acc 072  (Alomone Labs)


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    Alomone Labs acc 072
    Acc 072, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    acc 072 - by Bioz Stars, 2023-01
    94/100 stars

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    acc 072  (Alomone Labs)


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    Alomone Labs acc 072
    Acc 072, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acc 072/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
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    acc 072 - by Bioz Stars, 2023-01
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    rabbit anti tpcn2 monoclonal antibodies  (Alomone Labs)


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    Alomone Labs rabbit anti tpcn2 monoclonal antibodies
    The primers used in the quantitative reverse transcription polymerase chain reaction.
    Rabbit Anti Tpcn2 Monoclonal Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti tpcn2 monoclonal antibodies/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
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    rabbit anti tpcn2 monoclonal antibodies - by Bioz Stars, 2023-01
    94/100 stars

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    1) Product Images from "Deficiency of two-pore segment channel 2 contributes to systemic lupus erythematosus via regulation of apoptosis and cell cycle"

    Article Title: Deficiency of two-pore segment channel 2 contributes to systemic lupus erythematosus via regulation of apoptosis and cell cycle

    Journal: Chinese Medical Journal

    doi: 10.1097/CM9.0000000000001893

    The primers used in the quantitative reverse transcription polymerase chain reaction.
    Figure Legend Snippet: The primers used in the quantitative reverse transcription polymerase chain reaction.

    Techniques Used:

    TPCN2 -knockdown inhibits the proliferation in SLE. (A) Expression level of TPCN2 in PBMCs of SLE cases ( n = 6) and healthy controls ( n = 7). ∗ P < 0.05, Student's t test. Knockdown of TPCN2 with two independent shRNA in Jurkat (B) and THP-1 (C) cells; the left panel represents the relative expression levels of TPCN2 assessed by qRT-PCR; the right panel represents protein expression level of TPCN2 that was detected by Western blotting. The data represent the mean ( n = 3) ± SD. ∗ P < 0.05, Student's t test. Cell viability of silencing TPCN2 in Jurkat (D) and THP-1 (E) cells were determined by CCK-8 assay. The results represent the means ( n = 5) ± SD. Significant differences were evaluated using Student's t test. ∗ P < 0.05. CCK-8: Cell count kit-8; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; NC: Normal control; PBMCs: Peripheral blood mononuclear cells; qRT-PCR: Quantitative reverse transcription polymerase chain reaction; SD: Standard deviation; Sh#1: shTPCN2#1; Sh#2: shTPCN2#2; shRNA: Short hairpin RNA; SLE: Systemic lupus erythematosus; TPCN2 : Two-pore segment channel 2.
    Figure Legend Snippet: TPCN2 -knockdown inhibits the proliferation in SLE. (A) Expression level of TPCN2 in PBMCs of SLE cases ( n = 6) and healthy controls ( n = 7). ∗ P < 0.05, Student's t test. Knockdown of TPCN2 with two independent shRNA in Jurkat (B) and THP-1 (C) cells; the left panel represents the relative expression levels of TPCN2 assessed by qRT-PCR; the right panel represents protein expression level of TPCN2 that was detected by Western blotting. The data represent the mean ( n = 3) ± SD. ∗ P < 0.05, Student's t test. Cell viability of silencing TPCN2 in Jurkat (D) and THP-1 (E) cells were determined by CCK-8 assay. The results represent the means ( n = 5) ± SD. Significant differences were evaluated using Student's t test. ∗ P < 0.05. CCK-8: Cell count kit-8; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; NC: Normal control; PBMCs: Peripheral blood mononuclear cells; qRT-PCR: Quantitative reverse transcription polymerase chain reaction; SD: Standard deviation; Sh#1: shTPCN2#1; Sh#2: shTPCN2#2; shRNA: Short hairpin RNA; SLE: Systemic lupus erythematosus; TPCN2 : Two-pore segment channel 2.

