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rabbit polyclonal antibody against phospho erbb2  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit polyclonal antibody against phospho erbb2
    Figure 4. Age-associated decline of EGFR signaling in wound healing. (A) Schematic representation of wound healing experiments with young and aged mice. Details are described in Materials and methods. (B) Left: Western blots of EGFR, phosphorylated EGFR (pEGFR), <t>ErbB2,</t> and phosphorylated ErbB2 <t>(pErbB2).</t> Middle and right: Quantification of EGFR and pEGFR expression normalized by GAPDH and EGFR, respectively. (C) Immunostaining with anti-EGFR and anti-pEGFR in infant and aged human keratinocytes with or without EGF treatment. Scale bar, 20 µm. (D) EGF-induced colony expansion of infant and aged human keratinocytes. White dotted lines indicate the periphery of keratinocyte colonies. Scale bar, 200 µm. (E) Relative increase in area of expanding colonies of keratinocytes isolated from infants and aged patients after the addition of EGF. (F) Distribution of colony types after 6 d of cultivation with or without EGF in cultures of infant and aged keratinocytes. The ages of patients from whom keratinocytes were isolated are indicated in each graph. P values were calculated with the two-tailed Student’s t test
    Rabbit Polyclonal Antibody Against Phospho Erbb2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 216 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal antibody against phospho erbb2/product/Cell Signaling Technology Inc
    Average 94 stars, based on 216 article reviews
    rabbit polyclonal antibody against phospho erbb2 - by Bioz Stars, 2026-06
    94/100 stars

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    1) Product Images from "EGFR-mediated epidermal stem cell motility drives skin regeneration through COL17A1 proteolysis."

    Article Title: EGFR-mediated epidermal stem cell motility drives skin regeneration through COL17A1 proteolysis.

    Journal: The Journal of cell biology

    doi: 10.1083/jcb.202012073

    Figure 4. Age-associated decline of EGFR signaling in wound healing. (A) Schematic representation of wound healing experiments with young and aged mice. Details are described in Materials and methods. (B) Left: Western blots of EGFR, phosphorylated EGFR (pEGFR), ErbB2, and phosphorylated ErbB2 (pErbB2). Middle and right: Quantification of EGFR and pEGFR expression normalized by GAPDH and EGFR, respectively. (C) Immunostaining with anti-EGFR and anti-pEGFR in infant and aged human keratinocytes with or without EGF treatment. Scale bar, 20 µm. (D) EGF-induced colony expansion of infant and aged human keratinocytes. White dotted lines indicate the periphery of keratinocyte colonies. Scale bar, 200 µm. (E) Relative increase in area of expanding colonies of keratinocytes isolated from infants and aged patients after the addition of EGF. (F) Distribution of colony types after 6 d of cultivation with or without EGF in cultures of infant and aged keratinocytes. The ages of patients from whom keratinocytes were isolated are indicated in each graph. P values were calculated with the two-tailed Student’s t test
    Figure Legend Snippet: Figure 4. Age-associated decline of EGFR signaling in wound healing. (A) Schematic representation of wound healing experiments with young and aged mice. Details are described in Materials and methods. (B) Left: Western blots of EGFR, phosphorylated EGFR (pEGFR), ErbB2, and phosphorylated ErbB2 (pErbB2). Middle and right: Quantification of EGFR and pEGFR expression normalized by GAPDH and EGFR, respectively. (C) Immunostaining with anti-EGFR and anti-pEGFR in infant and aged human keratinocytes with or without EGF treatment. Scale bar, 20 µm. (D) EGF-induced colony expansion of infant and aged human keratinocytes. White dotted lines indicate the periphery of keratinocyte colonies. Scale bar, 200 µm. (E) Relative increase in area of expanding colonies of keratinocytes isolated from infants and aged patients after the addition of EGF. (F) Distribution of colony types after 6 d of cultivation with or without EGF in cultures of infant and aged keratinocytes. The ages of patients from whom keratinocytes were isolated are indicated in each graph. P values were calculated with the two-tailed Student’s t test

    Techniques Used: Western Blot, Expressing, Immunostaining, Isolation, Two Tailed Test



