reagents damgo  (Millipore)


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  • 99
    Name:
    Methods and Reagents
    Description:
    This book aims to stimulate and promote the wide ranging aspects of green chemistry and its major role in ensuring sustainable development It covers the following areas green chemistry green reagents and atom economy safeguarding the atmosphere industrial green catalysis alternative reaction conditions biocatalysis and green chemistry This book is based on the third edition of the Collection of Lectures of the Summer Schools on Green Chemistry held in Venice Italy in the summers of 1998 2003
    Catalog Number:
    z731145
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    Structured Review

    Millipore reagents damgo
    Methods and Reagents
    This book aims to stimulate and promote the wide ranging aspects of green chemistry and its major role in ensuring sustainable development It covers the following areas green chemistry green reagents and atom economy safeguarding the atmosphere industrial green catalysis alternative reaction conditions biocatalysis and green chemistry This book is based on the third edition of the Collection of Lectures of the Summer Schools on Green Chemistry held in Venice Italy in the summers of 1998 2003
    https://www.bioz.com/result/reagents damgo/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    reagents damgo - by Bioz Stars, 2020-09
    99/100 stars

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    Transfection:

    Article Title: The cyclin-like protein Spy1/RINGO promotes mammary transformation and is elevated in human breast cancer
    Article Snippet: .. Transfection/infection methods Cells were transfected using polyethylenimine (PEI) branched reagent (408727; Sigma). .. In brief, for experiments using a fixed amount of DNA per construct 10 μg of DNA was mixed with 50 μL of 150 mM NaCl and 3 μL of 10 mg/ml PEI for 10 min then added to a 10 cm tissue culture plate.

    Article Title: Melittin Derived Peptides for Nanoparticle Based siRNA Transfection
    Article Snippet: .. B16-F10 cells were cultured on glass coverslips and transfected with Cy3-labeled double stranded 21 base pair oligonucleotides (Sigma) according to standard transfection procedures. .. 12 hours post transfection, cells were washed 3x in PBS and fixed in 4% PFA before mounting on glass slides (Vectashield Mounting Medium with DAPI, Vector Labs, Burlingame, CA).

    other:

    Article Title: Piperlongumine restores the balance of autophagy and apoptosis by increasing BCL2 phosphorylation in rotenone-induced Parkinson disease models
    Article Snippet: Reagents Antibodies against the following proteins were used in the study: ACTB (1:5000; Sigma-Aldrich, A5060), BAX (1:1000; Cell Signaling Technology, 2772), BCL2 (1:1000; Cell Signaling Technology, 3498), phosphorylated (p-)BCL2 (Ser70) (1:1000; Cell Signaling Technology, 2827), BECN1 (1:1000; Cell Signaling Technology, 3495), IgG (1:1000; Sigma-Aldrich, I5006), p-MAPK8/JNK1 (1:1000; Cell Signaling Technology, 4668 [we note that this antibody may react with phosphorylated MAPK1/3 and MAPK/p38]), MAPK8/JNK1 (1:1000; Cell Signaling Technology, 3708 [we note that this antibody may cross-react with MAPK9/JNK2]), LC3B (1:1,000; Novus Biologicals, NB100-2220), NFE2L2/NRF2 (1:1000; Cell Signaling Technology, 12721), SQSTM1 (1:1000; Cell Signaling Technology, 5114), TH (1:1000; Sigma-Aldrich, T2928), and WIPI2 (1:1000; Cell Signaling Technology, 8567).

    Article Title: Loss of DLG5 promotes breast cancer malignancy by inhibiting the Hippo signaling pathway
    Article Snippet: Antibodies and Reagents Antibodies used in this study included anti-DLG5 (Sigma), anti-N-cadherin (BD transduction), anti-E-cadherin (BD Biosciences), anti-vimentin (Sigma), anti-ZO1 (Sigma) and Hippo Signaling Antibody Sampler Kit (CST).

