random primer mix 6 nmol  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Name:
    Random Primer Mix 6 nmol
    Description:
    Random Primer Mix 6 nmol
    Catalog Number:
    s1330s
    Price:
    99
    Size:
    6 nmol
    Category:
    Probes and Primers
    Buy from Supplier


    Structured Review

    New England Biolabs random primer mix 6 nmol
    Random Primer Mix 6 nmol
    Random Primer Mix 6 nmol
    https://www.bioz.com/result/random primer mix 6 nmol/product/New England Biolabs
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    random primer mix 6 nmol - by Bioz Stars, 2020-09
    95/100 stars

    Images

    Related Articles

    Synthesized:

    Article Title: Xenopus: An alternative model system for identifying muco-active agents
    Article Snippet: .. Total RNA was extracted using a PureLink RNA mini kit (Invitrogen 12183018A), and the cDNA was synthesized using a random primer (NEB S1330S) and GoScript Reverse Transcriptase (Promega A50047). .. Quantitative RT-PCR was performed using the LightCycler 480 instrument (Roche LC480).

    Article Title: Xenopus: An alternative model system for identifying muco-active agents
    Article Snippet: .. Quantitative RT-PCR Total RNA was extracted using a PureLink RNA mini kit (Invitrogen 12183018A), and the cDNA was synthesized using a random primer (NEB S1330S) and GoScript Reverse Transcriptase (Promega A50047). .. Quantitative RT-PCR was performed using the LightCycler 480 instrument (Roche LC480).

    Protease Inhibitor:

    Article Title: Fatostatin blocks ER exit of SCAP but inhibits cell growth in a SCAP-independent manner
    Article Snippet: .. We obtained fatostatin (341329), compactin (mevastatin, 474705), and N -acetyl-leucinyl-leucinyl-norleucinal (ALLN, 208719) from Millipore; FBS (heat-inactivated) from Atlanta Biologicals; RNA-STAT 60 from Tel-Test, Inc.; mevalonolactone (M4667), puromycin dihydrochloride (P8833), oleic acid-albumin (O3008), crystal violet (C3886), cholesterol (C3045), 25-hydroxycholesterol (H1015), and lipoprotein-deficient serum (LPDS; S5394) from Sigma-Aldrich; site-1 protease inhibitor PF-429242 from Shanghai API Chemicals (947303-87-9); cell culture medium high glucose DMEM (10-013), DMEM/F12 (10-092), and penicillin-streptomycin (30-002) from Corning Cellgro; FuGENE 6 and RNase-free DNase I (10104159001) from Roche Applied Science; random primer mix (S1330), M-MuLV reverse transcriptase (M0253L), murine RNase inhibitor (M0314L), and oligo d(T)23VN (S1327S) from New England Biolabs; GoTaq real-time PCR mix (A6002) and CellTiter 96 NonRadioactive cell proliferation kit (G4000) from Promega. .. Stock solutions of fatostatin (20 mM) and PF-429242 (50 mM) were made by dissolving chemicals in DMSO.

    Cell Culture:

    Article Title: Fatostatin blocks ER exit of SCAP but inhibits cell growth in a SCAP-independent manner
    Article Snippet: .. We obtained fatostatin (341329), compactin (mevastatin, 474705), and N -acetyl-leucinyl-leucinyl-norleucinal (ALLN, 208719) from Millipore; FBS (heat-inactivated) from Atlanta Biologicals; RNA-STAT 60 from Tel-Test, Inc.; mevalonolactone (M4667), puromycin dihydrochloride (P8833), oleic acid-albumin (O3008), crystal violet (C3886), cholesterol (C3045), 25-hydroxycholesterol (H1015), and lipoprotein-deficient serum (LPDS; S5394) from Sigma-Aldrich; site-1 protease inhibitor PF-429242 from Shanghai API Chemicals (947303-87-9); cell culture medium high glucose DMEM (10-013), DMEM/F12 (10-092), and penicillin-streptomycin (30-002) from Corning Cellgro; FuGENE 6 and RNase-free DNase I (10104159001) from Roche Applied Science; random primer mix (S1330), M-MuLV reverse transcriptase (M0253L), murine RNase inhibitor (M0314L), and oligo d(T)23VN (S1327S) from New England Biolabs; GoTaq real-time PCR mix (A6002) and CellTiter 96 NonRadioactive cell proliferation kit (G4000) from Promega. .. Stock solutions of fatostatin (20 mM) and PF-429242 (50 mM) were made by dissolving chemicals in DMSO.

    Real-time Polymerase Chain Reaction:

    Article Title: Fatostatin blocks ER exit of SCAP but inhibits cell growth in a SCAP-independent manner
    Article Snippet: .. We obtained fatostatin (341329), compactin (mevastatin, 474705), and N -acetyl-leucinyl-leucinyl-norleucinal (ALLN, 208719) from Millipore; FBS (heat-inactivated) from Atlanta Biologicals; RNA-STAT 60 from Tel-Test, Inc.; mevalonolactone (M4667), puromycin dihydrochloride (P8833), oleic acid-albumin (O3008), crystal violet (C3886), cholesterol (C3045), 25-hydroxycholesterol (H1015), and lipoprotein-deficient serum (LPDS; S5394) from Sigma-Aldrich; site-1 protease inhibitor PF-429242 from Shanghai API Chemicals (947303-87-9); cell culture medium high glucose DMEM (10-013), DMEM/F12 (10-092), and penicillin-streptomycin (30-002) from Corning Cellgro; FuGENE 6 and RNase-free DNase I (10104159001) from Roche Applied Science; random primer mix (S1330), M-MuLV reverse transcriptase (M0253L), murine RNase inhibitor (M0314L), and oligo d(T)23VN (S1327S) from New England Biolabs; GoTaq real-time PCR mix (A6002) and CellTiter 96 NonRadioactive cell proliferation kit (G4000) from Promega. .. Stock solutions of fatostatin (20 mM) and PF-429242 (50 mM) were made by dissolving chemicals in DMSO.

