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rabbit polyclonal anti nsp 5a3a  (Novus Biologicals)


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    Novus Biologicals rabbit polyclonal anti nsp 5a3a
    Figure 1. Image of silver stained gel and protein bands identified by proteomic analysis. Lane 1. Immunoprecipitation of MCF-12A total lysates using anti-B23. Lane 2. Immunoprecipitation of MCF-12A total lysates using anti-NSP <t>5a3a.</t>
    Rabbit Polyclonal Anti Nsp 5a3a, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti nsp 5a3a/product/Novus Biologicals
    Average 90 stars, based on 2 article reviews
    rabbit polyclonal anti nsp 5a3a - by Bioz Stars, 2026-05
    90/100 stars

    Images

    1) Product Images from "NSP 5a3a's link to nuclear-cyto proteins B23 and hnRNP-L between normal and aberrant breast cell lines."

    Article Title: NSP 5a3a's link to nuclear-cyto proteins B23 and hnRNP-L between normal and aberrant breast cell lines.

    Journal: Cell cycle (Georgetown, Tex.)

    doi: 10.4161/cc.9.6.10991

    Figure 1. Image of silver stained gel and protein bands identified by proteomic analysis. Lane 1. Immunoprecipitation of MCF-12A total lysates using anti-B23. Lane 2. Immunoprecipitation of MCF-12A total lysates using anti-NSP 5a3a.
    Figure Legend Snippet: Figure 1. Image of silver stained gel and protein bands identified by proteomic analysis. Lane 1. Immunoprecipitation of MCF-12A total lysates using anti-B23. Lane 2. Immunoprecipitation of MCF-12A total lysates using anti-NSP 5a3a.

    Techniques Used: Staining, Immunoprecipitation

    Figure 3. Subcellular localization of NSP 5a3a in MCF-7 cells by immunostaining. MCF-7 cells were stained with rabbit anti-NSP5a3a then with anti- rabbit Tritc secondary. Subsequently, MCF-7 cells were stained with mouse anti-B23 then with anti-rabbit Fitc secondary to correlate how NSP 5a3a localizes with respect to B23. Controls consisted of MCF-12A cells stained with anti-rabbit Tritc secondary only and anti-rabbit Fitc secondary only. Nuc (nucleus): nuc (nucleoli).
    Figure Legend Snippet: Figure 3. Subcellular localization of NSP 5a3a in MCF-7 cells by immunostaining. MCF-7 cells were stained with rabbit anti-NSP5a3a then with anti- rabbit Tritc secondary. Subsequently, MCF-7 cells were stained with mouse anti-B23 then with anti-rabbit Fitc secondary to correlate how NSP 5a3a localizes with respect to B23. Controls consisted of MCF-12A cells stained with anti-rabbit Tritc secondary only and anti-rabbit Fitc secondary only. Nuc (nucleus): nuc (nucleoli).

    Techniques Used: Immunostaining, Staining

    Figure 2. Subcellular localization of NSP 5a3a in MCF-12A cells by immunostaining. MCF-12A cells were stained with rabbit anti-NSP5a3a then with anti-rabbit Tritc secondary. Subsequently, MCF-12a cells were stained with mouse anti-B23 then with anti-rabbit Fitc secondary to correlate how NSP 5a3a localizes with respect to B23. Controls consisted of MCF-12A cells stained with anti-rabbit Tritc secondary only and anti-rabbit Fitc secondary only. Nuc (nucleus): nuc (nucleoli).
    Figure Legend Snippet: Figure 2. Subcellular localization of NSP 5a3a in MCF-12A cells by immunostaining. MCF-12A cells were stained with rabbit anti-NSP5a3a then with anti-rabbit Tritc secondary. Subsequently, MCF-12a cells were stained with mouse anti-B23 then with anti-rabbit Fitc secondary to correlate how NSP 5a3a localizes with respect to B23. Controls consisted of MCF-12A cells stained with anti-rabbit Tritc secondary only and anti-rabbit Fitc secondary only. Nuc (nucleus): nuc (nucleoli).

