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Proteintech rabbit polyclonal anti dao antibody
Reagents and instruments used in the study
Rabbit Polyclonal Anti Dao Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Hydrogen sulfide reduces oxidative stress in Huntington’s disease via Nrf2"

Article Title: Hydrogen sulfide reduces oxidative stress in Huntington’s disease via Nrf2

Journal: Neural Regeneration Research

doi: 10.4103/NRR.NRR-D-23-01051

Reagents and instruments used in the study
Figure Legend Snippet: Reagents and instruments used in the study

Techniques Used: CCK-8 Assay, Saline, TUNEL Assay, DNA Extraction, Modification, Marker, Fluorescence, Microscopy, Imaging



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Millipore rabbit polyclonal anti aoc1
<t>AOC1</t> and WT1 protein expression in embryonic kidneys and gonads. Serial cryosections of a mouse embryo (13.5 d.p.c.) were stained with antibodies against AOC1 (a) and WT1 (b), respectively. Normal rabbit serum (NRbS) was used as a negative control (c). K, kidney; T, testis; M, mesonephros; A, adrenal gland. Representative double immunolabeling of AOC1 and WT1 in a 16.5 d.p.c. embryonic rat kidney (d–g) is shown. AOC1 (red) and WT1 (green) proteins were visualized with Cy3- and Alexa Fluor 488-conjugated secondary antibodies, respectively. Co-localization of AOC1 and WT1 in cells of the developing glomeruli is indicated by the yellow fluorescence signal in the merged image (g). Note that AOC1 protein is also present in cells that do not contain WT1 (d and g).
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Image Search Results


Reagents and instruments used in the study

Journal: Neural Regeneration Research

Article Title: Hydrogen sulfide reduces oxidative stress in Huntington’s disease via Nrf2

doi: 10.4103/NRR.NRR-D-23-01051

Figure Lengend Snippet: Reagents and instruments used in the study

Article Snippet: Rabbit polyclonal anti-DAO antibody , Proteintech Group , 13273-1-AP (RRID: AB_ 2877932).

Techniques: CCK-8 Assay, Saline, TUNEL Assay, DNA Extraction, Modification, Marker, Fluorescence, Microscopy, Imaging

AOC1 and WT1 protein expression in embryonic kidneys and gonads. Serial cryosections of a mouse embryo (13.5 d.p.c.) were stained with antibodies against AOC1 (a) and WT1 (b), respectively. Normal rabbit serum (NRbS) was used as a negative control (c). K, kidney; T, testis; M, mesonephros; A, adrenal gland. Representative double immunolabeling of AOC1 and WT1 in a 16.5 d.p.c. embryonic rat kidney (d–g) is shown. AOC1 (red) and WT1 (green) proteins were visualized with Cy3- and Alexa Fluor 488-conjugated secondary antibodies, respectively. Co-localization of AOC1 and WT1 in cells of the developing glomeruli is indicated by the yellow fluorescence signal in the merged image (g). Note that AOC1 protein is also present in cells that do not contain WT1 (d and g).

Journal: The Journal of Biological Chemistry

Article Title: Amine Oxidase Copper-containing 1 ( AOC1 ) Is a Downstream Target Gene of the Wilms Tumor Protein, WT1, during Kidney Development

doi: 10.1074/jbc.M114.564336

Figure Lengend Snippet: AOC1 and WT1 protein expression in embryonic kidneys and gonads. Serial cryosections of a mouse embryo (13.5 d.p.c.) were stained with antibodies against AOC1 (a) and WT1 (b), respectively. Normal rabbit serum (NRbS) was used as a negative control (c). K, kidney; T, testis; M, mesonephros; A, adrenal gland. Representative double immunolabeling of AOC1 and WT1 in a 16.5 d.p.c. embryonic rat kidney (d–g) is shown. AOC1 (red) and WT1 (green) proteins were visualized with Cy3- and Alexa Fluor 488-conjugated secondary antibodies, respectively. Co-localization of AOC1 and WT1 in cells of the developing glomeruli is indicated by the yellow fluorescence signal in the merged image (g). Note that AOC1 protein is also present in cells that do not contain WT1 (d and g).

Article Snippet: Single immunostainings for WT1 and AOC1 were performed on mouse tissues using the following primary antibodies diluted in ready-to-use antibody diluent (Zymed Laboratories Inc.): rabbit polyclonal anti-AOC1 (diluted 1:300; catalogue number AV41908, Sigma-Aldrich), rabbit polyclonal anti-WT1 (diluted 1:75; catalogue number sc-846, Santa Cruz Biotechnology, Inc.).