    Techniques Used: Expressing, shRNA, Quantitative RT-PCR, Western Blot, CCK-8 Assay, Cell Counting, Reverse Transcription Polymerase Chain Reaction, Standard Deviation

    Knockdown of TPCN2 induces apoptosis and cell-cycle arrest in SLE. Apoptosis was detected by flow cytometry with Annexin V and PI in jurkat- TPCN2 -knockdown cells (A) and THP-1- TPCN2 -knockdown cells (B). TPCN2 -knockdown induced G2/M cell-cycle arrest. The percentage of G2-M phase cells of Jurkat (C) and THP-1 (D) was assessed by flow cytometry. ∗ P < 0.05. NC: Normal control; PI: Propidium iodide; Sh#1: shTPCN2#1; Sh#2: shTPCN2#2; SLE: Systemic lupus erythematosus; TPCN2 : Two-pore segment channel 2.
    Figure Legend Snippet: Knockdown of TPCN2 induces apoptosis and cell-cycle arrest in SLE. Apoptosis was detected by flow cytometry with Annexin V and PI in jurkat- TPCN2 -knockdown cells (A) and THP-1- TPCN2 -knockdown cells (B). TPCN2 -knockdown induced G2/M cell-cycle arrest. The percentage of G2-M phase cells of Jurkat (C) and THP-1 (D) was assessed by flow cytometry. ∗ P < 0.05. NC: Normal control; PI: Propidium iodide; Sh#1: shTPCN2#1; Sh#2: shTPCN2#2; SLE: Systemic lupus erythematosus; TPCN2 : Two-pore segment channel 2.

    Techniques Used: Flow Cytometry

    RNA-seq analyses of the effect of TPCN2 -knockdown on the gene expression profile. (A) The heatmap shows differential expression genes in sh#1 and sh#2 (in comparison with NC group). (B) The differential statistics of DEGs in NC groups vs . sh#1/sh#2. Blue columnar represent up-regulated DEGs. Red column represents down-regulated DEGs. (C) GO classification analysis of DEGs. NC group vs . sh#2. Molecular function – blue; Cellular components – red; Biological process – green. (D) Top 20 enriched KEGG pathways after silencing TPCN2 in Jurkat. The x-axis is the enrichment score, and the y-axis is the enriched pathways. DEGs: Differentially expressed genes; GO: Gene ontology; KEGG: Kyoto encyclopedia of genes and genomes; NC: Normal control; RNA-seq: RNA sequencing; Sh#1: shTPCN2#1; Sh#2: shTPCN2#2; TPCN2 : Two-pore segment channel 2.
    Figure Legend Snippet: RNA-seq analyses of the effect of TPCN2 -knockdown on the gene expression profile. (A) The heatmap shows differential expression genes in sh#1 and sh#2 (in comparison with NC group). (B) The differential statistics of DEGs in NC groups vs . sh#1/sh#2. Blue columnar represent up-regulated DEGs. Red column represents down-regulated DEGs. (C) GO classification analysis of DEGs. NC group vs . sh#2. Molecular function – blue; Cellular components – red; Biological process – green. (D) Top 20 enriched KEGG pathways after silencing TPCN2 in Jurkat. The x-axis is the enrichment score, and the y-axis is the enriched pathways. DEGs: Differentially expressed genes; GO: Gene ontology; KEGG: Kyoto encyclopedia of genes and genomes; NC: Normal control; RNA-seq: RNA sequencing; Sh#1: shTPCN2#1; Sh#2: shTPCN2#2; TPCN2 : Two-pore segment channel 2.

    Techniques Used: RNA Sequencing Assay, Expressing

    Representative enriched pathways in high-risk shTPCN2#2 through GSEA analysis. GSEA results showed that the G2/M checkpoint (A), inflammatory response (B), complement (C), and PI3K-AKT-mTOR (D) pathways were enriched in the sh#2 expression group. Top panels indicate the enrichment scores for each gene. Bottom panels show the ranking metrics of each gene. Y-axis: ranking metric values; X-axis: ranks for all genes. (E) The mRNA expression of some DEGs in Jurkat cells were detected by qRT-PCR. The RNA was extracted from cells knocked down of TPCN2 with two independent shRNA. The results were shown as the mean ± SD from three independent experiments. ∗ P < 0.05. AKT: Protein kinase B; DEGs: Differentially expressed genes; GSEA: Gene set enrichment analysis; mTOR: Mechanistic target of rapamycin; NC: Normal control; NES: Normalized enrichment score; PI3K: Phosphatidylinositol 3-kinase; qRT-PCR: Quantitative reverse transcription polymerase chain reaction; SD: standard deviation; Sh#1: shTPCN2#1; Sh#2: shTPCN2#2; shRNA: Short hairpin RNA; TPCN2 : Two-pore segment channel 2.
    Figure Legend Snippet: Representative enriched pathways in high-risk shTPCN2#2 through GSEA analysis. GSEA results showed that the G2/M checkpoint (A), inflammatory response (B), complement (C), and PI3K-AKT-mTOR (D) pathways were enriched in the sh#2 expression group. Top panels indicate the enrichment scores for each gene. Bottom panels show the ranking metrics of each gene. Y-axis: ranking metric values; X-axis: ranks for all genes. (E) The mRNA expression of some DEGs in Jurkat cells were detected by qRT-PCR. The RNA was extracted from cells knocked down of TPCN2 with two independent shRNA. The results were shown as the mean ± SD from three independent experiments. ∗ P < 0.05. AKT: Protein kinase B; DEGs: Differentially expressed genes; GSEA: Gene set enrichment analysis; mTOR: Mechanistic target of rapamycin; NC: Normal control; NES: Normalized enrichment score; PI3K: Phosphatidylinositol 3-kinase; qRT-PCR: Quantitative reverse transcription polymerase chain reaction; SD: standard deviation; Sh#1: shTPCN2#1; Sh#2: shTPCN2#2; shRNA: Short hairpin RNA; TPCN2 : Two-pore segment channel 2.