    Article Title: Integrative Genomics Identifies YY1AP1 as an Oncogenic Driver in EpCAM+ AFP+ Hepatocellular Carcinoma
    Article Snippet: Reagents Antibodies to YY1AP1 (HPA006986) from Sigma; antibody to EpCAM (MAB960) from R & D Systems; anti-DDK monoclonal antibody (TA50011-100) from Origen; antibody to p53 from Santa Cruz Biotechnology (sc-126); antibodies to TCF4 (#2953), E-Cadherin (#5296), AFP (#2137), cleaved caspase 3 (Asp175, #9961), c-Myc (#5605), Sox2 (#3579), β-catenin (#8480), Histone H2B (#12364), ub-Histone H2B (#5546), Methyl-Histone H3 (#9847) and Acetyl-Histone H3 (#9933) antibody sample kits, Tri-Mehyl-Histone H3 (Lys4) (#9751) and Tri-Methyl-Histone H3 (Lys27) (#9733) were from Cell Signaling Technology.

    Cell Culture:

    Article Title: Melittin Derived Peptides for Nanoparticle Based siRNA Transfection
    Article Snippet: .. B16-F10 cells were cultured on glass coverslips and transfected with Cy3-labeled double stranded 21 base pair oligonucleotides (Sigma) according to standard transfection procedures. .. 12 hours post transfection, cells were washed 3x in PBS and fixed in 4% PFA before mounting on glass slides (Vectashield Mounting Medium with DAPI, Vector Labs, Burlingame, CA).

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  • 99
    Millipore u0126
    Effects of inhibitors related to the GDNF production cascade on the amitriptyline-evoked increase of Δ Z in C6 cells. A, effects of GM6001 ( GM ), SU5420 ( SU ), PD173074 ( PD ), and <t>U0126</t> on the increase of Δ Z evoked by amitriptyline in C6 cells.
    U0126, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/u0126/product/Millipore
    Average 99 stars, based on 91 article reviews
    Price from $9.99 to $1999.99
    u0126 - by Bioz Stars, 2020-09
    99/100 stars
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    92
    Millipore nf449
    Effects of α-subunit of G protein inhibitors on amitriptyline-evoked GDNF production. A , effects of pertussis toxin ( PTX ), <t>NF449</t> ( NF ), and YM-254890 ( YM ) on the amitriptyline-evoked GDNF mRNA expression. C6 cells were pretreated with 100 ng/ml
    Nf449, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nf449/product/Millipore
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nf449 - by Bioz Stars, 2020-09
    92/100 stars
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    94
    Millipore cfa
    Effects of α-subunit of G protein inhibitors on amitriptyline-evoked GDNF production. A , effects of pertussis toxin ( PTX ), <t>NF449</t> ( NF ), and YM-254890 ( YM ) on the amitriptyline-evoked GDNF mRNA expression. C6 cells were pretreated with 100 ng/ml
    Cfa, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 617 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cfa/product/Millipore
    Average 94 stars, based on 617 article reviews
    Price from $9.99 to $1999.99
    cfa - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Effects of inhibitors related to the GDNF production cascade on the amitriptyline-evoked increase of Δ Z in C6 cells. A, effects of GM6001 ( GM ), SU5420 ( SU ), PD173074 ( PD ), and U0126 on the increase of Δ Z evoked by amitriptyline in C6 cells.

    Journal: The Journal of Biological Chemistry

    Article Title: Tricyclic Antidepressant Amitriptyline-induced Glial Cell Line-derived Neurotrophic Factor Production Involves Pertussis Toxin-sensitive Gαi/o Activation in Astroglial Cells *

    doi: 10.1074/jbc.M114.622415

    Figure Lengend Snippet: Effects of inhibitors related to the GDNF production cascade on the amitriptyline-evoked increase of Δ Z in C6 cells. A, effects of GM6001 ( GM ), SU5420 ( SU ), PD173074 ( PD ), and U0126 on the increase of Δ Z evoked by amitriptyline in C6 cells.

