rabbit polyclonal anti neurod1  (Proteintech)

Journal: BMC Neuroscience

Article Title: Sonic hedgehog (Shh)/Gli modulates the spatial organization of neuroepithelial cell proliferation in the developing chick optic tectum

doi: 10.1186/1471-2202-13-117

Figure Lengend Snippet: Radial organization and immunocytochemical patterns at the end of DS1. A . Ectopic expression of pShhiEGFP in an electroporated OT (ED4.5); patches of positive NE cell bodies at the ventricular zone (VZ) and of postmitotic neurons at the premigratory zone (PMZ) can be observed. B . Hematoxylin-Eosin staining. Arrowheads point to mNE cells located along ventricular zone; they show PH3 nuclear labeling ( C ) and Notch reactive cytoplasm ( D ). Arrows point to neurons in the premigratory zone; they display nuclear NeuroD reactivity ( E ) and βIIITub reactive perikarya ( F ). Bar: 10 μm.

Article Snippet: Rabbit polyclonal anti-neurod (NeuroD1) , Lifespan Biosciences , , , Synthetic peptide DDDQKPKRRGPKKKKM Conjugated to malemide-activated KLH Human NeuroD1 (aa 76–91) , 1:100.

Techniques: Expressing, Staining, Labeling

Journal: BMC Neuroscience

Article Title: Sonic hedgehog (Shh)/Gli modulates the spatial organization of neuroepithelial cell proliferation in the developing chick optic tectum

doi: 10.1186/1471-2202-13-117

Figure Lengend Snippet: Primary antibodies characteristics

Article Snippet: Rabbit polyclonal anti-neurod (NeuroD1) , Lifespan Biosciences , , , Synthetic peptide DDDQKPKRRGPKKKKM Conjugated to malemide-activated KLH Human NeuroD1 (aa 76–91) , 1:100.

Techniques: Translocation Assay, Recombinant

Journal: Molecular Medicine Reports

Article Title: Abnormal differentiation of stem cells into enteroendocrine cells in rats with DSS-induced colitis

doi: 10.3892/mmr.2017.6266

Figure Lengend Snippet: Densities of (A) Msi1-, (B) Math-1, (C) Neurog3- (D) and NeuroD1-immunoreactive cells in the control, DSS, DSS-G and DSS-Q groups. *P<0.001. DSS, dextran sulfate sodium-induced colitis vehicle-treated group; DSS-G, 3-[(dodecylthiocarbonyl)-methyl]-glutarimide-treated DSS group; DSS-Q, dehydroxymethylepoxyquinomicin-treated DSS group; Msi1, Musashi 1; Neurog3, neurogenin 3; NeuroD1, neurogenic differentiation D1.

Article Snippet: The sections were incubated with the following primary antibodies for 32 min at 37°C: Monoclonal mouse anti-N-terminal of purified chromogranin A (CgA; 1:1,500; cat. no. M869; Dako Denmark A/S, Glostrup, Denmark); polyclonal rabbit anti-residues 5–21 [APQPGLASPDSPHDPCK] of the human, mouse and rat Musashi 1 (Msi1) protein (1:100; cat. no. NB100-1759; R&D Systems Europe, Abingdon, UK); polyclonal rabbit anti-synthetic peptide surrounding amino acid 190 of human Math-1 (1:50; code no. 3658-100; BioVision, Inc., Milpitas, CA, USA); polyclonal rabbit anti-KLH-conjugated synthetic peptide between 40–69 amino acids from the N-terminal region of human Neurog3 (1:50; cat. no. PA5-11893, Thermo Fisher, Oslo, Norway); and polyclonal rabbit anti-recombinant full-length human neurogenic differentiation D1 (NeuroD1; 1:50; cat. no. PA5-47381; Thermo Fisher).

Techniques:

Journal:

Article Title: NeuroD1/E47 Regulates the E-box Element of a Novel Zinc Finger Transcription Factor, IA-1, in Developing Nervous System *

doi: 10.1074/jbc.M306795200

Figure Lengend Snippet: A, dose-dependent activation of the IA-1 promoter by NeuroD1 in HeLa cells. Different concentrations of CMV-NeuroD1 constructs were co-transfected with the IA-1 promoter-CAT construct into HeLa cells. Dosage-dependent activation of the IA-1 promoter by the CMV-NeuroD1 cDNA expression vector is shown as -fold increase. The CAT activities were normalized by protein concentrations. The transfections were repeated three times. B, co-transfection of E47 and/or NeuroD1 enhanced the IA-1 promoter activity in HeLa cells. E47 and NeuroD1 together demonstrated much higher promoter activity than each transcription factor alone, suggesting heterodimer activation of the IA-1 promoter. The CMV-β-gal vector was used as internal control to normalize transfection efficiency. The transfections were repeated three times.

