Structured Review

Thermo Fisher ratp
Ratp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ratp/product/Thermo Fisher
Average 91 stars, based on 11 article reviews
Price from $9.99 to $1999.99
ratp - by Bioz Stars, 2020-08
91/100 stars

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Related Articles

Polymerase Chain Reaction:

Article Title: A high-throughput, quantitative cell-based screen for efficient tailoring of RNA device activity
Article Snippet: .. A total of 1–2 µg of PCR product was transcribed in a 25 µl reaction, consisting of the following components: 1×RNA Pol Reaction Buffer (New England Biolabs), 2.5 mM rATP, 2.5 mM rCTP, 2.5 mM rUTP, 0.25 mM rGTP, 1 µl RNaseOUT (Invitrogen), 10 mM MgCl2 , 1 µl T7 Polymerase (New England Biolabs) and 0.5 µCi α-32 P-GTP (MP Biomedicals, Solon, OH). ..

Article Title: Repair of the tRNA-Like CCA Sequence in a Multipartite Positive-Strand RNA Virus
Article Snippet: .. These RNAs were subjected to polyadenylation with poly(A) polymerase and 5 mM rATP for 30 min at 37°C, and then they were subjected to cDNA synthesis using Moloney murine leukemia virus reverse transcriptase (Invitrogen, Inc.) with an oligo(dT)18 primer at 37°C for 60 min. PCR amplification of the cDNA used the oligo(dT)18 primer and a BMV RNA-specific sense primer. .. We found that reverse transcription-PCR (RT-PCR) fragments derived from BMV RNAs that had wild-type CCA ends could be sequenced directly.

Incubation:

Article Title: A Land Plant-Specific Transcription Factor Directly Enhances Transcription of a Pathogenic Noncoding RNA Template by DNA-Dependent RNA Polymerase II [OPEN]
Article Snippet: .. Transcription reactions (20 μL) containing 50 mM HEPES-KOH, pH 7.9, 1 mM MnCl2 , 6 mM MgCl2 , 40 mM (NH4 )2 SO4 , 10% (v/v) glycerol, 20 unit of RNase inhibitor, 500 μM rATP, 500 μM rGTP, 500 μM rCTP, 500 μM rUTP, 10 ng/μL PSTVd, 4 μM protein, and 9 μL FLAG-Pol II were performed at 28 ° C for 4 h. The reactions were terminated by proteinase K (Invitrogen) treatment for 15 min at 37°C, followed by incubation at 95°C for 5 min. .. The transcription products were separated on 5% (w/v) polyacrylamide/8 M urea gel, transferred to Hybond-XL nylon membranes (Amersham Biosciences) by Trans-Blot SD semidry electrophoretic transfer cell (Bio-Rad), and immobilized by UV cross-linking.

Article Title: Regional Susceptibility to Domoic Acid in Primary Astrocyte Cells Cultured from the Brain Stem and Hippocampus
Article Snippet: .. The ds-cDNA was incubated with each of 3 μL rATP (75mM), rGTP (75 mM), rUTP (75 mM), rCTP (15 mM), 10x transcription buffer, T7 enzyme mix, and 4.5 μL biotin-14-CTP (10 mM) (Invitrogen Canada Inc., Burlington. .. The synthesized cRNA probes were purified using Micro-Spin Columns.

Amplification:

Article Title: Repair of the tRNA-Like CCA Sequence in a Multipartite Positive-Strand RNA Virus
Article Snippet: .. These RNAs were subjected to polyadenylation with poly(A) polymerase and 5 mM rATP for 30 min at 37°C, and then they were subjected to cDNA synthesis using Moloney murine leukemia virus reverse transcriptase (Invitrogen, Inc.) with an oligo(dT)18 primer at 37°C for 60 min. PCR amplification of the cDNA used the oligo(dT)18 primer and a BMV RNA-specific sense primer. .. We found that reverse transcription-PCR (RT-PCR) fragments derived from BMV RNAs that had wild-type CCA ends could be sequenced directly.

Electrophoretic Mobility Shift Assay:

Article Title: Rotavirus Genomic RNA Complex Forms via Specific RNA–RNA Interactions: Disruption of RNA Complex Inhibits Virus Infectivity
Article Snippet: .. Electrophoretic Mobility Shift Assay (EMSA) for RNA–RNA Interaction RNA segments were co-transcribed using 150 ng of linearized plasmid (S8–S11) in a buffer containing 40 mM Tris-HCl (pH 7.5), 10 mM MgCl2 , 20 mM NaCl2 , 3 mM spermidine, 50 mM dithiothreitol (DTT), 5 mM each of rATP, rUTP, rCTP, and rGTP, 10 U of RNase inhibitor and 40 U of T7 RNA polymerase (Thermo Scientific, Waltham, MA, USA). .. The mixture was incubated for 3 h at 37 °C, followed by RNase-free DNase One treatment.

Plasmid Preparation:

Article Title: Rotavirus Genomic RNA Complex Forms via Specific RNA–RNA Interactions: Disruption of RNA Complex Inhibits Virus Infectivity
Article Snippet: .. Electrophoretic Mobility Shift Assay (EMSA) for RNA–RNA Interaction RNA segments were co-transcribed using 150 ng of linearized plasmid (S8–S11) in a buffer containing 40 mM Tris-HCl (pH 7.5), 10 mM MgCl2 , 20 mM NaCl2 , 3 mM spermidine, 50 mM dithiothreitol (DTT), 5 mM each of rATP, rUTP, rCTP, and rGTP, 10 U of RNase inhibitor and 40 U of T7 RNA polymerase (Thermo Scientific, Waltham, MA, USA). .. The mixture was incubated for 3 h at 37 °C, followed by RNase-free DNase One treatment.

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    Thermo Fisher ratp
    Ratp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ratp/product/Thermo Fisher
    Average 91 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    ratp - by Bioz Stars, 2020-08
    91/100 stars
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