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Thermo Fisher ratp
Ratp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ratp/product/Thermo Fisher
Average 93 stars, based on 10 article reviews
Price from $9.99 to $1999.99
ratp - by Bioz Stars, 2020-01
93/100 stars

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Real-time Polymerase Chain Reaction:

Article Title: Promotion of Hendra Virus Replication by MicroRNA 146a
Article Snippet: .. For miRNA-specific quantitative real-time PCR (qRT-PCR), sample polyadenylation was performed prior to cDNA synthesis by incubating RNA (∼200 ng) with 1 U poly(A) polymerase (Affymetrix, Santa Clara, CA), 0.5 nM rATP (Ambion, Carlsbad, CA) in 1× poly(A) polymerase buffer (In Vitro Technologies) at 37°C for 30 min and then at 95°C for 5 min. cDNA synthesis was performed using Superscript III reverse transcriptase (Invitrogen, Carlsbad, CA) according to the manufacturer's guidelines. .. For miRNA cDNA synthesis, a modified oligo(dT) primer (miR-PTA) was used as described previously ( ). qRT-PCR was performed using Sybr green (Applied Biosystems, Foster City, CA) on an ABI Prism 7700 sequence detection system (Applied Biosystems).

In Vivo:

Article Title: Promotion of Hendra Virus Replication by MicroRNA 146a
Article Snippet: For miRNA-specific quantitative real-time PCR (qRT-PCR), sample polyadenylation was performed prior to cDNA synthesis by incubating RNA (∼200 ng) with 1 U poly(A) polymerase (Affymetrix, Santa Clara, CA), 0.5 nM rATP (Ambion, Carlsbad, CA) in 1× poly(A) polymerase buffer (In Vitro Technologies) at 37°C for 30 min and then at 95°C for 5 min. cDNA synthesis was performed using Superscript III reverse transcriptase (Invitrogen, Carlsbad, CA) according to the manufacturer's guidelines. .. For gene expression and in vivo miRNA expression, data were analyzed using the ΔΔ CT method and were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) for gene detection and 5S RNA for miRNAs.

In Vitro:

Article Title: Promotion of Hendra Virus Replication by MicroRNA 146a
Article Snippet: .. For miRNA-specific quantitative real-time PCR (qRT-PCR), sample polyadenylation was performed prior to cDNA synthesis by incubating RNA (∼200 ng) with 1 U poly(A) polymerase (Affymetrix, Santa Clara, CA), 0.5 nM rATP (Ambion, Carlsbad, CA) in 1× poly(A) polymerase buffer (In Vitro Technologies) at 37°C for 30 min and then at 95°C for 5 min. cDNA synthesis was performed using Superscript III reverse transcriptase (Invitrogen, Carlsbad, CA) according to the manufacturer's guidelines. .. For miRNA cDNA synthesis, a modified oligo(dT) primer (miR-PTA) was used as described previously ( ). qRT-PCR was performed using Sybr green (Applied Biosystems, Foster City, CA) on an ABI Prism 7700 sequence detection system (Applied Biosystems).

Modification:

Article Title: Promotion of Hendra Virus Replication by MicroRNA 146a
Article Snippet: For miRNA-specific quantitative real-time PCR (qRT-PCR), sample polyadenylation was performed prior to cDNA synthesis by incubating RNA (∼200 ng) with 1 U poly(A) polymerase (Affymetrix, Santa Clara, CA), 0.5 nM rATP (Ambion, Carlsbad, CA) in 1× poly(A) polymerase buffer (In Vitro Technologies) at 37°C for 30 min and then at 95°C for 5 min. cDNA synthesis was performed using Superscript III reverse transcriptase (Invitrogen, Carlsbad, CA) according to the manufacturer's guidelines. .. For miRNA cDNA synthesis, a modified oligo(dT) primer (miR-PTA) was used as described previously ( ). qRT-PCR was performed using Sybr green (Applied Biosystems, Foster City, CA) on an ABI Prism 7700 sequence detection system (Applied Biosystems).

Infection:

Article Title: Promotion of Hendra Virus Replication by MicroRNA 146a
Article Snippet: Male ferrets (aged 12 to 18 months) and three adult mares were infected with Hendra virus as part of studies described previously ( , ). .. For miRNA-specific quantitative real-time PCR (qRT-PCR), sample polyadenylation was performed prior to cDNA synthesis by incubating RNA (∼200 ng) with 1 U poly(A) polymerase (Affymetrix, Santa Clara, CA), 0.5 nM rATP (Ambion, Carlsbad, CA) in 1× poly(A) polymerase buffer (In Vitro Technologies) at 37°C for 30 min and then at 95°C for 5 min. cDNA synthesis was performed using Superscript III reverse transcriptase (Invitrogen, Carlsbad, CA) according to the manufacturer's guidelines.

Purification:

Article Title: Promotion of Hendra Virus Replication by MicroRNA 146a
Article Snippet: Paragraph title: Purification of RNA from Hendra virus animal trials, reverse transcription, and quantitative real-time PCR. ... For miRNA-specific quantitative real-time PCR (qRT-PCR), sample polyadenylation was performed prior to cDNA synthesis by incubating RNA (∼200 ng) with 1 U poly(A) polymerase (Affymetrix, Santa Clara, CA), 0.5 nM rATP (Ambion, Carlsbad, CA) in 1× poly(A) polymerase buffer (In Vitro Technologies) at 37°C for 30 min and then at 95°C for 5 min. cDNA synthesis was performed using Superscript III reverse transcriptase (Invitrogen, Carlsbad, CA) according to the manufacturer's guidelines.