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    rabbit polyclonal antibody against phospho erbb2  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc rabbit polyclonal antibody against phospho erbb2
    Figure 4. Age-associated decline of EGFR signaling in wound healing. (A) Schematic representation of wound healing experiments with young and aged mice. Details are described in Materials and methods. (B) Left: Western blots of EGFR, phosphorylated EGFR (pEGFR), <t>ErbB2,</t> and phosphorylated ErbB2 <t>(pErbB2).</t> Middle and right: Quantification of EGFR and pEGFR expression normalized by GAPDH and EGFR, respectively. (C) Immunostaining with anti-EGFR and anti-pEGFR in infant and aged human keratinocytes with or without EGF treatment. Scale bar, 20 µm. (D) EGF-induced colony expansion of infant and aged human keratinocytes. White dotted lines indicate the periphery of keratinocyte colonies. Scale bar, 200 µm. (E) Relative increase in area of expanding colonies of keratinocytes isolated from infants and aged patients after the addition of EGF. (F) Distribution of colony types after 6 d of cultivation with or without EGF in cultures of infant and aged keratinocytes. The ages of patients from whom keratinocytes were isolated are indicated in each graph. P values were calculated with the two-tailed Student’s t test
    Rabbit Polyclonal Antibody Against Phospho Erbb2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal antibody against phospho erbb2/product/Cell Signaling Technology Inc
    Average 94 stars, based on 1 article reviews
    rabbit polyclonal antibody against phospho erbb2 - by Bioz Stars, 2026-06
    94/100 stars
    		  Buy from Supplier

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    Figure 4. Age-associated decline of EGFR signaling in wound healing. (A) Schematic representation of wound healing experiments with young and aged mice. Details are described in Materials and methods. (B) Left: Western blots of EGFR, phosphorylated EGFR (pEGFR), ErbB2, and phosphorylated ErbB2 (pErbB2). Middle and right: Quantification of EGFR and pEGFR expression normalized by GAPDH and EGFR, respectively. (C) Immunostaining with anti-EGFR and anti-pEGFR in infant and aged human keratinocytes with or without EGF treatment. Scale bar, 20 µm. (D) EGF-induced colony expansion of infant and aged human keratinocytes. White dotted lines indicate the periphery of keratinocyte colonies. Scale bar, 200 µm. (E) Relative increase in area of expanding colonies of keratinocytes isolated from infants and aged patients after the addition of EGF. (F) Distribution of colony types after 6 d of cultivation with or without EGF in cultures of infant and aged keratinocytes. The ages of patients from whom keratinocytes were isolated are indicated in each graph. P values were calculated with the two-tailed Student’s t test

    Journal: The Journal of cell biology

    Article Title: EGFR-mediated epidermal stem cell motility drives skin regeneration through COL17A1 proteolysis.

    doi: 10.1083/jcb.202012073

    Figure Lengend Snippet: Figure 4. Age-associated decline of EGFR signaling in wound healing. (A) Schematic representation of wound healing experiments with young and aged mice. Details are described in Materials and methods. (B) Left: Western blots of EGFR, phosphorylated EGFR (pEGFR), ErbB2, and phosphorylated ErbB2 (pErbB2). Middle and right: Quantification of EGFR and pEGFR expression normalized by GAPDH and EGFR, respectively. (C) Immunostaining with anti-EGFR and anti-pEGFR in infant and aged human keratinocytes with or without EGF treatment. Scale bar, 20 µm. (D) EGF-induced colony expansion of infant and aged human keratinocytes. White dotted lines indicate the periphery of keratinocyte colonies. Scale bar, 200 µm. (E) Relative increase in area of expanding colonies of keratinocytes isolated from infants and aged patients after the addition of EGF. (F) Distribution of colony types after 6 d of cultivation with or without EGF in cultures of infant and aged keratinocytes. The ages of patients from whom keratinocytes were isolated are indicated in each graph. P values were calculated with the two-tailed Student’s t test

    Article Snippet: Primary antibodies used for Western blotting were as follows: rabbit monoclonal antibody against EGFR (Abcam; ab52894, 1:1,000), rabbit polyclonal antibody against phospho-EGFR (Tyr845; Millipore; 07-820, 1:1,000), rabbit polyclonal antibody against ErbB2 (Cell Signaling Technology; 2165, 1:1,000), rabbit polyclonal antibody against phospho-ErbB2 (Tyr1248; Cell Signaling Technology; 2247, 1:1,000), mouse monoclonal antibody against COL17A1 (Covance; SIG-3780, 1:500), mouse monoclonal antibody against PLEC (Cosmo Bio; NU-01-PLC, 1:200), rabbit polyclonal antibody against ITGA6 (Abcam; ab97760, 1:1,000), rabbit polyclonal antibody against ITGB4 (Santa Cruz Biotechnology; sc-9090, 1:1,000), rabbit monoclonal antibody against ITGB1 (Abcam; ab179471, 1:1,000), rabbit polyclonal antibody against GAPDH (Cell Signaling Technology; 2118, 1:5,000), and mouse monoclonal antibody against TIMP1 (Thermo Fisher Scientific; MS-608, 1:250).

    Techniques: Western Blot, Expressing, Immunostaining, Isolation, Two Tailed Test