rabbit polyclonal anti sparc  (Cell Signaling Technology Inc)

Journal: Cell reports

Article Title: PAI-1 uncouples integrin-β1 from restrain by membrane-bound β-catenin to promote collagen fibril remodeling in obesity-related neoplasms

doi: 10.1016/j.celrep.2024.114527

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-SPARC , Cell Signaling Technology , Cat# 5420S; RRID: AB_10692794.

Techniques: Recombinant, Membrane, Cell Culture, Reverse Transcription, SYBR Green Assay, Clinical Proteomics, Protein Extraction, Extraction, Bicinchoninic Acid Protein Assay, In Situ, Blocking Assay, Migration, shRNA, Control, Software, Pyromark Assay, Western Blot, Simple Western

Journal: Journal of IMAB - Annual Proceeding (Scientific Papers)

Article Title: SUPPLEMENT 12 SEEC & 32 IMAB, Section Varia

doi: 10.5272/jimab.2022supplement3

Figure Lengend Snippet: Fig.1. An immunohistochemical method for monitoring the expression of the SPARC in femur preparations. Weak and strong expression of SPARC is shown in the osteoblasts on the surface of trabecule and in the osteocytes (brown staining). Mag. x 100. (Ob-osteoblasts, O-osteocytes, BM- bone marrow, T-trabeculae, F-fatty degeneration).

Article Snippet: SPARC antibody (H-90) rabbit polyclonal IgG and COL-1A antibody (COL-1) mouse monoclonal IgG were used for immunohistochemical study (IHH), according to the manufacturer's instructions (Santa Cruz USA and Scytek kits).

Techniques: Immunohistochemical staining, Expressing, Staining

Journal: Head & Face Medicine

Article Title: Behavior of osteoblastic cells cultured on titanium and structured zirconia surfaces

doi: 10.1186/1746-160X-4-29

Figure Lengend Snippet: Immunocytochemical analysis of characteristic bone derived proteins. After 7 days extracellular expression of collagen I and osteonectin is evident on all different surfaces examined. Scattered expression of osteocalcin is demonstrated (magnification 20-fold).

Article Snippet: After incubation for 7, 14, or 28 days at 37°C in an atmosphere of 5% CO 2 in the High GEM medium, primary antibodies were used according to the manufacturers' instructions: rabbit polyclonal anti-collagen I (Biotrend, Cologne, Germany), Mouse monoclonal anti-osteocalcin (TaKaRa Bio, MoBiTec, Goettingen, Germany) and rabbit polyclonal anti-osteonectin (SPARC; Chemicon Millipore GmbH, Schwalbach, Germany).

Techniques: Derivative Assay, Expressing

Journal: Head & Face Medicine

Article Title: Behavior of osteoblastic cells cultured on titanium and structured zirconia surfaces

doi: 10.1186/1746-160X-4-29

Figure Lengend Snippet: After 28 days expression of collagen I, osteocalcin and osteonectin is still evident on all different surfaces examined. Minimally denser accumulation of reticular collagen fibrils on zirconia surfaces as compared to titanium surfaces are observed (magnification 20-fold).

Article Snippet: After incubation for 7, 14, or 28 days at 37°C in an atmosphere of 5% CO 2 in the High GEM medium, primary antibodies were used according to the manufacturers' instructions: rabbit polyclonal anti-collagen I (Biotrend, Cologne, Germany), Mouse monoclonal anti-osteocalcin (TaKaRa Bio, MoBiTec, Goettingen, Germany) and rabbit polyclonal anti-osteonectin (SPARC; Chemicon Millipore GmbH, Schwalbach, Germany).

Techniques: Expressing

Journal: Toxins

Article Title: Target Mechanisms of the Cyanotoxin Cylindrospermopsin in Immortalized Human Airway Epithelial Cells

doi: 10.3390/toxins14110785

Figure Lengend Snippet: Western blot analyses of SPARC- (upper panel), claudin-6- (middle panel), and CEP55 expression in whole cell extracts of 16HBE14o − cells upon exposure for 0, 12, 24, or 36 h to cylindrospermopsin (1 µmol/L—yellow squares; 2.5 µmol/L—orange triangles; 5 µmol/L—red dots) or vehicle (0.1% methanol—green diamonds). Data are presented as ratios of densities of antibody-labeled bands of the respective proteins and those of β-actin (internal standard). Means ± S.D., n = 4–10 biological replicates. Significant differences inmeans compared with those of the respective vehicle controls: *— p < 0.05; **— p < 0.01; ***— p < 0.001.

Article Snippet: The rabbit polyclonal anti-SPARC antibody was obtained from Cell Signaling Technologies (Frankfurt/M., Germany).

Techniques: Western Blot, Expressing, Labeling