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rabbit polyclonal antibodies against cdk5  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit polyclonal antibodies against cdk5
    Rabbit Polyclonal Antibodies Against Cdk5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal antibodies against cdk5/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal antibodies against cdk5 - by Bioz Stars, 2024-11
    95/100 stars

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    Abcam rabbit polyclonal antibody against cdk5
    a The predicted sites in the <t>CDK5</t> 3′-UTR for binding miR-142-5p. b The Luciferase reporter assay of A549, and H1650 cells were transfected with the CDK5-3′-UTR-WT or CDK5-3′-UTR-MUT in the miR-142-5p binding sites. Differences were observed when miR-142-5p mimic was added, and miR-NC mimic was used as control. c The effect of miR-142-5p mimic on the expression of CDK5 in A549 and H1650 cells
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    Santa Cruz Biotechnology rabbit polyclonal antibody against cdk5
    a The predicted sites in the <t>CDK5</t> 3′-UTR for binding miR-142-5p. b The Luciferase reporter assay of A549, and H1650 cells were transfected with the CDK5-3′-UTR-WT or CDK5-3′-UTR-MUT in the miR-142-5p binding sites. Differences were observed when miR-142-5p mimic was added, and miR-NC mimic was used as control. c The effect of miR-142-5p mimic on the expression of CDK5 in A549 and H1650 cells
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    Cell Signaling Technology Inc rabbit polyclonal antibodies against cdk5
    a The predicted sites in the <t>CDK5</t> 3′-UTR for binding miR-142-5p. b The Luciferase reporter assay of A549, and H1650 cells were transfected with the CDK5-3′-UTR-WT or CDK5-3′-UTR-MUT in the miR-142-5p binding sites. Differences were observed when miR-142-5p mimic was added, and miR-NC mimic was used as control. c The effect of miR-142-5p mimic on the expression of CDK5 in A549 and H1650 cells
    Rabbit Polyclonal Antibodies Against Cdk5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal antibodies against cdk5/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal antibodies against cdk5 - by Bioz Stars, 2024-11
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    Santa Cruz Biotechnology c8 rabbit polyclonal antibody against cdk5
    Genetic ablation of <t>Cdk5</t> specifically in PV interneurons. A, B, Immunoreactivity for PV and Cdk5 in the motor cortex in 3.5-month-old fCdk5 (control) and PVCre;fCdk5 (mutant) littermate mice. The expression of Cdk5 (green) is present in all PV-positive (red) cells (arrows) in control mice (A). The complete ablation of Cdk5 is observed in PV interneurons of the PVCre;fCdk5 mice (B). Scale bars, 15 μm. C, D, Immunoreactivity for PV interneurons in different brain areas and layers in the cortex in control and PVCre;fCdk5 mice. C, PV interneurons (red) in different layers of the motor cortex in control and PVCre;fCdk5 mice, with Cux I (green) immunoreactivity labeling cortical layers II–IV. Scale bars, 100 μm. D, Bar graphs represent the relative numbers of PV interneurons in the cingulate cortex, hippocampus, and different layers of the motor cortex in 3.5-month-old control and PVCre;fCdk5 littermate mice. E, Representative Western blot and related quantification of <t>Cdk5</t> <t>protein</t> in control and PVCre;fCdk5 (red) brains. Black, Control mice; red, PVCre;fCdk5 mice; ns, not significant. p > 0.05, error bars indicate ±SEM.
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    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    c8 rabbit polyclonal antibody against cdk5 - by Bioz Stars, 2024-11
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    a The predicted sites in the CDK5 3′-UTR for binding miR-142-5p. b The Luciferase reporter assay of A549, and H1650 cells were transfected with the CDK5-3′-UTR-WT or CDK5-3′-UTR-MUT in the miR-142-5p binding sites. Differences were observed when miR-142-5p mimic was added, and miR-NC mimic was used as control. c The effect of miR-142-5p mimic on the expression of CDK5 in A549 and H1650 cells

    Journal: Cell Death & Disease

    Article Title: LncRNA TTN-AS1 promotes migration, invasion, and epithelial mesenchymal transition of lung adenocarcinoma via sponging miR-142-5p to regulate CDK5

    doi: 10.1038/s41419-019-1811-y

    Figure Lengend Snippet: a The predicted sites in the CDK5 3′-UTR for binding miR-142-5p. b The Luciferase reporter assay of A549, and H1650 cells were transfected with the CDK5-3′-UTR-WT or CDK5-3′-UTR-MUT in the miR-142-5p binding sites. Differences were observed when miR-142-5p mimic was added, and miR-NC mimic was used as control. c The effect of miR-142-5p mimic on the expression of CDK5 in A549 and H1650 cells

    Article Snippet: The rabbit polyclonal antibody against CDK5 (EP715Y, Abcam) was used for detection.

    Techniques: Binding Assay, Luciferase, Reporter Assay, Transfection, Expressing

    a The effects of TTN-AS1 knockdown on migration ability of A549 and H1650 cells, assayed with wound-healing experiment. b The effects of TTN-AS1 knockdown on invasion ability of A549 and H1650 cells, assayed with transwell experiment. miR-142-5p mimic or inhibitor was used for realizing the indirect effect of TTN-AS1 on CDK5. Corresponding miR-NC mimic or inhibitor was used as control. * P < 0.05

    Journal: Cell Death & Disease

    Article Title: LncRNA TTN-AS1 promotes migration, invasion, and epithelial mesenchymal transition of lung adenocarcinoma via sponging miR-142-5p to regulate CDK5

    doi: 10.1038/s41419-019-1811-y

    Figure Lengend Snippet: a The effects of TTN-AS1 knockdown on migration ability of A549 and H1650 cells, assayed with wound-healing experiment. b The effects of TTN-AS1 knockdown on invasion ability of A549 and H1650 cells, assayed with transwell experiment. miR-142-5p mimic or inhibitor was used for realizing the indirect effect of TTN-AS1 on CDK5. Corresponding miR-NC mimic or inhibitor was used as control. * P < 0.05

    Article Snippet: The rabbit polyclonal antibody against CDK5 (EP715Y, Abcam) was used for detection.

