Journal: The Indonesian Biomedical Journal
Article Title: Elephantopus scaber Linn. Leaf Extract Sensitizes Doxorubicin in Inducing Apoptosis in HSC-3 Tongue Cancer Cells through Inhibiting Survivin Activity at Thr34
doi: 10.18585/inabj.v16i4.3096
Figure Lengend Snippet: Figure 4. ESLE decreased the amount of p-Surv (Thr34) in HSC-3 cells in concentration-dependent manner. HSC-3 cells were treated with/without 25 nM YM155 or ESLE in various concentrations. The amount of p-Surv (Thr34) was measured using Western Blot, as outlined in Methods. *Statistical significance (p<0.05) was determined using Tukey’s post-hoc test when compared to the sham group.
Article Snippet: After blocking with 5% skim milk in Tris-buffered saline (TBS, 150 nM NaCl and 50 nM Tris-HCl, pH 7.4), the sheets were incubated with rabbit polyclonal anti-β-Actin (Cat. No. 4967; Cell Signaling, Danvers, MA, USA) and rabbit polyclonal anti-phospho-survivin (Thr34) (Cat. No. 8888; Cell Signaling) antibody.
Techniques: Concentration Assay, Western Blot