    Article Snippet: Reagents were obtained from the following sources: amitriptyline (Wako Pure Chemical Industries, Ltd., Osaka, Japan); serotonin creatinine sulfate monohydrate, nor-binaltorphimine dihydrochloride (nor-BNI), and [ d -Ala2 , N -MePhe4 ,Gly-ol]enkephalin (DAMGO) (Sigma); FGF2 human recombinant (Roche Diagnostics); pertussis toxin, NF449, and U0126 (Calbiochem); GM6001, SU5402, and PD173074 (Merck KGaA, Darmstadt, Germany); acetylcholine and isoproterenol (Nacalai Tesque, Kyoto, Japan).

    Techniques:

    Effects of α-subunit of G protein inhibitors on amitriptyline-evoked GDNF production. A , effects of pertussis toxin ( PTX ), NF449 ( NF ), and YM-254890 ( YM ) on the amitriptyline-evoked GDNF mRNA expression. C6 cells were pretreated with 100 ng/ml

    Journal: The Journal of Biological Chemistry

    Article Title: Tricyclic Antidepressant Amitriptyline-induced Glial Cell Line-derived Neurotrophic Factor Production Involves Pertussis Toxin-sensitive Gαi/o Activation in Astroglial Cells *

    doi: 10.1074/jbc.M114.622415

    Figure Lengend Snippet: Effects of α-subunit of G protein inhibitors on amitriptyline-evoked GDNF production. A , effects of pertussis toxin ( PTX ), NF449 ( NF ), and YM-254890 ( YM ) on the amitriptyline-evoked GDNF mRNA expression. C6 cells were pretreated with 100 ng/ml

    Article Snippet: Reagents were obtained from the following sources: amitriptyline (Wako Pure Chemical Industries, Ltd., Osaka, Japan); serotonin creatinine sulfate monohydrate, nor-binaltorphimine dihydrochloride (nor-BNI), and [ d -Ala2 , N -MePhe4 ,Gly-ol]enkephalin (DAMGO) (Sigma); FGF2 human recombinant (Roche Diagnostics); pertussis toxin, NF449, and U0126 (Calbiochem); GM6001, SU5402, and PD173074 (Merck KGaA, Darmstadt, Germany); acetylcholine and isoproterenol (Nacalai Tesque, Kyoto, Japan).

    Techniques: Expressing

    Effects of G protein inhibitors on the increase in Δ Z evoked by amitriptyline in C6 cells and rat astrocytes or μ-opioid receptor agonist DAMGO in HEK293 cells expressing μ-opioid receptors. A, effects of PTX, NF449 ( NF ), and YM-254890

    Journal: The Journal of Biological Chemistry

    Article Title: Tricyclic Antidepressant Amitriptyline-induced Glial Cell Line-derived Neurotrophic Factor Production Involves Pertussis Toxin-sensitive Gαi/o Activation in Astroglial Cells *

    doi: 10.1074/jbc.M114.622415

    Figure Lengend Snippet: Effects of G protein inhibitors on the increase in Δ Z evoked by amitriptyline in C6 cells and rat astrocytes or μ-opioid receptor agonist DAMGO in HEK293 cells expressing μ-opioid receptors. A, effects of PTX, NF449 ( NF ), and YM-254890

    Article Snippet: Reagents were obtained from the following sources: amitriptyline (Wako Pure Chemical Industries, Ltd., Osaka, Japan); serotonin creatinine sulfate monohydrate, nor-binaltorphimine dihydrochloride (nor-BNI), and [ d -Ala2 , N -MePhe4 ,Gly-ol]enkephalin (DAMGO) (Sigma); FGF2 human recombinant (Roche Diagnostics); pertussis toxin, NF449, and U0126 (Calbiochem); GM6001, SU5402, and PD173074 (Merck KGaA, Darmstadt, Germany); acetylcholine and isoproterenol (Nacalai Tesque, Kyoto, Japan).

    Techniques: Expressing