rabbit polyclonal antinitrotyrosine ab  (Thermo Fisher)


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    Name:
    SuperSignal Enhanced Molecular Weight Protein Ladder
    Description:
    Thermo Scientific SuperSignal Enhanced Molecular Weight Protein Ladder 20 to 150K are a ready to use mix of recombinant proteins with IgG binding sites for chemiluminescent fluorescent chromogenic or other detection systems Features of SuperSignal Enhanced Molecular Weight Protein Ladder • Compatible enhanced version for compatibility with mouse monoclonal primary antibodies • Ready to use liquid format is ready to load and requires no boiling • Direct visualization see marker bands with gel stains and blotting detection reagents • Consistent even band development regardless of detection method • Stable store long term at 20°C three months at 4°C or one month at ambient SuperSignal Enhanced Molecular Weight Protein Ladder contain ready to use stabilized mixtures of eight recombinant proteins ranging in size from 20 to 150kDa These recombinant proteins bind antibodies used in the Western blot through an IgG binding site The protein markers can then be visualized either using appropriate substrates for enzyme labeled antibodies or via fluorescent dye labeled antibodies The MW marker mixes also contain a special pink tracking dye to monitor the electrophoresis run and membrane transfer without the need for additional colored markers The standards are compatible with nitrocellulose and PVDF membranes multiple blocking buffers and many other detection methods SuperSignal Molecular Weight Protein Ladders provide reliable and proportional band intensities in stained gels and immunoblots developed with chemiluminescent fluorescent chromogenic or other detection systems Two versions of the MW marker are offered The regular formulation is appropriate for use with most rabbit and other non mouse polyclonal antibodies The enhanced ladder is formulated specifically for applications requiring mouse monoclonal antibodies and experiments where very dilute antibody concentrations are used For example the enhanced standard may be necessary when using a 1 50 000 25 ng mL to 1 100 000 10 ng mL dilution of secondary antibody with SuperSignal West Pico Chemiluminescent substrate SuperSignal Molecular Weight Protein Ladders have flexible storage conditions depending on how often they are used For intermittent use store the molecular weight marker mix at 20°C For labs performing gel electrophoresis on a routine basis the protein standards can be kept at ambient temperature on the lab bench for one month or stored at 4°C for three months Related Products SuperSignal Molecular Weight Protein Ladder
    Catalog Number:
    84786
    Price:
    None
    Applications:
    Protein Assays and Analysis|Protein Biology|Protein Gel Electrophoresis|Western Blotting
    Category:
    Standards Ladders Controls
    Buy from Supplier


    Structured Review

    Thermo Fisher rabbit polyclonal antinitrotyrosine ab
    Thermo Scientific SuperSignal Enhanced Molecular Weight Protein Ladder 20 to 150K are a ready to use mix of recombinant proteins with IgG binding sites for chemiluminescent fluorescent chromogenic or other detection systems Features of SuperSignal Enhanced Molecular Weight Protein Ladder • Compatible enhanced version for compatibility with mouse monoclonal primary antibodies • Ready to use liquid format is ready to load and requires no boiling • Direct visualization see marker bands with gel stains and blotting detection reagents • Consistent even band development regardless of detection method • Stable store long term at 20°C three months at 4°C or one month at ambient SuperSignal Enhanced Molecular Weight Protein Ladder contain ready to use stabilized mixtures of eight recombinant proteins ranging in size from 20 to 150kDa These recombinant proteins bind antibodies used in the Western blot through an IgG binding site The protein markers can then be visualized either using appropriate substrates for enzyme labeled antibodies or via fluorescent dye labeled antibodies The MW marker mixes also contain a special pink tracking dye to monitor the electrophoresis run and membrane transfer without the need for additional colored markers The standards are compatible with nitrocellulose and PVDF membranes multiple blocking buffers and many other detection methods SuperSignal Molecular Weight Protein Ladders provide reliable and proportional band intensities in stained gels and immunoblots developed with chemiluminescent fluorescent chromogenic or other detection systems Two versions of the MW marker are offered The regular formulation is appropriate for use with most rabbit and other non mouse polyclonal antibodies The enhanced ladder is formulated specifically for applications requiring mouse monoclonal antibodies and experiments where very dilute antibody concentrations are used For example the enhanced standard may be necessary when using a 1 50 000 25 ng mL to 1 100 000 10 ng mL dilution of secondary antibody with SuperSignal West Pico Chemiluminescent substrate SuperSignal Molecular Weight Protein Ladders have flexible storage conditions depending on how often they are used For intermittent use store the molecular weight marker mix at 20°C For labs performing gel electrophoresis on a routine basis the protein standards can be kept at ambient temperature on the lab bench for one month or stored at 4°C for three months Related Products SuperSignal Molecular Weight Protein Ladder
    https://www.bioz.com/result/rabbit polyclonal antinitrotyrosine ab/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal antinitrotyrosine ab - by Bioz Stars, 2021-01
    99/100 stars

