rabbit polyclonal anti stmn1  (Biosynth Carbosynth)

Journal: Oncology Letters

Article Title: Siva 1 inhibits proliferation, migration and invasion by phosphorylating Stathmin in ovarian cancer cells

doi: 10.3892/ol.2017.6307

Figure Lengend Snippet: Sequences of real-time PCR primers.

Article Snippet: Subsequent to blocking with 5% skim milk (YILI, Hohhot, Inner Mongolia, China) at room temperature for 1 h, diluted by TBS Tween (TBST), the PVDF membrane was incubated with the following antibodies at 4°C overnight: rabbit anti-human anti-Siva 1 polyclonal antibody (dilution, 1:200; Santa Cruz Biotechnology, Inc., Dallas, TX, USA, cat. no., sc-48767), rabbit anti-human anti-cleaved caspase-3 polyclonal antibody (dilution, 1:1,000; Abcam, Cambridge, UK, cat. no., ab2302), goat anti-human anti-cleaved caspase-9 polyclonal antibody (dilution, 1:200; Santa Cruz Biotechnology, Inc., cat. no., sc-22182), rabbit anti-human anti-Bcl-2-like protein 4 (Bax) polyclonal antibody (dilution, 1:400; Boster, Hubei, Wuhan, China, cat. no., BA0315), rabbit anti-human anti-Bcl-2 polyclonal antibody (dilution, 1:400; Boster, cat. no., BA0412), rabbit anti-human anti-Stathmin polyclonal antibody (dilution, 1:500; Bioss Antibodies, Beijing, China, cat. no., bs-1902R), rabbit anti-human anti-phosphorylated Stathmin polyclonal antibody (dilution, 1:500; Bioss Antibodies, cat. no., bs-3431R), rabbit anti-human anti-α-tubulin polyclonal antibody (dilution, 1:1,000; Abcam, cat. no., ab178484) or mouse anti-human anti-acetyl-α-tubulin monoclonal antibody (dilution, 1:1,000; Abcam, cat. no., ab24610).

Techniques: Real-time Polymerase Chain Reaction, Sequencing

Journal: Oncology Letters

Article Title: Siva 1 inhibits proliferation, migration and invasion by phosphorylating Stathmin in ovarian cancer cells

doi: 10.3892/ol.2017.6307

Figure Lengend Snippet: Siva 1 enhances phosphorylation of Stathmin. (A) Siva 1 does not affect Stathmin mRNA level. (B and C) Overexpression of Siva 1 enhances phosphorylation of Stathmin and acetylation of α-tubulin, but did not markedly affect the expression level of Stathmin and α-tubulin detected by western blot. ***P<0.001 vs. pCMV group; ns, no significance.

Article Snippet: Subsequent to blocking with 5% skim milk (YILI, Hohhot, Inner Mongolia, China) at room temperature for 1 h, diluted by TBS Tween (TBST), the PVDF membrane was incubated with the following antibodies at 4°C overnight: rabbit anti-human anti-Siva 1 polyclonal antibody (dilution, 1:200; Santa Cruz Biotechnology, Inc., Dallas, TX, USA, cat. no., sc-48767), rabbit anti-human anti-cleaved caspase-3 polyclonal antibody (dilution, 1:1,000; Abcam, Cambridge, UK, cat. no., ab2302), goat anti-human anti-cleaved caspase-9 polyclonal antibody (dilution, 1:200; Santa Cruz Biotechnology, Inc., cat. no., sc-22182), rabbit anti-human anti-Bcl-2-like protein 4 (Bax) polyclonal antibody (dilution, 1:400; Boster, Hubei, Wuhan, China, cat. no., BA0315), rabbit anti-human anti-Bcl-2 polyclonal antibody (dilution, 1:400; Boster, cat. no., BA0412), rabbit anti-human anti-Stathmin polyclonal antibody (dilution, 1:500; Bioss Antibodies, Beijing, China, cat. no., bs-1902R), rabbit anti-human anti-phosphorylated Stathmin polyclonal antibody (dilution, 1:500; Bioss Antibodies, cat. no., bs-3431R), rabbit anti-human anti-α-tubulin polyclonal antibody (dilution, 1:1,000; Abcam, cat. no., ab178484) or mouse anti-human anti-acetyl-α-tubulin monoclonal antibody (dilution, 1:1,000; Abcam, cat. no., ab24610).

