rabbit polyclonal antibody against cdk5  (Santa Cruz Biotechnology)

Journal: Cell Death & Disease

Article Title: LncRNA TTN-AS1 promotes migration, invasion, and epithelial mesenchymal transition of lung adenocarcinoma via sponging miR-142-5p to regulate CDK5

doi: 10.1038/s41419-019-1811-y

Figure Lengend Snippet: a The predicted sites in the CDK5 3′-UTR for binding miR-142-5p. b The Luciferase reporter assay of A549, and H1650 cells were transfected with the CDK5-3′-UTR-WT or CDK5-3′-UTR-MUT in the miR-142-5p binding sites. Differences were observed when miR-142-5p mimic was added, and miR-NC mimic was used as control. c The effect of miR-142-5p mimic on the expression of CDK5 in A549 and H1650 cells

Article Snippet: The rabbit polyclonal antibody against CDK5 (EP715Y, Abcam) was used for detection.

Techniques: Binding Assay, Luciferase, Reporter Assay, Transfection, Expressing

Journal: Cell Death & Disease

Article Title: LncRNA TTN-AS1 promotes migration, invasion, and epithelial mesenchymal transition of lung adenocarcinoma via sponging miR-142-5p to regulate CDK5

doi: 10.1038/s41419-019-1811-y

Figure Lengend Snippet: a The effects of TTN-AS1 knockdown on migration ability of A549 and H1650 cells, assayed with wound-healing experiment. b The effects of TTN-AS1 knockdown on invasion ability of A549 and H1650 cells, assayed with transwell experiment. miR-142-5p mimic or inhibitor was used for realizing the indirect effect of TTN-AS1 on CDK5. Corresponding miR-NC mimic or inhibitor was used as control. * P < 0.05

Article Snippet: The rabbit polyclonal antibody against CDK5 (EP715Y, Abcam) was used for detection.

Techniques: Migration

Journal: Cell Death & Disease

Article Title: LncRNA TTN-AS1 promotes migration, invasion, and epithelial mesenchymal transition of lung adenocarcinoma via sponging miR-142-5p to regulate CDK5

doi: 10.1038/s41419-019-1811-y

Figure Lengend Snippet: a The representative expression of CDK5. Example I is showing high expression of CDK5, and example II is showing low expression of CDK5. b The effect of miR-142-5p inhibitor on the expression of EMT-associated proteins in A549 and H1650 cells with TTN-AS1 knockdown. * P < 0.05

Article Snippet: The rabbit polyclonal antibody against CDK5 (EP715Y, Abcam) was used for detection.

Techniques: Expressing

Journal: Cell Death & Disease

Article Title: LncRNA TTN-AS1 promotes migration, invasion, and epithelial mesenchymal transition of lung adenocarcinoma via sponging miR-142-5p to regulate CDK5

doi: 10.1038/s41419-019-1811-y

Figure Lengend Snippet: Association between CDK5 expression with TTN-AS1 and miR-142-5p expression in LUAD tissues

Article Snippet: The rabbit polyclonal antibody against CDK5 (EP715Y, Abcam) was used for detection.

Techniques: Expressing

Journal: The Journal of Neuroscience

Article Title: Loss of Cyclin-Dependent Kinase 5 from Parvalbumin Interneurons Leads to Hyperinhibition, Decreased Anxiety, and Memory Impairment

doi: 10.1523/JNEUROSCI.0969-14.2015

Figure Lengend Snippet: Genetic ablation of Cdk5 specifically in PV interneurons. A, B, Immunoreactivity for PV and Cdk5 in the motor cortex in 3.5-month-old fCdk5 (control) and PVCre;fCdk5 (mutant) littermate mice. The expression of Cdk5 (green) is present in all PV-positive (red) cells (arrows) in control mice (A). The complete ablation of Cdk5 is observed in PV interneurons of the PVCre;fCdk5 mice (B). Scale bars, 15 μm. C, D, Immunoreactivity for PV interneurons in different brain areas and layers in the cortex in control and PVCre;fCdk5 mice. C, PV interneurons (red) in different layers of the motor cortex in control and PVCre;fCdk5 mice, with Cux I (green) immunoreactivity labeling cortical layers II–IV. Scale bars, 100 μm. D, Bar graphs represent the relative numbers of PV interneurons in the cingulate cortex, hippocampus, and different layers of the motor cortex in 3.5-month-old control and PVCre;fCdk5 littermate mice. E, Representative Western blot and related quantification of Cdk5 protein in control and PVCre;fCdk5 (red) brains. Black, Control mice; red, PVCre;fCdk5 mice; ns, not significant. p > 0.05, error bars indicate ±SEM.

Article Snippet: The following primary antibodies were used: PV-positive interneurons were identified with anti-PV rabbit polyclonal PV-25 antibody at 1:2000 [catalog #PV 25; Resource Research Identifier (RRID) AB10000344; Swant] and mouse monoclonal PV-235 antibody at 1:1000 (catalog #PV 235; RRID AB10000343; Swant); rabbit polyclonal antibody against Cux I at 1:200 (M 22; catalog #sc-13024; RRID AB2261231; Santa Cruz Biotechnology) was used as a cortical layer marker; and Cdk5 immunoreactivity was identified with C8 rabbit polyclonal antibody against Cdk5 at 1:100 (catalog #sc-173; RRID AB631224 , Santa Cruz Biotechnology).

Techniques: Mutagenesis, Expressing, Labeling, Western Blot