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    Incubation:

    Article Title: MEK5/ERK5 activation regulates colon cancer stem-like cell properties
    Article Snippet: .. Following incubation with appropriate horseradish peroxidase-conjugated secondary antibodies (Bio-Rad Laboratories, CA, USA), the proteins of interest were detected by chemiluminescence using SuperSignal reagents (Pierce, Thermo Fisher Scientific), on a ChemiDoc XRS+ imaging system (Bio-Rad). ..

    Article Title: The Complex of Ciliary Neurotrophic Factor-Ciliary Neurotrophic Factor Receptor ? Up-Regulates Connexin43 and Intercellular Coupling in Astrocytes via the Janus Tyrosine Kinase/Signal Transducer and Activator of Transcription Pathway D⃞
    Article Snippet: .. The blots were then incubated in Supersignal (Pierce Endogen) and exposed to Kodak X-Omat x-ray film to visualize antibody binding. .. To normalize protein loading, membranes were stripped of antibodies using 2-mercaptoethanol (10 mM), SDS (2%), and Tris (62.5 mM, pH 6.7) for 30 min at 65°C and subsequently probed for glyceraldehyde-3-phosphate dehydrogenase (GAPDH; CedarLane Laboratories), STAT3 (Cell Signaling Technology), or ERK1 and ERK2 (Santa Cruz Biotechnology, Santa Cruz, CA).

    Binding Assay:

    Article Title: The Complex of Ciliary Neurotrophic Factor-Ciliary Neurotrophic Factor Receptor ? Up-Regulates Connexin43 and Intercellular Coupling in Astrocytes via the Janus Tyrosine Kinase/Signal Transducer and Activator of Transcription Pathway D⃞
    Article Snippet: .. The blots were then incubated in Supersignal (Pierce Endogen) and exposed to Kodak X-Omat x-ray film to visualize antibody binding. .. To normalize protein loading, membranes were stripped of antibodies using 2-mercaptoethanol (10 mM), SDS (2%), and Tris (62.5 mM, pH 6.7) for 30 min at 65°C and subsequently probed for glyceraldehyde-3-phosphate dehydrogenase (GAPDH; CedarLane Laboratories), STAT3 (Cell Signaling Technology), or ERK1 and ERK2 (Santa Cruz Biotechnology, Santa Cruz, CA).

    Imaging:

    Article Title: MEK5/ERK5 activation regulates colon cancer stem-like cell properties
    Article Snippet: .. Following incubation with appropriate horseradish peroxidase-conjugated secondary antibodies (Bio-Rad Laboratories, CA, USA), the proteins of interest were detected by chemiluminescence using SuperSignal reagents (Pierce, Thermo Fisher Scientific), on a ChemiDoc XRS+ imaging system (Bio-Rad). ..

    Article Title: Phytanic acid activates PPARα to promote beige adipogenic differentiation of preadipocytes
    Article Snippet: .. Protein bands were detected by SuperSignal West Dura Extended Duration Substrate (Thermo Scientific, Waltham, MA, USA) and images were captured by Amersham Imager 600 imaging system (GE Healthcare Life Sciences, Pittsburgh, PA, USA). .. Gray analysis was performed using image J software and the protein band density was normalized to the β-actin expression.

    Blocking Assay:

    Article Title: Sorafenib has potent antitumor activity against multiple myeloma in vitro, ex vivo, and in vivo in the 5T33MM mouse model.
    Article Snippet: .. After blocking in 5% non-fat dry milk in PBS for 1 hour and probing with a specific primary antibody and a horseradish peroxidase–conjugated secondary antibody, the protein bands were detected by chemiluminescence (Supersignal, Pierce) and X-ray film exposure (Kodak). .. Protein loading was normalized by using anti-GAPDH or antiactin antibodies.