Techniques: Over Expression, Expressing, Western Blot

Journal: Molecular and Clinical Oncology

Article Title: Stathmin1 promotes lymph node metastasis in hypopharyngeal squamous cell carcinoma via regulation of HIF‑1α/VEGF‑A axis and MTA1 expression

doi: 10.3892/mco.2023.2617

Figure Lengend Snippet: Sequences of shRNAs targeting STMN1.

Article Snippet: The sections were subsequently incubated with rabbit anti-STMN1 polyclonal antibody (1:75) (Wuhan Boster Biological Technology, Ltd.), or rabbit anti-D2-40 polyclonal antibody (1:75) (Wuhan Boster Biological Technology, Ltd.) (Anti-D2-40 antibody was used to stain lymphatic vessels, and in stained lymphatic vessels, the presence of tumor embolus represented LVI) overnight at 4 ̊C.

Techniques: Sequencing, shRNA

Journal: Molecular and Clinical Oncology

Article Title: Stathmin1 promotes lymph node metastasis in hypopharyngeal squamous cell carcinoma via regulation of HIF‑1α/VEGF‑A axis and MTA1 expression

doi: 10.3892/mco.2023.2617

Figure Lengend Snippet: Primer sequences of genes analyzed for reverse transcription-quantitative PCR.

Article Snippet: The sections were subsequently incubated with rabbit anti-STMN1 polyclonal antibody (1:75) (Wuhan Boster Biological Technology, Ltd.), or rabbit anti-D2-40 polyclonal antibody (1:75) (Wuhan Boster Biological Technology, Ltd.) (Anti-D2-40 antibody was used to stain lymphatic vessels, and in stained lymphatic vessels, the presence of tumor embolus represented LVI) overnight at 4 ̊C.

Techniques:

Journal: Molecular and Clinical Oncology

Article Title: Stathmin1 promotes lymph node metastasis in hypopharyngeal squamous cell carcinoma via regulation of HIF‑1α/VEGF‑A axis and MTA1 expression

doi: 10.3892/mco.2023.2617

Figure Lengend Snippet: Clinicopathological characteristics of the included cases.

Article Snippet: The sections were subsequently incubated with rabbit anti-STMN1 polyclonal antibody (1:75) (Wuhan Boster Biological Technology, Ltd.), or rabbit anti-D2-40 polyclonal antibody (1:75) (Wuhan Boster Biological Technology, Ltd.) (Anti-D2-40 antibody was used to stain lymphatic vessels, and in stained lymphatic vessels, the presence of tumor embolus represented LVI) overnight at 4 ̊C.

Techniques: Expressing

Journal: Molecular and Clinical Oncology

Article Title: Stathmin1 promotes lymph node metastasis in hypopharyngeal squamous cell carcinoma via regulation of HIF‑1α/VEGF‑A axis and MTA1 expression

doi: 10.3892/mco.2023.2617

Figure Lengend Snippet: STMN1 expression differences and immunohistochemical staining for STMN1 and LVI. (A and B) STMN1 expression level was increased in HSCC tissues compared with normal hypopharyngeal tissues, and was further increased in HSCC tissues of patients with metastases. (C and D) STMN1 expression status in different HSCC samples (magnification, x200). (E and F) LVI statuses in different HSCC samples along with STMN1 expression statuses (magnification, x400). STMN1, stathmin1; LVI, lymphatic vessel invasion; HSCC, hypopharyngeal squamous cell carcinoma.

Article Snippet: The sections were subsequently incubated with rabbit anti-STMN1 polyclonal antibody (1:75) (Wuhan Boster Biological Technology, Ltd.), or rabbit anti-D2-40 polyclonal antibody (1:75) (Wuhan Boster Biological Technology, Ltd.) (Anti-D2-40 antibody was used to stain lymphatic vessels, and in stained lymphatic vessels, the presence of tumor embolus represented LVI) overnight at 4 ̊C.