Article Snippet: Synthesis of E47 and NeuroD1 was confirmed by Western blot analysis using an anti-E47 rabbit polyclonal antibody (Santa Cruz Biotechnology, Inc., Santa Cruz, CA) or an anti-NeuroD1 rabbit polyclonal antibody (CeMines).

Techniques: Activation Assay, Construct, Transfection, Expressing, Plasmid Preparation, Cotransfection, Activity Assay

Journal:

Article Title: NeuroD1/E47 Regulates the E-box Element of a Novel Zinc Finger Transcription Factor, IA-1, in Developing Nervous System *

doi: 10.1074/jbc.M306795200

Figure Lengend Snippet: Three copies of each E-box (E1, E2, and E3) were cloned into E1bTATA-CAT vector. Each reporter vector was co-transfected with pcDNA, NeuroD1, E47, or E47-NeuroD1 into HeLa cells. The E47 homodimer and the E47-NeuroD1 heterodimer showed a strong activation of the E3-box construct. The CMV-β-gal vector was used as internal control to normalize transfection efficiency. The transfections were repeated three times.

Article Snippet: Synthesis of E47 and NeuroD1 was confirmed by Western blot analysis using an anti-E47 rabbit polyclonal antibody (Santa Cruz Biotechnology, Inc., Santa Cruz, CA) or an anti-NeuroD1 rabbit polyclonal antibody (CeMines).

Techniques: Clone Assay, Plasmid Preparation, Transfection, Activation Assay, Construct

Journal:

Article Title: NeuroD1/E47 Regulates the E-box Element of a Novel Zinc Finger Transcription Factor, IA-1, in Developing Nervous System *

doi: 10.1074/jbc.M306795200

Figure Lengend Snippet: A, the NeuroD1/E47 heterodimer and the E47 homodimer can specifically bind to the E3-box. The radiolabeled IA-1 promoter (−192/−165 bp) containing the E3-box was incubated with E47 or E47-NeuroD1 with or without antibodies to E47 or NeuroD1. Both the homodimer and the heterodimer were shown in shifted bands. E47 antibody supershifted the homodimer, whereas the NeuroD1 antibody interfered with the heterodimer binding. NS, nonspecific band. B, competitive inhibition of the E47-NeuroD1 complex binding with 3× E3-box. Incubation of E47 and NeuroD1 proteins with labeled 3× E3 oligonucleotide in the presence or absence of competitor (50- or 100-fold molar excess) showed that E47-NeuroD1 binding is specific for the E3-box alone.

Article Snippet: Synthesis of E47 and NeuroD1 was confirmed by Western blot analysis using an anti-E47 rabbit polyclonal antibody (Santa Cruz Biotechnology, Inc., Santa Cruz, CA) or an anti-NeuroD1 rabbit polyclonal antibody (CeMines).

Techniques: Incubation, Binding Assay, Inhibition, Labeling

Journal:

Article Title: NeuroD1/E47 Regulates the E-box Element of a Novel Zinc Finger Transcription Factor, IA-1, in Developing Nervous System *

doi: 10.1074/jbc.M306795200

Figure Lengend Snippet: Twenty micrograms of total RNA from Daoy, D283MED, U87MG, WERI-Rb-1, Y79, and βTC-1 cell lines were resolved on a 1% agarose/formaldehyde gel and transferred to a nitrocellulose membrane. The samples were run as two separate sets on the same gel. The membrane was divided in half, and the left half was hybridized with a mixture of human and mouse IA-1 probes (A) and the right half was hybridized with a hamster NeuroD1/β2 probe (B). The ethidium bromide-stained gel was shown below the blot for comparison of loading differences. The IA-1 gene expression in the neuroendocrine cell lines overlaps with the NeuroD1 message completely.

Article Snippet: Synthesis of E47 and NeuroD1 was confirmed by Western blot analysis using an anti-E47 rabbit polyclonal antibody (Santa Cruz Biotechnology, Inc., Santa Cruz, CA) or an anti-NeuroD1 rabbit polyclonal antibody (CeMines).

Techniques: Staining, Expressing