Techniques: Expressing, Staining, Negative Control, Immunolabeling, Fluorescence

Aoc1 and Odc1 mRNA in cultured murine embryonic kidneys. Both kidneys were isolated from murine embryos at 11.5, 12.5, and 13.5 d.p.c. and cultured for 72 h in the presence of either Wt1 antisense (Wt1 morpholino) or mismatch vivo-morpholino (mismatch). Aoc1 and Odc1 transcripts were measured by real time RT-PCR and normalized to Gapdh mRNA. A pairwise comparison was performed between kidneys incubated with Wt1 morpholino and mismatch morpholino. Values of the mismatch morpholino-treated kidneys were set to 1. Significant differences in Aoc1 (A) and Odc1 (B) mRNA levels are indicated (*, p < 0.05; **, p < 0.005; Student's paired t test). Knockdown efficiencies were assessed by immunoblotting with anti-WT1 antibody using actin as a loading control (C). n.s., not significant. D, analysis of ureter branching in kidneys (11.5, 12.5, and 13.5 d.p.c.) treated with either Wt1 or mismatch vivo-morpholino (10 μm each). After 48 h of culture in the presence of vivo-morpholinos, the whole mount preparations were stained with a FITC-conjugated anti-pancytokeratin antibody (dark field images), and a skeleton of the ureter was extracted manually (schematic images). Branching of the ureter was analyzed from the schematic images. Data are presented as means ± S.D. (error bars). * (p < 0.05) and ** (p < 0.005) indicate significant differences (Student's paired t test).

Journal: The Journal of Biological Chemistry

Article Title: Amine Oxidase Copper-containing 1 ( AOC1 ) Is a Downstream Target Gene of the Wilms Tumor Protein, WT1, during Kidney Development

doi: 10.1074/jbc.M114.564336

Figure Lengend Snippet: Aoc1 and Odc1 mRNA in cultured murine embryonic kidneys. Both kidneys were isolated from murine embryos at 11.5, 12.5, and 13.5 d.p.c. and cultured for 72 h in the presence of either Wt1 antisense (Wt1 morpholino) or mismatch vivo-morpholino (mismatch). Aoc1 and Odc1 transcripts were measured by real time RT-PCR and normalized to Gapdh mRNA. A pairwise comparison was performed between kidneys incubated with Wt1 morpholino and mismatch morpholino. Values of the mismatch morpholino-treated kidneys were set to 1. Significant differences in Aoc1 (A) and Odc1 (B) mRNA levels are indicated (*, p < 0.05; **, p < 0.005; Student's paired t test). Knockdown efficiencies were assessed by immunoblotting with anti-WT1 antibody using actin as a loading control (C). n.s., not significant. D, analysis of ureter branching in kidneys (11.5, 12.5, and 13.5 d.p.c.) treated with either Wt1 or mismatch vivo-morpholino (10 μm each). After 48 h of culture in the presence of vivo-morpholinos, the whole mount preparations were stained with a FITC-conjugated anti-pancytokeratin antibody (dark field images), and a skeleton of the ureter was extracted manually (schematic images). Branching of the ureter was analyzed from the schematic images. Data are presented as means ± S.D. (error bars). * (p < 0.05) and ** (p < 0.005) indicate significant differences (Student's paired t test).

Article Snippet: Single immunostainings for WT1 and AOC1 were performed on mouse tissues using the following primary antibodies diluted in ready-to-use antibody diluent (Zymed Laboratories Inc.): rabbit polyclonal anti-AOC1 (diluted 1:300; catalogue number AV41908, Sigma-Aldrich), rabbit polyclonal anti-WT1 (diluted 1:75; catalogue number sc-846, Santa Cruz Biotechnology, Inc.).

Techniques: Cell Culture, Isolation, Quantitative RT-PCR, Incubation, Western Blot, Staining

Aoc1 mRNA in cultured murine embryonic testis and ovary. The gonads were isolated from male (A) and female (B) murine embryos at the indicated developmental stages and cultured for 72 h in the presence of either Wt1 antisense (Wt1 morpholino) or mismatch vivo-morpholino (mismatch). Aoc1 transcripts were quantified by real time RT-PCR and normalized to Gapdh mRNA. A pairwise comparison was performed between Wt1 morpholino-treated gonads and the mismatch morpholino controls. Significant differences are indicated (*, p < 0.05; **, p < 0.005; Student's paired t test). Representative WT1 immunoblots to assess knockdown efficiencies are shown below the bar graphs.