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: Molecular and Clinical Oncology

Article Title: Stathmin1 promotes lymph node metastasis in hypopharyngeal squamous cell carcinoma via regulation of HIF‑1α/VEGF‑A axis and MTA1 expression

doi: 10.3892/mco.2023.2617

Figure Lengend Snippet: The transfection efficacy evaluation of STMN1 knockdown and the results of cell functional experiments. (A and B) The relative mRNA and protein expression levels of STMN1 in different groups. All of them were evidently decreased after STMN1 knockdown with different shRNA sequences, compared with the NC shRNA group. (C-E) The OD 450 , the relative migratory ratio at 48 h, and the relative number of invasive cells were all significantly decreased after STMN1 knockdown with different shRNA sequences; all were compared with the NC shRNA group. The magnification of picture D and picture E were 100 times and 200 times, respectively. * P<0.05 and ** P<0.01. STMN1, stathmin1; shRNA, short hairpin RNA; NC, negative control.

Article Snippet: The sections were subsequently incubated with rabbit anti-STMN1 polyclonal antibody (1:75) (Wuhan Boster Biological Technology, Ltd.), or rabbit anti-D2-40 polyclonal antibody (1:75) (Wuhan Boster Biological Technology, Ltd.) (Anti-D2-40 antibody was used to stain lymphatic vessels, and in stained lymphatic vessels, the presence of tumor embolus represented LVI) overnight at 4 ̊C.

Techniques: Transfection, Knockdown, Functional Assay, Expressing, shRNA, Negative Control

Journal: Molecular and Clinical Oncology

Article Title: Stathmin1 promotes lymph node metastasis in hypopharyngeal squamous cell carcinoma via regulation of HIF‑1α/VEGF‑A axis and MTA1 expression

doi: 10.3892/mco.2023.2617

Figure Lengend Snippet: Bioinformatics analyses for target genes and pathways of STMN1. (A) STMN1 was evidently enriched in the HIF-1α pathway in head and neck squamous cell carcinoma. (B) Gene expression levels of HIF-1α pathway were different between cases with high expression of STMN1 and those with low expression of STMN1. (C and D) Target gene prediction analysis from website https://www.aclbi.com , which demonstrated that the expression level of MTA1 was significantly correlated with that of STMN1. STMN1, stathmin1; HIF-1α, hypoxia inducible factor-1alpha; MTA1, tumor metastasis-associated protein 1.

Article Snippet: The sections were subsequently incubated with rabbit anti-STMN1 polyclonal antibody (1:75) (Wuhan Boster Biological Technology, Ltd.), or rabbit anti-D2-40 polyclonal antibody (1:75) (Wuhan Boster Biological Technology, Ltd.) (Anti-D2-40 antibody was used to stain lymphatic vessels, and in stained lymphatic vessels, the presence of tumor embolus represented LVI) overnight at 4 ̊C.

Techniques: Gene Expression, Expressing

Journal: Molecular and Clinical Oncology

Article Title: Stathmin1 promotes lymph node metastasis in hypopharyngeal squamous cell carcinoma via regulation of HIF‑1α/VEGF‑A axis and MTA1 expression

doi: 10.3892/mco.2023.2617

Figure Lengend Snippet: The mRNA and protein expression levels of the target genes of STMN1 in FaDu cells. (A-C) The relative mRNA and protein expression levels of HIF-1α were signifiicantly decreased after STMN1 knockdown with different shRNA sequences. (D) The relative expression level of VEGF-A, the downstream protein of HIF-1α, was significantly decreased after STMN1 knockdown. (E and F) Both relative mRNA and protein expression levels of MTA1 significantly decreased after STMN1 knockdown. All were compared with the NC shRNA group. * P<0.05, ** P<0.01 and *** P<0.001. STMN1, stathmin1; HIF-1α, hypoxia inducible factor-1alpha; shRNA, short hairpin RNA; VEGF, vascular endothelial growth factor; MTA1, tumor metastasis-associated protein 1; NC, negative control.

