reaction buffer for phi29 dna polymerase 10x  (Thermo Fisher)


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    Name:
    Reaction Buffer for phi29 DNA Polymerase 10X
    Description:
    Thermo Scientific 10X Buffer for phi29 DNA Polymerase is the optimal buffer recommended for use with highly processive phi 29 DNA polymerase
    Catalog Number:
    b62
    Price:
    None
    Applications:
    Cloning|DNA & RNA Purification & Analysis|Gene Synthesis, Seamless Cloning, & Assembly|Seamless Cloning & Genetic Assembly
    Category:
    Lab Reagents and Chemicals
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    Structured Review

    Thermo Fisher reaction buffer for phi29 dna polymerase 10x
    Thermo Scientific 10X Buffer for phi29 DNA Polymerase is the optimal buffer recommended for use with highly processive phi 29 DNA polymerase
    https://www.bioz.com/result/reaction buffer for phi29 dna polymerase 10x/product/Thermo Fisher
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    reaction buffer for phi29 dna polymerase 10x - by Bioz Stars, 2020-11
    98/100 stars

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    Related Articles

    Amplification:

    Article Title: Ictal onset sites and GABAergic neuron loss in epileptic pilocarpine-treated rats
    Article Snippet: .. Amplification solution contained 1000 units/ml NxGen phi29 DNA polymerase in phi29 polymerase buffer (Lucigen) and 1 mM dNTP mix (0.25 mM/nucleotide, Invitrogen), 0.4 units/μl RNaseOUT, 5% glycerol, and 0.4 mg/ml BSA; sections incubated overnight at 37°C. .. The next day sections were rinsed in a label probe hybridization buffer of 2x saline-sodium citrate (Invitrogen) and 20% deionized formamide (Invitrogen) in DEPC-treated water.

    Article Title: Ictal onset sites and GABAergic neuron loss in epileptic pilocarpine-treated rats
    Article Snippet: .. Sections were rinsed in hybridization buffer followed by phi29 polymerase buffer before the rolling circle amplification reaction. .. Amplification solution contained 1000 units/ml NxGen phi29 DNA polymerase in phi29 polymerase buffer (Lucigen) and 1 mM dNTP mix (0.25 mM/nucleotide, Invitrogen), 0.4 units/μl RNaseOUT, 5% glycerol, and 0.4 mg/ml BSA; sections incubated overnight at 37°C.

    Cell Culture:

    Article Title: Immune-privileged embryonic Swiss mouse STO and STO cell-derived progenitor cells: major histocompatibility complex and cell differentiation antigen expression patterns resemble those of human embryonic stem cell lines
    Article Snippet: .. STO and SCD lines 3(8)21 and 3(8)21-EGFP were cultured by standard procedures; , passage numbers were recorded and cell counts performed as described elsewhere., Swiss mouse q-haplotype NIH3T3 cells, d-haplotype BALB/c 3T3 cells, human embryonic kidney 293 cells (American Type Culture Collection #CRL-1573), mouse H-2Kb/d -, H-2Db/d - and H-2Lb/d -positive B6-2 cells and primary BALB/c kidney cells prepared from trypsinized tissue were cultured as described previously., Fetal bovine serum (FBS; Invitrogen, Carlsbad, CA) was heat-inactivated at 56° for 30 min. .. Unless noted, cells were cultured in 3- or 10-cm-diameter plastic tissue culture dishes (BD Falcon™, Bedford, MA).

    TaqMan microRNA Assay:

    Article Title: Human hepatocellular carcinoma cell-specific miRNAs reveal the differential expression of miR-24 and miR-27a in cirrhotic/non-cirrhotic HCC
    Article Snippet: .. The reverse transcriptase reaction was performed by incubating the samples at 16°C for 30 min, 42°C for 30 min, and 85°C for 5 min. Each PCR reaction (20 μ l) contained 1.3 μ l of reverse transcriptase product, 10 μ l of Taq-Man 2X Universal PCR Master Mix, and 1 μ l of the appropriate TaqMan MicroRNA Assay solution containing primers and probes for each miR of interest. .. The PCR mixtures were incubated at 95°C for 10 min followed by 40 cycles of 95°C for 15 sec and 60°C for 60 sec. PCRs were performed in triplicate using a 7500 Real-Time PCR system.

    Concentration Assay:

    Article Title: Recording the age of RNA with deamination
    Article Snippet: .. Cell concentration was counted and the appropriate volume of cells was added to a 10x reaction for a targeted cell recovery of 2000 cells. .. The reaction was run through a 10x chip according to the 10x Genomics Chromium Single Cell protocol.

    Incubation:

    Article Title: Ictal onset sites and GABAergic neuron loss in epileptic pilocarpine-treated rats
    Article Snippet: .. Amplification solution contained 1000 units/ml NxGen phi29 DNA polymerase in phi29 polymerase buffer (Lucigen) and 1 mM dNTP mix (0.25 mM/nucleotide, Invitrogen), 0.4 units/μl RNaseOUT, 5% glycerol, and 0.4 mg/ml BSA; sections incubated overnight at 37°C. .. The next day sections were rinsed in a label probe hybridization buffer of 2x saline-sodium citrate (Invitrogen) and 20% deionized formamide (Invitrogen) in DEPC-treated water.

    other:

    Article Title: microRNA-944 overexpression is a biomarker for poor prognosis of advanced cervical cancer
    Article Snippet: The reverse transcriptase reaction was carried out at 25 °C for 10 min, at 37 °C for 50 min, and at 70 °C for 15 min.

    Article Title: MicroRNA-103-1 Selectively Downregulates Brain NCX1 and Its Inhibition by Anti-miRNA Ameliorates Stroke Damage and Neurological Deficits
    Article Snippet: The semiquantitative polymerase reaction was performed as previously described.

    Polymerase Chain Reaction:

    Article Title: Human hepatocellular carcinoma cell-specific miRNAs reveal the differential expression of miR-24 and miR-27a in cirrhotic/non-cirrhotic HCC
    Article Snippet: .. The reverse transcriptase reaction was performed by incubating the samples at 16°C for 30 min, 42°C for 30 min, and 85°C for 5 min. Each PCR reaction (20 μ l) contained 1.3 μ l of reverse transcriptase product, 10 μ l of Taq-Man 2X Universal PCR Master Mix, and 1 μ l of the appropriate TaqMan MicroRNA Assay solution containing primers and probes for each miR of interest. .. The PCR mixtures were incubated at 95°C for 10 min followed by 40 cycles of 95°C for 15 sec and 60°C for 60 sec. PCRs were performed in triplicate using a 7500 Real-Time PCR system.

    Hybridization:

    Article Title: Ictal onset sites and GABAergic neuron loss in epileptic pilocarpine-treated rats
    Article Snippet: .. Sections were rinsed in hybridization buffer followed by phi29 polymerase buffer before the rolling circle amplification reaction. .. Amplification solution contained 1000 units/ml NxGen phi29 DNA polymerase in phi29 polymerase buffer (Lucigen) and 1 mM dNTP mix (0.25 mM/nucleotide, Invitrogen), 0.4 units/μl RNaseOUT, 5% glycerol, and 0.4 mg/ml BSA; sections incubated overnight at 37°C.

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  • 98
    Thermo Fisher transcriptase reaction
    Transcriptase Reaction, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transcriptase reaction/product/Thermo Fisher
    Average 98 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    transcriptase reaction - by Bioz Stars, 2020-11
    98/100 stars
      Buy from Supplier

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