Journal: Journal of immunology (Baltimore, Md. : 1950)
Article Title: The proteoglycan biglycan enhances antigen-specific T cell activation via MyD88 and TRIF pathways and triggers autoimmune perimyocarditis
doi: 10.4049/jimmunol.1003478
Figure Lengend Snippet: WT and biglycan −/− mice (C57BL/6) were used in a myocardial infarction model. Two weeks after induction of infarction by a ligation of the left anterior descending artery, mouse heart sections were analyzed for biglycan expression (upper lane, 400×), size of post-myocardial infarction (MI) fibrosis (evaluated as percentage of ischemic damage within heart section from 5 sections per mouse; H/E, 200×), intensity of HR3+ cell infiltration (200×) and intensity of CD3+ cell infiltration (lower lane, 200×). Cells were counted in (total) 10 HPF from 5 sections per mouse. Data are presented as mean ± SEM from 3 experiments in duplicates. Statistical differences were evaluated by unpaired t-test. **, p<0,01.
Article Snippet: Rat anti-mouse ER-HR3 antibody was purchased from Acris Antibodies GmbH, Germany.
Techniques: Ligation, Expressing