Article Snippet: The sections were subsequently incubated with rabbit anti-STMN1 polyclonal antibody (1:75) (Wuhan Boster Biological Technology, Ltd.), or rabbit anti-D2-40 polyclonal antibody (1:75) (Wuhan Boster Biological Technology, Ltd.) (Anti-D2-40 antibody was used to stain lymphatic vessels, and in stained lymphatic vessels, the presence of tumor embolus represented LVI) overnight at 4 ̊C.

Techniques: Expressing, Knockdown, shRNA, Negative Control

Journal: Gland Surgery

Article Title: The prognostic role of a phospho-Stathmin 1 signature in breast cancer treated with neoadjuvant chemotherapy

doi: 10.21037/gs-22-628

Figure Lengend Snippet: Relationship between STMN1 and its phosphorylation-related proteins and clinical outcomes for breast cancer patients. (A) Relationship between Kaplan-Meier analysis of DFS and STMN1 level by IHC (400×). (B) Relationship between Kaplan-Meier analysis of DFS and STMN1 phosphorylation at Ser16 levelby IHC (400×). (C) Relationship between Kaplan-Meier analysis of DFS and STMN1 phosphorylation at Ser25 levelby IHC (400×). (D) Relationship between Kaplan-Meier analysis of DFS and STMN1 phosphorylation at Ser38 levelby IHC (400×). (E) Relationship between Kaplan-Meier analysis of DFS and STMN1 phosphorylation at Ser63 levelby IHC (400×). (F) Relationship between Kaplan-Meier analysis of DFS and GRP78 level by IHC (400×). DFS, disease-free survival.

Article Snippet: Polyclonal rabbit anti-human STMN1 antibody (Proteintech) was diluted to 1:400 and incubated with samples in a humid chamber at 4 °C overnight.

Techniques: Phospho-proteomics

Journal: Gland Surgery

Article Title: The prognostic role of a phospho-Stathmin 1 signature in breast cancer treated with neoadjuvant chemotherapy

doi: 10.21037/gs-22-628

Figure Lengend Snippet: Relationship between STMN1 phosphorylation and pathologic response. (A) Correlation between pathologic response and STMN1 expression. (B) Correlation between pathologic response and STMN1 phosphorylation at Ser-16. (C) Correlation between pathologic response and STMN1 phosphorylation at Ser-25. (D) Correlation between pathologic response and STMN1 phosphorylation at Ser-38. (E) Correlation between pathologic response and STMN1 phosphorylation at Ser-63. (F) Correlation between pathologic response and GRP78. pCR, pathological complete response.

Article Snippet: Polyclonal rabbit anti-human STMN1 antibody (Proteintech) was diluted to 1:400 and incubated with samples in a humid chamber at 4 °C overnight.

Techniques: Phospho-proteomics, Expressing

Journal: Gland Surgery

Article Title: The prognostic role of a phospho-Stathmin 1 signature in breast cancer treated with neoadjuvant chemotherapy

doi: 10.21037/gs-22-628

Figure Lengend Snippet: p-STMN1/GRP78 model powerfully predicts DFS for patients treated with NACT. (A) Kaplan-Meier analysis of DFS in breast cancer patients from high and low-risk groups classified by the p-STMN1/GRP78 model. (B) ROC curves to test the prognostic accuracy of the p-STMN1/GRP78 model. DFS, disease-free survival; ROC, receiver operating characteristic; AUC, area under the receiver operating characteristic curve.

Article Snippet: Polyclonal rabbit anti-human STMN1 antibody (Proteintech) was diluted to 1:400 and incubated with samples in a humid chamber at 4 °C overnight.

