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af555 conjugated goat anti rabbit secondary iggs  (Thermo Fisher)


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    Structured Review

    Thermo Fisher af555 conjugated goat anti rabbit secondary iggs
    Af555 Conjugated Goat Anti Rabbit Secondary Iggs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+secondary/Bovine+Serum+Albumin/pm42340982-232-19-24
    Average 99 stars, based on 1 article reviews
    af555 conjugated goat anti rabbit secondary iggs - by Bioz Stars, 2026-09
    99/100 stars

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    Incubation:

    Article Title: Defective DNA damage repair leads to frequent catastrophic genomic events in murine and human tumors
    Article Snippet: Slides were then washed with PBS for 5 min and then blocked with blocking solution (10% goat serum diluted in PBS, 0.2% Triton X-100) for 1 h. Slides were then incubated with primary antibodies, gh2Ax (Cell Signaling, 2577S) 53BP1 (Santa Cruz, sc-22760), Ki67 (Abcam, 15580), all at 1:100 dilution overnight at 4 °C. .. The next day slides were washed three times with PBS for 10 min each and incubated for an hour at room temperature with rabbit secondary (Invitrogen, Alexa 488, A11034 or Life Technology, Alexa 568, A11036) at 1:1000 dilution. .. After the incubation, slides were washed with PBS three times for 10 min each and then were mounted with DAPI Fluromount (Southern Biotech, 0100–020) and image analysis of the mouse cerebellum was done with Axio Zeiss Imager.M2 microscope.



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