    Techniques Used: Immunostaining, Staining

    Figure 4. Western blot analysis of Co-immunoprecipitations of MCF-7 and MCF-12A total cell lysates. (A) MCF-7 cell lysate immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-hnRNP-L. (B) MCF-7 cell lysate immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (C) MCF-7 cell lysate immunoprecipitation using anti-B23 and western blot analysis using anti-hnRNP-L. (D) MCF-7 cell lysate im- munoprecipitation using anti-hnRNP-L and western blot analysis using anti-B23. (E) MCF-7 cell lysate immunoprecipitation using anti-B23 and western blot analysis using anti-NSP 5a3a. (F) MCF-7 cell lysate immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-B23. (G) MCF-12A cell lysate immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-hnRNP-L. (H) MCF-7 cell lysate immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (I) MCF-7 cell lysate immunoprecipitation using anti-B23 and western blot analysis using anti-hnRNP-L. (J) MCF-7 cell lysate immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-B23. (K) MCF-7 cell lysate immuno- precipitation using anti-B23 and western blot analysis using anti-NSP 5a3a. (L) MCF-7 cell lysate immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-B23.
    Figure Legend Snippet: Figure 4. Western blot analysis of Co-immunoprecipitations of MCF-7 and MCF-12A total cell lysates. (A) MCF-7 cell lysate immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-hnRNP-L. (B) MCF-7 cell lysate immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (C) MCF-7 cell lysate immunoprecipitation using anti-B23 and western blot analysis using anti-hnRNP-L. (D) MCF-7 cell lysate im- munoprecipitation using anti-hnRNP-L and western blot analysis using anti-B23. (E) MCF-7 cell lysate immunoprecipitation using anti-B23 and western blot analysis using anti-NSP 5a3a. (F) MCF-7 cell lysate immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-B23. (G) MCF-12A cell lysate immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-hnRNP-L. (H) MCF-7 cell lysate immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (I) MCF-7 cell lysate immunoprecipitation using anti-B23 and western blot analysis using anti-hnRNP-L. (J) MCF-7 cell lysate immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-B23. (K) MCF-7 cell lysate immuno- precipitation using anti-B23 and western blot analysis using anti-NSP 5a3a. (L) MCF-7 cell lysate immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-B23.

    Techniques Used: Western Blot, Immunoprecipitation

    Figure 6. Western blot analysis of co-immunoprecipitations of MCF-7 cytoplasmic and nuclear fractions. (A) MCF-7 cytoplasmic immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-B23. (B) MCF-7 cytoplasmic immunoprecipitation using anti-B23 and western blot analy- sis using anti-hnRNP-L. (C) MCF-7 cytoplasmic immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-hnRNP-L. (D) MCF-7 cytoplasmic immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (E) MCF-7 nuclear immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-B23. (F) MCF-7 nuclear immunoprecipitation using anti-B23 and western blot analysis using anti- hnRNP-L. (G) MCF-7 nuclear immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-B23. (H) MCF-7 nuclear immunoprecipita- tion using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (I) MCF-7 nuclear immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (J) MCF-7 nuclear immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-hnRNP-L. (K) Cytoplas- mic marker alpha-tubulin. (L) Nuclear marker Lamin A/C.
    Figure Legend Snippet: Figure 6. Western blot analysis of co-immunoprecipitations of MCF-7 cytoplasmic and nuclear fractions. (A) MCF-7 cytoplasmic immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-B23. (B) MCF-7 cytoplasmic immunoprecipitation using anti-B23 and western blot analy- sis using anti-hnRNP-L. (C) MCF-7 cytoplasmic immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-hnRNP-L. (D) MCF-7 cytoplasmic immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (E) MCF-7 nuclear immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-B23. (F) MCF-7 nuclear immunoprecipitation using anti-B23 and western blot analysis using anti- hnRNP-L. (G) MCF-7 nuclear immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-B23. (H) MCF-7 nuclear immunoprecipita- tion using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (I) MCF-7 nuclear immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (J) MCF-7 nuclear immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-hnRNP-L. (K) Cytoplas- mic marker alpha-tubulin. (L) Nuclear marker Lamin A/C.