Techniques:

Journal: Gland Surgery

Article Title: The prognostic role of a phospho-Stathmin 1 signature in breast cancer treated with neoadjuvant chemotherapy

doi: 10.21037/gs-22-628

Figure Lengend Snippet: Relationship between STMN1 and its phosphorylation-related proteins and clinical outcomes for breast cancer patients. (A) Relationship between Kaplan-Meier analysis of DFS and STMN1 level by IHC (400×). (B) Relationship between Kaplan-Meier analysis of DFS and STMN1 phosphorylation at Ser16 levelby IHC (400×). (C) Relationship between Kaplan-Meier analysis of DFS and STMN1 phosphorylation at Ser25 levelby IHC (400×). (D) Relationship between Kaplan-Meier analysis of DFS and STMN1 phosphorylation at Ser38 levelby IHC (400×). (E) Relationship between Kaplan-Meier analysis of DFS and STMN1 phosphorylation at Ser63 levelby IHC (400×). (F) Relationship between Kaplan-Meier analysis of DFS and GRP78 level by IHC (400×). DFS, disease-free survival.

Article Snippet: Polyclonal rabbit anti-human STMN1 Ser38 antibody (Cell Signaling Technologies) was diluted to 1:100 and incubated with samples in a humid chamber at 4 °C overnight. respectively.

Techniques: Phospho-proteomics

Journal: Gland Surgery

Article Title: The prognostic role of a phospho-Stathmin 1 signature in breast cancer treated with neoadjuvant chemotherapy

doi: 10.21037/gs-22-628

Figure Lengend Snippet: Relationship between STMN1 phosphorylation and pathologic response. (A) Correlation between pathologic response and STMN1 expression. (B) Correlation between pathologic response and STMN1 phosphorylation at Ser-16. (C) Correlation between pathologic response and STMN1 phosphorylation at Ser-25. (D) Correlation between pathologic response and STMN1 phosphorylation at Ser-38. (E) Correlation between pathologic response and STMN1 phosphorylation at Ser-63. (F) Correlation between pathologic response and GRP78. pCR, pathological complete response.

Article Snippet: Polyclonal rabbit anti-human STMN1 Ser38 antibody (Cell Signaling Technologies) was diluted to 1:100 and incubated with samples in a humid chamber at 4 °C overnight. respectively.

Techniques: Phospho-proteomics, Expressing

Journal: Gland Surgery

Article Title: The prognostic role of a phospho-Stathmin 1 signature in breast cancer treated with neoadjuvant chemotherapy

doi: 10.21037/gs-22-628

Figure Lengend Snippet: p-STMN1/GRP78 model powerfully predicts DFS for patients treated with NACT. (A) Kaplan-Meier analysis of DFS in breast cancer patients from high and low-risk groups classified by the p-STMN1/GRP78 model. (B) ROC curves to test the prognostic accuracy of the p-STMN1/GRP78 model. DFS, disease-free survival; ROC, receiver operating characteristic; AUC, area under the receiver operating characteristic curve.

Article Snippet: Polyclonal rabbit anti-human STMN1 Ser38 antibody (Cell Signaling Technologies) was diluted to 1:100 and incubated with samples in a humid chamber at 4 °C overnight. respectively.

Techniques:

Journal: Cell Reports

Article Title: Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals

doi: 10.1016/j.celrep.2022.110610

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-STMN1 , Proteintech , Cat# 11157-1-AP, RRID:AB_2197114.

Techniques: Recombinant, Software

Journal: International journal of oncology

Article Title: High stathmin 1 expression is associated with poor prognosis and chemoradiation resistance in esophageal squamous cell carcinoma.

doi: 10.3892/ijo.2017.3899

Figure Lengend Snippet: Figure 1. Analysis of the cytoplasmic staining of STMN1 and survival time in surgically resected ESCC. (A) Cytoplasmic STMN1 expression in ESCC and normal esophageal squamous mucosa. (B) High power view of (A). ESCCs with high STMN1 expression invaded the normal esophageal squamous mucosa. (C) Representative section from ESCC showing low STMN1 expression. (D) Representative section from ESCC showing high STMN1 expression. (E) Disease-specific 5-year survival rates for patients with high and low STMN1 expression in ESCC tumors (high, 49.5%; low, 73.9%; P=0.002). (F) Overall 5-year survival rates (high, 43.6%; low, 70.2%; P=0.001). Disease-specific and overall survival rates were significantly lower in ESCC patients with high STMN1 expression.