    Techniques Used: Expressing, Quantitative RT-PCR, shRNA, Reverse Transcription Polymerase Chain Reaction, Standard Deviation

    rabbit anti tpc2  (Alomone Labs)


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    Alomone Labs rabbit anti tpc2
    Representative confocal images of wild-type (A) and pore-dead (B) <t>TPC2-GCaMP-mApple</t> constructs expressed in HUVEC. GCaMP fluorescence signal is elicited upon stimulation with the TPC2 activator TPC2-A1-N and signal intensity is depicted as a heatmap. (C) Comparison of representative time-resolved fluorescence ratios in TPC2-A1-N stimulated cells expressing either the active TPC2 (black trace) or the pore-dead TPC2 (grey trace). Note that ionomycin-induced unspecific elevation of cytosolic Ca 2+ levels was still observable in both cases.
    Rabbit Anti Tpc2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti tpc2/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
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    rabbit anti tpc2 - by Bioz Stars, 2023-01
    94/100 stars

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    1) Product Images from "Leukocyte adhesion is governed by endolysosomal two pore channel 2 (TPC2)"

    Article Title: Leukocyte adhesion is governed by endolysosomal two pore channel 2 (TPC2)

    Journal: bioRxiv

    doi: 10.1101/2021.09.28.462104

    Representative confocal images of wild-type (A) and pore-dead (B) TPC2-GCaMP-mApple constructs expressed in HUVEC. GCaMP fluorescence signal is elicited upon stimulation with the TPC2 activator TPC2-A1-N and signal intensity is depicted as a heatmap. (C) Comparison of representative time-resolved fluorescence ratios in TPC2-A1-N stimulated cells expressing either the active TPC2 (black trace) or the pore-dead TPC2 (grey trace). Note that ionomycin-induced unspecific elevation of cytosolic Ca 2+ levels was still observable in both cases.
    Figure Legend Snippet: Representative confocal images of wild-type (A) and pore-dead (B) TPC2-GCaMP-mApple constructs expressed in HUVEC. GCaMP fluorescence signal is elicited upon stimulation with the TPC2 activator TPC2-A1-N and signal intensity is depicted as a heatmap. (C) Comparison of representative time-resolved fluorescence ratios in TPC2-A1-N stimulated cells expressing either the active TPC2 (black trace) or the pore-dead TPC2 (grey trace). Note that ionomycin-induced unspecific elevation of cytosolic Ca 2+ levels was still observable in both cases.

    Techniques Used: Construct, Fluorescence, Expressing

    Ratiometric in vivo Ca 2+ measurements showing a decreased Ca 2+ release from endolysosomal stores of cells treated with the TPC2 inhibitor trans-Ned 19 (10 µM). Blocking lysosomal Ca 2+ reuptake with the VATPase inhibitor bafilomycin A1 (250 nM) was used to amplify Ca 2+ signals. (A) Representative ratiometric time course measurement of endolysosomal Ca 2+ release upon bafilomycin A1 stimulation of control cells and trans-Ned19 treated cells. (B) Quantification of Ca 2+ release of 24 cells per condition from four independent experiments. Ca 2+ response is depicted in relation to the control. Two-tailed Student’s t-test (*p<0,05) was performed on the raw data.
    Figure Legend Snippet: Ratiometric in vivo Ca 2+ measurements showing a decreased Ca 2+ release from endolysosomal stores of cells treated with the TPC2 inhibitor trans-Ned 19 (10 µM). Blocking lysosomal Ca 2+ reuptake with the VATPase inhibitor bafilomycin A1 (250 nM) was used to amplify Ca 2+ signals. (A) Representative ratiometric time course measurement of endolysosomal Ca 2+ release upon bafilomycin A1 stimulation of control cells and trans-Ned19 treated cells. (B) Quantification of Ca 2+ release of 24 cells per condition from four independent experiments. Ca 2+ response is depicted in relation to the control. Two-tailed Student’s t-test (*p<0,05) was performed on the raw data.

