein isothiocyanate (clone C92–605; BD Pharmingen), and anti-rec.c-gelsolin IS for intracellular staining diluted in permeabilizing buffer (phosphate-buffered saline, 5% fetal calf serum, 0.1% NaN3 , 0.1% saponin (Roth)).

    Microscopy:

    Article Title: Granzyme B-induced and Caspase 3-dependent Cleavage of Gelsolin by Mouse Cytotoxic T Cells Modifies Cytoskeleton Dynamics *
    Article Snippet: Paragraph title: Staining for Fluorescence Microscopy ... For the negative or positive control, the fixed and permeabilized cells were incubated without the terminal deoxynucleotidyltransferase instead of the TUNEL (terminal deoxyribonucleotidyltransferase-mediated dUTP nick end labeling) reaction mixture or with 1 unit of rec.DNase I (Invitrogen) at room temperature for 10 min before to labeling procedures, respectively.

    End Labeling:

    Article Title: Granzyme B-induced and Caspase 3-dependent Cleavage of Gelsolin by Mouse Cytotoxic T Cells Modifies Cytoskeleton Dynamics *
    Article Snippet: .. For the negative or positive control, the fixed and permeabilized cells were incubated without the terminal deoxynucleotidyltransferase instead of the TUNEL (terminal deoxyribonucleotidyltransferase-mediated dUTP nick end labeling) reaction mixture or with 1 unit of rec.DNase I (Invitrogen) at room temperature for 10 min before to labeling procedures, respectively. .. Analyses using FACS were done as previously described ( , ) using fluorescein isothiocyanate- and allophycocyanin-labeled anti-CD8a mAb (clone 53-6.7; BD Pharmingen), fluorescein isothiocyanate-labeled anti-Fas mAb (clone Jo2; BD Pharmingen) for surface staining diluted in an anti-Fc receptor mAb (clone 2.4G2) and anti-nat.gzmA IgG , anti-rec.gzmB IgG , anti-human caspase 3 mAb fluorescein isothiocyanate (clone C92–605; BD Pharmingen), and anti-rec.c-gelsolin IS for intracellular staining diluted in permeabilizing buffer (phosphate-buffered saline, 5% fetal calf serum, 0.1% NaN3 , 0.1% saponin (Roth)).

    Staining:

    Article Title: Granzyme B-induced and Caspase 3-dependent Cleavage of Gelsolin by Mouse Cytotoxic T Cells Modifies Cytoskeleton Dynamics *
    Article Snippet: Paragraph title: Staining for Fluorescence Microscopy ... For the negative or positive control, the fixed and permeabilized cells were incubated without the terminal deoxynucleotidyltransferase instead of the TUNEL (terminal deoxyribonucleotidyltransferase-mediated dUTP nick end labeling) reaction mixture or with 1 unit of rec.DNase I (Invitrogen) at room temperature for 10 min before to labeling procedures, respectively.

    Recombinant:

    Article Title: Elevated expression of catechol-o-methyltransferase is associated with labor and increased PGE2 production by human fetal membranes
    Article Snippet: .. All RNA samples were treated with a recombinant DNase I (Ambion; Austin, TX) to remove contaminating DNA. ..

    Software:

    Article Title: Granzyme B-induced and Caspase 3-dependent Cleavage of Gelsolin by Mouse Cytotoxic T Cells Modifies Cytoskeleton Dynamics *
    Article Snippet: The cells were stained with anti-rec.c-gelsolin IS diluted in saponin buffer/1× Roti-Block (Roth) at room temperature for 45 min followed by incubation with an anti-rabbit IgG Alexa 488 (Invitrogen) or phalloidin Alexa Fluor 546 (Invitrogen) diluted in saponin buffer/1× Roti-Block at room temperature for 45 min. After washing, coverslips were embedded with Fluoromount-G containing Hoechst 33342 (10 μg/ml; Invitrogen) and analyzed by fluorescence microscopy using a Zeiss microscope (Imager.Z1 Axio with Axiocam; Zeiss) and Zeiss AxioVision Rel 4.7.2 as software. .. For the negative or positive control, the fixed and permeabilized cells were incubated without the terminal deoxynucleotidyltransferase instead of the TUNEL (terminal deoxyribonucleotidyltransferase-mediated dUTP nick end labeling) reaction mixture or with 1 unit of rec.DNase I (Invitrogen) at room temperature for 10 min before to labeling procedures, respectively.

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