real peroxidase blocking solution  (Agilent technologies)

 
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    Name:
    Dako Omnis
    Description:
    Designed for true patient case management the Dako Omnis solution enables you to run IHC IF FISH and CISH simultaneously each with a unique combination of reagents Combined with an onboard capacity of 60 slides and 60 temperature controlled reagent positions this gives you a flexible and lean workflow Dako Omnis has an unmatched throughput in a typical workday of 165 IHC or 30 ISH slides in addition to unparalleled same day results with turnaround times of just 2 5 hours for IHC 4 hours for FISH and 5 hours for CISH Dako Omnis provides your lab more time greater choice and better patient care with consistent high quality staining
    Catalog Number:
    DAKO-OMNIS
    Price:
    None
    Category:
    Products Dako Omnis Solution For Ihc Ish Dako Omnis Dako Omnis
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    Structured Review

    Agilent technologies real peroxidase blocking solution
    Designed for true patient case management the Dako Omnis solution enables you to run IHC IF FISH and CISH simultaneously each with a unique combination of reagents Combined with an onboard capacity of 60 slides and 60 temperature controlled reagent positions this gives you a flexible and lean workflow Dako Omnis has an unmatched throughput in a typical workday of 165 IHC or 30 ISH slides in addition to unparalleled same day results with turnaround times of just 2 5 hours for IHC 4 hours for FISH and 5 hours for CISH Dako Omnis provides your lab more time greater choice and better patient care with consistent high quality staining
    https://www.bioz.com/result/real peroxidase blocking solution/product/Agilent technologies
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    real peroxidase blocking solution - by Bioz Stars, 2021-04
    97/100 stars

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    Related Articles

    Activity Assay:

    Article Title: Increased Soluble Epoxide Hydrolase in Human Gestational Tissues from Pregnancies Complicated by Acute Chorioamnionitis
    Article Snippet: Immunohistochemistry Immunohistochemistry for sEH, cytokeratin 7 (CK7), and CD68 was performed as previously described [ ]. .. Briefly, after quenching endogenous peroxidase activity and blocking nonspecific binding, sections were reacted with mouse anti-human sEH (A-5) monoclonal antibody (1 : 10 dilution; catalog no. SC-166961; Santa Cruz Biotechnology, Inc. Dallas, TX, USA), anti-human CK7 monoclonal antibody (1 : 1000 dilution, clone OV-TL 12/30, Dako Omnis, Agilent, Santa Clara, CA, USA), or anti-human CD68 monoclonal antibody (1 : 1000 dilution, clone KP1, Dako Omnis) at 4°C overnight. .. Further processing for colorimetric detection was according to the instructions for the VECTASTAIN Elite ABC Kit (Vector Laboratories, Burlingame, CA, USA) using diaminobenzidine as the peroxidase substrate.

    Blocking Assay:

    Article Title: Increased Soluble Epoxide Hydrolase in Human Gestational Tissues from Pregnancies Complicated by Acute Chorioamnionitis
    Article Snippet: Immunohistochemistry Immunohistochemistry for sEH, cytokeratin 7 (CK7), and CD68 was performed as previously described [ ]. .. Briefly, after quenching endogenous peroxidase activity and blocking nonspecific binding, sections were reacted with mouse anti-human sEH (A-5) monoclonal antibody (1 : 10 dilution; catalog no. SC-166961; Santa Cruz Biotechnology, Inc. Dallas, TX, USA), anti-human CK7 monoclonal antibody (1 : 1000 dilution, clone OV-TL 12/30, Dako Omnis, Agilent, Santa Clara, CA, USA), or anti-human CD68 monoclonal antibody (1 : 1000 dilution, clone KP1, Dako Omnis) at 4°C overnight. .. Further processing for colorimetric detection was according to the instructions for the VECTASTAIN Elite ABC Kit (Vector Laboratories, Burlingame, CA, USA) using diaminobenzidine as the peroxidase substrate.

    Binding Assay:

    Article Title: Increased Soluble Epoxide Hydrolase in Human Gestational Tissues from Pregnancies Complicated by Acute Chorioamnionitis
    Article Snippet: Immunohistochemistry Immunohistochemistry for sEH, cytokeratin 7 (CK7), and CD68 was performed as previously described [ ]. .. Briefly, after quenching endogenous peroxidase activity and blocking nonspecific binding, sections were reacted with mouse anti-human sEH (A-5) monoclonal antibody (1 : 10 dilution; catalog no. SC-166961; Santa Cruz Biotechnology, Inc. Dallas, TX, USA), anti-human CK7 monoclonal antibody (1 : 1000 dilution, clone OV-TL 12/30, Dako Omnis, Agilent, Santa Clara, CA, USA), or anti-human CD68 monoclonal antibody (1 : 1000 dilution, clone KP1, Dako Omnis) at 4°C overnight. .. Further processing for colorimetric detection was according to the instructions for the VECTASTAIN Elite ABC Kit (Vector Laboratories, Burlingame, CA, USA) using diaminobenzidine as the peroxidase substrate.