Journal: The Journal of Biological Chemistry

Article Title: Amine Oxidase Copper-containing 1 ( AOC1 ) Is a Downstream Target Gene of the Wilms Tumor Protein, WT1, during Kidney Development

doi: 10.1074/jbc.M114.564336

Figure Lengend Snippet: Aoc1 mRNA in cultured murine embryonic testis and ovary. The gonads were isolated from male (A) and female (B) murine embryos at the indicated developmental stages and cultured for 72 h in the presence of either Wt1 antisense (Wt1 morpholino) or mismatch vivo-morpholino (mismatch). Aoc1 transcripts were quantified by real time RT-PCR and normalized to Gapdh mRNA. A pairwise comparison was performed between Wt1 morpholino-treated gonads and the mismatch morpholino controls. Significant differences are indicated (*, p < 0.05; **, p < 0.005; Student's paired t test). Representative WT1 immunoblots to assess knockdown efficiencies are shown below the bar graphs.

Article Snippet: Single immunostainings for WT1 and AOC1 were performed on mouse tissues using the following primary antibodies diluted in ready-to-use antibody diluent (Zymed Laboratories Inc.): rabbit polyclonal anti-AOC1 (diluted 1:300; catalogue number AV41908, Sigma-Aldrich), rabbit polyclonal anti-WT1 (diluted 1:75; catalogue number sc-846, Santa Cruz Biotechnology, Inc.).

Techniques: Cell Culture, Isolation, Quantitative RT-PCR, Western Blot

Aoc1 transcripts and WT1 protein in permanent cell lines. A, murine mesonephros-derived M15 cells were transfected either with a pool of four different siRNAs targeting the Wt1 gene (siWt1) or with non-targeting siRNAs (sicontrol). Aoc1 and Gapdh transcripts in siRNA-transfected M15 cells were quantified by real time RT-PCR. Down-regulation of WT1 protein in response to RNA interference was assessed by immunoblot analysis using anti-WT1 antibody. Detection of actin protein served as a loading control (D). B and C, Aoc1 mRNA in osteosarcoma-derived UB27 and UD28 cells, which express the WT1(−KTS) and WT1(+KTS) isoforms, respectively. AOC1 and GAPDH transcripts were measured by real time RT-PCR. Temporal changes of WT1 protein in response to tetracycline depletion were detected by immunoblot analysis. Values are shown as means ± S.D. (M15 cells, n = 5; UB27 cells, n = 4; UD28 cells, n = 3). Statistical significance is indicated by an asterisk (t test, p < 0.05) or hash tag (analysis of variance, F(3,10) = 8.195, p < 0.05).

Journal: The Journal of Biological Chemistry

Article Title: Amine Oxidase Copper-containing 1 ( AOC1 ) Is a Downstream Target Gene of the Wilms Tumor Protein, WT1, during Kidney Development

doi: 10.1074/jbc.M114.564336

Figure Lengend Snippet: Aoc1 transcripts and WT1 protein in permanent cell lines. A, murine mesonephros-derived M15 cells were transfected either with a pool of four different siRNAs targeting the Wt1 gene (siWt1) or with non-targeting siRNAs (sicontrol). Aoc1 and Gapdh transcripts in siRNA-transfected M15 cells were quantified by real time RT-PCR. Down-regulation of WT1 protein in response to RNA interference was assessed by immunoblot analysis using anti-WT1 antibody. Detection of actin protein served as a loading control (D). B and C, Aoc1 mRNA in osteosarcoma-derived UB27 and UD28 cells, which express the WT1(−KTS) and WT1(+KTS) isoforms, respectively. AOC1 and GAPDH transcripts were measured by real time RT-PCR. Temporal changes of WT1 protein in response to tetracycline depletion were detected by immunoblot analysis. Values are shown as means ± S.D. (M15 cells, n = 5; UB27 cells, n = 4; UD28 cells, n = 3). Statistical significance is indicated by an asterisk (t test, p < 0.05) or hash tag (analysis of variance, F(3,10) = 8.195, p < 0.05).

Article Snippet: Single immunostainings for WT1 and AOC1 were performed on mouse tissues using the following primary antibodies diluted in ready-to-use antibody diluent (Zymed Laboratories Inc.): rabbit polyclonal anti-AOC1 (diluted 1:300; catalogue number AV41908, Sigma-Aldrich), rabbit polyclonal anti-WT1 (diluted 1:75; catalogue number sc-846, Santa Cruz Biotechnology, Inc.).