    Techniques Used: Western Blot, Immunoprecipitation, Marker

    Figure 5. Western blot analysis of co-immunoprecipitations of MCF-12A cytoplasmic and nuclear fractions. (A) MCF-12A cytoplasmic immunoprecipi- tation using anti-hnRNP-L and western blot analysis using anti-B23. (B) MCF-12A cytoplasmic immunoprecipitation using anti-B23 and western blot analysis using anti-hnRNP-L. (C) MCF-12A cytoplasmic immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-hnRNP-L. (D) MCF-12A cytoplasmic immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (E) MCF-12A cytoplasmic immunopre- cipitation using anti-B23 and western blot analysis using anti-NSP 5a3a. (F) MCF-12A cytoplasmic immunoprecipitation using anti-NSP 5a3a and west- ern blot analysis using anti-B23. (G) MCF-12A nuclear immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-B23. (H) MCF-12A nuclear immunoprecipitation using anti-B23 and western blot analysis using anti-hnRNP-L. (I) MCF-12A nuclear immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-B23. (J) MCF-12A nuclear immunoprecipitation using anti-B23 and western blot analysis using anti-NSP 5a3a. (K) Cytoplasmic marker alpha-tubulin. (L) Nuclear marker Lamin A/C.
    Figure Legend Snippet: Figure 5. Western blot analysis of co-immunoprecipitations of MCF-12A cytoplasmic and nuclear fractions. (A) MCF-12A cytoplasmic immunoprecipi- tation using anti-hnRNP-L and western blot analysis using anti-B23. (B) MCF-12A cytoplasmic immunoprecipitation using anti-B23 and western blot analysis using anti-hnRNP-L. (C) MCF-12A cytoplasmic immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-hnRNP-L. (D) MCF-12A cytoplasmic immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (E) MCF-12A cytoplasmic immunopre- cipitation using anti-B23 and western blot analysis using anti-NSP 5a3a. (F) MCF-12A cytoplasmic immunoprecipitation using anti-NSP 5a3a and west- ern blot analysis using anti-B23. (G) MCF-12A nuclear immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-B23. (H) MCF-12A nuclear immunoprecipitation using anti-B23 and western blot analysis using anti-hnRNP-L. (I) MCF-12A nuclear immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-B23. (J) MCF-12A nuclear immunoprecipitation using anti-B23 and western blot analysis using anti-NSP 5a3a. (K) Cytoplasmic marker alpha-tubulin. (L) Nuclear marker Lamin A/C.

    Techniques Used: Western Blot, Immunoprecipitation, Marker



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    rabbit polyclonal anti nsp 5a3a  (Novus Biologicals)
    90
    Novus Biologicals rabbit polyclonal anti nsp 5a3a
    Figure 1. Image of silver stained gel and protein bands identified by proteomic analysis. Lane 1. Immunoprecipitation of MCF-12A total lysates using anti-B23. Lane 2. Immunoprecipitation of MCF-12A total lysates using anti-NSP <t>5a3a.</t>
    Rabbit Polyclonal Anti Nsp 5a3a, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti nsp 5a3a/product/Novus Biologicals
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal anti nsp 5a3a - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier

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    Figure 1. Image of silver stained gel and protein bands identified by proteomic analysis. Lane 1. Immunoprecipitation of MCF-12A total lysates using anti-B23. Lane 2. Immunoprecipitation of MCF-12A total lysates using anti-NSP 5a3a.

    Journal: Cell cycle (Georgetown, Tex.)

    Article Title: NSP 5a3a's link to nuclear-cyto proteins B23 and hnRNP-L between normal and aberrant breast cell lines.

    doi: 10.4161/cc.9.6.10991

    Figure Lengend Snippet: Figure 1. Image of silver stained gel and protein bands identified by proteomic analysis. Lane 1. Immunoprecipitation of MCF-12A total lysates using anti-B23. Lane 2. Immunoprecipitation of MCF-12A total lysates using anti-NSP 5a3a.