    Immunohistochemistry:

    Article Title: Expression of LIGHT/TNFSF14 and Its Receptors, HVEM and LTβR, Correlates with the Severity of Fibrosis in Lacrimal Sacs from Patients with Lacrimal Duct Obstruction
    Article Snippet: Fibrosis was evidenced using Masson’s trichrome staining in Ventana Benchmark and assessed semiquantitatively according to the following scoring system: 0 = no fibrosis (lack of collagen deposits), 1 = mild fibrosis (focal fibrotic expansion network of collagen, mainly in the perivascular areas), 2 = moderate fibrosis (focal dense increase of collagen bands) and 3 = severe fibrosis (diffuse, strong, dense collagen band expansion in the stroma). .. Identification of subsets of mononuclear cells was done by immunohistochemistry using specific antibodies: anti-CD68 (monoclonal mouse anti-human CD68/FITC clone KP1, Dako-Agilent) for macrophages, anti-CD3 antibody (mouse monoclonal anti-CD3 concentrate clone F7.2.38 Dako Omnis, Agilent) for T cells and anti-CD20 antibodies (monoclonal mouse clone L26, Dako Omnis, Agilent) for B cells. .. The staining was done on an Omnis autostainer using protocols, preceded by antigen retrieval in PTLink (pH = 9.0).

    Article Title: A Case of Adenocarcinoma of Uterus Masculinus in a Pomeranian Dog
    Article Snippet: .. On immunohistochemistry, neoplastic cells were positive for cytokeratin-7 (Dako Omnis, clone OV-TL 12/30; ) and vimentin (Dako Omnis, clone V9; ); moreover, smooth muscle cells and occasional neoplastic cells showed nuclear immunoreactivity for estrogen receptor α (Dako Omnis, clone EP1; ). ..

    other:

    Article Title: Automated sequential chromogenic IHC double staining with two HRP substrates
    Article Snippet: Dako Omnis protocol. (DOCX) (22K, docx)

    Staining:

    Article Title: Phase II Prospective Randomized Trial of Weight Loss Prior to Radical Prostatectomy (PCAN-17-0128)
    Article Snippet: Immunohistochemistry Serial sections for immunohistochemical analyses were cut from archived paraffin embedded blocks with the largest cancer volume and with the Gleason grade corresponding to the grade on the final pathology report. .. Slides were stained for Ki67 (monoclonal mouse anti-human Ki67 antigen [Dako Omnis/Agilent, Santa Clara, CA]) and TUNEL (ApopTag® Plus Peroxidase In Situ Apoptosis Kit [Millipore, Temecula, CA]) ( ) to assess proliferation and apoptosis, respectively. ..

    TUNEL Assay:

    Article Title: Phase II Prospective Randomized Trial of Weight Loss Prior to Radical Prostatectomy (PCAN-17-0128)
    Article Snippet: Immunohistochemistry Serial sections for immunohistochemical analyses were cut from archived paraffin embedded blocks with the largest cancer volume and with the Gleason grade corresponding to the grade on the final pathology report. .. Slides were stained for Ki67 (monoclonal mouse anti-human Ki67 antigen [Dako Omnis/Agilent, Santa Clara, CA]) and TUNEL (ApopTag® Plus Peroxidase In Situ Apoptosis Kit [Millipore, Temecula, CA]) ( ) to assess proliferation and apoptosis, respectively. ..

    In Situ:

    Article Title: Phase II Prospective Randomized Trial of Weight Loss Prior to Radical Prostatectomy (PCAN-17-0128)
    Article Snippet: Immunohistochemistry Serial sections for immunohistochemical analyses were cut from archived paraffin embedded blocks with the largest cancer volume and with the Gleason grade corresponding to the grade on the final pathology report. .. Slides were stained for Ki67 (monoclonal mouse anti-human Ki67 antigen [Dako Omnis/Agilent, Santa Clara, CA]) and TUNEL (ApopTag® Plus Peroxidase In Situ Apoptosis Kit [Millipore, Temecula, CA]) ( ) to assess proliferation and apoptosis, respectively. ..

    Incubation:

    Article Title: The Pro-Oxidant, Apoptotic and Anti-Angiogenic Effects of Selenium Supplementation on Colorectal Tumors Induced by 1,2-Dimethylhydrazine in BALB/C Mice
    Article Snippet: Immunohistochemical analysis of CDX-2, VEGF and caspase 3For immunohistochemical examination, paraffinembedded sections were first dewaxed, followed by an antigen-retrieval process and a series of blocking steps. .. After that, the sections were incubated separately overnight with the following primary antibodies: CDX-2 monoclonal antibody (M3636, Dako Omnis, UK), VEGF polyclonal antibody (RB-222-R7, Thermo Fisher Scientific, USA), caspase-3 polyclonal antibody (PA1-29157, Thermo Fisher Scientific, USA). .. The slides were washed thoroughly and then incubated with secondary antibody: (HRP Envision kit, DAKO, Agilent, USA).

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  • 97
    Agilent technologies real peroxidase blocking solution
    Real Peroxidase Blocking Solution, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real peroxidase blocking solution/product/Agilent technologies
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    real peroxidase blocking solution - by Bioz Stars, 2021-04
    97/100 stars
      Buy from Supplier

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