Techniques: Derivative Assay, Transfection, Quantitative RT-PCR, Western Blot

WT1(−KTS) activates the promoter of the AOC1 gene. A, HEK293 cells were transiently transfected with reporter constructs expressing a firefly luciferase (Lucif.) under control of AOC1 promoter pieces of various lengths. The reporter constructs were co-transfected with either a WT1(−KTS) expression plasmid or empty vector. A Renilla luciferase plasmid was utilized for normalization of transfection efficiencies. Data shown are means ± S.E. (error bars) (n = 4). Statistical significance versus transfection with empty expression vector is indicated by an asterisk (p < 0.05, t test). mut., mutation; B, binding of WT1(−KTS) to the predicted element (lane 2 versus lane 1) in the human AOC1 promoter was proven by electrophoretic mobility shift assay. Interaction of WT1(−KTS) protein with the AOC1 promoter oligonucleotide could be competed with excess amounts of unlabeled DNA (lanes 3–5) containing the previously identified WT1 binding element of the murine Adamts16 promoter (43). Mutation of the WT1 consensus motif abrogated WT1(−KTS) binding (lanes 6 and 7). Interaction of WT1(−KTS) protein with the Adamts16 promoter element (43) served as an internal quality control (lanes 8 and 9). C, ChIP was performed to detect WT1 protein bound to the promoter region of the murine Aoc1 gene in its native chromosomal configuration. A specific antibody against WT1 was used for immunoprecipitation of chromatin prepared from M15 cells. Incubation with normal rabbit IgG (NRb-IgG) served as a negative control. Amplicons encompassing the 5′-flanking region of the Aoc1 gene were generated by real time PCR. The gene encoding anti-Müllerian hormone receptor 2 (Amhr2), a previously identified WT1 target (52), was used as a positive control. Data shown are means ± S.D. (error bars). Statistical significances between normal rabbit IgG and anti-WT1 are indicated by an asterisk (p < 0.05, paired t test, n = 7).

Journal: The Journal of Biological Chemistry

Article Title: Amine Oxidase Copper-containing 1 ( AOC1 ) Is a Downstream Target Gene of the Wilms Tumor Protein, WT1, during Kidney Development

doi: 10.1074/jbc.M114.564336

Figure Lengend Snippet: WT1(−KTS) activates the promoter of the AOC1 gene. A, HEK293 cells were transiently transfected with reporter constructs expressing a firefly luciferase (Lucif.) under control of AOC1 promoter pieces of various lengths. The reporter constructs were co-transfected with either a WT1(−KTS) expression plasmid or empty vector. A Renilla luciferase plasmid was utilized for normalization of transfection efficiencies. Data shown are means ± S.E. (error bars) (n = 4). Statistical significance versus transfection with empty expression vector is indicated by an asterisk (p < 0.05, t test). mut., mutation; B, binding of WT1(−KTS) to the predicted element (lane 2 versus lane 1) in the human AOC1 promoter was proven by electrophoretic mobility shift assay. Interaction of WT1(−KTS) protein with the AOC1 promoter oligonucleotide could be competed with excess amounts of unlabeled DNA (lanes 3–5) containing the previously identified WT1 binding element of the murine Adamts16 promoter (43). Mutation of the WT1 consensus motif abrogated WT1(−KTS) binding (lanes 6 and 7). Interaction of WT1(−KTS) protein with the Adamts16 promoter element (43) served as an internal quality control (lanes 8 and 9). C, ChIP was performed to detect WT1 protein bound to the promoter region of the murine Aoc1 gene in its native chromosomal configuration. A specific antibody against WT1 was used for immunoprecipitation of chromatin prepared from M15 cells. Incubation with normal rabbit IgG (NRb-IgG) served as a negative control. Amplicons encompassing the 5′-flanking region of the Aoc1 gene were generated by real time PCR. The gene encoding anti-Müllerian hormone receptor 2 (Amhr2), a previously identified WT1 target (52), was used as a positive control. Data shown are means ± S.D. (error bars). Statistical significances between normal rabbit IgG and anti-WT1 are indicated by an asterisk (p < 0.05, paired t test, n = 7).

Article Snippet: Single immunostainings for WT1 and AOC1 were performed on mouse tissues using the following primary antibodies diluted in ready-to-use antibody diluent (Zymed Laboratories Inc.): rabbit polyclonal anti-AOC1 (diluted 1:300; catalogue number AV41908, Sigma-Aldrich), rabbit polyclonal anti-WT1 (diluted 1:75; catalogue number sc-846, Santa Cruz Biotechnology, Inc.).

