rabbit polyclonal anti nrf2 antibody (Proteintech)
Structured Review

Rabbit Polyclonal Anti Nrf2 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal anti nrf2 antibody/product/Proteintech
Average 86 stars, based on 1 article reviews
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1) Product Images from "Hydrogen sulfide reduces oxidative stress in Huntington’s disease via Nrf2"
Article Title: Hydrogen sulfide reduces oxidative stress in Huntington’s disease via Nrf2
Journal: Neural Regeneration Research
doi: 10.4103/NRR.NRR-D-23-01051

Figure Legend Snippet: Reagents and instruments used in the study
Techniques Used: CCK-8 Assay, Saline, TUNEL Assay, DNA Extraction, Modification, Marker, Fluorescence, Microscopy, Imaging

Figure Legend Snippet: NaHS reduces oxidative stress by suppressing Nrf2. (A) Representative images of DCFHDA staining in PC12 cells treated with Quin for 24 hours. The Quin group exhibited greater DCFH-DA (green) fluorescence than the Sham group. The Quin + NaHS group exhibited a decrease in DCFHDA (green) fluorescence compared with the Quin group, while an increase was observed in the Quin + NaHS + ML385 group. Scale bar: 50 μm. (B) DHE staining of PC12 cells following 24-hour Quin stimulation. Stronger DHE (red) fluorescence was observed in the Quin group compared with the Sham group. DHE (red) fluorescence was decreased in the Quin + NaHS group and increased in the Quin + NaHS + ML385 group compared with the Quin group. Scale bar: 50 μm. (C) DCFH-DA fluorescence intensity in PC12 cells was quantified using a fluorescence microplate reader ( n = 5 per group). (D) DHE fluorescence intensity in PC12 cells was quantified using a fluorescence microplate reader ( n = 5 per group). (E) DCFHDA fluorescence intensity in the striatum was quantified using a fluorescence microplate reader ( n = 5 per group). (F) Western blot detection of Nrf2 and HO-1 expression levels in PC12 cells in each group. Quantification of Nrf2 and HO-1 expression levels ( n = 3 per group). (G) Western blot detection of Nrf2 and HO-1 expression levels in the striatum of mice in each group. Quantification of Nrf2 and HO-1 expression levels ( n = 3 per group). The values are reported as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance followed by Bonferroni post hoc test). DCFHDA: 2′,7′-Dichlorodihydrofluorescein diacetate; DHE: dihydroethidium; HO-1: heme oxygenase-1; Nrf2: nuclear factor erythroid 2-related factor 2; Quin: quinolinic acid.
Techniques Used: Staining, Fluorescence, Western Blot, Expressing

Figure Legend Snippet: NaHS regulates mitochondrial function in Quin-stimulated PC12 cells by suppressing Nrf2. (A) Mitochondria stained with MitoTracker (red) in Quin-stimulated PC12 cells treated with NaHS and ML385. The Quin group exhibited severe mitochondrial fragmentation and a decrease in mitochondrial fluorescence intensity compared with the Sham group, whereas the addition of NaHS resulted in an increase in mitochondrial fluorescence intensity. However, this effect was reversed by the addition of ML385. Scale bar: 10 μm. (B) Quantification of mitochondrial fluorescence intensity ( n = 10 per group). (C) Representative micrographs of MMP in Quin-stimulated PC12 cells, as detected via JC-1. The transition from green to red fluorescence indicates mitochondrial polarization, which was restored to normal levels by NaHS treatment. In contrast, ML385 treatment induced a shift from green to red fluorescence, indicating substantial mitochondrial depolarization. Scale bar: 50 μm. (D) Quantification of mitochondrial MMP ( n = 15 per group). (E) Quantification of mitochondrial DNA (mtDNA, NADH5) versus nuclear DNA (GAPDH) as detected by real-time polymerase chain reaction in Quin-stimulated PC12 cells treated with NaHS and ML385. (F) Western blot detection of TOMM20 expression levels in PC12 cells. Quantification of TOMM20 expression ( n = 3 per group). The values are reported as mean ± SD. ** P < 0.01, *** P < 0.001 (one-way analysis of variance followed by Bonferroni post hoc test). GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; MMP: mitochondrial membrane potential; Quin: quinolinic acid; TOMM20: translocase of outer mitochondrial membrane 20.
Techniques Used: Staining, Fluorescence, Real-time Polymerase Chain Reaction, Western Blot, Expressing, Membrane