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Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Article Title: Molecular mechanisms of voiding dysfunction in a novel mouse model of acute urinary retention
doi: 10.1096/fj.202002415R
Figure Lengend Snippet: Altered receptor expression in mediating diminished BSM contractility upon pressure treatment. A, Western blot images of M2, M3, and P2X1 receptor protein expression in mouse bladders from control (n = 4) and pressure treated mice (50 cm: n = 4 for 0 hours, 6 hours, and 24 hours, respectively. 80 cm, n = 4 for 0 hours, 6 hours, and 24 hours, respectively). B-D, are quantitated data by densitometry and normalized to actin. Data are shown as box and whiskers, center line is the median of the data set, box represents 75% of the data, and bars indicate whiskers from minimum to maximum. Data were analyzed by Student’s t test between control group and 50 or 80 cm H2O pressure treated groups. *P < .05, **P < .001
Article Snippet:
Techniques: Expressing, Western Blot, Control
Journal: BMC Immunology
Article Title: Expression of P2 receptors in human B cells and Epstein-Barr virus-transformed lymphoblastoid cell lines
doi: 10.1186/1471-2172-7-22
Figure Lengend Snippet: P2 receptors (P2X 1 , P2X 4 , P2X 7 , P2Y 1 , and P2Y 11 ) compared by Western blotting in B cells and LCLs . Proteins extracted from B cells and LCLs were probed with rabbit polyclonal antibodies directed against P2X 1 (60-kDa), P2X 4 (65-kDa), P2X 7 (68-kDa), P2Y 1 (66-kDa), and P2Y 11 (50-kDa) (left). Membranes were re-probed by GAPDH (40-kDa) (right). Data are representatives of 4 independent experiments.
Article Snippet: The membrane was incubated with
Techniques: Western Blot
Journal: BioMed Research International
Article Title: Retrograde Labeling of Different Distribution Features of DRG P2X2 and P2X3 Receptors in a Neuropathic Pain Rat Model
doi: 10.1155/2020/9861459
Figure Lengend Snippet: Expression and size distribution of P2X1-positive cells to total and FG-positive DRG neurons between the control and CCI groups. (a) P2X1-positive fluorescence images of total and FG-positive DRG between the control and CCI groups (200x, scale is 50 μ m). (b) The percentages of the expression and size distribution of P2X1-positive cells to total and FG-positive DRG neurons between the control and CCI groups. There was no significant difference between the control and CCI groups, n = 6.
Article Snippet: The primary antibodies were
Techniques: Expressing, Fluorescence