rabbit polyclonal anti sema3b  (Danaher Inc)

Journal: Medical Science Monitor

Article Title: Expression of Semaphorin 3B (SEMA3B) in Various Grades of Endometrial Cancer

doi: 10.12659/msm.916762

Figure Lengend Snippet: Figure 1. Immunohistochemical localization of SEMA3B in different grades of endometrial cancer. C – control; G – grade of endometrial cancer. 200× magnification.

Article Snippet: The analysis of SEMA3B level changes was performed based on the immunohistochemical reaction with rabbit polyclonal anti-SEMA3B antibody (Novus Biological).

Techniques: Immunohistochemical staining, Control

Journal: Biological Research

Article Title: Suppression of miR-221 inhibits glioma cells proliferation and invasion via targeting SEMA3B

doi: 10.1186/s40659-015-0030-y

Figure Lengend Snippet: SEMA3B is a target of miR-221. a Binding sites of miR-221 (the black letters ) on the SEMA3B mRNA 3′UTR. b An inverse correlation between miR-221 and SEMA3B was observed by Spearman correlation analysis in 30 cased of human glioma tissues. c Western blot was performed to detect the protein level of SEMA3B in NHA and U87MG cells with miR-221 overexpression or knockdown using miR-221 inhibitor or mimic (100 pM) for 48 h, respectively. d Luciferase reporter plasmids and miR-221 mimics were co-transfected to cells, and then the luciferase reporter assay was employed to detect luciferase activity. *p < 0.05; **p < 0.01.

Article Snippet: The rabbit polyclonal anti-SEMA3B antibody (code: sc-21204-R, 1:500 dilution) and the goat polyclonal anti-GAPDH antibody (code: sc-48167, 1:2,000 dilution) were both purchased from Santa Cruz Biotechnologies (CA, USA).

Techniques: Binding Assay, Western Blot, Over Expression, Knockdown, Luciferase, Transfection, Reporter Assay, Activity Assay

Journal: Biological Research

Article Title: Suppression of miR-221 inhibits glioma cells proliferation and invasion via targeting SEMA3B

doi: 10.1186/s40659-015-0030-y

Figure Lengend Snippet: Knockdown endogenous SEMA3B reversed the effect of miR-221 Inhibitor. a Western blot showed SEMA3B level in a U87MG cells with SEMA3B gene stably silenced, b CCK-8 assay was employed to detect the cell proliferation in U87MG wild type and SEMA3B KD cells treated with scramble or miR-221 inhibitor (100 pM). Transwell assay without matrigel coated for testing cell migration ( c ) and transwell assay with matrigel coated was employed to detect the cell invasion ability ( d ) in U87MG cells treated with scramble or miR-221 inhibitor (100 pM). *p < 0.05; **p < 0.01.

Article Snippet: The rabbit polyclonal anti-SEMA3B antibody (code: sc-21204-R, 1:500 dilution) and the goat polyclonal anti-GAPDH antibody (code: sc-48167, 1:2,000 dilution) were both purchased from Santa Cruz Biotechnologies (CA, USA).

Techniques: Knockdown, Western Blot, Stable Transfection, CCK-8 Assay, Transwell Assay, Migration