Structured Review

Horizon Discovery raf1 sirna
cells were placed in suspension, or adherent cultures were serum-starved for two hours seventy-two hours post transfection with control or <t>Raf1</t> <t>siRNA.</t> Adherent cultures were then stimulated with EGF (1 ng/mL for 5 min) or plated on fibronectin (FN)-coated dishes for the indicated times. Cell lysates were analyzed by western blot with the indicated antisera.
Raf1 Sirna, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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85/100 stars

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Images

1) Product Images from "MEK1 activation by PAK: A novel mechanism"

Article Title: MEK1 activation by PAK: A novel mechanism

Journal: Cellular signalling

doi: 10.1016/j.cellsig.2007.01.018

cells were placed in suspension, or adherent cultures were serum-starved for two hours seventy-two hours post transfection with control or Raf1 siRNA. Adherent cultures were then stimulated with EGF (1 ng/mL for 5 min) or plated on fibronectin (FN)-coated dishes for the indicated times. Cell lysates were analyzed by western blot with the indicated antisera.
Figure Legend Snippet: cells were placed in suspension, or adherent cultures were serum-starved for two hours seventy-two hours post transfection with control or Raf1 siRNA. Adherent cultures were then stimulated with EGF (1 ng/mL for 5 min) or plated on fibronectin (FN)-coated dishes for the indicated times. Cell lysates were analyzed by western blot with the indicated antisera.

Techniques Used: Transfection, Western Blot

Related Articles

Transfection:

Article Title: PLX4032, a selective BRAFV600E kinase inhibitor, activates the ERK pathway and enhances cell migration and proliferation of BRAFWT melanoma cells
Article Snippet: .. RAF1 suppression by siRNA YUFIC melanoma cells were transfected with RAF1 siRNA (Smart pool siRNA; Dharmacon Inc; Chicago, IL, USA, Cat # L-087699-00), or Alexa Fluor 488 siRNA (100 nM/each) employing the DharmaFECT transfection reagent. .. At the end of 48 h, the cells were treated with PLX4032 or DMSO for 2 h, harvested and processed for Western blot analysis as above.

Article Title: MEK1 activation by PAK: A novel mechanism
Article Snippet: .. Cells were transfected with 100 nM control or Raf1 siRNA (SMARTpool, Dharmacon) using 30 μL Lipofectamine 2000 (Invitrogen). ..