SYBR Green Assay:

Article Title: Promotion of Hendra Virus Replication by MicroRNA 146a
Article Snippet: For miRNA-specific quantitative real-time PCR (qRT-PCR), sample polyadenylation was performed prior to cDNA synthesis by incubating RNA (∼200 ng) with 1 U poly(A) polymerase (Affymetrix, Santa Clara, CA), 0.5 nM rATP (Ambion, Carlsbad, CA) in 1× poly(A) polymerase buffer (In Vitro Technologies) at 37°C for 30 min and then at 95°C for 5 min. cDNA synthesis was performed using Superscript III reverse transcriptase (Invitrogen, Carlsbad, CA) according to the manufacturer's guidelines. .. For miRNA cDNA synthesis, a modified oligo(dT) primer (miR-PTA) was used as described previously ( ). qRT-PCR was performed using Sybr green (Applied Biosystems, Foster City, CA) on an ABI Prism 7700 sequence detection system (Applied Biosystems).

Sequencing:

Article Title: Promotion of Hendra Virus Replication by MicroRNA 146a
Article Snippet: For miRNA-specific quantitative real-time PCR (qRT-PCR), sample polyadenylation was performed prior to cDNA synthesis by incubating RNA (∼200 ng) with 1 U poly(A) polymerase (Affymetrix, Santa Clara, CA), 0.5 nM rATP (Ambion, Carlsbad, CA) in 1× poly(A) polymerase buffer (In Vitro Technologies) at 37°C for 30 min and then at 95°C for 5 min. cDNA synthesis was performed using Superscript III reverse transcriptase (Invitrogen, Carlsbad, CA) according to the manufacturer's guidelines. .. For miRNA cDNA synthesis, a modified oligo(dT) primer (miR-PTA) was used as described previously ( ). qRT-PCR was performed using Sybr green (Applied Biosystems, Foster City, CA) on an ABI Prism 7700 sequence detection system (Applied Biosystems).

Quantitative RT-PCR:

Article Title: Promotion of Hendra Virus Replication by MicroRNA 146a
Article Snippet: .. For miRNA-specific quantitative real-time PCR (qRT-PCR), sample polyadenylation was performed prior to cDNA synthesis by incubating RNA (∼200 ng) with 1 U poly(A) polymerase (Affymetrix, Santa Clara, CA), 0.5 nM rATP (Ambion, Carlsbad, CA) in 1× poly(A) polymerase buffer (In Vitro Technologies) at 37°C for 30 min and then at 95°C for 5 min. cDNA synthesis was performed using Superscript III reverse transcriptase (Invitrogen, Carlsbad, CA) according to the manufacturer's guidelines. .. For miRNA cDNA synthesis, a modified oligo(dT) primer (miR-PTA) was used as described previously ( ). qRT-PCR was performed using Sybr green (Applied Biosystems, Foster City, CA) on an ABI Prism 7700 sequence detection system (Applied Biosystems).

Expressing:

Article Title: Promotion of Hendra Virus Replication by MicroRNA 146a
Article Snippet: For miRNA-specific quantitative real-time PCR (qRT-PCR), sample polyadenylation was performed prior to cDNA synthesis by incubating RNA (∼200 ng) with 1 U poly(A) polymerase (Affymetrix, Santa Clara, CA), 0.5 nM rATP (Ambion, Carlsbad, CA) in 1× poly(A) polymerase buffer (In Vitro Technologies) at 37°C for 30 min and then at 95°C for 5 min. cDNA synthesis was performed using Superscript III reverse transcriptase (Invitrogen, Carlsbad, CA) according to the manufacturer's guidelines. .. For gene expression and in vivo miRNA expression, data were analyzed using the ΔΔ CT method and were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) for gene detection and 5S RNA for miRNAs.

Polymerase Chain Reaction:

Article Title: Promotion of Hendra Virus Replication by MicroRNA 146a
Article Snippet: For miRNA-specific quantitative real-time PCR (qRT-PCR), sample polyadenylation was performed prior to cDNA synthesis by incubating RNA (∼200 ng) with 1 U poly(A) polymerase (Affymetrix, Santa Clara, CA), 0.5 nM rATP (Ambion, Carlsbad, CA) in 1× poly(A) polymerase buffer (In Vitro Technologies) at 37°C for 30 min and then at 95°C for 5 min. cDNA synthesis was performed using Superscript III reverse transcriptase (Invitrogen, Carlsbad, CA) according to the manufacturer's guidelines. .. PCR cycling for gene detection was at 95°C for 10 min, followed by 40 cycles of 95°C for 15 s and 60°C for 1 min. PCR cycling for miRNA detection was at 95°C for 10 min, followed by 40 cycles of 95°C for 15 s, 57°C for 30 s, and 72°C for 30 s. A melting curve analysis was performed to eliminate primer-dimer artifacts and to verify the specificity of the assay.

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    Thermo Fisher ratp
    Ratp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ratp/product/Thermo Fisher
    Average 93 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    ratp - by Bioz Stars, 2020-01
    93/100 stars
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