    Techniques Used: In Vivo, Blocking Assay, Two Tailed Test

    (A) CD63 LEL to WPB transport was followed in cells treated with the TPC-targeting compounds (upper panel) or transfected with either control siRNA or TPC1/2 siRNA (lower panel). WPB were detected via VWF staining (green) and were analyzed for the amount of transferred anti-CD63 antibodies (red). Nuclei were visualized by DAPI (grey). Boxed areas indicate the regions magnified in the insets. Scale bars, 20 µm. To quantify the amount of anti-CD63 antibodies on WPB, Manders’ colocalization coefficients (MCC) were calculated from z-stack images of individual cells. Data represent mean ± SEM of at least 45 cells of three independent experiments and were analyzed by one-way ANOVA followed by Dunnett’s multiple comparison test (n.s., not significant, *p<0,05, **p<0.01, ***p<0.001, ****p<0.0001). (B) HUVEC were treated with the TPC2 inhibitor trans-Ned 19 or the activator TPC2-A1-N. WPB exocytosis was induced with histamine, and the amount of released VWF was quantified by ELISA. Note that TPC2 activity levels had no significant impact on VWF release.
    Figure Legend Snippet: (A) CD63 LEL to WPB transport was followed in cells treated with the TPC-targeting compounds (upper panel) or transfected with either control siRNA or TPC1/2 siRNA (lower panel). WPB were detected via VWF staining (green) and were analyzed for the amount of transferred anti-CD63 antibodies (red). Nuclei were visualized by DAPI (grey). Boxed areas indicate the regions magnified in the insets. Scale bars, 20 µm. To quantify the amount of anti-CD63 antibodies on WPB, Manders’ colocalization coefficients (MCC) were calculated from z-stack images of individual cells. Data represent mean ± SEM of at least 45 cells of three independent experiments and were analyzed by one-way ANOVA followed by Dunnett’s multiple comparison test (n.s., not significant, *p<0,05, **p<0.01, ***p<0.001, ****p<0.0001). (B) HUVEC were treated with the TPC2 inhibitor trans-Ned 19 or the activator TPC2-A1-N. WPB exocytosis was induced with histamine, and the amount of released VWF was quantified by ELISA. Note that TPC2 activity levels had no significant impact on VWF release.

    Techniques Used: Transfection, Staining, Enzyme-linked Immunosorbent Assay, Activity Assay

    (A) Representative images of cell-surface P-selectin pools of control cells and cells treated with either trans-Ned 19 (10 µM) or TPC2-A1-N (10 µM). Dashed lines outline cells of interest. Bars, 20 µm. (B) Quantitative analysis of P-selectin surface signals confirmed the altered cell surface levels of P-selectin. Data represent mean ± SEM of at least 22 cells per condition from three independent experiments and were analyzed by one-way ANOVA followed by Dunnett’s multiple comparison test (n.s., not significant, ***p<0.001).
    Figure Legend Snippet: (A) Representative images of cell-surface P-selectin pools of control cells and cells treated with either trans-Ned 19 (10 µM) or TPC2-A1-N (10 µM). Dashed lines outline cells of interest. Bars, 20 µm. (B) Quantitative analysis of P-selectin surface signals confirmed the altered cell surface levels of P-selectin. Data represent mean ± SEM of at least 22 cells per condition from three independent experiments and were analyzed by one-way ANOVA followed by Dunnett’s multiple comparison test (n.s., not significant, ***p<0.001).