    Concentration Assay:

    Article Title: Molecular characterization of human anti-hinge antibodies derived from single-cell cloning of normal human B cells
    Article Snippet: .. To generate first strand cDNA synthesis, each well was adjusted to contain a final concentration of 0.24 μ m hexamers and anchored dT primer mixture (Random Primer Mix S1330S, New England Biolabs, Ipswich, MA) and 7.7 units/μl of Superscript III (Thermo Fisher Scientific, Waltham, MA). .. The plates were incubated for three continuous 30-min intervals at 45, 50, and 55 °C.

    Quantitative RT-PCR:

    Article Title: Xenopus: An alternative model system for identifying muco-active agents
    Article Snippet: .. Quantitative RT-PCR Total RNA was extracted using a PureLink RNA mini kit (Invitrogen 12183018A), and the cDNA was synthesized using a random primer (NEB S1330S) and GoScript Reverse Transcriptase (Promega A50047). .. Quantitative RT-PCR was performed using the LightCycler 480 instrument (Roche LC480).

    Article Title: VIL1, a Polycomb-associated protein, modulates high ambient temperature response via H3K27me3 and H2A.Z in Arabidopsis thaliana
    Article Snippet: .. After DNaseI treatment to remove any DNA contamination, random primer mix (NEB #S1330S) and M-MLV Reverse transcriptase (Invitrogen #28025-013) were used for first strand synthesis. qRT-PCR was used to quantify the RNA prepared from transient expression experiments. .. AzuraQuant qPCR Master Mix (Azura Genomics) was used with initial incubation at 95°C for 2 min followed by 40 cycles of 95°C for 10 sec and 60°C for 30sec.

    Expressing:

    Article Title: VIL1, a Polycomb-associated protein, modulates high ambient temperature response via H3K27me3 and H2A.Z in Arabidopsis thaliana
    Article Snippet: .. After DNaseI treatment to remove any DNA contamination, random primer mix (NEB #S1330S) and M-MLV Reverse transcriptase (Invitrogen #28025-013) were used for first strand synthesis. qRT-PCR was used to quantify the RNA prepared from transient expression experiments. .. AzuraQuant qPCR Master Mix (Azura Genomics) was used with initial incubation at 95°C for 2 min followed by 40 cycles of 95°C for 10 sec and 60°C for 30sec.

    Polymerase Chain Reaction:

    Article Title: Fast-Seq: A Simple Method for Rapid and Inexpensive Validation of Packaged Single-Stranded Adeno-Associated Viral Genomes in Academic Settings
    Article Snippet: .. Reagent list: name (catalog no., vendor) Ethanol 200 proof (E7023; Sigma Aldrich) 5 M NaCl solution (AM9759; Thermo Fisher) HEPES 1 M (15630106; Thermo Fisher) KOH 8 N (P4494; Millipore Sigma) 0.5 M EDTA solution (15575020; Thermo Fisher) Dithiothreitol (R0861; Thermo Fisher) Triton X-100 (85112; Thermo Fisher) Glycerol (17904; Thermo Fisher) 1 M Tris pH 7.0 (AM9850G; Thermo Fisher) 1 M MgCl2 solution (AM9530G; Thermo Fisher) NN-dimethylformamide (D4551; Millipore Sigma) KAPA HiFi PCR kit with dNTPs (NC0142652; Thermo Fisher) 10% SDS (15553027; Thermo Fisher) i5/i7 index primers (any oligo provider) DNA LoBind tubes (13-698-791; Thermo Fisher) Protein LoBind tubes (0030108116; Eppendorf) SPRIselect (B23317; Beckman Coulter) Tn5 transposase with Nextera adapters (custom; QB3 Macrolab) Elution buffer (19086; Qiagen) DNase endonuclease (DB0715K; Lucigen) DNase exonuclease (E3101K; Lucigen) MinElute Virus Spin kit (57704; Qiagen) dNTP solution mix (N0447S; NEB) Klenow (M0210L; NEB) Millex-GP filter unit 0.22 μm (SLGP033RS; EMD) Random primer mix (S1330S; NEB) dNTP solution mix (N0447S; NEB) Ultrapure (10977015; Thermo Fisher) Qubit dsDNA High Sensitivity Kit (Q33230; Thermo Fisher). .. Equipment list Magnetic tube rack for microcentrifuge tubes Heat block Microcentrifuge Vortexer Benchtop centrifuge PCR thermocycler Qubit fluorometer with provided tubes Computer with 2GB+ of RAM, with Docker and Python 3.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    New England Biolabs random primer mix 6 nmol
    Random Primer Mix 6 Nmol, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/random primer mix 6 nmol/product/New England Biolabs
    Average 95 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    random primer mix 6 nmol - by Bioz Stars, 2020-09
    95/100 stars
      Buy from Supplier

    Image Search Results