Journal: Infectious Agents and Cancer
Article Title: A systematic study on the treatment of hepatitis B-related hepatocellular carcinoma with drugs based on bioinformatics and key target reverse network pharmacology and experimental verification
doi: 10.1186/s13027-023-00520-z
Figure Lengend Snippet: Abnormal expression of 6 key genes in HBV-related HCC. A. 6 key genes were more highly expressed in liver cancer tissues compared with those in the normal tissues in GEPIA database. The red and gray boxes represent cancer and normal tissues, respectively. B. The stage of (a) AURKA, (b) BIRC5, (c) CCNB1, (d) CDK1, (e) CDKN3 and (f) TYMS using UALCAN database. C. The immunohistochemical images of (a) AURKA, (b) BIRC5, (c) CCNB1, (d) CDK1, (e) TYMS in liver and HCC tissues using the HPA database
Article Snippet: The primary antibodies were rabbit anti-CDK1 polyclonal antibody (Cat. # CY5061, Abways, diluted to 1:2000), anti-CCNB1 polyclonal antibody (Cat. # CY5378, Abways, diluted to 1:2000), and β-actin antibody (Cat. # AC026, abclonal, diluted to 1:50,000).
Techniques: Expressing, Immunohistochemical staining