Article Snippet: Total protein was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PagE) using bis-Tris gels and was transferred to membranes. after membranes were blocked with 5% skim milk, STMN1 protein was detected by immunoblotting with anti-STMN1 rabbit polyclonal antibodies (Cell Signaling Technology, Danvers, Ma, USa) and vascular endothelial growth factor a (VEgF) with an anti-VEgF rabbit monoclonal antibody (Abcam, Cambridge, UK) diluted 1:1,000. an antiHSC70 antibody (1:500; Santa Cruz biotechnology) served as a gel loading control.

Techniques: Staining, Expressing

Journal: International journal of oncology

Article Title: High stathmin 1 expression is associated with poor prognosis and chemoradiation resistance in esophageal squamous cell carcinoma.

doi: 10.3892/ijo.2017.3899

Figure Lengend Snippet: Figure 2. Inverse association of STMN1 expression in pretreatment ESCC biopsy samples with therapeutic efficacy of docetaxel combination CRT. Immunohistochemistry showing the cytoplasmic staining of STMN1 in biopsy specimens before docetaxel combination CRT in (A) a patient with low STMN1 expression and (B) a patient with high STMN1 expression.

Article Snippet: Total protein was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PagE) using bis-Tris gels and was transferred to membranes. after membranes were blocked with 5% skim milk, STMN1 protein was detected by immunoblotting with anti-STMN1 rabbit polyclonal antibodies (Cell Signaling Technology, Danvers, Ma, USa) and vascular endothelial growth factor a (VEgF) with an anti-VEgF rabbit monoclonal antibody (Abcam, Cambridge, UK) diluted 1:1,000. an antiHSC70 antibody (1:500; Santa Cruz biotechnology) served as a gel loading control.

Techniques: Expressing, Drug discovery, Immunohistochemistry, Staining

Journal: International journal of oncology

Article Title: High stathmin 1 expression is associated with poor prognosis and chemoradiation resistance in esophageal squamous cell carcinoma.

doi: 10.3892/ijo.2017.3899

Figure Lengend Snippet: Figure 3. Knockdown of STMN1 expression sensitizes ESCCs to docetaxel and radiation. (A) Differential expression of STMN1 protein in HET1A, TE1, TE8, TE15 and KYSE140 cells as determined by western blotting. HSC70 expression was used as the gel loading control. (B) Western blot analysis showing a marked decrease in STMN1 and VEGF expression levels (band intensities) in lysates from TE-8 and KYSE140 cells transfected with STMN1-specific siRNA compared to those in lysates from untransfected cells and negative control siRNA transfectants. (C) Docetaxel sensitivity in TE8 and KYSE140 cells transfected with STMN1-specific siRNA was determined by WST-8 assay. STMN1-specific siRNA transfectants showed significantly increased sensitivity to docetaxel compared to control transfectants (*P<0.05; **P<0.01). Each point represents the mean ± SD of triplicate determinations from two independent experiments. (D) Radiation sensitivity in TE8 cells transfected with STMN1 siRNA was evaluated by the colony formation assay. The survival fraction of STMN1-suppressed cells was significantly reduced compared to that of control cells.

Article Snippet: Total protein was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PagE) using bis-Tris gels and was transferred to membranes. after membranes were blocked with 5% skim milk, STMN1 protein was detected by immunoblotting with anti-STMN1 rabbit polyclonal antibodies (Cell Signaling Technology, Danvers, Ma, USa) and vascular endothelial growth factor a (VEgF) with an anti-VEgF rabbit monoclonal antibody (Abcam, Cambridge, UK) diluted 1:1,000. an antiHSC70 antibody (1:500; Santa Cruz biotechnology) served as a gel loading control.

Techniques: Knockdown, Expressing, Quantitative Proteomics, Western Blot, Control, Transfection, Negative Control, Colony Assay