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Altered receptor expression in mediating diminished BSM contractility upon pressure treatment. A, Western blot images of M2, M3, and <t>P2X1</t> receptor protein expression in mouse bladders from control (n = 4) and pressure treated mice (50 cm: n = 4 for 0 hours, 6 hours, and 24 hours, respectively. 80 cm, n = 4 for 0 hours, 6 hours, and 24 hours, respectively). B-D, are quantitated data by densitometry and normalized to actin. Data are shown as box and whiskers, center line is the median of the data set, box represents 75% of the data, and bars indicate whiskers from minimum to maximum. Data were analyzed by Student’s t test between control group and 50 or 80 cm H2O pressure treated groups. *P < .05, **P < .001
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P2 receptors (P2X 1 , P2X 4 , P2X 7 , P2Y 1 , and P2Y 11 ) compared by Western blotting in B cells and LCLs . Proteins extracted from B cells and LCLs were probed with rabbit <t>polyclonal</t> antibodies directed against P2X 1 (60-kDa), P2X 4 (65-kDa), P2X 7 (68-kDa), P2Y 1 (66-kDa), and P2Y 11 (50-kDa) (left). Membranes were re-probed by GAPDH (40-kDa) (right). Data are representatives of 4 independent experiments.
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Alomone Labs rabbit anti p2x1 6
Expression and size distribution of <t>P2X1-positive</t> cells to total and FG-positive DRG neurons between the control and CCI groups. (a) P2X1-positive fluorescence images of total and FG-positive DRG between the control and CCI groups (200x, scale is 50 μ m). (b) The percentages of the expression and size distribution of P2X1-positive cells to total and FG-positive DRG neurons between the control and CCI groups. There was no significant difference between the control and CCI groups, n = 6.
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Altered receptor expression in mediating diminished BSM contractility upon pressure treatment. A, Western blot images of M2, M3, and P2X1 receptor protein expression in mouse bladders from control (n = 4) and pressure treated mice (50 cm: n = 4 for 0 hours, 6 hours, and 24 hours, respectively. 80 cm, n = 4 for 0 hours, 6 hours, and 24 hours, respectively). B-D, are quantitated data by densitometry and normalized to actin. Data are shown as box and whiskers, center line is the median of the data set, box represents 75% of the data, and bars indicate whiskers from minimum to maximum. Data were analyzed by Student’s t test between control group and 50 or 80 cm H2O pressure treated groups. *P < .05, **P < .001

Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Molecular mechanisms of voiding dysfunction in a novel mouse model of acute urinary retention

doi: 10.1096/fj.202002415R

Figure Lengend Snippet: Altered receptor expression in mediating diminished BSM contractility upon pressure treatment. A, Western blot images of M2, M3, and P2X1 receptor protein expression in mouse bladders from control (n = 4) and pressure treated mice (50 cm: n = 4 for 0 hours, 6 hours, and 24 hours, respectively. 80 cm, n = 4 for 0 hours, 6 hours, and 24 hours, respectively). B-D, are quantitated data by densitometry and normalized to actin. Data are shown as box and whiskers, center line is the median of the data set, box represents 75% of the data, and bars indicate whiskers from minimum to maximum. Data were analyzed by Student’s t test between control group and 50 or 80 cm H2O pressure treated groups. *P < .05, **P < .001

Article Snippet: Western blot analysis Rabbit anti-P2X1 antibody (1:1000, #APR-001) and rabbit anti-chrm2 antibody (1:1000, #AMR-002) were purchased from Alomone Lab. Rabbit anti-chrm3 antibody (1:1000, #PA5–77485) was purchased from Thermo Fisher Scientific.

Techniques: Expressing, Western Blot, Control

P2 receptors (P2X 1 , P2X 4 , P2X 7 , P2Y 1 , and P2Y 11 ) compared by Western blotting in B cells and LCLs . Proteins extracted from B cells and LCLs were probed with rabbit polyclonal antibodies directed against P2X 1 (60-kDa), P2X 4 (65-kDa), P2X 7 (68-kDa), P2Y 1 (66-kDa), and P2Y 11 (50-kDa) (left). Membranes were re-probed by GAPDH (40-kDa) (right). Data are representatives of 4 independent experiments.

Journal: BMC Immunology

Article Title: Expression of P2 receptors in human B cells and Epstein-Barr virus-transformed lymphoblastoid cell lines

doi: 10.1186/1471-2172-7-22

Figure Lengend Snippet: P2 receptors (P2X 1 , P2X 4 , P2X 7 , P2Y 1 , and P2Y 11 ) compared by Western blotting in B cells and LCLs . Proteins extracted from B cells and LCLs were probed with rabbit polyclonal antibodies directed against P2X 1 (60-kDa), P2X 4 (65-kDa), P2X 7 (68-kDa), P2Y 1 (66-kDa), and P2Y 11 (50-kDa) (left). Membranes were re-probed by GAPDH (40-kDa) (right). Data are representatives of 4 independent experiments.

Article Snippet: The membrane was incubated with rabbit polyclonal antibodies (Alomone Labs, Jerusalem, Israel) against P2X 1 receptor, P2X 4 receptor, P2X 7 receptor, P2Y 1 receptor, or P2Y 11 receptor in TBST for 2 hours at room temperature, washed with TBST, and incubated with secondary anti-rabbit IgG (Amersham Pharmacia Biotech) in TBST for 1 hour.

Techniques: Western Blot

Expression and size distribution of P2X1-positive cells to total and FG-positive DRG neurons between the control and CCI groups. (a) P2X1-positive fluorescence images of total and FG-positive DRG between the control and CCI groups (200x, scale is 50 μ m). (b) The percentages of the expression and size distribution of P2X1-positive cells to total and FG-positive DRG neurons between the control and CCI groups. There was no significant difference between the control and CCI groups, n = 6.

Journal: BioMed Research International

Article Title: Retrograde Labeling of Different Distribution Features of DRG P2X2 and P2X3 Receptors in a Neuropathic Pain Rat Model

doi: 10.1155/2020/9861459

Figure Lengend Snippet: Expression and size distribution of P2X1-positive cells to total and FG-positive DRG neurons between the control and CCI groups. (a) P2X1-positive fluorescence images of total and FG-positive DRG between the control and CCI groups (200x, scale is 50 μ m). (b) The percentages of the expression and size distribution of P2X1-positive cells to total and FG-positive DRG neurons between the control and CCI groups. There was no significant difference between the control and CCI groups, n = 6.

Article Snippet: The primary antibodies were rabbit anti-P2X1-6 (RRID/Cat: AB_2341048, #APR-022; AB_2341051, #APR-025; AB_2341052, #APR-026; AB_2341050, #APR-024; AB_2756757, #APR-027; and AB_2756758, #APR-028, respectively, diluted 1 : 200 with 0.1 M PBS, Alomone Labs, Israel).

Techniques: Expressing, Fluorescence