Techniques: Transfection, Construct, Expressing, Luciferase, Plasmid Preparation, Mutagenesis, Binding Assay, Electrophoretic Mobility Shift Assay, Immunoprecipitation, Incubation, Negative Control, Generated, Real-time Polymerase Chain Reaction, Positive Control

Effect of AOC1, given either alone or in combination with putrescine and vitamin C, respectively, on branching morphogenesis in embryonic kidney explants. Kidneys were excised from murine embryos at the indicated gestational stages and incubated for 48 h in the presence of AOC1 (50 milliunits/ml) alone (A) or together with 100 μm putrescine (B) and 200 μm vitamin C (VitC) (C), respectively. As a control, the contralateral kidney of each embryo was treated with BSA. After 48 h of culture, the ureter was stained with a FITC-conjugated anti-pancytokeratin antibody (dark field images), and a skeleton of the ureteric bud branches was drawn manually (schematic images). Branching of the ureter was analyzed based on the schematic sketches. The absolute numbers of branches are indicated in the columns. Data are presented as means ± S.D. (error bars). * (p < 0.05) and ** (p < 0.005) indicate significant differences (paired t test).

Journal: The Journal of Biological Chemistry

Article Title: Amine Oxidase Copper-containing 1 ( AOC1 ) Is a Downstream Target Gene of the Wilms Tumor Protein, WT1, during Kidney Development

doi: 10.1074/jbc.M114.564336

Figure Lengend Snippet: Effect of AOC1, given either alone or in combination with putrescine and vitamin C, respectively, on branching morphogenesis in embryonic kidney explants. Kidneys were excised from murine embryos at the indicated gestational stages and incubated for 48 h in the presence of AOC1 (50 milliunits/ml) alone (A) or together with 100 μm putrescine (B) and 200 μm vitamin C (VitC) (C), respectively. As a control, the contralateral kidney of each embryo was treated with BSA. After 48 h of culture, the ureter was stained with a FITC-conjugated anti-pancytokeratin antibody (dark field images), and a skeleton of the ureteric bud branches was drawn manually (schematic images). Branching of the ureter was analyzed based on the schematic sketches. The absolute numbers of branches are indicated in the columns. Data are presented as means ± S.D. (error bars). * (p < 0.05) and ** (p < 0.005) indicate significant differences (paired t test).

Article Snippet: Single immunostainings for WT1 and AOC1 were performed on mouse tissues using the following primary antibodies diluted in ready-to-use antibody diluent (Zymed Laboratories Inc.): rabbit polyclonal anti-AOC1 (diluted 1:300; catalogue number AV41908, Sigma-Aldrich), rabbit polyclonal anti-WT1 (diluted 1:75; catalogue number sc-846, Santa Cruz Biotechnology, Inc.).

Techniques: Incubation, Staining

Effect of putrescine and AOC1 inhibition by aminoguanidine on branching morphogenesis in embryonic kidney explants. Embryonic kidney organ cultures (11.5, 12.5, and 13.5 d.p.c.) were treated for 48 h with 100 μm putrescine (A) or 1 μm aminoguanidine to inhibit AOC1 enzyme activity (B). The contralateral kidney of each embryo was cultured in the presence of BSA. The preparations were processed and analyzed as described in the legend of Fig. 6. The absolute numbers of branches are given in the columns. Data are means ± S.D. (error bars). * (p < 0.05) and ** (p < 0.005) indicate significant differences (paired t test).

Journal: The Journal of Biological Chemistry

Article Title: Amine Oxidase Copper-containing 1 ( AOC1 ) Is a Downstream Target Gene of the Wilms Tumor Protein, WT1, during Kidney Development

doi: 10.1074/jbc.M114.564336

Figure Lengend Snippet: Effect of putrescine and AOC1 inhibition by aminoguanidine on branching morphogenesis in embryonic kidney explants. Embryonic kidney organ cultures (11.5, 12.5, and 13.5 d.p.c.) were treated for 48 h with 100 μm putrescine (A) or 1 μm aminoguanidine to inhibit AOC1 enzyme activity (B). The contralateral kidney of each embryo was cultured in the presence of BSA. The preparations were processed and analyzed as described in the legend of Fig. 6. The absolute numbers of branches are given in the columns. Data are means ± S.D. (error bars). * (p < 0.05) and ** (p < 0.005) indicate significant differences (paired t test).

Article Snippet: Single immunostainings for WT1 and AOC1 were performed on mouse tissues using the following primary antibodies diluted in ready-to-use antibody diluent (Zymed Laboratories Inc.): rabbit polyclonal anti-AOC1 (diluted 1:300; catalogue number AV41908, Sigma-Aldrich), rabbit polyclonal anti-WT1 (diluted 1:75; catalogue number sc-846, Santa Cruz Biotechnology, Inc.).

Techniques: Inhibition, Activity Assay, Cell Culture