    Article Snippet: Cells were pre-blocked for 1 hour at RT with 8% BSA then cells incubated with rabbit polyclonal anti-NSP 5a3a (Novus) at 1/500 dilution in 1% BSA over night at 4°C.

    Techniques: Staining, Immunoprecipitation

    Figure 3. Subcellular localization of NSP 5a3a in MCF-7 cells by immunostaining. MCF-7 cells were stained with rabbit anti-NSP5a3a then with anti- rabbit Tritc secondary. Subsequently, MCF-7 cells were stained with mouse anti-B23 then with anti-rabbit Fitc secondary to correlate how NSP 5a3a localizes with respect to B23. Controls consisted of MCF-12A cells stained with anti-rabbit Tritc secondary only and anti-rabbit Fitc secondary only. Nuc (nucleus): nuc (nucleoli).

    Journal: Cell cycle (Georgetown, Tex.)

    Article Title: NSP 5a3a's link to nuclear-cyto proteins B23 and hnRNP-L between normal and aberrant breast cell lines.

    doi: 10.4161/cc.9.6.10991

    Figure Lengend Snippet: Figure 3. Subcellular localization of NSP 5a3a in MCF-7 cells by immunostaining. MCF-7 cells were stained with rabbit anti-NSP5a3a then with anti- rabbit Tritc secondary. Subsequently, MCF-7 cells were stained with mouse anti-B23 then with anti-rabbit Fitc secondary to correlate how NSP 5a3a localizes with respect to B23. Controls consisted of MCF-12A cells stained with anti-rabbit Tritc secondary only and anti-rabbit Fitc secondary only. Nuc (nucleus): nuc (nucleoli).

    Article Snippet: Cells were pre-blocked for 1 hour at RT with 8% BSA then cells incubated with rabbit polyclonal anti-NSP 5a3a (Novus) at 1/500 dilution in 1% BSA over night at 4°C.

    Techniques: Immunostaining, Staining

    Figure 2. Subcellular localization of NSP 5a3a in MCF-12A cells by immunostaining. MCF-12A cells were stained with rabbit anti-NSP5a3a then with anti-rabbit Tritc secondary. Subsequently, MCF-12a cells were stained with mouse anti-B23 then with anti-rabbit Fitc secondary to correlate how NSP 5a3a localizes with respect to B23. Controls consisted of MCF-12A cells stained with anti-rabbit Tritc secondary only and anti-rabbit Fitc secondary only. Nuc (nucleus): nuc (nucleoli).

    Journal: Cell cycle (Georgetown, Tex.)

    Article Title: NSP 5a3a's link to nuclear-cyto proteins B23 and hnRNP-L between normal and aberrant breast cell lines.

    doi: 10.4161/cc.9.6.10991

    Figure Lengend Snippet: Figure 2. Subcellular localization of NSP 5a3a in MCF-12A cells by immunostaining. MCF-12A cells were stained with rabbit anti-NSP5a3a then with anti-rabbit Tritc secondary. Subsequently, MCF-12a cells were stained with mouse anti-B23 then with anti-rabbit Fitc secondary to correlate how NSP 5a3a localizes with respect to B23. Controls consisted of MCF-12A cells stained with anti-rabbit Tritc secondary only and anti-rabbit Fitc secondary only. Nuc (nucleus): nuc (nucleoli).

    Article Snippet: Cells were pre-blocked for 1 hour at RT with 8% BSA then cells incubated with rabbit polyclonal anti-NSP 5a3a (Novus) at 1/500 dilution in 1% BSA over night at 4°C.

