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Cell Signaling Technology Inc rabbit polyclonal anti rat parvalbumin igg
Rabbit Polyclonal Anti Rat Parvalbumin Igg, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal anti rat parvalbumin igg/product/Cell Signaling Technology Inc
Average 85 stars, based on 6 article reviews
Price from $9.99 to $1999.99
rabbit polyclonal anti rat parvalbumin igg - by Bioz Stars, 2020-09
85/100 stars

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Incubation:

Article Title: Ginkgo biloba extract (EGb 761) prevents the ischemic brain injury-induced decrease in parvalbumin expression
Article Snippet: .. Sections were blocked with 1% normal goat serum in PBS for 1 h and incubated with a rabbit polyclonal anti-rat parvalbumin IgG (diluted 1:200, Cell Signaling Technology, Beverly, MA, USA) at 4℃ for 15 h. After washing with PBS, sections were incubated with biotin-conjugated goat anti-rabbit IgG (1:200 in PBS) for 1 h, followed by incubation with an avidin-biotin-peroxidase complex for 1 h from a Vector ABC Elite kit (Vector Laboratories Inc., Burlingame, CA, USA), incubated with DAB solution with 0.03% hydrogen peroxidase. .. For the positive cell counting, three fields for cortical region were selected from right cerebral cortex.

Article Title: Ginkgo biloba extract (EGb 761) prevents the ischemic brain injury-induced decrease in parvalbumin expression
Article Snippet: .. After washing membranes with TBST, membranes were incubated with the following antibodies: a rabbit polyclonal anti-rat parvalbumin IgG (diluted 1:1000, Cell Signaling Technology, Beverly, MA, USA) and a mouse monoclonal anti-rat actin IgG (diluted 1:1000, Santa Cruz Biotechnology, Santa Cruz, CA, USA). .. The membrane was incubated with secondary antibody (1:5000, Pierce, Rockford, IL, USA) and the ECL Western blot analysis system (Amersham Pharmacia Biotech, Piscataway, NJ, USA) was used for detection according to the manufacturer's protocol.

Avidin-Biotin Assay:

Article Title: Ginkgo biloba extract (EGb 761) prevents the ischemic brain injury-induced decrease in parvalbumin expression
Article Snippet: .. Sections were blocked with 1% normal goat serum in PBS for 1 h and incubated with a rabbit polyclonal anti-rat parvalbumin IgG (diluted 1:200, Cell Signaling Technology, Beverly, MA, USA) at 4℃ for 15 h. After washing with PBS, sections were incubated with biotin-conjugated goat anti-rabbit IgG (1:200 in PBS) for 1 h, followed by incubation with an avidin-biotin-peroxidase complex for 1 h from a Vector ABC Elite kit (Vector Laboratories Inc., Burlingame, CA, USA), incubated with DAB solution with 0.03% hydrogen peroxidase. .. For the positive cell counting, three fields for cortical region were selected from right cerebral cortex.

Plasmid Preparation:

Article Title: Ginkgo biloba extract (EGb 761) prevents the ischemic brain injury-induced decrease in parvalbumin expression
Article Snippet: .. Sections were blocked with 1% normal goat serum in PBS for 1 h and incubated with a rabbit polyclonal anti-rat parvalbumin IgG (diluted 1:200, Cell Signaling Technology, Beverly, MA, USA) at 4℃ for 15 h. After washing with PBS, sections were incubated with biotin-conjugated goat anti-rabbit IgG (1:200 in PBS) for 1 h, followed by incubation with an avidin-biotin-peroxidase complex for 1 h from a Vector ABC Elite kit (Vector Laboratories Inc., Burlingame, CA, USA), incubated with DAB solution with 0.03% hydrogen peroxidase. .. For the positive cell counting, three fields for cortical region were selected from right cerebral cortex.

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    Cell Signaling Technology Inc rabbit polyclonal anti rat parvalbumin igg
    Rabbit Polyclonal Anti Rat Parvalbumin Igg, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti rat parvalbumin igg/product/Cell Signaling Technology Inc
    Average 85 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    rabbit polyclonal anti rat parvalbumin igg - by Bioz Stars, 2020-09
    85/100 stars
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