    Techniques: Migration

    a The representative expression of CDK5. Example I is showing high expression of CDK5, and example II is showing low expression of CDK5. b The effect of miR-142-5p inhibitor on the expression of EMT-associated proteins in A549 and H1650 cells with TTN-AS1 knockdown. * P < 0.05

    Journal: Cell Death & Disease

    Article Title: LncRNA TTN-AS1 promotes migration, invasion, and epithelial mesenchymal transition of lung adenocarcinoma via sponging miR-142-5p to regulate CDK5

    doi: 10.1038/s41419-019-1811-y

    Figure Lengend Snippet: a The representative expression of CDK5. Example I is showing high expression of CDK5, and example II is showing low expression of CDK5. b The effect of miR-142-5p inhibitor on the expression of EMT-associated proteins in A549 and H1650 cells with TTN-AS1 knockdown. * P < 0.05

    Article Snippet: The rabbit polyclonal antibody against CDK5 (EP715Y, Abcam) was used for detection.

    Techniques: Expressing

    Association between  CDK5  expression with TTN-AS1 and miR-142-5p expression in LUAD tissues

    Journal: Cell Death & Disease

    Article Title: LncRNA TTN-AS1 promotes migration, invasion, and epithelial mesenchymal transition of lung adenocarcinoma via sponging miR-142-5p to regulate CDK5

    doi: 10.1038/s41419-019-1811-y

    Figure Lengend Snippet: Association between CDK5 expression with TTN-AS1 and miR-142-5p expression in LUAD tissues

    Article Snippet: The rabbit polyclonal antibody against CDK5 (EP715Y, Abcam) was used for detection.

    Techniques: Expressing

    Genetic ablation of Cdk5 specifically in PV interneurons. A, B, Immunoreactivity for PV and Cdk5 in the motor cortex in 3.5-month-old fCdk5 (control) and PVCre;fCdk5 (mutant) littermate mice. The expression of Cdk5 (green) is present in all PV-positive (red) cells (arrows) in control mice (A). The complete ablation of Cdk5 is observed in PV interneurons of the PVCre;fCdk5 mice (B). Scale bars, 15 μm. C, D, Immunoreactivity for PV interneurons in different brain areas and layers in the cortex in control and PVCre;fCdk5 mice. C, PV interneurons (red) in different layers of the motor cortex in control and PVCre;fCdk5 mice, with Cux I (green) immunoreactivity labeling cortical layers II–IV. Scale bars, 100 μm. D, Bar graphs represent the relative numbers of PV interneurons in the cingulate cortex, hippocampus, and different layers of the motor cortex in 3.5-month-old control and PVCre;fCdk5 littermate mice. E, Representative Western blot and related quantification of Cdk5 protein in control and PVCre;fCdk5 (red) brains. Black, Control mice; red, PVCre;fCdk5 mice; ns, not significant. p > 0.05, error bars indicate ±SEM.

    Journal: The Journal of Neuroscience

    Article Title: Loss of Cyclin-Dependent Kinase 5 from Parvalbumin Interneurons Leads to Hyperinhibition, Decreased Anxiety, and Memory Impairment

    doi: 10.1523/JNEUROSCI.0969-14.2015

    Figure Lengend Snippet: Genetic ablation of Cdk5 specifically in PV interneurons. A, B, Immunoreactivity for PV and Cdk5 in the motor cortex in 3.5-month-old fCdk5 (control) and PVCre;fCdk5 (mutant) littermate mice. The expression of Cdk5 (green) is present in all PV-positive (red) cells (arrows) in control mice (A). The complete ablation of Cdk5 is observed in PV interneurons of the PVCre;fCdk5 mice (B). Scale bars, 15 μm. C, D, Immunoreactivity for PV interneurons in different brain areas and layers in the cortex in control and PVCre;fCdk5 mice. C, PV interneurons (red) in different layers of the motor cortex in control and PVCre;fCdk5 mice, with Cux I (green) immunoreactivity labeling cortical layers II–IV. Scale bars, 100 μm. D, Bar graphs represent the relative numbers of PV interneurons in the cingulate cortex, hippocampus, and different layers of the motor cortex in 3.5-month-old control and PVCre;fCdk5 littermate mice. E, Representative Western blot and related quantification of Cdk5 protein in control and PVCre;fCdk5 (red) brains. Black, Control mice; red, PVCre;fCdk5 mice; ns, not significant. p > 0.05, error bars indicate ±SEM.

    Article Snippet: The following primary antibodies were used: PV-positive interneurons were identified with anti-PV rabbit polyclonal PV-25 antibody at 1:2000 [catalog #PV 25; Resource Research Identifier (RRID) AB10000344; Swant] and mouse monoclonal PV-235 antibody at 1:1000 (catalog #PV 235; RRID AB10000343; Swant); rabbit polyclonal antibody against Cux I at 1:200 (M 22; catalog #sc-13024; RRID AB2261231; Santa Cruz Biotechnology) was used as a cortical layer marker; and Cdk5 immunoreactivity was identified with C8 rabbit polyclonal antibody against Cdk5 at 1:100 (catalog #sc-173; RRID AB631224 , Santa Cruz Biotechnology).

    Techniques: Mutagenesis, Expressing, Labeling, Western Blot