    Techniques Used:

    anti two pore calcium channel protein 2  (Alomone Labs)


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    Alomone Labs anti two pore calcium channel protein 2
    Anti Two Pore Calcium Channel Protein 2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94/100 stars

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    anti tpc2  (Alomone Labs)


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    Alomone Labs anti tpc2
    Levels of TPC1 and <t>TPC2</t> mRNA relative to β-actin mRNA ( A ). Western-blot of TPC1 and TPC2 proteins ( B ) isolated from rat aorta SMCs and relative levels of TPC1 and TPC2 ( C ). The values panel A are the means of four measurements in different SMCs preparation + SEM (* p < 0.01). In panel B, the typical image of Western blots from one of three different cell lysates is presented. (+) Marks hybridization in the presence of blocking peptide. In panel C, n = 3 and n.s.—not significant.
    Anti Tpc2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    anti tpc2 - by Bioz Stars, 2023-01
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    1) Product Images from "The Role of Two-Pore Channels in Norepinephrine-Induced [Ca 2+ ] i Rise in Rat Aortic Smooth Muscle Cells and Aorta Contraction"

    Article Title: The Role of Two-Pore Channels in Norepinephrine-Induced [Ca 2+ ] i Rise in Rat Aortic Smooth Muscle Cells and Aorta Contraction

    Journal: Cells

    doi: 10.3390/cells8101144

    Levels of TPC1 and TPC2 mRNA relative to β-actin mRNA ( A ). Western-blot of TPC1 and TPC2 proteins ( B ) isolated from rat aorta SMCs and relative levels of TPC1 and TPC2 ( C ). The values panel A are the means of four measurements in different SMCs preparation + SEM (* p < 0.01). In panel B, the typical image of Western blots from one of three different cell lysates is presented. (+) Marks hybridization in the presence of blocking peptide. In panel C, n = 3 and n.s.—not significant.
    Figure Legend Snippet: Levels of TPC1 and TPC2 mRNA relative to β-actin mRNA ( A ). Western-blot of TPC1 and TPC2 proteins ( B ) isolated from rat aorta SMCs and relative levels of TPC1 and TPC2 ( C ). The values panel A are the means of four measurements in different SMCs preparation + SEM (* p < 0.01). In panel B, the typical image of Western blots from one of three different cell lysates is presented. (+) Marks hybridization in the presence of blocking peptide. In panel C, n = 3 and n.s.—not significant.

    Techniques Used: Western Blot, Isolation, Hybridization, Blocking Assay

    Decrease in [Ca 2+ ] i rise in response to NE in SMCs transfected with siRNA against TPC1. ( A ) Kinetics of [Ca 2+ ] i rise in SMCs transfected with siRNA against TPC1 and nontarget siRNA. The curves from one of four transfection experiments are presented. Each point at the curves is an average of six parallel measurements. ( B ) Calcium responses to NE (100 μM) and angiotensin II (0.1 μM) in SMCs transfected with nontarget siRNA, and siRNA against TPC1 and against TPC2. [Ca 2+ ] i rise in SMCs transfected with nontarget siRNA is taken as 100%. The mean values + SEM from four independent transfection experiments with different SMCs preparations are presented. There is significant difference between the responses of SMCs transfected with anti-TPC1 siRNA and with nontarget (** p < 0.01) or anti-TPC2 siRNA (* p < 0.05).
    Figure Legend Snippet: Decrease in [Ca 2+ ] i rise in response to NE in SMCs transfected with siRNA against TPC1. ( A ) Kinetics of [Ca 2+ ] i rise in SMCs transfected with siRNA against TPC1 and nontarget siRNA. The curves from one of four transfection experiments are presented. Each point at the curves is an average of six parallel measurements. ( B ) Calcium responses to NE (100 μM) and angiotensin II (0.1 μM) in SMCs transfected with nontarget siRNA, and siRNA against TPC1 and against TPC2. [Ca 2+ ] i rise in SMCs transfected with nontarget siRNA is taken as 100%. The mean values + SEM from four independent transfection experiments with different SMCs preparations are presented. There is significant difference between the responses of SMCs transfected with anti-TPC1 siRNA and with nontarget (** p < 0.01) or anti-TPC2 siRNA (* p < 0.05).

    Techniques Used: Transfection

    pore channel segment 2 tpc2  (Alomone Labs)


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    Alomone Labs pore channel segment 2 tpc2
    Pore Channel Segment 2 Tpc2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pore channel segment 2 tpc2 - by Bioz Stars, 2023-01
    94/100 stars

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    rabbit anti tpc2  (Alomone Labs)


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    Alomone Labs rabbit anti tpc2
    Rabbit Anti Tpc2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti tpc2/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
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    anti tpc2  (Alomone Labs)


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    Alomone Labs anti tpc2
    Anti Tpc2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti tpc2/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
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    anti tpc2  (Alomone Labs)


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    Alomone Labs anti tpc2
    Anti Tpc2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti tpc2/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
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    anti tpc2  (Alomone Labs)


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    Alomone Labs anti tpc2
    Anti Tpc2, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti tpc2/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
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