    Techniques: Immunostaining, Staining

    Figure 4. Western blot analysis of Co-immunoprecipitations of MCF-7 and MCF-12A total cell lysates. (A) MCF-7 cell lysate immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-hnRNP-L. (B) MCF-7 cell lysate immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (C) MCF-7 cell lysate immunoprecipitation using anti-B23 and western blot analysis using anti-hnRNP-L. (D) MCF-7 cell lysate im- munoprecipitation using anti-hnRNP-L and western blot analysis using anti-B23. (E) MCF-7 cell lysate immunoprecipitation using anti-B23 and western blot analysis using anti-NSP 5a3a. (F) MCF-7 cell lysate immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-B23. (G) MCF-12A cell lysate immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-hnRNP-L. (H) MCF-7 cell lysate immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (I) MCF-7 cell lysate immunoprecipitation using anti-B23 and western blot analysis using anti-hnRNP-L. (J) MCF-7 cell lysate immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-B23. (K) MCF-7 cell lysate immuno- precipitation using anti-B23 and western blot analysis using anti-NSP 5a3a. (L) MCF-7 cell lysate immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-B23.

    Journal: Cell cycle (Georgetown, Tex.)

    Article Title: NSP 5a3a's link to nuclear-cyto proteins B23 and hnRNP-L between normal and aberrant breast cell lines.

    doi: 10.4161/cc.9.6.10991

    Figure Lengend Snippet: Figure 4. Western blot analysis of Co-immunoprecipitations of MCF-7 and MCF-12A total cell lysates. (A) MCF-7 cell lysate immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-hnRNP-L. (B) MCF-7 cell lysate immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (C) MCF-7 cell lysate immunoprecipitation using anti-B23 and western blot analysis using anti-hnRNP-L. (D) MCF-7 cell lysate im- munoprecipitation using anti-hnRNP-L and western blot analysis using anti-B23. (E) MCF-7 cell lysate immunoprecipitation using anti-B23 and western blot analysis using anti-NSP 5a3a. (F) MCF-7 cell lysate immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-B23. (G) MCF-12A cell lysate immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-hnRNP-L. (H) MCF-7 cell lysate immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (I) MCF-7 cell lysate immunoprecipitation using anti-B23 and western blot analysis using anti-hnRNP-L. (J) MCF-7 cell lysate immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-B23. (K) MCF-7 cell lysate immuno- precipitation using anti-B23 and western blot analysis using anti-NSP 5a3a. (L) MCF-7 cell lysate immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-B23.

    Article Snippet: Cells were pre-blocked for 1 hour at RT with 8% BSA then cells incubated with rabbit polyclonal anti-NSP 5a3a (Novus) at 1/500 dilution in 1% BSA over night at 4°C.

    Techniques: Western Blot, Immunoprecipitation

    Figure 6. Western blot analysis of co-immunoprecipitations of MCF-7 cytoplasmic and nuclear fractions. (A) MCF-7 cytoplasmic immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-B23. (B) MCF-7 cytoplasmic immunoprecipitation using anti-B23 and western blot analy- sis using anti-hnRNP-L. (C) MCF-7 cytoplasmic immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-hnRNP-L. (D) MCF-7 cytoplasmic immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (E) MCF-7 nuclear immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-B23. (F) MCF-7 nuclear immunoprecipitation using anti-B23 and western blot analysis using anti- hnRNP-L. (G) MCF-7 nuclear immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-B23. (H) MCF-7 nuclear immunoprecipita- tion using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (I) MCF-7 nuclear immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (J) MCF-7 nuclear immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-hnRNP-L. (K) Cytoplas- mic marker alpha-tubulin. (L) Nuclear marker Lamin A/C.

    Journal: Cell cycle (Georgetown, Tex.)

    Article Title: NSP 5a3a's link to nuclear-cyto proteins B23 and hnRNP-L between normal and aberrant breast cell lines.

    doi: 10.4161/cc.9.6.10991

    Figure Lengend Snippet: Figure 6. Western blot analysis of co-immunoprecipitations of MCF-7 cytoplasmic and nuclear fractions. (A) MCF-7 cytoplasmic immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-B23. (B) MCF-7 cytoplasmic immunoprecipitation using anti-B23 and western blot analy- sis using anti-hnRNP-L. (C) MCF-7 cytoplasmic immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-hnRNP-L. (D) MCF-7 cytoplasmic immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (E) MCF-7 nuclear immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-B23. (F) MCF-7 nuclear immunoprecipitation using anti-B23 and western blot analysis using anti- hnRNP-L. (G) MCF-7 nuclear immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-B23. (H) MCF-7 nuclear immunoprecipita- tion using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (I) MCF-7 nuclear immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (J) MCF-7 nuclear immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-hnRNP-L. (K) Cytoplas- mic marker alpha-tubulin. (L) Nuclear marker Lamin A/C.

    Article Snippet: Cells were pre-blocked for 1 hour at RT with 8% BSA then cells incubated with rabbit polyclonal anti-NSP 5a3a (Novus) at 1/500 dilution in 1% BSA over night at 4°C.

    Techniques: Western Blot, Immunoprecipitation, Marker

    Figure 5. Western blot analysis of co-immunoprecipitations of MCF-12A cytoplasmic and nuclear fractions. (A) MCF-12A cytoplasmic immunoprecipi- tation using anti-hnRNP-L and western blot analysis using anti-B23. (B) MCF-12A cytoplasmic immunoprecipitation using anti-B23 and western blot analysis using anti-hnRNP-L. (C) MCF-12A cytoplasmic immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-hnRNP-L. (D) MCF-12A cytoplasmic immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (E) MCF-12A cytoplasmic immunopre- cipitation using anti-B23 and western blot analysis using anti-NSP 5a3a. (F) MCF-12A cytoplasmic immunoprecipitation using anti-NSP 5a3a and west- ern blot analysis using anti-B23. (G) MCF-12A nuclear immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-B23. (H) MCF-12A nuclear immunoprecipitation using anti-B23 and western blot analysis using anti-hnRNP-L. (I) MCF-12A nuclear immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-B23. (J) MCF-12A nuclear immunoprecipitation using anti-B23 and western blot analysis using anti-NSP 5a3a. (K) Cytoplasmic marker alpha-tubulin. (L) Nuclear marker Lamin A/C.

    Journal: Cell cycle (Georgetown, Tex.)

    Article Title: NSP 5a3a's link to nuclear-cyto proteins B23 and hnRNP-L between normal and aberrant breast cell lines.

    doi: 10.4161/cc.9.6.10991

    Figure Lengend Snippet: Figure 5. Western blot analysis of co-immunoprecipitations of MCF-12A cytoplasmic and nuclear fractions. (A) MCF-12A cytoplasmic immunoprecipi- tation using anti-hnRNP-L and western blot analysis using anti-B23. (B) MCF-12A cytoplasmic immunoprecipitation using anti-B23 and western blot analysis using anti-hnRNP-L. (C) MCF-12A cytoplasmic immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-hnRNP-L. (D) MCF-12A cytoplasmic immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-NSP 5a3a. (E) MCF-12A cytoplasmic immunopre- cipitation using anti-B23 and western blot analysis using anti-NSP 5a3a. (F) MCF-12A cytoplasmic immunoprecipitation using anti-NSP 5a3a and west- ern blot analysis using anti-B23. (G) MCF-12A nuclear immunoprecipitation using anti-hnRNP-L and western blot analysis using anti-B23. (H) MCF-12A nuclear immunoprecipitation using anti-B23 and western blot analysis using anti-hnRNP-L. (I) MCF-12A nuclear immunoprecipitation using anti-NSP 5a3a and western blot analysis using anti-B23. (J) MCF-12A nuclear immunoprecipitation using anti-B23 and western blot analysis using anti-NSP 5a3a. (K) Cytoplasmic marker alpha-tubulin. (L) Nuclear marker Lamin A/C.

    Article Snippet: Cells were pre-blocked for 1 hour at RT with 8% BSA then cells incubated with rabbit polyclonal anti-NSP 5a3a (Novus) at 1/500 dilution in 1% BSA over night at 4°C.

    Techniques: Western Blot, Immunoprecipitation, Marker